##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049414_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 14056026 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.50639540649683 31.0 31.0 34.0 30.0 34.0 2 31.545180835607447 33.0 31.0 34.0 30.0 34.0 3 31.568076851878335 33.0 31.0 34.0 28.0 34.0 4 35.183999801935485 37.0 35.0 37.0 33.0 37.0 5 35.20909693820999 37.0 35.0 37.0 33.0 37.0 6 35.298685560200305 37.0 35.0 37.0 33.0 37.0 7 35.24368758282035 37.0 35.0 37.0 33.0 37.0 8 35.217225409230174 37.0 35.0 37.0 33.0 37.0 9 36.805561330065835 39.0 37.0 39.0 33.0 39.0 10-11 36.74679215875099 39.0 37.0 39.0 33.0 39.0 12-13 36.7054382938677 39.0 37.0 39.0 33.0 39.0 14-15 37.88088005813307 40.0 38.0 41.0 33.0 41.0 16-17 37.873405470365526 40.0 38.0 41.0 33.0 41.0 18-19 37.91030106944879 40.0 38.0 41.0 33.0 41.0 20-21 37.87775332088885 40.0 38.0 41.0 33.0 41.0 22-23 37.775740454663364 40.0 38.0 41.0 33.0 41.0 24-25 37.67141822304541 40.0 38.0 41.0 32.0 41.0 26-27 37.5269183836171 40.0 37.0 41.0 32.0 41.0 28-29 37.37181184781531 40.0 37.0 41.0 32.0 41.0 30-31 37.17264570370032 40.0 37.0 41.0 31.5 41.0 32-33 36.9182657672944 40.0 36.0 41.0 31.0 41.0 34-35 36.71532398275302 40.0 36.0 41.0 30.5 41.0 36-37 36.521798942318405 39.0 36.0 41.0 30.0 41.0 38-39 36.30757302953196 39.0 35.0 41.0 30.0 41.0 40-41 36.08644516593809 39.0 35.0 41.0 30.0 41.0 42-43 35.84394348018423 39.0 35.0 40.0 29.0 41.0 44-45 35.55172013056891 39.0 35.0 40.0 27.5 41.0 46-47 35.1820882018858 38.5 35.0 40.0 26.5 41.0 48-49 34.87086840903681 38.0 34.5 40.0 24.5 41.0 50-51 33.48874874022003 37.0 32.5 39.0 23.0 40.0 52-53 33.770243381735355 37.5 33.5 39.5 23.5 40.0 54-55 34.82616082241168 38.5 35.0 40.0 25.5 41.0 56-57 34.94525732237547 39.0 35.0 40.5 26.0 41.0 58-59 34.79276190866466 39.0 35.0 40.5 25.0 41.0 60-61 34.54375959463934 38.0 34.0 40.0 24.5 41.0 62-63 34.28286789594726 38.0 34.0 40.0 23.5 41.0 64-65 34.014360566777555 37.5 34.0 40.0 23.0 41.0 66-67 33.69704342464932 37.0 34.0 40.0 22.0 41.0 68-69 33.3570982296134 36.5 33.0 39.5 21.5 41.0 70-71 33.02187769857568 36.0 33.0 39.0 20.5 41.0 72-73 32.757915075000575 36.0 33.0 39.0 20.0 41.0 74-75 32.55202060667788 35.0 33.0 39.0 19.5 40.0 76-77 32.20788084057329 35.0 33.0 37.5 18.0 40.0 78-79 31.82853300783593 35.0 32.0 37.0 17.0 39.0 80-81 31.481120588422364 35.0 32.0 37.0 13.5 39.0 82-83 31.128732153739612 35.0 32.0 36.0 9.0 38.5 84-85 30.80442213894596 35.0 32.0 36.0 7.0 37.0 86-87 30.505113536358003 35.0 31.5 35.5 2.0 37.0 88-89 30.211089144257414 35.0 31.0 35.0 2.0 36.5 90-91 29.939573639092586 35.0 31.0 35.0 2.0 36.0 92-93 29.687955329621616 34.0 31.0 35.0 2.0 36.0 94-95 29.425445961753343 34.0 31.0 35.0 2.0 36.0 96-97 29.16752811214208 34.0 31.0 35.0 2.0 35.0 98-99 28.852131285186864 34.0 30.0 35.0 2.0 35.0 100-101 28.32207332285811 34.0 29.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.31316257353504184 1101 2 0.30240962931174664 1101 3 0.3338296051434 1101 4 0.3027832560950685 1101 5 0.3040147187566973 1101 6 0.3108235599022535 1101 7 0.31126484744427785 1101 8 0.3395054844809309 1101 9 0.3701519324690281 1101 10-11 0.3589845690853437 1101 12-13 0.34728183276070723 1101 14-15 0.429367121123974 1101 16-17 0.41813066911546315 1101 18-19 0.3817239212098755 1101 20-21 0.3453420010876016 1101 22-23 0.3755593621076372 1101 24-25 0.3500339213486754 1101 26-27 0.3284023928767752 1101 