##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049415_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10315257 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06270837459503 33.0 31.0 34.0 30.0 34.0 2 32.25017030598462 34.0 31.0 34.0 30.0 34.0 3 32.316684305587344 34.0 31.0 34.0 30.0 34.0 4 35.95715928357384 37.0 35.0 37.0 35.0 37.0 5 35.844111203433904 37.0 35.0 37.0 35.0 37.0 6 35.87436852033837 37.0 35.0 37.0 35.0 37.0 7 35.798169255501826 37.0 35.0 37.0 35.0 37.0 8 35.792994978215276 37.0 35.0 37.0 35.0 37.0 9 37.39695792358833 39.0 37.0 39.0 34.0 39.0 10-11 37.39211277043316 39.0 37.0 39.0 34.0 39.0 12-13 37.371784629311705 39.0 37.0 39.0 34.0 39.0 14-15 38.58639828362978 40.0 38.0 41.0 34.0 41.0 16-17 38.577086009587546 40.0 38.0 41.0 34.0 41.0 18-19 38.62617324997332 40.0 38.0 41.0 34.0 41.0 20-21 38.62232254610816 40.0 38.0 41.0 34.0 41.0 22-23 38.54032764283042 40.0 38.0 41.0 34.0 41.0 24-25 38.444416363062984 40.0 38.0 41.0 34.0 41.0 26-27 38.26093455548417 40.0 38.0 41.0 34.0 41.0 28-29 38.07446527992468 40.0 38.0 41.0 33.0 41.0 30-31 37.87441534418386 40.0 37.0 41.0 33.0 41.0 32-33 37.61573327741616 40.0 37.0 41.0 33.0 41.0 34-35 37.42339298962692 40.0 36.0 41.0 32.5 41.0 36-37 37.21971076435614 39.0 36.0 41.0 32.0 41.0 38-39 37.354609148371196 40.0 37.0 41.0 32.5 41.0 40-41 37.24807632034762 40.0 36.5 41.0 32.0 41.0 42-43 37.04732737148478 40.0 36.0 41.0 31.0 41.0 44-45 36.79180223042431 40.0 36.0 41.0 31.0 41.0 46-47 36.437919675680405 39.5 36.0 41.0 30.0 41.0 48-49 36.137347426244446 39.0 35.0 41.0 29.5 41.0 50-51 35.89761234257178 39.0 35.0 41.0 29.0 41.0 52-53 35.71171469600806 39.0 35.0 40.0 28.0 41.0 54-55 35.602873878954256 39.0 35.0 40.0 28.0 41.0 56-57 35.50224274586664 39.0 35.0 40.0 28.0 41.0 58-59 35.25787820894816 38.5 35.0 40.0 27.5 41.0 60-61 35.01243415457317 38.0 34.5 40.0 27.0 41.0 62-63 34.73720533574685 38.0 34.0 40.0 26.0 41.0 64-65 34.47990132480461 37.0 34.0 40.0 26.0 41.0 66-67 34.139966362447396 37.0 34.0 40.0 26.0 41.0 68-69 33.821647100018936 36.0 34.0 39.0 26.0 41.0 70-71 33.49151882497935 36.0 33.0 39.0 25.0 41.0 72-73 33.124394283147765 35.0 33.0 39.0 24.5 40.0 74-75 32.75017457151092 35.0 33.0 38.0 23.5 40.0 76-77 30.74147997475972 33.5 30.0 35.5 21.0 38.5 78-79 32.05949013194727 35.0 32.0 37.0 23.5 39.0 80-81 32.07705358189331 35.0 33.0 36.5 23.5 39.0 82-83 31.80953804641028 35.0 32.5 36.0 23.0 38.0 84-85 31.5501591477556 35.0 32.0 36.0 22.5 37.0 86-87 31.293218530570783 35.0 32.0 35.0 21.0 37.0 88-89 31.032143939797137 35.0 32.0 35.0 20.0 36.5 90-91 30.771201241035484 35.0 32.0 35.0 18.5 36.0 92-93 30.535618162494643 34.5 32.0 35.0 17.5 36.0 94-95 30.276796932931482 34.0 31.0 35.0 12.5 36.0 96-97 30.04004059229935 34.0 31.0 35.0 4.5 35.0 98-99 29.75442075752451 34.0 31.0 35.0 2.0 35.0 100-101 29.146600128334175 34.0 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3848930189997617 1101 2 0.4028303770103321 1101 3 0.34072551639660986 1101 4 0.26540348785827206 1101 5 0.29559606038269237 1101 6 0.290861087080728 1101 7 0.321798326416598 1101 8 0.3164627072200972 1101 9 0.3810125113026501 1101 10-11 0.4160586816362368 1101 12-13 0.3564796931406349 1101 14-15 0.466165680892054 1101 16-17 0.44276583959597104 1101 18-19 0.4050676325970173 1101 20-21 0.3488162401488779 1101 22-23 0.278966150254206 1101 24-25 0.2795272725587026 1101 26-27 0.2863040378803632 1101 28-29 0.3035461615372981 1101 30-31 