FastQCFastQC Report
Wed 25 May 2016
SRR2049415_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049415_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10315257
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT437100.42374126015473973No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT367980.3567337197706271No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243450.23600963117060483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA232650.054.7451741
GTATCAA428850.041.2463041
ATCAACG559000.031.4045123
TCAACGC565800.030.9932024
TATCAAC577000.030.4830362
CAACGCA583250.030.0820715
AACGCAG628250.028.085726
TGGTATC102750.026.2571052
GTGGTAT109650.025.3548661
CGCAGAG701200.024.7706938
ACGCAGA714800.024.5319587
GCAGAGT759200.022.4593669
GTACATG392800.022.1166211
CGATACA26950.021.854622
TACATGG381800.021.3108982
AGAGTAC679050.021.15490710-11
CTGGTCG29850.021.0033669
GAGTACT484050.020.95408212-13
AGTACTT492500.020.7247612-13
TCGATAC28400.020.415291
Produced by FastQC (version 0.11.5)