28-29 0.33192024440165113 1101 30-31 0.3032613243897728 1101 32-33 0.24010932548429764 1101 34-35 0.29692886087773473 1101 36-37 0.3266992590142479 1101 38-39 0.20743163034168077 1101 40-41 0.21237401900098973 1101 42-43 0.10927400935176479 1101 44-45 0.07819446384745277 1101 46-47 0.004348277718669635 1101 48-49 -0.10411868012728576 1101 50-51 -0.14546525101624042 1101 52-53 -0.0760966811615944 1101 54-55 0.1655576211963421 1101 56-57 0.1522394928355979 1101 58-59 0.16540269970830934 1101 60-61 0.2029671553546919 1101 62-63 0.1825104909009383 1101 64-65 0.1477204475682825 1101 66-67 0.12722472019586917 1101 68-69 0.09860151819027152 1101 70-71 0.024359917454873425 1101 72-73 0.042339803213650384 1101 74-75 0.0105560483936884 1101 76-77 -0.025936992212756138 1101 78-79 -0.09952892057595619 1101 80-81 -0.14961990896027189 1101 82-83 -0.18290284829443948 1101 84-85 -0.26751803365679194 1101 86-87 -0.2612718685232416 1101 88-89 -0.311487395167628 1101 90-91 -0.36995311759477545 1101 92-93 -0.4444463808994641 1101 94-95 -0.5972076069852825 1101 96-97 -0.6525833273151278 1101 98-99 -0.8107048350405854 1101 100-101 -0.8632789327612613 1102 1 0.3831677040700079 1102 2 0.3820948914829607 1102 3 0.3463018677093217 1102 4 0.3509416110101782 1102 5 0.35769789934649054 1102 6 0.39238271654838286 1102 7 0.3678651678123259 1102 8 0.3660363442871457 1102 9 0.4867006143642456 1102 10-11 0.4648015952729878 1102 12-13 0.42306677611990295 1102 14-15 0.5199413739473542 1102 16-17 0.47241317004634453 1102 18-19 0.46782973230231306 1102 20-21 0.46923636641894717 1102 22-23 0.47805813645548767 1102 24-25 0.4717189843034788 1102 26-27 0.46370305953949753 1102 28-29 0.4319706511954351 1102 30-31 0.4316681442536421 1102 32-33 0.443077485704265 1102 34-35 0.40623391665723574 1102 36-37 0.368017039440943 1102 38-39 0.328628240506724 1102 40-41 0.3020495899181981 1102 42-43 0.2961735028073136 1102 44-45 0.2958694252807774 1102 46-47 0.18794245525182873 1102 48-49 0.0810580022597236 1102 50-51 0.04242756310082285 1102 52-53 0.20701900988158428 1102 54-55 0.3448537045665532 1102 56-57 0.34443111886452726 1102 58-59 0.34045987306625136 1102 60-61 0.3556773916814677 1102 62-63 0.3215373044661547 1102 64-65 0.23874267981342 1102 66-67 0.24984632494217607 1102 68-69 0.28515394443362396 1102 70-71 0.2237060427175095 1102 72-73 0.25074924532659537 1102 74-75 0.19183984149177036 1102 76-77 0.19560000078529782 1102 78-79 0.14254789850112815 1102 80-81 0.08529943578647448 1102 82-83 0.02156609202545212 1102 84-85 0.047387047063111254 1102 86-87 0.030673531276047328 1102 88-89 -0.018044371172020846 1102 90-91 -0.07649998484541598 1102 92-93 -0.14823438022532898 1102 94-95 -0.2598178417121879 1102 96-97 -0.3244393076538472 1102 98-99 -0.34791287286408945 1102 100-101 -0.3360024523739966 1103 1 0.27088662710065137 1103 2 0.25045672437162736 1103 3 0.3144819351667536 1103 4 0.29341255308565195 1103 5 0.28048147083109853 1103 6 0.28409045149664536 1103 7 0.3099832359402228 1103 8 0.28843791362275795 1103 9 0.39222167247002915 1103 10-11 0.4138606662585289 1103 12-13 0.3533520657173739 1103 14-15 0.3837751193620491 1103 16-17 0.4161869042335198 1103 18-19 0.3972821829521891 1103 20-21 0.3917610671757146 1103 22-23 0.3156176305709053 1103 24-25 0.3661388201785982 1103 26-27 0.34451129842558004 1103 28-29 0.34743825585243115 1103 30-31 0.34958155807885305 1103 32-33 