0.24504495775909163 1101 32-33 0.24501750470334827 1101 34-35 0.1994444738907717 1101 36-37 0.15869584689999527 1101 38-39 0.26268546979980556 1101 40-41 0.2479459468443963 1101 42-43 0.2530029335970383 1101 44-45 0.21502867721910235 1101 46-47 0.23050531991300716 1101 48-49 0.12276727078412364 1101 50-51 0.11417814960924488 1101 52-53 0.09326894718454781 1101 54-55 0.16646994133055415 1101 56-57 0.04462415158318578 1101 58-59 0.056274797532744 1101 60-61 0.03298065562956509 1101 62-63 0.027177515728624257 1101 64-65 -0.05559587783303499 1101 66-67 -0.06404128068155757 1101 68-69 -0.11681697910504596 1101 70-71 -0.03883824183230189 1101 72-73 -0.10314044555698842 1101 74-75 -0.09171976729497544 1101 76-77 -0.13069424011255037 1101 78-79 -0.10224254034674374 1101 80-81 -0.04601920534053505 1101 82-83 -0.08362203143891378 1101 84-85 -0.18370445195017382 1101 86-87 -0.18879151903380986 1101 88-89 -0.300412905531914 1101 90-91 -0.23517591272150185 1101 92-93 -0.2694830388159666 1101 94-95 -0.23655170683646531 1101 96-97 -0.3722972362292509 1101 98-99 -0.45746952577681554 1101 100-101 -0.5509180691491693 1102 1 0.3409138490947896 1102 2 0.31883872499743404 1102 3 0.3298445982347289 1102 4 0.2901110433020264 1102 5 0.25783484743494967 1102 6 0.28817340608327413 1102 7 0.27707089458153433 1102 8 0.23039759246687908 1102 9 0.38605863966915877 1102 10-11 0.30227545862013727 1102 12-13 0.2727144323008801 1102 14-15 0.3638440631162112 1102 16-17 0.3552802995369291 1102 18-19 0.2757081538858941 1102 20-21 0.3175401192593128 1102 22-23 0.309801199384367 1102 24-25 0.2780440801279056 1102 26-27 0.25091318549389285 1102 28-29 0.33692358313675186 1102 30-31 0.27109378061413736 1102 32-33 0.27828130524506633 1102 34-35 0.21569418963552067 1102 36-37 0.22643938375009043 1102 38-39 0.38138549821317724 1102 40-41 0.41351135910325354 1102 42-43 0.41065069243382624 1102 44-45 0.407505423397275 1102 46-47 0.4422287398387681 1102 48-49 0.2542873933003591 1102 50-51 0.28966862869948784 1102 52-53 0.3538568372868909 1102 54-55 0.30141792282247337 1102 56-57 0.23059858425185098 1102 58-59 0.18914860716576243 1102 60-61 0.27682384495481926 1102 62-63 0.30361374019651066 1102 64-65 0.2503294481710654 1102 66-67 0.20466441662838264 1102 68-69 0.21108227046109818 1102 70-71 0.07321228031399585 1102 72-73 0.015661594629847286 1102 74-75 0.02935246538798708 1102 76-77 0.03516531453504612 1102 78-79 0.1446750427307606 1102 80-81 0.12312553362360035 1102 82-83 0.17716216294807907 1102 84-85 0.2195504793415033 1102 86-87 0.11509480739316658 1102 88-89 0.08202657909852817 1102 90-91 0.09901449510029536 1102 92-93 0.06691744850653336 1102 94-95 1.8767433560640256E-4 1102 96-97 -0.044611330054312504 1102 98-99 -0.05370850668182925 1102 100-101 -0.026112716122383972 1103 1 0.33162463013633925 1103 2 0.35583099049445366 1103 3 0.28527116528284324 1103 4 0.26026529484244776 1103 5 0.27718093745220784 1103 6 0.2844040125747256 1103 7 0.2532070927765915 1103 8 0.2883753956029693 1103 9 0.3395450811448839 1103 10-11 0.3304018807620466 1103 12-13 0.3144102978450718 1103 14-15 0.39637961483662565 1103 16-17 0.41742359102254767 1103 18-19 0.3521586241245771 1103 20-21 0.3443254816856296 1103 22-23 0.3090989889556113 1103 24-25 0.3390737815158644 1103 26-27 0.3266380088924947 1103 28-29 0.31961710893774864 1103 30-31 0.3320709766448644 1103 32-33 0.36988572493320504 1103 34-35 0.3024957967594091 1103 36-37 0.3489772839840626 