##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049416_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5627185 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12031362039812 33.0 31.0 34.0 30.0 34.0 2 32.302416394698234 34.0 31.0 34.0 30.0 34.0 3 32.37486274220591 34.0 31.0 34.0 30.0 34.0 4 35.99439879797803 37.0 35.0 37.0 35.0 37.0 5 35.89107057969482 37.0 35.0 37.0 35.0 37.0 6 35.91840485073798 37.0 35.0 37.0 35.0 37.0 7 35.84939716750027 37.0 35.0 37.0 35.0 37.0 8 35.84219960779679 37.0 35.0 37.0 35.0 37.0 9 37.4630014829795 39.0 37.0 39.0 35.0 39.0 10-11 37.46410203325463 39.0 37.0 39.0 34.5 39.0 12-13 37.45175269695238 39.0 37.0 39.0 35.0 39.0 14-15 38.689013067812766 40.0 38.0 41.0 34.0 41.0 16-17 38.67766378748877 40.0 38.0 41.0 34.0 41.0 18-19 38.724894152227094 40.0 38.0 41.0 34.0 41.0 20-21 38.725304659434514 40.0 38.0 41.0 34.0 41.0 22-23 38.641473134435785 40.0 38.0 41.0 34.0 41.0 24-25 38.546003108126 40.0 38.0 41.0 34.0 41.0 26-27 38.37196573419925 40.0 38.0 41.0 34.0 41.0 28-29 38.18541730900974 40.0 38.0 41.0 34.0 41.0 30-31 37.98917664871512 40.0 37.5 41.0 33.0 41.0 32-33 37.73057159130187 40.0 37.0 41.0 33.0 41.0 34-35 37.53664052274805 40.0 37.0 41.0 33.0 41.0 36-37 37.33539211879474 40.0 36.0 41.0 32.5 41.0 38-39 37.461114749204086 40.0 37.0 41.0 33.0 41.0 40-41 37.360112738429606 40.0 37.0 41.0 33.0 41.0 42-43 37.15982796016125 40.0 37.0 41.0 32.0 41.0 44-45 36.91496902980798 40.0 36.0 41.0 31.0 41.0 46-47 36.57244181593461 40.0 36.0 41.0 30.5 41.0 48-49 36.28558764284452 39.5 36.0 41.0 30.0 41.0 50-51 36.06434700476348 39.0 36.0 41.0 29.5 41.0 52-53 35.89167523370922 39.0 35.0 41.0 29.0 41.0 54-55 35.791264459938674 39.0 35.0 41.0 28.5 41.0 56-57 35.69849027533305 39.0 35.0 41.0 28.0 41.0 58-59 35.47703469852155 39.0 35.0 40.5 28.0 41.0 60-61 35.24392418233984 38.5 35.0 40.0 27.5 41.0 62-63 34.97318739298601 38.0 34.5 40.0 27.0 41.0 64-65 34.7261102131883 38.0 34.0 40.0 26.0 41.0 66-67 34.3980393393855 37.0 34.0 40.0 26.0 41.0 68-69 34.08324899572344 37.0 34.0 40.0 26.0 41.0 70-71 33.756857114169875 36.0 34.0 39.0 25.5 41.0 72-73 33.393374573610075 36.0 33.0 39.0 25.0 40.5 74-75 33.02125414394587 35.0 33.0 38.5 24.0 40.0 76-77 30.971231530507705 33.5 30.0 36.0 21.0 38.5 78-79 32.275725340467744 35.0 32.5 37.0 23.5 39.0 80-81 32.272722506901765 35.0 33.0 37.0 24.0 39.0 82-83 31.991862005603153 35.0 33.0 36.0 23.0 39.0 84-85 31.714773994457264 35.0 33.0 36.0 22.5 37.0 86-87 31.437192041846856 35.0 32.5 36.0 21.5 37.0 88-89 31.168696604074682 35.0 32.0 35.0 20.0 37.0 90-91 30.896919418856854 35.0 32.0 35.0 19.5 36.0 92-93 30.655819295082708 35.0 32.0 35.0 18.0 36.0 94-95 30.39665543251199 34.5 32.0 35.0 12.5 36.0 96-97 30.162911118081244 34.0 31.5 35.0 4.5 35.5 98-99 29.88911214043967 34.0 31.0 35.0 2.0 35.0 100-101 29.298577174910726 34.0 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3694615540975903 1101 2 0.3672521954128527 1101 3 0.3630275466452275 1101 4 0.30411714705809345 1101 5 0.2665347171818624 1101 6 0.2750137604307312 1101 7 0.335896449640984 1101 8 0.30858932442006193 1101 9 0.4378399716298276 1101 10-11 0.3929721170356686 1101 12-13 0.3561076626193156 1101 14-15 0.4555124221786997 1101 16-17 0.42306395290985677 1101 18-19 0.40735249672251683 1101 20-21 0.3455518711368484 1101 22-23 0.29524415977731167 1101 24-25 0.3187603763058604 1101 26-27 0.3031605022657047 1101 28-29 0.32818278937726575 1101 30-31 0.30172908700485124 