0.28683845991410806 1103 34-35 0.3739438893478422 1103 36-37 0.34721973438171716 1103 38-39 0.26606582517990063 1103 40-41 0.2236682378829542 1103 42-43 0.20325029741709955 1103 44-45 0.24103497248189143 1103 46-47 0.2154318465867533 1103 48-49 0.21181277301228363 1103 50-51 0.10357642153125823 1103 52-53 0.2844400438205028 1103 54-55 0.4841323132433786 1103 56-57 0.4595280548336689 1103 58-59 0.45398469906275096 1103 60-61 0.47029519128867037 1103 62-63 0.36021833922740853 1103 64-65 0.30270370508414857 1103 66-67 0.341029615906578 1103 68-69 0.32246571247873845 1103 70-71 0.27549321573587804 1103 72-73 0.372783172076673 1103 74-75 0.2473217805384209 1103 76-77 0.1777314540402486 1103 78-79 0.238438420098376 1103 80-81 0.22294845428041654 1103 82-83 0.22297706336128442 1103 84-85 0.20958610984198955 1103 86-87 0.14289340440841514 1103 88-89 0.10795446286079269 1103 90-91 0.09778550362518601 1103 92-93 0.024002851301180073 1103 94-95 -0.02123427157017943 1103 96-97 -0.08889950344738651 1103 98-99 -0.08105576803738757 1103 100-101 -0.08121030731744128 1104 1 0.09314700804313603 1104 2 0.12786141180215793 1104 3 0.1656961345805783 1104 4 0.10311790396303167 1104 5 0.1019062530192798 1104 6 0.06148303894911322 1104 7 0.04864791379173994 1104 8 0.07701306710763589 1104 9 0.07613156785814112 1104 10-11 0.1336690122957478 1104 12-13 0.08308524369437009 1104 14-15 0.10126514095147598 1104 16-17 0.14600979385810575 1104 18-19 0.09888921230660941 1104 20-21 0.11115425001876389 1104 22-23 0.12994048493781207 1104 24-25 0.146404847071814 1104 26-27 0.0651202807131952 1104 28-29 0.10309636966940872 1104 30-31 0.07696021752830973 1104 32-33 0.018283457045455975 1104 34-35 0.05783231867460614 1104 36-37 0.03250565621195278 1104 38-39 -0.015316721896432739 1104 40-41 -0.025783568376688493 1104 42-43 0.060242348317785854 1104 44-45 0.005089797270784402 1104 46-47 0.01249804364507412 1104 48-49 -0.01767413051212685 1104 50-51 -0.11079118891198192 1104 52-53 -0.07072499996171899 1104 54-55 0.04019513102048222 1104 56-57 -0.04177436205144858 1104 58-59 0.07277178578051036 1104 60-61 0.02105880290901041 1104 62-63 0.06334220180097816 1104 64-65 0.07387792367880763 1104 66-67 0.0853075110380388 1104 68-69 0.02098370537411398 1104 70-71 0.006551090924361347 1104 72-73 -0.017543371806432617 1104 74-75 -0.04801080559820292 1104 76-77 0.00974682141951888 1104 78-79 0.02784493815950384 1104 80-81 0.006325044763908494 1104 82-83 -0.08070964768716493 1104 84-85 -0.043524667492842894 1104 86-87 -0.030297201025305753 1104 88-89 -0.06347478557097119 1104 90-91 -0.05172669259900431 1104 92-93 -0.06174533081146549 1104 94-95 -0.06942058175596699 1104 96-97 -0.13703323247008825 1104 98-99 -0.1482551764087141 1104 100-101 -0.1913963737937152 1105 1 0.2642568596467605 1105 2 0.26287702844518535 1105 3 0.29049358252564517 1105 4 0.3080957080656148 1105 5 0.27412123576565506 1105 6 0.3237341054555287 1105 7 0.32398610497960334 1105 8 0.30776308457569 1105 9 0.36127853418638267 1105 10-11 0.39932663653741685 1105 12-13 0.32241020695433775 1105 14-15 0.44686033417765003 1105 16-17 0.41445331343437175 1105 18-19 0.41059554751654304 1105 20-21 0.43508171917113003 1105 22-23 0.410049945536862 1105 24-25 0.4388725931335884 1105 26-27 0.47425548648878646 1105 28-29 0.4514243646184397 1105 30-31 0.48259530291372954 1105 32-33 0.4185807802862769 1105 34-35 