1103 38-39 0.3909049738222592 1103 40-41 0.4329254244581904 1103 42-43 0.49357187300528693 1103 44-45 0.40781497892402996 1103 46-47 0.518068927875774 1103 48-49 0.4363162118031312 1103 50-51 0.422283055394189 1103 52-53 0.37743602068631077 1103 54-55 0.33056796555373325 1103 56-57 0.3250791291069959 1103 58-59 0.2994754744533168 1103 60-61 0.3319658813012367 1103 62-63 0.31605777297434656 1103 64-65 0.2970144871651641 1103 66-67 0.23478175947530389 1103 68-69 0.2264644301385843 1103 70-71 0.2220660059238 1103 72-73 0.26185448847606096 1103 74-75 0.24995626237896573 1103 76-77 0.2624682854534335 1103 78-79 0.3245426586776503 1103 80-81 0.3095427689846133 1103 82-83 0.38304229033300885 1103 84-85 0.34068056750333753 1103 86-87 0.3451387664997938 1103 88-89 0.26482586109653994 1103 90-91 0.3945965512872647 1103 92-93 0.33382568886569075 1103 94-95 0.30143508585462797 1103 96-97 0.26367713465123543 1103 98-99 0.23056892585687194 1103 100-101 0.21676686847331794 1104 1 0.3037264775024937 1104 2 0.25641488271818247 1104 3 0.26821175510775674 1104 4 0.21798790795151746 1104 5 0.20910913769898087 1104 6 0.25124867258517014 1104 7 0.2678401932179 1104 8 0.2517013170151614 1104 9 0.33158404387963714 1104 10-11 0.29978342375370914 1104 12-13 0.21482234073454975 1104 14-15 0.31238284517263537 1104 16-17 0.34185521652307926 1104 18-19 0.3393623964454946 1104 20-21 0.36333173230065796 1104 22-23 0.3192042871230001 1104 24-25 0.26131778164455 1104 26-27 0.2679258948485739 1104 28-29 0.2987754095955495 1104 30-31 0.2907410650958937 1104 32-33 0.30401893229542054 1104 34-35 0.21049375485398514 1104 36-37 0.21688425049605797 1104 38-39 0.2782073073473583 1104 40-41 0.3038221265633041 1104 42-43 0.30296251846692 1104 44-45 0.3572250357340394 1104 46-47 0.4452296432428682 1104 48-49 0.3849179938813023 1104 50-51 0.4236359785928059 1104 52-53 0.34567801865008363 1104 54-55 0.3580183987253278 1104 56-57 0.3798939383146873 1104 58-59 0.3290160788497474 1104 60-61 0.35061084333958803 1104 62-63 0.33293263104394555 1104 64-65 0.3281518417338063 1104 66-67 0.36299296327222663 1104 68-69 0.30977378827251556 1104 70-71 0.24662979228637738 1104 72-73 0.24977588457922195 1104 74-75 0.27202852198670513 1104 76-77 0.19026321210379749 1104 78-79 0.33002450294025465 1104 80-81 0.3628518763748332 1104 82-83 0.30023782122842135 1104 84-85 0.2237951829287148 1104 86-87 0.2526275918459149 1104 88-89 0.21457419483358464 1104 90-91 0.2069523121477772 1104 92-93 0.18737050481034245 1104 94-95 0.14527308743667433 1104 96-97 0.10287588547442894 1104 98-99 0.12489927928250566 1104 100-101 0.1613126107504108 1105 1 0.2795631742335729 1105 2 0.29083095328002884 1105 3 0.24877355444898086 1105 4 0.21540365807756956 1105 5 0.2141364159739041 1105 6 0.1957116716756957 1105 7 0.2210097390944128 1105 8 0.23966730369822642 1105 9 0.24276604551950953 1105 10-11 0.2289317713888508 1105 12-13 0.23832916295393858 1105 14-15 0.31921066041312685 1105 16-17 0.27831792345156714 1105 18-19 0.25321538356680406 1105 20-21 0.27079411643720874 1105 22-23 0.17731153356639595 1105 24-25 0.21675592752294648 1105 26-27 0.16697021276800683 1105 28-29 0.20762799322693581 1105 30-31 0.28628257507887866 1105 32-33 0.3066265227360674 1105 34-35 0.3531866407081594 1105 36-37 0.25138654125856164 1105 38-39 0.300925041701376 1105 40-41 0.36096506154311214 1105 42-43 0.41635879622820227 1105 44-45 0.4010251185525604 1105 46-47 