##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049415_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10315257 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.42254313198401 31.0 31.0 34.0 30.0 34.0 2 31.45420351620905 31.0 31.0 34.0 29.0 34.0 3 31.46948331001351 33.0 31.0 34.0 28.0 34.0 4 35.0944697742383 37.0 35.0 37.0 32.0 37.0 5 35.119176865879346 37.0 35.0 37.0 32.0 37.0 6 35.213393616853175 37.0 35.0 37.0 33.0 37.0 7 35.15701004831969 37.0 35.0 37.0 32.0 37.0 8 35.13108136811327 37.0 35.0 37.0 32.0 37.0 9 36.70679024284126 39.0 37.0 39.0 33.0 39.0 10-11 36.643409320776016 39.0 37.0 39.0 32.5 39.0 12-13 36.600865833977764 39.0 37.0 39.0 32.0 39.0 14-15 37.75373337765603 40.0 38.0 41.0 32.5 41.0 16-17 37.74665144067666 40.0 38.0 41.0 32.5 41.0 18-19 37.79252014758333 40.0 38.0 41.0 33.0 41.0 20-21 37.762474846724615 40.0 38.0 41.0 32.5 41.0 22-23 37.66036633890944 40.0 38.0 41.0 32.0 41.0 24-25 37.55317506873556 40.0 37.5 41.0 32.0 41.0 26-27 37.40721307282989 40.0 37.0 41.0 32.0 41.0 28-29 37.25140493348833 40.0 37.0 41.0 32.0 41.0 30-31 37.05125786008046 40.0 37.0 41.0 31.0 41.0 32-33 36.791144757711805 40.0 36.0 41.0 30.5 41.0 34-35 36.581307135634134 39.0 36.0 41.0 30.0 41.0 36-37 36.38465692129628 39.0 35.5 41.0 30.0 41.0 38-39 36.170241274647836 39.0 35.0 41.0 30.0 41.0 40-41 35.94225102680427 39.0 35.0 40.0 29.0 41.0 42-43 35.69851560654281 39.0 35.0 40.0 28.5 41.0 44-45 35.39532994669934 39.0 35.0 40.0 27.0 41.0 46-47 35.012653926121274 38.0 35.0 40.0 25.5 41.0 48-49 34.69220102805001 38.0 34.0 40.0 24.0 41.0 50-51 33.3182732141332 36.5 32.5 39.0 22.5 40.0 52-53 33.599740316697876 37.5 33.5 39.0 23.0 40.0 54-55 34.6391802453395 38.5 34.5 40.0 24.0 41.0 56-57 34.74993492648802 39.0 35.0 40.0 24.5 41.0 58-59 34.5873889036405 38.5 34.0 40.0 24.0 41.0 60-61 34.332092743787186 38.0 34.0 40.0 23.0 41.0 62-63 34.06268234519024 38.0 34.0 40.0 22.5 41.0 64-65 33.78421817313907 37.0 34.0 40.0 22.0 41.0 66-67 33.463502266594034 37.0 33.5 40.0 21.5 41.0 68-69 33.1172556340574 36.0 33.0 39.5 20.0 41.0 70-71 32.78266542462296 36.0 33.0 39.0 19.5 41.0 72-73 32.5220896095948 35.0 33.0 39.0 18.5 40.0 74-75 32.31927265602786 35.0 33.0 38.5 18.0 40.0 76-77 31.98348834158955 35.0 32.5 37.0 16.0 39.5 78-79 31.611571626378286 35.0 32.0 37.0 12.0 39.0 80-81 31.272471980097055 35.0 32.0 36.5 8.5 39.0 82-83 30.93373635770781 35.0 32.0 36.0 7.0 38.0 84-85 30.617879321862752 35.0 31.5 36.0 2.0 37.0 86-87 30.32651702230977 35.0 31.0 35.0 2.0 37.0 88-89 30.0371276740851 35.0 31.0 35.0 2.0 36.5 90-91 29.773590081177815 34.5 31.0 35.0 2.0 36.0 92-93 29.52670476363313 34.0 31.0 35.0 2.0 36.0 94-95 29.26555426588014 34.0 30.5 35.0 2.0 36.0 96-97 29.010942674525705 34.0 30.0 35.0 2.0 35.0 98-99 28.695285585225847 34.0 30.0 35.0 2.0 35.0 100-101 28.163549778740364 34.0 29.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.36562684632185594 1101 2 0.39851236447845295 1101 3 0.38139784906721275 1101 4 0.3639826019736958 1101 5 0.3721002127997508 1101 6 0.43217004840939666 1101 7 0.40700921464524953 1101 8 0.38292977311794374 1101 9 0.4402569099332041 1101 10-11 0.4475653045139367 1101 12-13 0.4800190126408097 1101 14-15 0.558243328110045 1101 16-17 0.5356919880482423 1101 18-19 0.5180915086117963 1101 20-21 0.4578218474916653 1101 22-23 0.47559484836448007 1101 24-25 0.4819389554627662 1101 26-27 0.4907503192077485 1101 28-29 0.46420835449603715 1101 30-31 0.4316636085024328 1101 32-33 0.38201608157992695 1101 34-35 0.41478117733817754 1101 36-37 0.3632255924306307 1101 