0.5243366542868202 1105 36-37 0.494290227796391 1105 38-39 0.4901006905152627 1105 40-41 0.4936938952392893 1105 42-43 0.48491070801781433 1105 44-45 0.48945057677897097 1105 46-47 0.4665018525564548 1105 48-49 0.462697819663461 1105 50-51 0.38515863920576265 1105 52-53 0.4641760175110434 1105 54-55 0.5775455536448391 1105 56-57 0.6005682050231442 1105 58-59 0.6050088937050404 1105 60-61 0.5951496576666173 1105 62-63 0.6288084817088446 1105 64-65 0.6286173561294177 1105 66-67 0.6076288243178141 1105 68-69 0.5682214453396597 1105 70-71 0.5461322972218596 1105 72-73 0.5992703920552103 1105 74-75 0.5504545772585985 1105 76-77 0.5695929891185898 1105 78-79 0.5999183169290063 1105 80-81 0.629785024689486 1105 82-83 0.5998600637572054 1105 84-85 0.528881992992158 1105 86-87 0.5006479557804546 1105 88-89 0.515393617640111 1105 90-91 0.47986440775534334 1105 92-93 0.5815377875514223 1105 94-95 0.5643126008265078 1105 96-97 0.5132902756082842 1105 98-99 0.5118596141369132 1105 100-101 0.5163825283129349 1106 1 0.21689520426080122 1106 2 0.2815589865551651 1106 3 0.27313236865741075 1106 4 0.25617190724491223 1106 5 0.28277022203377555 1106 6 0.3316059337768422 1106 7 0.382197744407911 1106 8 0.3738130197120668 1106 9 0.3970440375808053 1106 10-11 0.39947284940016914 1106 12-13 0.41311200217259625 1106 14-15 0.4332627747536364 1106 16-17 0.4484728824412727 1106 18-19 0.45417088677325523 1106 20-21 0.46262852164552015 1106 22-23 0.4885198401301878 1106 24-25 0.4555499156863405 1106 26-27 0.4582322817210027 1106 28-29 0.47642497185488253 1106 30-31 0.4595943407281453 1106 32-33 0.49939499142173105 1106 34-35 0.48051136594224886 1106 36-37 0.46514696931340893 1106 38-39 0.4849792868007867 1106 40-41 0.5196577898579804 1106 42-43 0.5048080339080698 1106 44-45 0.47468095879430194 1106 46-47 0.456407289590004 1106 48-49 0.3441196640860156 1106 50-51 0.32901637481568713 1106 52-53 0.4615730695964899 1106 54-55 0.5299431125583425 1106 56-57 0.5341640910072343 1106 58-59 0.5737037264240712 1106 60-61 0.5050458706889174 1106 62-63 0.5096328737764253 1106 64-65 0.54286340618102 1106 66-67 0.559463161462503 1106 68-69 0.6055094420480955 1106 70-71 0.6105280240761033 1106 72-73 0.6189713756705544 1106 74-75 0.568506554982811 1106 76-77 0.5473762518556171 1106 78-79 0.5461249781950883 1106 80-81 0.48929820213900754 1106 82-83 0.4930117867970836 1106 84-85 0.5361443862591351 1106 86-87 0.530153025975391 1106 88-89 0.6003131060650446 1106 90-91 0.49264512199381727 1106 92-93 0.5014617575607652 1106 94-95 0.5358359463654594 1106 96-97 0.5335863982969258 1106 98-99 0.5408210681237868 1106 100-101 0.6072025800857723 1107 1 0.203544734854038 1107 2 0.22358227102785833 1107 3 0.24624365261897552 1107 4 0.24795623376382991 1107 5 0.23149955661654076 1107 6 0.25016682306499405 1107 7 0.2775473745165442 1107 8 0.30237862339684796 1107 9 0.32919822158361 1107 10-11 0.31118589124116625 1107 12-13 0.3317760418780793 1107 14-15 0.44567216056552894 1107 16-17 0.3777159168476558 1107 18-19 0.3265821481792912 1107 20-21 0.43067451072980134 1107 22-23 0.4322574079661834 1107 24-25 0.4129948506625212 1107 26-27 0.4534793761231626 1107 28-29 0.4891482909837137 1107 30-31 0.458008292341205 1107 32-33 0.53316626186934 1107 34-35 0.5371693984222148 1107 36-37 0.5239629853868877 1107 38-39 0.5619879755208927 1107 40-41 0.5310301729806355 1107 42-43 0.516127433311361 1107 