0.3876004587284001 1105 48-49 0.3975897127130281 1105 50-51 0.45029039035751595 1105 52-53 0.4152808402521728 1105 54-55 0.41054549734415247 1105 56-57 0.4483984279500035 1105 58-59 0.4761865984034017 1105 60-61 0.48334886705183777 1105 62-63 0.5131479444584244 1105 64-65 0.5002346979476684 1105 66-67 0.46157217806317163 1105 68-69 0.432974061231981 1105 70-71 0.3804361071098299 1105 72-73 0.44352857942067203 1105 74-75 0.438492707396513 1105 76-77 0.49327405685265546 1105 78-79 0.5005380189691166 1105 80-81 0.4846432479342724 1105 82-83 0.5177057835395189 1105 84-85 0.5315880459282774 1105 86-87 0.5235101097172787 1105 88-89 0.5375389415950629 1105 90-91 0.5613404564946762 1105 92-93 0.5256006448596331 1105 94-95 0.5520449000429934 1105 96-97 0.5290028446094404 1105 98-99 0.4962911369147669 1105 100-101 0.5828480312752689 1106 1 0.2908298913446359 1106 2 0.2631852291830228 1106 3 0.23097432706575916 1106 4 0.19101338211592633 1106 5 0.20198048866122775 1106 6 0.2234176002546704 1106 7 0.22215102316640412 1106 8 0.23829129249619285 1106 9 0.31424426104977954 1106 10-11 0.23843270326239718 1106 12-13 0.2297585402826101 1106 14-15 0.2820120891847111 1106 16-17 0.3046471512168907 1106 18-19 0.27439719754783454 1106 20-21 0.26691114487875467 1106 22-23 0.237288425190485 1106 24-25 0.28418941823212407 1106 26-27 0.22643514413859833 1106 28-29 0.3616515566909726 1106 30-31 0.30910079112602773 1106 32-33 0.30732798270586414 1106 34-35 0.3177612136599208 1106 36-37 0.31257586728529674 1106 38-39 0.3431044184005856 1106 40-41 0.32034401269365276 1106 42-43 0.34424718400549636 1106 44-45 0.3734991508340073 1106 46-47 0.4646972547613899 1106 48-49 0.38457239570466584 1106 50-51 0.4538055212322831 1106 52-53 0.4849344082600524 1106 54-55 0.43616442052368143 1106 56-57 0.3858225938143107 1106 58-59 0.3921522447855281 1106 60-61 0.44738160892241297 1106 62-63 0.4227990256065084 1106 64-65 0.3808875600154238 1106 66-67 0.4105345180862301 1106 68-69 0.4185950667608651 1106 70-71 0.44450476524893645 1106 72-73 0.4105610716240875 1106 74-75 0.4000383765689577 1106 76-77 0.3762838771811552 1106 78-79 0.4148408034893478 1106 80-81 0.47300684346145516 1106 82-83 0.46108581477919586 1106 84-85 0.42916546256062205 1106 86-87 0.4117870539258668 1106 88-89 0.48723024737177667 1106 90-91 0.4839353577896901 1106 92-93 0.46640016797052297 1106 94-95 0.4983089749696603 1106 96-97 0.46381820975591737 1106 98-99 0.5415682304497267 1106 100-101 0.6778913677647083 1107 1 0.18202499941892825 1107 2 0.1859317299382397 1107 3 0.16027664977813316 1107 4 0.14478358178754291 1107 5 0.12242686789813462 1107 6 0.1628927294281084 1107 7 0.14188284567535447 1107 8 0.15072363368210517 1107 9 0.22278477442766587 1107 10-11 0.19588213624107453 1107 12-13 0.17141772111902043 1107 14-15 0.1792277135787188 1107 16-17 0.2157207999787758 1107 18-19 0.19645756730322006 1107 20-21 0.16912273108001585 1107 22-23 0.17587063863186359 1107 24-25 0.20130034467587166 1107 26-27 0.2174181544112841 1107 28-29 0.27605325258399205 1107 30-31 0.2285360330093411 1107 32-33 0.24947074609959685 1107 34-35 0.2519679898388745 1107 36-37 0.27194586815524247 1107 38-39 0.3105573183229069 1107 40-41 0.3460095806535577 1107 42-43 0.3272531441916655 1107 44-45 0.25745448909743374 1107 46-47 0.2959246963505535 1107 48-49 0.22027826346620571 1107 50-51 0.2865683780106991 1107 52-53 0.2455084137713186 1107 54-55 0.2885286749854217 1107 56-57 