1101 32-33 0.2617954984467801 1101 34-35 0.26532686290815377 1101 36-37 0.2640930622099873 1101 38-39 0.3444975383018445 1101 40-41 0.4130700843896946 1101 42-43 0.3196292173089006 1101 44-45 0.281250912647117 1101 46-47 0.23161523098364967 1101 48-49 0.16825482398972724 1101 50-51 0.26008778518610853 1101 52-53 0.2386731419215522 1101 54-55 0.17945101627294946 1101 56-57 0.16873261427588915 1101 58-59 0.1873470696150079 1101 60-61 0.23620193215040075 1101 62-63 0.31207774121470067 1101 64-65 0.1997281122698169 1101 66-67 0.15523152806878926 1101 68-69 0.1684716873761758 1101 70-71 0.10070944194266929 1101 72-73 0.061631718846264505 1101 74-75 -0.0045551148658944385 1101 76-77 0.055902043007417745 1101 78-79 0.18353300153773944 1101 80-81 0.16458227199023412 1101 82-83 0.06707694577913159 1101 84-85 -0.042667835983653646 1101 86-87 -0.075518825983373 1101 88-89 -0.10429929586158337 1101 90-91 -0.03811090076555246 1101 92-93 -0.12909310460723944 1101 94-95 -0.14935265933082675 1101 96-97 -0.16974603692169765 1101 98-99 -0.2441826627094379 1101 100-101 -0.29373397124323475 1102 1 0.3628946077276538 1102 2 0.3551407479813946 1102 3 0.3178506011763744 1102 4 0.2990684498980585 1102 5 0.31345724402765995 1102 6 0.3035983422823989 1102 7 0.32404763610885823 1102 8 0.29711420101509844 1102 9 0.3845428722509183 1102 10-11 0.3410257553393379 1102 12-13 0.3302234572461984 1102 14-15 0.36848019073560323 1102 16-17 0.4179384710480818 1102 18-19 0.3802312884307568 1102 20-21 0.33486427168760713 1102 22-23 0.30687872476107714 1102 24-25 0.3055122680391591 1102 26-27 0.30687099591852984 1102 28-29 0.29641693079518205 1102 30-31 0.26958665371825674 1102 32-33 0.24975170122213086 1102 34-35 0.26433360677526707 1102 36-37 0.18224084487659553 1102 38-39 0.4024177910018025 1102 40-41 0.3542826384549187 1102 42-43 0.3219736232068513 1102 44-45 0.3308912951365741 1102 46-47 0.29543042655250673 1102 48-49 0.14684301843112024 1102 50-51 0.30325424892505026 1102 52-53 0.2672491387215956 1102 54-55 0.3424412089846953 1102 56-57 0.3114983827492708 1102 58-59 0.3791845810983716 1102 60-61 0.34580065859575626 1102 62-63 0.2319783853820283 1102 64-65 0.31782827193804053 1102 66-67 0.30649656633202227 1102 68-69 0.21337061453318285 1102 70-71 0.1841582384969982 1102 72-73 0.14985878343442494 1102 74-75 0.15432991219968528 1102 76-77 0.1562405206868327 1102 78-79 0.2696900041377077 1102 80-81 0.26117859434641133 1102 82-83 0.3037714206871591 1102 84-85 0.27926600252617106 1102 86-87 0.24602044355373565 1102 88-89 0.16329192134214665 1102 90-91 0.10488333310169295 1102 92-93 0.22168465299376194 1102 94-95 0.10149815362548509 1102 96-97 0.051585625462976736 1102 98-99 0.0793120068663491 1102 100-101 0.02819087013248378 1103 1 0.3360924361874069 1103 2 0.37339724663222285 1103 3 0.2828681745891046 1103 4 0.27891899804766496 1103 5 0.30430903641262574 1103 6 0.30283320379732714 1103 7 0.22311652559698558 1103 8 0.31083098025435163 1103 9 0.35061991588349173 1103 10-11 0.3689798449413857 1103 12-13 0.3165183135429217 1103 14-15 0.3975201875396621 1103 16-17 0.36630107207896856 1103 18-19 0.31052739438078447 1103 20-21 0.32791242244029206 1103 22-23 0.3040763720132702 1103 24-25 0.34969305091196645 1103 26-27 0.27735712436871296 1103 28-29 0.2990074123457944 1103 30-31 0.3161442355216195 1103 32-33 0.38531157024875284 1103 34-35 0.38733724744145803 1103 36-37 0.3834127995598209 1103 38-39 