44-45 0.536065725678526 1107 46-47 0.5190591231708268 1107 48-49 0.44069948550041005 1107 50-51 0.46626087775178604 1107 52-53 0.5314778595977145 1107 54-55 0.5896054281692926 1107 56-57 0.5343268465962652 1107 58-59 0.5898043928287109 1107 60-61 0.6772055193259945 1107 62-63 0.6252995809397888 1107 64-65 0.619507568944833 1107 66-67 0.6569160586383163 1107 68-69 0.6521805649651 1107 70-71 0.6375669497938645 1107 72-73 0.6377265813518349 1107 74-75 0.6806887446312757 1107 76-77 0.6762162973151788 1107 78-79 0.6281823210372437 1107 80-81 0.650570944020469 1107 82-83 0.6629762497334077 1107 84-85 0.6810597836527599 1107 86-87 0.6908911049410484 1107 88-89 0.6871662366373741 1107 90-91 0.723788762495424 1107 92-93 0.6472700006881063 1107 94-95 0.6789091497156825 1107 96-97 0.6620177263821461 1107 98-99 0.7799231788569294 1107 100-101 0.771581707602941 1108 1 0.12557234030363418 1108 2 0.1320568602935026 1108 3 0.14523759374641543 1108 4 0.1562943234571037 1108 5 0.15130474905772928 1108 6 0.18190125883496933 1108 7 0.20646154648064652 1108 8 0.19189491503068723 1108 9 0.182644833517827 1108 10-11 0.22064317172682735 1108 12-13 0.23440425860204783 1108 14-15 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-1.2156356624846971 2316 96-97 -0.9721648423544202 2316 98-99 -0.7640889294776549 2316 100-101 -0.7712370702275564 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 124487.0 3 26564.0 4 20689.0 5 18692.0 6 19612.0 7 21277.0 8 24056.0 9 28967.0 10 35441.0 11 42193.0 12 51768.0 13 62259.0 14 72060.0 15 86376.0 16 103975.0 17 131169.0 18 106540.0 19 65183.0 20 58335.0 21 58998.0 22 62223.0 23 67327.0 24 77827.0 25 91735.0 26 110664.0 27 133843.0 28 162674.0 29 201588.0 30 250195.0 31 317983.0 32 414212.0 33 554848.0 34 782836.0 35 1178523.0 36 1837718.0 37 2810655.0 38 3069755.0 39 772344.0 40 435.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.35773159507085 20.779101573256476 15.027101827133759 21.83606500453891 2 15.496368503867988 20.862583139077724 40.629598778089274 23.011449578965014 3 18.081186036249925 26.523700248914615 31.07464151556602 24.320472199269435 4 12.379557878777206 17.63812597406155 39.27692927775104 30.705386869410205 5 12.523146420555067 38.13990821755469 35.56974220079372 13.767203161096525 6 29.13439737477596 38.73323206946069 18.10226787863618 14.030102677127168 7 25.800472003364867 33.887584882915505 22.950985754525238 17.36095735919439 8 25.019415953325442 35.56566519525662 20.711905956492895 18.703012894925045 9 25.18070880699778 17.040137345168105 22.261234590985946 35.517919256848174 10-11 23.543090854228296 27.446496937056235 28.88856966549064 20.121842543224826 12-13 24.518245269967498 25.31714864551996 29.16657239259622 20.998033691916323 14-15 21.757044317893232 26.940117984162885 27.4591272097485 23.843710488195377 16-17 20.358514547068708 30.35424279839666 28.099707195961276 21.187535458573358 18-19 20.32251014091774 28.460124390324765 31.251020068110314 19.96634540064718 20-21 21.39628450395418 27.510476213920015 31.16468833810512 19.92855094402069 22-23 21.02488944555677 27.4553437952825 31.31876514826648 20.20100161089425 24-25 20.757191901860963 27.750610416289057 31.11215028576216 20.38004739608782 26-27 20.42668738020347 27.916052406452835 31.146248599650345 20.511011613693345 28-29 20.527997176418726 27.726596256656055 31.336170835204285 20.409235731720933 30-31 