0.30814593181408156 1107 58-59 0.40778263863019504 1107 60-61 0.41692648732838933 1107 62-63 0.40331005322451574 1107 64-65 0.4136060034428155 1107 66-67 0.41961291384160404 1107 68-69 0.42194507535582915 1107 70-71 0.3536530987853581 1107 72-73 0.3328446871231563 1107 74-75 0.35565437025625357 1107 76-77 0.3780209173465856 1107 78-79 0.46619128737360427 1107 80-81 0.500542677294753 1107 82-83 0.5458357626459431 1107 84-85 0.4877076556610014 1107 86-87 0.5014746865985025 1107 88-89 0.48287993197978096 1107 90-91 0.5572611216279171 1107 92-93 0.5979991093051389 1107 94-95 0.6977229217194179 1107 96-97 0.7165857464465155 1107 98-99 0.7605319773223691 1107 100-101 0.8790921187562155 1108 1 0.12144692290318915 1108 2 0.1346211481789652 1108 3 0.07900297250540689 1108 4 0.09515918826992475 1108 5 0.07968686899344846 1108 6 0.09086992190422905 1108 7 0.1296131439091326 1108 8 0.11887366293623103 1108 9 0.16642438237783352 1108 10-11 0.10258318690284085 1108 12-13 0.06873912346021172 1108 14-15 0.12692133174250841 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-0.869754870999671 2316 100-101 -1.1220892164118084 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 33.0 4 85.0 5 174.0 6 485.0 7 1293.0 8 3425.0 9 7319.0 10 12009.0 11 18454.0 12 25862.0 13 36895.0 14 47634.0 15 60462.0 16 74938.0 17 98058.0 18 82153.0 19 48098.0 20 37467.0 21 36435.0 22 37942.0 23 41361.0 24 49078.0 25 60460.0 26 76609.0 27 97495.0 28 125014.0 29 158026.0 30 199363.0 31 253932.0 32 326049.0 33 432418.0 34 602350.0 35 903190.0 36 1416838.0 37 2185555.0 38 2257594.0 39 500547.0 40 150.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.22545042305635 22.305148393196724 16.924184289892487 22.545216893854438 2 14.425358138077538 21.78345627384388 39.51672009040025 24.274465497678335 3 16.66075146891923 26.862000359274347 31.90755578374011 24.569692388066315 4 12.04798533431376 18.782015145558972 38.607283304742936 30.562716215384334 5 12.387165437177792 38.24211771003969 35.6235793128661 13.747137539916418 6 29.201621588450532 39.31690824037954 17.592433440351503 13.88903673081843 7 25.67136233251387 33.970428463391656 22.619533376628425 17.738675827466054 8 25.409529341782694 35.515027450603256 20.14701331697439 18.92842989063965 9 25.036976816939045 16.68046339559295 22.011057999871454 36.271501787596556 10-11 23.536479537516062 27.57826987416841 28.68737958066089 20.197871007654634 12-13 24.53248619981063 25.292937442082152 28.957848553846016 21.216727804261204 14-15 21.739106620197372 26.966499966045248 27.310536001803555 23.983857411953828 16-17 20.247585687879614 30.228413116609698 28.070701486157834 21.453299709352855 18-19 20.335046637985187 28.2678011551925 31.34360624591715 20.05354596090517 20-21 21.458162620577433 27.21797889184488 31.278076568018626 20.045781919559058 22-23 21.014507927432152 27.184771062902264 31.452527067430314 20.348193942235273 24-25 20.875601316137264 27.40272096900245 31.173758339101127 20.547919375759164 26-27 20.549768885171563 27.552301203509266 31.190028679635866 20.7079012316833 28-29 20.535069385263647 27.455800306327887 31.480241579812684 20.528888728595778 30-31 20.837402394044908 27.68684641255111 31.02116971501056 20.454581478393425 32-33 20.51625293505661 27.531987136213676 31.29748819554382 20.65427173318589 34-35 20.917662045332946 27.519458402446023 31.006359135011046 20.55652041720998 36-37 21.273508140259125 27.373950792706932 30.667980707048542 