38-39 0.28518779012844675 1101 40-41 0.26157222961010973 1101 42-43 0.25108379732801467 1101 44-45 0.1897465892690846 1101 46-47 0.1914205137221643 1101 48-49 0.05404109104276955 1101 50-51 0.06319291828157958 1101 52-53 0.14730624281034466 1101 54-55 0.4150105447701833 1101 56-57 0.38061783240621594 1101 58-59 0.4050311664226811 1101 60-61 0.41586472290337895 1101 62-63 0.3328363871564406 1101 64-65 0.30625326225760574 1101 66-67 0.3276773193154696 1101 68-69 0.19751974832182384 1101 70-71 0.13948456926136288 1101 72-73 0.11712380762715213 1101 74-75 0.1219737675086705 1101 76-77 0.07937561909320578 1101 78-79 0.06138830948655993 1101 80-81 -0.008010403539046251 1101 82-83 -0.043079035515145137 1101 84-85 -0.08141461121315885 1101 86-87 -0.17127180998825509 1101 88-89 -0.19900511036043866 1101 90-91 -0.2618653326147715 1101 92-93 -0.2887285845639589 1101 94-95 -0.42007160346931727 1101 96-97 -0.3947632188043215 1101 98-99 -0.504024516875166 1101 100-101 -0.636964503051054 1102 1 0.3193810902952805 1102 2 0.3112150852732647 1102 3 0.3065898094940174 1102 4 0.31989033778479836 1102 5 0.2978374246362492 1102 6 0.36587734792212245 1102 7 0.3316757384616693 1102 8 0.34560902411367067 1102 9 0.381071522670986 1102 10-11 0.4308801899584651 1102 12-13 0.3451312454469502 1102 14-15 0.4730774972848266 1102 16-17 0.39392789794301564 1102 18-19 0.3653403351623652 1102 20-21 0.41905964275040475 1102 22-23 0.37497977511293357 1102 24-25 0.39960546582619116 1102 26-27 0.40294881616809874 1102 28-29 0.36394528770969714 1102 30-31 0.3080099613070004 1102 32-33 0.3034033776759131 1102 34-35 0.2629144773686036 1102 36-37 0.2087617760370719 1102 38-39 0.19969225968262094 1102 40-41 0.16653913524839226 1102 42-43 0.1564710076814393 1102 44-45 0.07250766930774688 1102 46-47 0.05061569897715401 1102 48-49 0.00945842070155578 1102 50-51 0.019978238992010233 1102 52-53 0.20668931207330843 1102 54-55 0.3012766764075536 1102 56-57 0.2779737480193205 1102 58-59 0.2560936732403434 1102 60-61 0.26375611219392425 1102 62-63 0.3122344662311818 1102 64-65 0.23564038848930124 1102 66-67 0.2054214664127585 1102 68-69 0.20047292331721422 1102 70-71 0.11233025079402381 1102 72-73 0.17465125537888326 1102 74-75 0.13722960290628095 1102 76-77 0.12054878649981049 1102 78-79 0.069478643414147 1102 80-81 0.046992035018462275 1102 82-83 -0.05945015236151718 1102 84-85 -0.0926932638586706 1102 86-87 -0.1069305430632177 1102 88-89 -0.10107804227484252 1102 90-91 -0.15248397334060826 1102 92-93 -0.3011623711226683 1102 94-95 -0.3793774152755631 1102 96-97 -0.44796411120777435 1102 98-99 -0.44007814795942224 1102 100-101 -0.39088064082331186 1103 1 0.2782544177102295 1103 2 0.24881508956100618 1103 3 0.30090450641395705 1103 4 0.2845868192047476 1103 5 0.3141020602122282 1103 6 0.31477104211447227 1103 7 0.30991718904397203 1103 8 0.2734630966408389 1103 9 0.36180116978002275 1103 10-11 0.388619214555483 1103 12-13 0.37523838541543597 1103 14-15 0.41533098375554545 1103 16-17 0.45255063116454863 1103 18-19 0.4382515613495599 1103 20-21 0.42326008594707076 1103 22-23 0.38050186624500526 1103 24-25 0.37694811298103303 1103 26-27 0.44162517540687674 1103 28-29 0.43247587831701395 1103 30-31 0.4523531765877351 1103 32-33 0.35353255566693065 1103 34-35 0.3434867009821687 1103 36-37 0.37937065010510196 1103 38-39 0.3756272068313251 1103 40-41 0.3424509188421254 1103 42-43 0.2793064334006843 1103 44-45 0.2447966076139565 1103 46-47 0.26434346727534574 1103 48-49 0.2814731578678007 1103 50-51 0.15980867338905824 1103 