0.39556446031086523 1103 40-41 0.48316674518748215 1103 42-43 0.5475901417905149 1103 44-45 0.5911875694999722 1103 46-47 0.5436107135850179 1103 48-49 0.5081802799736153 1103 50-51 0.5561550386336123 1103 52-53 0.5534343337635335 1103 54-55 0.5297987471050263 1103 56-57 0.5152407954169931 1103 58-59 0.4555850443265186 1103 60-61 0.5187713705319439 1103 62-63 0.5479291581545951 1103 64-65 0.49917634131051614 1103 66-67 0.40136582370544005 1103 68-69 0.43852752128616856 1103 70-71 0.49333167874279127 1103 72-73 0.45522578782211554 1103 74-75 0.4629031676738222 1103 76-77 0.4000500891641323 1103 78-79 0.4901022232716983 1103 80-81 0.45631431691712265 1103 82-83 0.451782063889798 1103 84-85 0.39055871285798105 1103 86-87 0.4663305865621403 1103 88-89 0.3908876886572763 1103 90-91 0.4268878320885712 1103 92-93 0.3791799062499308 1103 94-95 0.41505270770899116 1103 96-97 0.3494878868302074 1103 98-99 0.33401419948437194 1103 100-101 0.3410237761984902 1104 1 0.2766524857306081 1104 2 0.30797450281461636 1104 3 0.27910718133702517 1104 4 0.20289597650980085 1104 5 0.2597111289927625 1104 6 0.27884784010756647 1104 7 0.2985758735000772 1104 8 0.2776243380132257 1104 9 0.4136038133943387 1104 10-11 0.31294964937544734 1104 12-13 0.26872912348935785 1104 14-15 0.37267296125317273 1104 16-17 0.35248541132588684 1104 18-19 0.32775048079483327 1104 20-21 0.30558618346586286 1104 22-23 0.2667823115875336 1104 24-25 0.3244363079815571 1104 26-27 0.3065849665948548 1104 28-29 0.37244118587505426 1104 30-31 0.3201310690029828 1104 32-33 0.3131571618912048 1104 34-35 0.31031379789571645 1104 36-37 0.34534847706814986 1104 38-39 0.38425633437881146 1104 40-41 0.38406661701328204 1104 42-43 0.39493769084171504 1104 44-45 0.33217915403989196 1104 46-47 0.44142421319303793 1104 48-49 0.34290240474847167 1104 50-51 0.3841259143232705 1104 52-53 0.4475292891459475 1104 54-55 0.3612497774707677 1104 56-57 0.3727806742095723 1104 58-59 0.3467240168300094 1104 60-61 0.3613451391441558 1104 62-63 0.3557443043855457 1104 64-65 0.3260028045564951 1104 66-67 0.3780206654898848 1104 68-69 0.3293470565468013 1104 70-71 0.3095420825475941 1104 72-73 0.33749666908291687 1104 74-75 0.3040033418621917 1104 76-77 0.29718535505475785 1104 78-79 0.4464390025342908 1104 80-81 0.3791044814444078 1104 82-83 0.37401131182994973 1104 84-85 0.30185821588588624 1104 86-87 0.26286289564277254 1104 88-89 0.28477710138521317 1104 90-91 0.3180514278795279 1104 92-93 0.3415147852412126 1104 94-95 0.2686015699134643 1104 96-97 0.24853511771811654 1104 98-99 0.20748729421710266 1104 100-101 0.2347094050782026 1105 1 0.28759889465324306 1105 2 0.2546535722815051 1105 3 0.23780705740186647 1105 4 0.19065317848065888 1105 5 0.21821086305489246 1105 6 0.20436177673147427 1105 7 0.19190000036318366 1105 8 0.24612761316827658 1105 9 0.23050547902021634 1105 10-11 0.1808518269464301 1105 12-13 0.22174964913957496 1105 14-15 0.2538401249169695 1105 16-17 0.2536441366537474 1105 18-19 0.28664827059206033 1105 20-21 0.2166018637422482 1105 22-23 0.1753948023177685 1105 24-25 0.19785316060047364 1105 26-27 0.25590240097182715 1105 28-29 0.24322403727993702 1105 30-31 0.3115499535568489 1105 32-33 0.2759531249338565 1105 34-35 0.27070669518501234 1105 36-37 0.24143691253703992 1105 38-39 0.27706348705228123 1105 40-41 0.22291620375269616 1105 42-43 0.2635137344969962 1105 44-45 0.2732248311676031 1105 46-47 0.3290375462713584 1105 48-49 