20.684560359985404 38-39 20.891469666731233 27.69137268155104 30.80691010683687 20.610247544880853 40-41 20.64055357662037 27.51634483696656 30.944259886373583 20.898841700039487 42-43 20.780242921353807 27.615168934731464 30.614075974774092 20.990512169140644 44-45 20.80849653389168 27.89959565381902 30.30071920281302 20.991188609476275 46-47 21.288803618778104 28.000717776092348 29.737902663880938 20.97257594124861 48-49 21.241683981203355 27.926583262175047 29.5485462381503 21.283186518471293 50-51 21.221244938365583 28.224877575072732 29.11745279758397 21.436424688977716 52-53 21.220601785639065 28.267099084698682 29.10900256746995 21.403296562192303 54-55 21.21097315363045 28.662393027747957 29.0092509336972 21.117382884924396 56-57 21.31418062421951 28.29985380360028 29.004413714919785 21.38155185726043 58-59 21.26977136202867 28.03797164022083 29.19197081735525 21.500286180395246 60-61 21.421566443699405 28.242319971213604 29.08933347688399 21.246780108203005 62-63 21.16363383950093 28.236460954828434 29.218873591693928 21.381031613976706 64-65 21.219756354299342 28.340487217723226 29.036822760171404 21.402933667806025 66-67 21.093424500516328 28.25311583935764 29.07383959157614 21.579620068549897 68-69 21.293561375426947 28.528712575634035 28.79385465273543 21.383871396203585 70-71 21.413475843175124 28.18457731900395 28.728582067048386 21.673364770772537 72-73 21.13127284554485 28.342876697919912 28.876337286885995 21.64951316964924 74-75 21.192770100848403 28.471763338583994 28.762028244998877 21.57343831556872 76-77 21.19978838674852 28.643237004127176 28.6108765023203 21.546098106804003 78-79 21.234577224843033 28.522879902681346 28.84186257065634 21.400680301819282 80-81 21.137243905388743 28.551595067743758 28.72608585957122 21.58507516729628 82-83 21.037117227650707 28.451648759984867 28.958946847270695 21.552287165093734 84-85 20.964440429004487 28.533505972045965 28.74583859802397 21.75621500092558 86-87 21.057707614417527 28.604765111459134 28.658736512724243 21.678790761399096 88-89 20.930541997856125 28.60601492956788 28.708826698136797 21.754616374439202 90-91 20.804151458613454 28.460920213106554 28.82989793188573 21.905030396394267 92-93 20.96022362245853 28.584624715093966 28.64525343707366 21.809898225373846 94-95 20.914996880094264 28.320663780606424 28.866905398147036 21.897433941152272 96-97 20.88739687503378 28.598782061315152 28.661020218142802 21.85280084550827 98-99 20.6551491571704 28.518627814331733 28.676666544193598 22.14955648430427 100-101 20.65400709919435 28.607542906113327 28.488592865615054 22.249857129077274 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 6179.0 1 4143.5 2 2468.5 3 2859.0 4 2446.0 5 2247.5 6 4209.5 7 9183.0 8 14706.0 9 17106.0 10 14735.0 11 11815.0 12 11778.0 13 12654.5 14 13641.0 15 14500.0 16 15350.5 17 16469.5 18 17643.0 19 19097.5 20 21682.0 21 25200.0 22 31838.5 23 43220.5 24 54913.0 25 67045.0 26 85319.5 27 110265.0 28 143912.5 29 173381.5 30 190262.5 31 213033.0 32 244129.0 33 271273.0 34 292704.5 35 313312.0 36 327346.0 37 337041.0 38 351444.5 39 361463.5 40 369737.0 41 370462.5 42 362547.0 43 359831.0 44 358524.0 45 352000.0 46 343508.5 47 336886.0 48 326999.0 49 319659.5 50 314828.5 51 298375.5 52 269464.0 53 245464.5 54 232928.5 55 223869.0 56 205873.5 57 180325.0 58 158606.5 59 140635.5 60 121642.5 61 103028.0 62 87356.0 63 73279.0 64 60373.0 65 51289.0 66 43242.0 67 34857.0 68 26813.0 69 19392.0 70 14718.5 71 10513.5 72 7758.0 73 5912.0 74 4448.0 75 3250.0 76 2231.0 77 1577.0 78 1288.0 79 1035.0 80 665.5 81 359.0 82 230.0 83 153.0 84 116.0 85 89.5 86 57.0 87 36.5 88 27.0 89 19.5 90 15.0 91 11.0 92 7.0 93 6.0 94 5.0 95 3.