FastQCFastQC Report
Wed 25 May 2016
SRR2049414_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049414_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14056026
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT561940.39978582851226935No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT472480.3361405279130815No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262060.18643960960231576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA278500.060.382891
GTATCAA543450.043.42681
ATCAACG667900.034.9695933
TCAACGC679150.034.40644
GTGGTAT107200.034.1661031
TATCAAC698950.033.4624942
CAACGCA714800.032.716645
TGGTATC111350.031.5239452
AACGCAG752150.031.2500156
CGCAGAG834400.027.7725438
ACGCAGA845500.027.5428877
GCAGAGT887900.025.3179689
GTACATG528700.023.7657851
TACATGG507150.023.349212
ACATGGG510000.022.3626773
GAGTACT602650.021.87759212-13
CAGAGTA869450.021.75382210-11
AGAGTAC836000.020.74054110-11
GTACTTT634650.020.54081314-15
AGTACTT615450.019.77083812-13
Produced by FastQC (version 0.11.5)