0.32680445147046555 1105 50-51 0.21642387566018328 1105 52-53 0.21992797077743376 1105 54-55 0.3186391520503804 1105 56-57 0.2730319209732457 1105 58-59 0.2207298910457567 1105 60-61 0.33643074031508746 1105 62-63 0.31252740374740284 1105 64-65 0.31682086077563554 1105 66-67 0.317430560087125 1105 68-69 0.2816397734019134 1105 70-71 0.19683924882527037 1105 72-73 0.2648869795360653 1105 74-75 0.2893057481651269 1105 76-77 0.32928069543821437 1105 78-79 0.4184062322291595 1105 80-81 0.34992096425711594 1105 82-83 0.37289125008705426 1105 84-85 0.3753320565183813 1105 86-87 0.3623554596880574 1105 88-89 0.3924098618859517 1105 90-91 0.3654177856646008 1105 92-93 0.3612005168030912 1105 94-95 0.42897572497346914 1105 96-97 0.37576311724696154 1105 98-99 0.4088731050497003 1105 100-101 0.5437633347288617 1106 1 0.33972410098095907 1106 2 0.2646442170625889 1106 3 0.28684963218330495 1106 4 0.22983047097351061 1106 5 0.21874551832535616 1106 6 0.24776004338357893 1106 7 0.23957132730846808 1106 8 0.21837447323106574 1106 9 0.34216938071105574 1106 10-11 0.23869196508297108 1106 12-13 0.22625884468281043 1106 14-15 0.31775539747334136 1106 16-17 0.34100823405300673 1106 18-19 0.34561014869134965 1106 20-21 0.29635121178394996 1106 22-23 0.2711847759211139 1106 24-25 0.2625095760876661 1106 26-27 0.2992069032204725 1106 28-29 0.3106199836804677 1106 30-31 0.38514652170167096 1106 32-33 0.44062584035378904 1106 34-35 0.4972918948523244 1106 36-37 0.530340315914934 1106 38-39 0.5008073865535678 1106 40-41 0.5686842303376594 1106 42-43 0.611751795404821 1106 44-45 0.6658903982103723 1106 46-47 0.6423236735680646 1106 48-49 0.6999827358156381 1106 50-51 0.7702353588707425 1106 52-53 0.6836114054238607 1106 54-55 0.7174473095815657 1106 56-57 0.656114013869626 1106 58-59 0.6859103417246999 1106 60-61 0.7258281287119601 1106 62-63 0.6366456368371942 1106 64-65 0.6321782311055841 1106 66-67 0.6475483574509724 1106 68-69 0.6670055733218234 1106 70-71 0.6314836651527074 1106 72-73 0.6940263065738534 1106 74-75 0.5844694117546112 1106 76-77 0.5045808390542597 1106 78-79 0.5381223100577657 1106 80-81 0.6262075393572459 1106 82-83 0.5823585244065619 1106 84-85 0.5613895627934511 1106 86-87 0.5546506003825655 1106 88-89 0.5766272983075531 1106 90-91 0.5474171766718996 1106 92-93 0.5523731997511128 1106 94-95 0.5866822554667834 1106 96-97 0.6462222869453029 1106 98-99 0.6691329109353816 1106 100-101 0.7614810339373363 1107 1 0.22482926331145592 1107 2 0.22272814613035052 1107 3 0.21005754109470587 1107 4 0.17058405017351674 1107 5 0.16779399138773243 1107 6 0.15849494849660317 1107 7 0.16743641974965584 1107 8 0.18551469284219024 1107 9 0.2626986625218848 1107 10-11 0.19636649547928187 1107 12-13 0.20691118312569046 1107 14-15 0.17571803329603597 1107 16-17 0.19883937072651747 1107 18-19 0.21253313050023337 1107 20-21 0.20176099015730387 1107 22-23 0.22302522804871217 1107 24-25 0.2692242457311522 1107 26-27 0.3027642798018775 1107 28-29 0.2292039386404241 1107 30-31 0.2762639126981554 1107 32-33 0.2545034392548118 1107 34-35 0.23725033447828991 1107 36-37 0.21930418565559506 1107 38-39 0.2645277224365188 1107 40-41 0.3123779402849607 1107 42-43 0.2856898567389763 1107 44-45 0.221823765130587 1107 46-47 0.2293959937218375 1107 48-49 0.18130007345759225 1107 50-51 0.24018280601328712 1107 52-53 0.25855393304887286 1107 54-55 0.20623225895288755 1107 56-57 0.19277626351358634 1107 58-59 