52-53 0.3317880227296257 1103 54-55 0.45168366114026526 1103 56-57 0.5105794871628078 1103 58-59 0.5277352890956379 1103 60-61 0.4689470580407047 1103 62-63 0.4642275941491647 1103 64-65 0.4250569582757251 1103 66-67 0.41309866955723606 1103 68-69 0.39545334587424463 1103 70-71 0.373488448032127 1103 72-73 0.43163230940346864 1103 74-75 0.37881354375102916 1103 76-77 0.3069243771607191 1103 78-79 0.31169500680422857 1103 80-81 0.3458750439613283 1103 82-83 0.3213963162439235 1103 84-85 0.264169053802636 1103 86-87 0.2378436244569393 1103 88-89 0.20859986055126356 1103 90-91 0.1346235496012511 1103 92-93 0.07323031398843938 1103 94-95 0.09646765006866076 1103 96-97 0.08224802973032652 1103 98-99 0.053679580940258376 1103 100-101 0.1051972976690223 1104 1 0.055951341416015765 1104 2 0.09643334597436848 1104 3 0.13401649139582972 1104 4 0.10389408528451582 1104 5 0.08763297643923096 1104 6 0.010896455605049482 1104 7 0.04210819709047797 1104 8 0.051575516875949745 1104 9 0.06787287680538867 1104 10-11 0.07303651927816901 1104 12-13 0.03885859479117215 1104 14-15 0.08438393420480139 1104 16-17 0.07046259362679308 1104 18-19 0.03731781067775586 1104 20-21 0.03284664267760462 1104 22-23 0.09375640139454333 1104 24-25 0.06652223187708017 1104 26-27 0.04655626819529601 1104 28-29 0.04974914922620144 1104 30-31 0.04817676561027184 1104 32-33 1.3307645689764058E-4 1104 34-35 -0.008142149116615371 1104 36-37 0.006172849269411529 1104 38-39 -0.025167556125978763 1104 40-41 -0.07894928137437063 1104 42-43 -0.1481842843508403 1104 44-45 -0.13516386949930137 1104 46-47 -0.15656640597134697 1104 48-49 -0.2819524459987619 1104 50-51 -0.33715598877872566 1104 52-53 -0.2909511867747909 1104 54-55 -0.16897579010002062 1104 56-57 -0.19737075866066078 1104 58-59 -0.08317430029023853 1104 60-61 -0.17652160088727697 1104 62-63 -0.14374083769018142 1104 64-65 -0.14444594467415328 1104 66-67 -0.1276148003951505 1104 68-69 -0.15217112235983166 1104 70-71 -0.20112609737424236 1104 72-73 -0.21345730072317792 1104 74-75 -0.1691428240038988 1104 76-77 -0.1351624255825108 1104 78-79 -0.12710467829951 1104 80-81 -0.1683227890452521 1104 82-83 -0.1798583816524797 1104 84-85 -0.17059141025301727 1104 86-87 -0.2333294350705657 1104 88-89 -0.29016802267975095 1104 90-91 -0.2829442505531681 1104 92-93 -0.3114853284453467 1104 94-95 -0.30917114083568364 1104 96-97 -0.2997613215909496 1104 98-99 -0.31648442437361624 1104 100-101 -0.3993005998685817 1105 1 0.254839770856524 1105 2 0.25510384373367145 1105 3 0.2897906972569757 1105 4 0.28983461145893585 1105 5 0.25600446165913127 1105 6 0.3046167976231331 1105 7 0.35165741932684114 1105 8 0.30380472506968204 1105 9 0.3662360035990062 1105 10-11 0.3408770323759853 1105 12-13 0.32075344237211567 1105 14-15 0.3857711001622661 1105 16-17 0.36883448233661653 1105 18-19 0.3731803189831666 1105 20-21 0.39448479409293924 1105 22-23 0.2923564238957326 1105 24-25 0.36623211673224176 1105 26-27 0.42777995299486093 1105 28-29 0.3716378001443914 1105 30-31 0.36648576879578343 1105 32-33 0.3467359269217667 1105 34-35 0.3719868851935786 1105 36-37 0.32872291441683643 1105 38-39 0.279453198688806 1105 40-41 0.32112776458460957 1105 42-43 0.32141819855532816 1105 44-45 0.3117527606471171 1105 46-47 0.33133338774811705 1105 48-49 0.2577103188909007 1105 50-51 0.17736486252021422 1105 52-53 0.21175101763496684 1105 54-55 0.3563001292679715 1105 56-57 0.38621431409711704 1105 58-59 0.43860612991837655 1105 60-61 0.41898880797464955 1105 62-63 0.40690870745592633 1105 64-65 