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.051215398705044386 2 0.002336345085730777 3 1.9388755898180725E-4 4 2.1327631487998798E-4 5 0.0025787045344580362 6 0.0018031542985308075 7 0.0 8 9.694377949090362E-6 9 9.694377949090362E-5 10-11 1.4541566923635544E-5 12-13 0.0 14-15 5.671211100217862E-4 16-17 0.0 18-19 6.301345666908735E-5 20-21 4.7987170847997297E-4 22-23 0.0 24-25 6.931480233599609E-4 26-27 0.0077118776585013826 28-29 0.007939695540305006 30-31 0.0011681725428653886 32-33 0.006979952123345061 34-35 0.008555288540072245 36-37 0.0049005080532651775 38-39 0.0027047314477962113 40-41 0.0021909294164944217 42-43 0.001444462314414464 44-45 8.337165036217711E-4 46-47 6.058986218181477E-4 48-49 0.0021812350385453315 50-51 0.001551100471854458 52-53 0.0023121091408580515 54-55 7.949389918254097E-4 56-57 2.2781788180362348E-4 58-59 8.240221256726808E-4 60-61 0.006432219769221455 62-63 0.0028501471170325663 64-65 0.0011148534641453915 66-67 0.005627586399446955 68-69 0.007629475445934115 70-71 0.0026514123690762137 72-73 6.010514328436024E-4 74-75 0.0035772254632143435 76-77 0.002064902503156247 78-79 0.0026804955029234847 80-81 9.64590605934491E-4 82-83 1.9388755898180725E-4 84-85 7.125367792581416E-4 86-87 0.005186492202763344 88-89 0.002811369605236205 90-91 0.001313588212101744 92-93 1.3572129128726508E-4 94-95 4.0231668488725005E-4 96-97 4.0231668488725005E-4 98-99 6.204401887417832E-4 100-101 1.9388755898180723E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.0315257E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.64157015830414 #Duplication Level Percentage of deduplicated Percentage of total 1 63.98640368323794 25.365215107866472 2 18.273174769577174 14.487546792862934 3 7.295865538530068 8.676586968335794 4 3.3684476581458163 5.341222166598507 5 1.916509776801263 3.798672838807154 6 1.1568842375169393 2.7516424599938367 7 0.7707411163629629 2.138737162673444 8 0.5486780568916473 1.7400367749273782 9 0.3818651675379424 1.3623961346971118 >10 2.0097599154620456 14.464879760522878 >50 0.1630753523083435 4.488414091706648 >100 0.1143228610069491 8.676433400771046 >500 0.00985448185781598 2.687909914616133 >1k 0.004269316174981571 2.779663344674425 >5k 4.935619609995065E-5 0.11659123821131094 >10k+ 9.87123921999013E-5 1.1240518427351769 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 43710 0.42374126015473973 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 36798 0.3567337197706271 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24345 0.23600963117060483 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 9.694377949090362E-6 0.0 4 0.0 0.0 0.0 9.694377949090362E-6 0.0 5 0.0 0.0 0.0 9.694377949090362E-6 0.0 6 0.0 0.0 0.0 1.9388755898180723E-5 0.0 7 0.0 0.0 0.0 1.9388755898180723E-5 0.0 8 0.0 0.0 0.0 1.9388755898180723E-5 0.0 9 1.9388755898180723E-5 0.0 0.0 1.9388755898180723E-5 0.0 10-11 1.9388755898180723E-5 1.4541566923635543E-5 0.0 1.9388755898180723E-5 0.0 12-13 1.9388755898180723E-5 2.9083133847271085E-5 0.0 1.9388755898180723E-5 0.0 14-15 1.9388755898180723E-5 2.9083133847271085E-5 0.0 1.9388755898180723E-5 0.0 16-17 1.9388755898180723E-5 2.9083133847271085E-5 0.0 3.3930322821816264E-5 