0.25140544809935506 1107 60-61 0.3166296499955692 1107 62-63 0.2890936875019605 1107 64-65 0.3141871848237656 1107 66-67 0.3085776259005186 1107 68-69 0.297497055543694 1107 70-71 0.30041923313618213 1107 72-73 0.30270470691009876 1107 74-75 0.31292353281867946 1107 76-77 0.32766226118359043 1107 78-79 0.4055851630578715 1107 80-81 0.4110173041730576 1107 82-83 0.45262430215306537 1107 84-85 0.45857407693429053 1107 86-87 0.38775450720568116 1107 88-89 0.35745878358214256 1107 90-91 0.4734825726632437 1107 92-93 0.4331987668091095 1107 94-95 0.5246376807276327 1107 96-97 0.5365983760217077 1107 98-99 0.5142757535846485 1107 100-101 0.6089308034514858 1108 1 0.21234725886267114 1108 2 0.19657132548194767 1108 3 0.12691467212147245 1108 4 0.15353292914679884 1108 5 0.0504775319263473 1108 6 0.0893633572518695 1108 7 0.13016200319140836 1108 8 0.1362687525624935 1108 9 0.21826557273467984 1108 10-11 0.07811014080686363 1108 12-13 0.1165462639088446 1108 14-15 0.13834718508905297 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-0.8265009649665842 2316 60-61 -0.7693835889538221 2316 62-63 -1.1974836210862705 2316 64-65 -1.4540538128715923 2316 66-67 -1.0249881434710701 2316 68-69 -1.0477410328916577 2316 70-71 -0.8170160683796439 2316 72-73 -0.9800144954168744 2316 74-75 -0.775013684416912 2316 76-77 -0.7486538344134175 2316 78-79 -0.7391682301150908 2316 80-81 -0.6293147974560753 2316 82-83 -0.7476722429118929 2316 84-85 -0.8863650990824006 2316 86-87 -0.9658061492261254 2316 88-89 -0.7303807735104719 2316 90-91 -0.6743429517362145 2316 92-93 -0.6663122522019655 2316 94-95 -0.9239269342188834 2316 96-97 -0.817862419036345 2316 98-99 -0.7965071071554988 2316 100-101 -1.0779931863993681 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 12.0 4 36.0 5 85.0 6 234.0 7 652.0 8 1851.0 9 3966.0 10 6851.0 11 10449.0 12 15407.0 13 21883.0 14 26596.0 15 33083.0 16 41234.0 17 53458.0 18 45161.0 19 25902.0 20 20161.0 21 19569.0 22 20116.0 23 22018.0 24 25548.0 25 31040.0 26 39214.0 27 49177.0 28 62961.0 29 80230.0 30 101146.0 31 128289.0 32 165842.0 33 219142.0 34 304257.0 35 456302.0 36 723199.0 37 1171644.0 38 1347493.0 39 352834.0 40 142.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.920862631402365 22.413491759676386 16.19047432778834 22.475171281132912 2 14.470406570555797 22.265673107527345 39.832963778666695 23.430956543250158 3 16.414725475829513 27.40299614033548 32.201871170479386 23.980407213355623 4 11.819716255441897 19.167009941402203 39.301859157012025 29.711414646143876 5 11.88778305567645 38.30531173733066 36.02805316979884 13.778852037194051 6 28.1347064073351 39.792666963090284 18.44336016429119 13.629266465283427 7 24.831456580865922 34.14915272911767 23.531641486818007 17.4877492031984 8 24.731086680107374 35.593480576878136 21.03735704441919 18.6380756985953 9 24.485465985709716 16.833674564111075 22.71459642566906 35.96626302451015 10-11 22.684947335105328 27.815205227908884 29.566712154198648 19.93313528278714 12-13 23.855657846685332 25.654843407494155 29.75143877444939 20.73805997137112 14-15 21.119934005704128 27.532599522351713 28.03839703665424 23.309069435289924 16-17 19.70968610415332 30.725033920157234 28.594279377699504 20.971000597989935 18-19 19.672194802248764 28.6853538719681 31.9920080800635 19.65044324571964 20-21 20.66100501700814 27.87660948238796 31.874595321955123 19.587790178648778 22-23 20.288954423925993 27.88810924112145 