FastQCFastQC Report
Wed 25 May 2016
SRR2049416_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049416_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5627185
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219830.39065714029305953No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196490.3491799185560809No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124920.22199376775421462No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111300.059.730421
GTATCAA213500.041.7549481
GTGGTAT42450.033.6947171
ATCAACG273400.032.2780953
TCAACGC278250.031.7325464
TGGTATC42950.031.5176622
TATCAAC288950.030.7226662
CAACGCA288500.030.6545245
AACGCAG308350.028.8503256
CGCAGAG345500.025.4040558
ACGCAGA349800.025.2547027
GTACATG186950.022.800271
TATAACG4400.022.669342
GTACACG4250.022.3621771
GCAGAGT388200.022.2430939
TACATGG180700.021.6854322
AGAGTAC350650.020.8256310-11
ACATGGG183650.020.741693
GAGTACT265400.020.47320412-13
CAGAGTA368050.020.11852510-11
Produced by FastQC (version 0.11.5)