0.38224476645768846 1105 66-67 0.34750761609126357 1105 68-69 0.38431357035166513 1105 70-71 0.4335532429992526 1105 72-73 0.39195996381542386 1105 74-75 0.31375207482320633 1105 76-77 0.3734112934989753 1105 78-79 0.3624050254858737 1105 80-81 0.3404004716481168 1105 82-83 0.3596002085007193 1105 84-85 0.3737907877715898 1105 86-87 0.3366347603528581 1105 88-89 0.3522259272097443 1105 90-91 0.3101085512572901 1105 92-93 0.3171195005927352 1105 94-95 0.31004304928641346 1105 96-97 0.2882016168288324 1105 98-99 0.2897376847536215 1105 100-101 0.26806289683987927 1106 1 0.26284637454026694 1106 2 0.2671584027680538 1106 3 0.31134184685156185 1106 4 0.30906438952877835 1106 5 0.3187413085349178 1106 6 0.3644901456839804 1106 7 0.41560334511255803 1106 8 0.4286882593882382 1106 9 0.4302806595514994 1106 10-11 0.46918627947339786 1106 12-13 0.4106882101124043 1106 14-15 0.48184680837209726 1106 16-17 0.48236476022953667 1106 18-19 0.4463141161131716 1106 20-21 0.45471991518096644 1106 22-23 0.49802778699333317 1106 24-25 0.46922035286532804 1106 26-27 0.48429898853387243 1106 28-29 0.5426498252676524 1106 30-31 0.5396643823494998 1106 32-33 0.5484067697652648 1106 34-35 0.5586148921408594 1106 36-37 0.5052895712937939 1106 38-39 0.48122208208745576 1106 40-41 0.4994061346693357 1106 42-43 0.4896025892955933 1106 44-45 0.5284794772116683 1106 46-47 0.5695392982583343 1106 48-49 0.48845683947172347 1106 50-51 0.4867176278683232 1106 52-53 0.5818852060570805 1106 54-55 0.6221692494635178 1106 56-57 0.7202259429297513 1106 58-59 0.7301813189108302 1106 60-61 0.6744130105688768 1106 62-63 0.6964363932978372 1106 64-65 0.7229140955250202 1106 66-67 0.7434682869024911 1106 68-69 0.7361377235752542 1106 70-71 0.7567195860945972 1106 72-73 0.7010986302143181 1106 74-75 0.7371518037771523 1106 76-77 0.7329322666892395 1106 78-79 0.6948851056148513 1106 80-81 0.6047598469003574 1106 82-83 0.6427380642495955 1106 84-85 0.6895626107114694 1106 86-87 0.6736678460850776 1106 88-89 0.7220241590714949 1106 90-91 0.6623970167376108 1106 92-93 0.6566577647990322 1106 94-95 0.736506157971867 1106 96-97 0.7726834811728018 1106 98-99 0.7315601379226031 1106 100-101 0.7397617593898644 1107 1 0.22167517931277203 1107 2 0.18590964748635486 1107 3 0.18158644466095097 1107 4 0.21530636831592886 1107 5 0.1949033709942185 1107 6 0.2152964317569399 1107 7 0.21913371385419111 1107 8 0.28031049520583196 1107 9 0.33451274525283026 1107 10-11 0.29792166199883496 1107 12-13 0.283129164997753 1107 14-15 0.37891107394501944 1107 16-17 0.31898436654400797 1107 18-19 0.2981792908180765 1107 20-21 0.3455331788937812 1107 22-23 0.3834984169491591 1107 24-25 0.3168353051695121 1107 26-27 0.3742473363723562 1107 28-29 0.359574741385309 1107 30-31 0.41148656100005354 1107 32-33 0.43438507309420515 1107 34-35 0.4624078785683068 1107 36-37 0.47097449044580486 1107 38-39 0.4816891229420861 1107 40-41 0.46192170841344904 1107 42-43 0.41545123759327396 1107 44-45 0.4614909607127231 1107 46-47 0.5023252811440315 1107 48-49 0.46069615503577666 1107 50-51 0.45712456657556544 1107 52-53 0.5201872191930477 1107 54-55 0.5907244185210132 1107 56-57 0.5709615883113557 1107 58-59 0.6297272388613138 1107 60-61 0.6691331411423604 1107 62-63 0.6733639770676305 1107 64-65 0.6694184217720078 1107 66-67 0.6305907739791436 1107 68-69 0.6572667820203719 1107 70-71 0.6658905893845528 1107 72-73 0.6383312334781834 1107 74-75 0.687978970051411 1107 76-77 0.644325192727365 1107 78-79 0.6364262710406621 1107 80-81 0.6466871682891693 1107 82-83 