32.01126318043569 19.811673154516868 24-25 20.06362544740669 28.00439334220816 31.860829752036157 20.071151458348993 26-27 19.92134547804033 28.07644045856953 31.83149765535521 20.170716408034927 28-29 19.962088330174176 27.920846504389246 32.10163355897222 20.015431606464357 30-31 20.223805388226058 28.311298086534837 31.654064307485125 19.810832217753983 32-33 19.761724417626773 28.130516008732485 31.984248962643804 20.123510610996938 34-35 20.1415972517625 28.01124311290023 31.697529303635935 20.14963033170133 36-37 20.55045015585736 28.08597568127615 31.261849246659818 20.101724916206678 38-39 20.207702174186373 28.360965497447815 31.387108425866316 20.04422390249949 40-41 19.99590373692928 28.137382088045666 31.53537891766245 20.33133525736261 42-43 20.052311048860876 28.301724002349697 31.180217836528684 20.465747112260743 44-45 20.224444448985928 28.592640824461668 30.831718465788605 20.351196260763796 46-47 20.52274788909516 28.724903918920923 30.31486551314578 20.437482678838137 48-49 20.444937656587154 28.644080085899237 30.286998996633763 20.62398326087984 50-51 20.489753115303344 28.736584676246675 29.85757940523721 20.916082803212774 52-53 20.458499960992114 28.854072645660693 29.795317880522838 20.89210951282435 54-55 20.57619831024361 29.333733773402752 29.60365762907195 20.48641028728169 56-57 20.581124838351336 28.872890526024346 29.60139132029529 20.94459331532903 58-59 20.41741253065721 28.63166974786317 29.83696166953639 21.113956051943234 60-61 20.661108115046396 28.867236064100442 29.623719448914848 20.847936371938317 62-63 20.43444823616753 28.8629643830285 29.744940596914265 20.957646783889704 64-65 20.478843359003058 28.98475455126084 29.619786100248046 20.916615989488054 66-67 20.38449156287257 28.8518666320827 29.63044120943993 21.133200595604798 68-69 20.676785000222154 29.11918069933798 29.302483671746565 20.901550628693297 70-71 20.785822925360712 28.750937546122024 29.27600780265846 21.187231725858805 72-73 20.395837648346486 28.944783914359572 29.42129464877948 21.238083788514466 74-75 20.41985621401485 29.030096815801887 29.341640817960062 21.2084061522232 76-77 20.475272168706613 29.27961361067006 29.154264194841705 21.09085002578162 78-79 20.492552804963665 29.13295710906502 29.365681120920968 21.008808965050346 80-81 20.450307065510035 29.113547597375433 29.23547458761452 21.200670749500013 82-83 20.24733896297726 29.025258049041113 29.516605708031374 21.210797279950256 84-85 20.1629507602414 29.03109206064871 29.32234910683186 21.483608072278024 86-87 20.30877862571679 29.12326771089256 29.2365270006468 21.33142666274385 88-89 20.151712144115606 29.04478341891418 29.30634198667221 21.497162450298006 90-91 20.02881757317506 28.897525235813266 29.396759124824378 21.6768980661873 92-93 20.173982396328817 28.99683207134801 29.210180554961955 21.61900497736121 94-95 20.075205059472403 28.78283101848396 29.38810210785286 21.753861814190774 96-97 20.01623197327322 28.962734435303172 29.241382717462283 21.779650873961323 98-99 19.857997539076578 28.83389939784858 29.28357593071882 22.024527132356024 100-101 19.822166856074574 28.97787259526744 29.024654423126307 22.175306125531684 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3216.0 1 2227.0 2 1836.0 3 2351.0 4 1818.5 5 1525.5 6 2543.0 7 4935.0 8 7363.5 9 8619.0 10 7894.0 11 6645.5 12 6665.0 13 7179.5 14 7782.5 15 8303.0 16 8865.0 17 9493.5 18 10288.5 19 11355.5 20 12913.0 21 15177.5 22 