0.0 18-19 2.4235944872725906E-5 2.9083133847271085E-5 0.0 3.877751179636145E-5 0.0 20-21 2.9083133847271085E-5 2.9083133847271085E-5 0.0 5.331907871999699E-5 0.0 22-23 2.9083133847271085E-5 3.877751179636145E-5 0.0 1.502628582109006E-4 0.0 24-25 2.9083133847271085E-5 4.362470077090663E-5 0.0 3.150672833454368E-4 0.0 26-27 2.9083133847271085E-5 4.847188974545181E-5 0.0 6.495233225890542E-4 0.0 28-29 2.9083133847271085E-5 5.816626769454217E-5 0.0 0.0016238083064726355 0.0 30-31 2.9083133847271085E-5 5.816626769454217E-5 0.0 0.0040231668488725 0.0 32-33 2.9083133847271085E-5 5.816626769454217E-5 0.0 0.009040007437526762 0.0 34-35 2.9083133847271085E-5 6.786064564363253E-5 0.0 0.016882759198340865 0.0 36-37 2.9083133847271085E-5 7.75550235927229E-5 0.0 0.02987322565012195 0.0 38-39 2.9083133847271085E-5 7.75550235927229E-5 0.0 0.048457348178528176 0.0 40-41 2.9083133847271085E-5 7.75550235927229E-5 0.0 0.06808846352543615 0.0 42-43 2.9083133847271085E-5 7.75550235927229E-5 0.0 0.08812674274620594 0.0 44-45 2.9083133847271085E-5 7.75550235927229E-5 0.0 0.1131527794217827 0.0 46-47 2.9083133847271085E-5 9.694377949090362E-5 0.0 0.1447564515358173 0.0 48-49 3.877751179636145E-5 9.694377949090362E-5 0.0 0.17713082669680455 0.0 50-51 3.877751179636145E-5 1.0663815743999398E-4 0.0 0.21142953588068625 0.0 52-53 3.877751179636145E-5 1.0663815743999398E-4 0.0 0.24537924745840067 0.0 54-55 3.877751179636145E-5 1.0663815743999398E-4 0.0 0.28441366027041304 0.0 56-57 3.877751179636145E-5 1.1148534641453915E-4 0.0 0.3321778604255813 0.0 58-59 3.877751179636145E-5 1.4541566923635544E-4 0.0 0.3859961996099564 0.0 60-61 3.877751179636145E-5 1.4541566923635544E-4 0.0 0.4435032496039604 0.0 62-63 3.877751179636145E-5 1.4541566923635544E-4 0.0 0.5039331545496152 0.0 64-65 3.877751179636145E-5 1.4541566923635544E-4 0.0 0.5676155232971898 0.0 66-67 3.877751179636145E-5 1.4541566923635544E-4 0.0 0.6447149111263055 0.0 68-69 3.877751179636145E-5 1.551100471854458E-4 0.0 0.7341794780294859 0.0 70-71 4.362470077090663E-5 1.6965161410908134E-4 0.0 0.8334596026061203 0.0 72-73 4.847188974545181E-5 1.8419318103271687E-4 0.0 0.9386096730309288 0.0 74-75 4.847188974545181E-5 1.8419318103271687E-4 0.0 1.0489268469026025 0.0 76-77 4.847188974545181E-5 1.8419318103271687E-4 0.0 1.169587922045956 0.0 78-79 4.847188974545181E-5 1.9388755898180725E-4 0.0 1.3132295201176278 0.0 80-81 4.847188974545181E-5 1.9388755898180725E-4 0.0 1.476007820260804 0.0 82-83 5.816626769454217E-5 1.9873474795635242E-4 0.0 1.6474674358573906 0.0 84-85 5.816626769454217E-5 2.0842912590544278E-4 0.0 1.8208998573666173 0.0 86-87 5.816626769454217E-5 2.1327631487998795E-4 0.0 2.0092422321615446 0.0 88-89 5.816626769454217E-5 2.1327631487998795E-4 0.0 2.2251214875208634 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 23265 0.0 54.745174 1 GTATCAA 42885 0.0 41.246304 1 ATCAACG 55900 0.0 31.404512 3 TCAACGC 56580 0.0 30.993202 4 TATCAAC 57700 0.0 30.483036 2 CAACGCA 58325 0.0 30.082071 5 AACGCAG 62825 0.0 28.08572 6 TGGTATC 10275 0.0 26.257105 2 GTGGTAT 10965 0.0 25.354866 1 CGCAGAG 70120 0.0 24.770693 8 ACGCAGA 71480 0.0 24.531958 7 GCAGAGT 75920 0.0 22.459366 9 GTACATG 39280 0.0 22.116621 1 CGATACA 2695 0.0 21.85462 2 TACATGG 38180 0.0 21.310898 2 AGAGTAC 67905 0.0 21.154907 10-11 CTGGTCG 2985 0.0 21.003366 9 GAGTACT 48405 0.0 20.954082 12-13 AGTACTT 49250 0.0 20.72476 12-13 TCGATAC 2840 0.0 20.41529 1 >>END_MODULE