0.6212770698669665 1107 84-85 0.645047965293088 1107 86-87 0.6958565633151252 1107 88-89 0.7309507028926916 1107 90-91 0.7510442749333563 1107 92-93 0.7523709569934134 1107 94-95 0.7412350811103394 1107 96-97 0.7182827745543001 1107 98-99 0.8204709112699753 1107 100-101 0.8646170878546897 1108 1 0.1661723093650629 1108 2 0.1445071578026571 1108 3 0.12999159631561952 1108 4 0.17299682663396965 1108 5 0.19201411302361038 1108 6 0.2239853089062862 1108 7 0.18880077715846255 1108 8 0.20105436151659717 1108 9 0.2376701902704781 1108 10-11 0.22962655577311608 1108 12-13 0.2722699120429297 1108 14-15 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-0.5813800877036321 2316 100-101 -0.567004200537184 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 100257.0 3 21211.0 4 16816.0 5 14773.0 6 15391.0 7 16477.0 8 18942.0 9 22648.0 10 27397.0 11 32829.0 12 39113.0 13 47068.0 14 55020.0 15 65360.0 16 79460.0 17 99113.0 18 79645.0 19 48732.0 20 43926.0 21 44516.0 22 46758.0 23 50904.0 24 58158.0 25 69551.0 26 83799.0 27 101162.0 28 123279.0 29 151261.0 30 189046.0 31 239211.0 32 310238.0 33 415635.0 34 584861.0 35 879968.0 36 1375555.0 37 2076939.0 38 2153539.0 39 516498.0 40 201.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.147485774820694 20.686076088299178 15.63744878925753 21.528989347622602 2 15.454776191723592 20.6923065438421 40.60712574575128 23.245791518683024 3 17.959859499231154 26.434016922643046 31.248459134377445 24.35766444374836 4 12.412306291454613 17.834381072100367 39.28865256354249 30.46466007290253 5 12.597431228094939 38.051470445666915 35.46087588444288 13.890222441795263 6 29.062543021959375 38.832575500508995 18.007726986281448 14.097154491250183 7 25.830778264560646 34.03775255287943 22.73145326417529 17.40001591838464 8 25.29947447453998 35.25433650362648 20.417517748008958 19.028671273824582 9 25.371851924531803 16.93547456646564 22.104166143516473 35.58850736548609 10-11 23.632446452111548 27.377727712136913 28.716843025892096 20.272982809859442 12-13 24.70314526312197 25.230563303156472 28.898968849473505 21.167322584248048 14-15 22.03067333056129 26.81897013142846 27.31504914885657 23.83530738915368 16-17 20.42487566645861 30.15885065374362 27.987189801169098 21.429083878628667 18-19 20.47027931392939 28.275470433218587 31.184103660482933 20.070146592369092 20-21 21.63994338042809 27.19564435300622 31.141084467804237 20.023327798761454 22-23 21.278892099162523 27.154184036349648 31.28352412234164 20.283399742146184 24-25 20.965483214970064 27.444602641319577 31.025259722874686 20.56465442083567 26-27 20.616860163262192 27.608365016984038 31.070339376869434 20.704435442884336 28-29 20.654875661640208 27.472716301252927 31.271079674376974 20.601328362729898 30-31 20.940809302317014 27.761568408774885 30.790968681295215 20.506653607612886 32-33 20.602996490146026 27.608434100236433 31.15197169896971 20.63659771064783 34-35 21.065698063525655 27.530643179455904 30.835097864913152 20.568560892105285 36-37 21.404861428135035 27.389509978413233 30.501403910396753 20.704224683054974 38-39 21.000527093753178 27.757089566093356 30.594418661736395 20.647964678417065 40-41 20.84959428628516 27.542633301762713 30.756568814229762 20.85120359772236 42-43 20.978654277295316 27.682696717358525 30.367726209202438 20.97092279614372 44-45 20.960869011329173 27.94624988632435 30.08283343516474 21.010047667181738 46-47 21.536449746034513 27.989205339679263 29.551374017905907 20.922970896380317 48-49 21.468922692139465 27.971213090923687 29.349354563639995 