19207.0 23 25984.0 24 33727.0 25 42029.5 26 54399.0 27 70765.5 28 90282.5 29 108566.0 30 121606.0 31 135760.0 32 152082.5 33 166907.5 34 179575.5 35 190114.5 36 196788.5 37 202070.0 38 209010.5 39 211875.0 40 213289.0 41 210079.0 42 202835.0 43 198867.5 44 192521.5 45 184758.5 46 178620.5 47 172992.0 48 167032.0 49 162843.0 50 158061.0 51 146421.5 52 129543.5 53 116582.5 54 109450.0 55 104659.0 56 98485.5 57 88457.0 58 78210.5 59 69276.0 60 59627.0 61 48918.0 62 39030.0 63 30848.0 64 24549.5 65 19734.0 66 15751.0 67 12231.5 68 9290.0 69 6989.0 70 5086.5 71 3722.5 72 2836.0 73 2105.5 74 1591.5 75 1173.0 76 794.5 77 564.0 78 407.5 79 272.0 80 174.5 81 113.5 82 77.0 83 49.0 84 37.5 85 25.5 86 18.0 87 14.5 88 9.5 89 7.0 90 4.0 91 2.5 92 3.5 93 2.0 94 0.5 95 0.5 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.052459622351140045 2 0.002523464218787902 3 2.132504973623579E-4 4 1.954796225821614E-4 5 0.002772256465710653 6 0.0018304001023602386 7 0.0 8 0.0 9 1.0662524868117895E-4 10-11 3.5541749560392986E-5 12-13 0.0 14-15 5.686679929662878E-4 16-17 0.0 18-19 2.6656312170294738E-5 20-21 4.7092818167520707E-4 22-23 0.0 24-25 6.042097425266807E-4 26-27 0.007579278093753805 28-29 0.007490423719852822 30-31 0.0011373359859325756 32-33 0.006770703291254864 34-35 0.008023549963258717 36-37 0.004353864321148141 38-39 0.0024434952822770177 40-41 0.0018659418519206316 42-43 0.001341701045904835 44-45 8.263456772791369E-4 46-47 6.48636929477172E-4 48-49 0.0022569010970849546 50-51 0.0015016389189266037 52-53 0.0020969632240631863 54-55 7.55262178158351E-4 56-57 2.132504973623579E-4 58-59 7.463767407682527E-4 60-61 0.006388629483480639 62-63 0.00288776715178193 64-65 0.0011195651111523789 66-67 0.005322376996668849 68-69 0.007730330529385474 70-71 0.0022835574092552493 72-73 5.242408060157966E-4 74-75 0.0037496545786214596 76-77 0.0020347651623324986 78-79 0.0023724117831562318 80-81 8.352311146692352E-4 82-83 1.5105243563167019E-4 84-85 4.4427186950491234E-4 86-87 0.004860334252383741 88-89 0.0026123185926888847 90-91 0.0011195651111523789 92-93 1.1551068607127719E-4 94-95 6.575223668672703E-4 96-97 3.4653205821383163E-4 98-99 5.153553686256983E-4 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5627185.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.22933100016355 #Duplication Level Percentage of deduplicated Percentage of total 1 74.56318805650933 40.43511805543886 2 15.512898133704315 16.825081753289414 3 4.610523401618664 7.500767988911357 4 1.8536822752587372 4.020957986965694 5 0.9322442326746432 2.5277490533353357 6 0.5583388374812089 1.8167004976809003 7 0.35908756375958284 1.363115484721253 8 0.24252403463150468 1.0521532919621597 9 0.18200850380213066 0.8883179457777243 >10 1.027928774195076 10.322344281487146 >50 0.09256993088537467 3.4929254101810407 >100 0.05923784664508333 6.007318998915915 >500 0.004285109874302391 1.5432071726301857 >1k 0.0013514501634917706 1.1373619568507725 >5k 3.296219910857138E-5 0.09940387631099144 >10k+ 9.888659732571413E-5 0.9674762455414676 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21983 0.39065714029305953 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19649 0.3491799185560809 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12492 0.22199376775421462 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 1.7770874780196493E-5 0.0 1.7770874780196493E-5 0.0 4 0.0 1.7770874780196493E-5 0.0 5.3312624340589476E-5 