FastQCFastQC Report
Wed 25 May 2016
SRR2049415_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049415_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10315257
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT449020.43529695867005547No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370200.3588858716753252No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205730.19944243754663601No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103460.10029803426128889No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA241850.054.2487071
GTATCAA461000.043.3597371
ATCAACG572600.034.5924873
TCAACGC578500.034.2403534
TATCAAC594050.033.4220852
CAACGCA600600.032.995765
AACGCAG638850.031.102446
CGCAGAG691650.028.2281258
ACGCAGA703000.028.0021257
TGGTATC108050.027.8704992
GTGGTAT112850.027.3183821
GCAGAGT740650.025.449859
GTACATG409600.023.8669661
TACATGG398500.023.2903752
GAGTACT474950.022.2641412-13
ACATGGG410400.021.9561943
AGAGTAC664050.021.73295610-11
GTACTTT484900.021.58956514-15
CAGAGTA716400.021.5008810-11
AGTACTT481950.020.52142112-13
Produced by FastQC (version 0.11.5)