21.21050965329685 50-51 21.400904131239315 28.22696507580042 28.989782787651702 21.382348005308565 52-53 21.38592303268445 28.309037209735926 28.997240062229128 21.307799695350496 54-55 21.3398389978434 28.700507523659162 28.91491174083291 21.04474173766452 56-57 21.385829979375025 28.351175868020313 28.937262995509894 21.325731157094772 58-59 21.386693854270945 28.0576783887195 29.10371506454818 21.451912692461377 60-61 21.558301743341016 28.176255070536104 29.04336673175728 21.2220764543656 62-63 21.280299292002624 28.278300263402556 29.15489660213611 21.28650384245871 64-65 21.423482100317678 28.272206261918626 28.977504268903886 21.32680736885981 66-67 21.234848937631522 28.18857053195714 28.98422291936268 21.592357611048655 68-69 21.440266213283277 28.56417148265336 28.734224184836943 21.26133811922642 70-71 21.589979454692592 28.17282488645791 28.665267572515095 21.571928086334403 72-73 21.32372775710451 28.317379163663592 28.848832046977257 21.510061032254644 74-75 21.380961590756073 28.46250083009415 28.75045261337874 21.40608496577104 76-77 21.463074167630815 28.58991728892383 28.514963814903993 21.43204472854137 78-79 21.49169464284892 28.496673760995055 28.76906979466682 21.242561801489206 80-81 21.41095172271234 28.550081593462984 28.652438219772293 21.386528464052386 82-83 21.309363781907088 28.450470132142737 28.91472174475571 21.325444341194466 84-85 21.227535392055625 28.490601141647627 28.70602345790459 21.57584000839216 86-87 21.39948138809758 28.548769398320324 28.594699218814952 21.457049994767154 88-89 21.278298707898006 28.6035885921353 28.669575425888468 21.448537274078227 90-91 21.164949027790495 28.415309631857383 28.76307073103898 21.656670609313142 92-93 21.301452591435645 28.55461350008352 28.60983324432187 21.534100664158963 94-95 21.321764619401645 28.28642944950856 28.86249160758632 21.529314323503474 96-97 21.286477593694272 28.524609394124028 28.64852523954042 21.540387772641278 98-99 21.165364309713976 28.476625076463453 28.591060304655496 21.766950309167076 100-101 21.267878165605133 28.490231680811164 28.421544039423768 21.820346114159936 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 6581.0 1 4200.0 2 2342.0 3 2952.0 4 2561.5 5 2438.0 6 4162.0 7 8111.0 8 13413.0 9 16389.5 10 14423.0 11 11789.5 12 11672.5 13 12358.5 14 13159.0 15 14002.0 16 14776.0 17 15855.5 18 17097.5 19 18833.0 20 21404.0 21 25054.0 22 31814.5 23 43161.5 24 55324.0 25 67469.5 26 85374.5 27 109860.5 28 143278.0 29 172962.0 30 189687.5 31 212626.0 32 243852.5 33 270261.0 34 290870.5 35 311388.5 36 325695.5 37 334905.0 38 349594.0 39 359675.5 40 367826.0 41 368485.0 42 360155.0 43 356677.0 44 354434.5 45 348409.0 46 340920.5 47 333026.0 48 323515.5 49 317478.0 50 311989.5 51 295686.0 52 270106.0 53 247018.5 54 233418.5 55 226016.5 56 208076.5 57 182834.5 58 161533.5 59 143004.5 60 124044.5 61 105948.0 62 89803.5 63 75550.5 64 63174.5 65 53926.5 66 45864.5 67 37686.5 68 29291.0 69 21339.5 70 16664.0 71 12553.5 72 9415.5 73 7252.5 74 5487.0 75 4130.5 76 3058.0 77 2272.0 78 1865.0 79 1603.5 80 1199.0 81 786.0 82 599.0 83 484.0 84 417.0 85 346.0 86 296.0 87 250.0 88 218.0 89 202.0 90 182.5 91 146.0 92 110.5 93 89.0 94 76.0 95 69.5 96 57.5 97 42.5 98 26.0 99 19.5 100 12.