0.0 5 0.0 1.7770874780196493E-5 0.0 5.3312624340589476E-5 0.0 6 0.0 1.7770874780196493E-5 0.0 5.3312624340589476E-5 0.0 7 0.0 1.7770874780196493E-5 0.0 5.3312624340589476E-5 0.0 8 0.0 1.7770874780196493E-5 0.0 5.3312624340589476E-5 0.0 9 0.0 1.7770874780196493E-5 0.0 7.108349912078597E-5 0.0 10-11 0.0 1.7770874780196493E-5 0.0 8.885437390098247E-5 0.0 12-13 0.0 2.6656312170294738E-5 0.0 1.155106860712772E-4 0.0 14-15 0.0 4.4427186950491234E-5 0.0 1.2439612346137546E-4 0.0 16-17 0.0 5.3312624340589476E-5 0.0 1.332815608514737E-4 0.0 18-19 0.0 6.219806173068772E-5 0.0 1.4216699824157194E-4 0.0 20-21 0.0 7.108349912078597E-5 0.0 1.9547962258216142E-4 0.0 22-23 0.0 7.108349912078597E-5 0.0 5.064699312356E-4 0.0 24-25 0.0 7.108349912078597E-5 0.0 0.0010129398624712002 0.0 26-27 0.0 7.108349912078597E-5 0.0 0.0020525360371126946 0.0 28-29 0.0 7.108349912078597E-5 0.0 0.004824792502823348 0.0 30-31 0.0 7.108349912078597E-5 0.0 0.01135558898454556 0.0 32-33 0.0 7.996893651088423E-5 0.0 0.024861453817494893 0.0 34-35 0.0 8.885437390098247E-5 0.0 0.04552898118686341 0.0 36-37 0.0 8.885437390098247E-5 0.0 0.07653027224091619 0.0 38-39 0.0 8.885437390098247E-5 0.0 0.1175809929831701 0.0 40-41 0.0 8.885437390098247E-5 0.0 0.16087084394772874 0.0 42-43 0.0 8.885437390098247E-5 0.0 0.2051914056495388 0.0 44-45 0.0 8.885437390098247E-5 0.0 0.2583707484292768 0.0 46-47 0.0 8.885437390098247E-5 0.0 0.32344769187435635 0.0 48-49 0.0 8.885437390098247E-5 0.0 0.3909059325399823 0.0 50-51 0.0 1.4216699824157194E-4 0.0 0.4590572373220358 0.0 52-53 0.0 1.4216699824157194E-4 0.0 0.526266685740739 0.0 54-55 0.0 1.5993787302176845E-4 0.0 0.6011798083766573 0.0 56-57 0.0 1.7770874780196494E-4 0.0 0.6902030055880515 0.0 58-59 0.0 1.7770874780196494E-4 0.0 0.7887869334311917 0.0 60-61 0.0 1.7770874780196494E-4 0.0 0.888570395321995 0.0 62-63 0.0 1.7770874780196494E-4 0.0 0.9881406067154359 0.0 64-65 0.0 2.0436505997225966E-4 0.0 1.0968183914337275 0.0 66-67 0.0 2.310213721425544E-4 0.0 1.2246709500398514 0.0 68-69 0.0 2.310213721425544E-4 0.0 1.3679575133925754 0.0 70-71 0.0 2.310213721425544E-4 0.0 1.5199695762623762 0.0 72-73 0.0 2.487922469227509E-4 0.0 1.6822443193177405 0.0 74-75 0.0 2.5767768431284916E-4 0.0 1.8491661461281264 0.0 76-77 3.5541749560392986E-5 2.665631217029474E-4 0.0 2.028296563912507 0.0 78-79 3.5541749560392986E-5 2.665631217029474E-4 0.0 2.2338256161828696 0.0 80-81 3.5541749560392986E-5 2.665631217029474E-4 0.0 2.462056960984933 0.0 82-83 3.5541749560392986E-5 2.665631217029474E-4 0.0 2.6991915140518747 0.0 84-85 3.5541749560392986E-5 2.665631217029474E-4 0.0 2.9399779818861473 0.0 86-87 3.5541749560392986E-5 2.665631217029474E-4 0.0 3.1989351691831707 0.0 88-89 3.5541749560392986E-5 2.665631217029474E-4 0.0 3.4879429057335063 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11130 0.0 59.73042 1 GTATCAA 21350 0.0 41.754948 1 GTGGTAT 4245 0.0 33.694717 1 ATCAACG 27340 0.0 32.278095 3 TCAACGC 27825 0.0 31.732546 4 TGGTATC 4295 0.0 31.517662 2 TATCAAC 28895 0.0 30.722666 2 CAACGCA 28850 0.0 30.654524 5 AACGCAG 30835 0.0 28.850325 6 CGCAGAG 34550 0.0 25.404055 8 ACGCAGA 34980 0.0 25.254702 7 GTACATG 18695 0.0 22.80027 1 TATAACG 440 0.0 22.66934 2 GTACACG 425 0.0 22.362177 1 GCAGAGT 38820 0.0 22.243093 9 TACATGG 18070 0.0 21.685432 2 AGAGTAC 35065 0.0 20.82563 10-11 ACATGGG 18365 0.0 20.74169 3 GAGTACT 26540 0.0 20.473204 12-13 CAGAGTA 36805 0.0 20.118525 10-11 >>END_MODULE