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014570650057482815 2 0.004759939573003368 3 0.004246137541701578 4 0.006427372580246909 5 0.0028016752272871145 6 0.0073386441074614036 7 0.0013572129128726507 8 0.004246137541701578 9 0.011032202106064832 10-11 0.007736113603374108 12-13 0.009238742185483114 14-15 0.0035675310852652533 16-17 0.006427372580246909 18-19 0.00416373532913431 20-21 9.306602831126748E-4 22-23 0.0010663815743999399 24-25 0.007304713784639587 26-27 0.0012748107003053826 28-29 7.51314291054503E-4 30-31 6.15592999767238E-4 32-33 0.0015074757710835513 34-35 0.0019049452669962562 36-37 0.0013572129128726507 38-39 0.0019776531016144337 40-41 0.002777439282414389 42-43 0.002777439282414389 44-45 0.007978473052101368 46-47 0.014095625537977385 48-49 0.005511253864057871 50-51 0.005855404281250579 52-53 0.013935668301817396 54-55 0.008346859414166801 56-57 0.00561304483252332 58-59 0.007295019406690497 60-61 0.001449309503389009 62-63 0.0023945113534253196 64-65 0.0024769135659925877 66-67 0.00254477421163622 68-69 0.0021279159598253344 70-71 0.0024187472982980453 72-73 0.0013329769679999246 74-75 0.0015704892277526386 76-77 0.008322623469294075 78-79 0.006587329816406901 80-81 0.012777190136901097 82-83 0.015132923978530055 84-85 0.008395331303912254 86-87 0.005889334604072395 88-89 0.003107048132683461 90-91 0.0026514123690762137 92-93 0.0010276040626035785 94-95 0.0013329769679999246 96-97 0.0018613205662253497 98-99 0.006674579217948714 100-101 0.01138604690120663 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.0315257E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.191490020108326 #Duplication Level Percentage of deduplicated Percentage of total 1 66.70027588838158 27.47483748594742 2 16.802714792671136 13.842577173860793 3 6.657729365897144 8.227253781958032 4 3.173929407810361 5.229555261053951 5 1.8419069016267873 3.7935444878164226 6 1.1067307057244817 2.735273209187846 7 0.7127813690311575 2.0552368654276254 8 0.4847165304001519 1.597295689964751 9 0.35796947433667625 1.327076642667836 >10 1.8873934834512465 14.149055117155001 >50 0.15189786997168853 4.370348094158252 >100 0.10867218107633088 8.645490401796646 >500 0.00907778505328728 2.567578819765205 >1k 0.0040625286755463464 2.7755244215889086 >5k 4.7238630787943184E-5 0.11150576457845604 >10k+ 9.447726157588637E-5 1.0978467830728174 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 44902 0.43529695867005547 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 37020 0.3588858716753252 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20573 0.19944243754663601 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10346 0.10029803426128889 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.9388755898180723E-5 0.0 5 0.0 0.0 0.0 1.9388755898180723E-5 0.0 6 0.0 9.694377949090362E-6 0.0 1.9388755898180723E-5 0.0 7 0.0 9.694377949090362E-6 0.0 1.9388755898180723E-5 0.0 8 0.0 9.694377949090362E-6 0.0 1.9388755898180723E-5 0.0 9 0.0 9.694377949090362E-6 0.0 1.9388755898180723E-5 0.0 10-11 0.0 4.362470077090663E-5 0.0 2.9083133847271085E-5 0.0 12-13 0.0 7.75550235927229E-5 0.0 3.3930322821816264E-5 0.0 14-15 0.0 7.75550235927229E-5 0.0 3.877751179636145E-5 0.0 16-17 0.0 8.724940154181326E-5 0.0 5.331907871999699E-5 0.0 18-19 4.847188974545181E-6 9.694377949090362E-5 0.0 5.816626769454217E-5 0.0 20-21 9.694377949090362E-6 1.0663815743999398E-4 0.0 8.240221256726807E-5 0.0 22-23 9.694377949090362E-6 1.1633253538908434E-4 0.0 1.744988030836265E-4 0.0 24-25 9.694377949090362E-6 1.1633253538908434E-4 0.0 3.344560392436175E-4 0.0 26-27 9.694377949090362E-6 1.1633253538908434E-4 0.0 6.786064564363254E-4 0.0 28-29 9.694377949090362E-6 1.1633253538908434E-4 0.0 0.0016480442513453616 0.0 30-31 9.694377949090362E-6 1.1633253538908434E-4 0.0 0.004100721872465223 4.847188974545181E-6 32-33 9.694377949090362E-6 1.1633253538908434E-4 0.0 0.009195117484712209 9.694377949090362E-6 34-35 9.694377949090362E-6 1.260269133381747E-4 0.0 0.01718813210373721 9.694377949090362E-6 36-37 9.694377949090362E-6 1.3572129128726506E-4 0.0 0.03034340298065283 9.694377949090362E-6 38-39 9.694377949090362E-6 1.4541566923635544E-4 0.0 0.04902931647752451 9.694377949090362E-6 40-41 9.694377949090362E-6 1.4541566923635544E-4 0.0 0.06884947219443975 9.694377949090362E-6 42-43 9.694377949090362E-6 1.4541566923635544E-4 0.0 0.08893137611598044 9.694377949090362E-6 44-45 9.694377949090362E-6 1.502628582109006E-4 0.0 0.11406889813797175 9.694377949090362E-6 46-47 9.694377949090362E-6 1.551100471854458E-4 0.0 0.14573073651970087 9.694377949090362E-6 48-49 9.694377949090362E-6 1.551100471854458E-4 0.0 0.1783038464286445 9.694377949090362E-6 50-51 9.694377949090362E-6 1.551100471854458E-4 0.0 0.2129709419745916 9.694377949090362E-6 52-53 9.694377949090362E-6 1.551100471854458E-4 0.0 0.24692065355230605 9.694377949090362E-6 54-55 9.694377949090362E-6 1.6480442513453617E-4 0.0 0.28625074489176566 9.694377949090362E-6 56-57 9.694377949090362E-6 1.8419318103271687E-4 0.0 0.33410219444847566 9.694377949090362E-6 58-59 9.694377949090362E-6 1.8419318103271687E-4 0.0 0.38820167059337446 9.694377949090362E-6 60-61 9.694377949090362E-6 1.8419318103271687E-4 0.0 0.44633885515406935 9.694377949090362E-6 62-63 9.694377949090362E-6 1.8419318103271687E-4 0.0 0.5070886745720441 9.694377949090362E-6 64-65 1.4541566923635543E-5 1.8419318103271687E-4 0.0 0.5712509150280987 9.694377949090362E-6 66-67 1.9388755898180723E-5 1.987347479563524E-4 0.0 0.6488931880223634 9.694377949090362E-6 68-69 1.9388755898180723E-5 2.1327631487998795E-4 0.0 0.7390993748386492 9.694377949090362E-6 70-71 1.9388755898180723E-5 2.1327631487998795E-4 0.0 0.8392374518637781 9.694377949090362E-6 72-73 1.9388755898180723E-5 2.1327631487998795E-4 0.0 0.9454635982409357 9.694377949090362E-6 74-75 1.9388755898180723E-5 2.1327631487998795E-4 4.847188974545181E-6 1.056168547230573 9.694377949090362E-6 76-77 1.9388755898180723E-5 2.1327631487998795E-4 9.694377949090362E-6 1.177343424405228 1.4541566923635543E-5 78-79 1.9388755898180723E-5 2.1812350385453315E-4 9.694377949090362E-6 1.3217848086577 1.9388755898180723E-5 80-81 1.9388755898180723E-5 2.2297069282907833E-4 9.694377949090362E-6 1.485353200603727 1.9388755898180723E-5 82-83 1.9388755898180723E-5 2.326650707781687E-4 9.694377949090362E-6 1.6573460069875137 1.9388755898180723E-5 84-85 1.9388755898180723E-5 2.520538266763494E-4 9.694377949090362E-6 1.8313213136618893 1.9388755898180723E-5 86-87 1.9388755898180723E-5 2.520538266763494E-4 9.694377949090362E-6 2.020943346346097 2.9083133847271085E-5 88-89 1.9388755898180723E-5 2.5690101565089456E-4 9.694377949090362E-6 2.2377387204216044 2.9083133847271085E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 24185 0.0 54.248707 1 GTATCAA 46100 0.0 43.359737 1 ATCAACG 57260 0.0 34.592487 3 TCAACGC 57850 0.0 34.240353 4 TATCAAC 59405 0.0 33.422085 2 CAACGCA 60060 0.0 32.99576 5 AACGCAG 63885 0.0 31.10244 6 CGCAGAG 69165 0.0 28.228125 8 ACGCAGA 70300 0.0 28.002125 7 TGGTATC 10805 0.0 27.870499 2 GTGGTAT 11285 0.0 27.318382 1 GCAGAGT 74065 0.0 25.44985 9 GTACATG 40960 0.0 23.866966 1 TACATGG 39850 0.0 23.290375 2 GAGTACT 47495 0.0 22.26414 12-13 ACATGGG 41040 0.0 21.956194 3 AGAGTAC 66405 0.0 21.732956 10-11 GTACTTT 48490 0.0 21.589565 14-15 CAGAGTA 71640 0.0 21.50088 10-11 AGTACTT 48195 0.0 20.521421 12-13 >>END_MODULE