20.7442088391483 27.98887901327459 31.00438198863442 20.26253015894269 32-33 20.429929167454894 27.904459125741944 31.240573749235818 20.425037957567348 34-35 20.837378531986676 27.790390244339726 30.997179234303836 20.375051989369766 36-37 21.04285122767476 27.825626216576172 30.634049619233 20.49747293651607 38-39 20.768709685830547 28.04056795305019 30.745246375430966 20.4454759856883 40-41 20.669184164557635 27.91379037107748 30.831419953118406 20.585605511246477 42-43 20.714200745779554 28.047537256962123 30.52874620756895 20.709515789689366 44-45 20.781003261034332 28.24609558668753 30.243911792591287 20.728989359686853 46-47 21.190166603743002 28.31747054553465 29.74651961930448 20.74584323141787 48-49 21.21973466180301 28.300182066940017 29.558471662230605 20.921611609026368 50-51 21.167397172430604 28.47959688134295 29.243661952841492 21.109343993384947 52-53 21.113824716433736 28.646090172329693 29.189288362093606 21.05079674914296 54-55 21.11618199500194 29.001896390490163 29.141944047320006 20.739977567187886 56-57 21.07511466360067 28.71212846165257 29.100602657584414 21.11215421716235 58-59 21.1891669868082 28.364914603409474 29.315677149770707 21.13024126001162 60-61 21.25592476405171 28.5023499850667 29.255805809886485 20.985919440995104 62-63 20.975352278390222 28.56923576201095 29.31179675007553 21.143615209523293 64-65 21.135797114765886 28.548477996834087 29.23227675264585 21.08344813575418 66-67 20.989481183713135 28.480366178230295 29.245548539880957 21.28460409817562 68-69 21.255466494665757 28.758604269807364 28.921281587496694 21.06464764803019 70-71 21.329804607942023 28.510719040525156 28.90262441065678 21.25685194087604 72-73 21.153362711160916 28.576608930160948 29.047118035683177 21.222910322994963 74-75 21.093515249240376 28.80006425817786 28.944776776644883 21.16164371593688 76-77 21.245453171616465 28.86127153811367 28.76218476010989 21.131090530159977 78-79 21.216372887638727 28.81960702422934 28.91348777113446 21.050532316997465 80-81 21.22386460667416 28.798742524784505 28.857792515806757 21.119600352734576 82-83 21.08468238657235 28.815432083941484 29.053637485135287 21.046248044350875 84-85 21.022510204429395 28.787858898205158 28.881723604317912 21.307907293047535 86-87 21.12267370596537 28.861289511033224 28.82328575042895 21.192751032572456 88-89 21.04812784993089 28.805720479888308 28.907021711767065 21.23912995841374 90-91 20.923392963721827 28.717790245211873 28.929335204278484 21.429481586787823 92-93 21.015804159231912 28.863134479189984 28.837458407556376 21.283602954021724 94-95 21.02432380679388 28.594480507157222 29.0204102729268 21.360785413122098 96-97 21.06827377896315 28.78134847046261 28.78819619231804 21.362181558256204 98-99 20.94615379115052 28.69472906743737 28.857104365758936 21.50201277565318 100-101 21.038289012548766 28.779004996331935 28.665200863458484 21.517505127660815 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 7422.0 1 4903.5 2 3096.0 3 3947.5 4 3454.0 5 3226.5 6 5304.5 7 9946.5 8 16100.5 9 19777.0 10 18075.5 11 15530.0 12 15685.5 13 16846.5 14 17939.5 15 18986.0 16 20349.0 17 21999.5 18 23955.0 19 26636.5 20 30566.0 21 36471.5 22 46090.0 23 61697.5 24 78803.0 25 97311.5 26 126469.5 27 161692.5 28 205014.0 29 249184.5 30 279153.5 31 312253.5 32 350546.0 33 385037.5 34 417117.0 35 446141.5 36 464975.0 37 478954.5 38 498001.5 39 509432.5 40 518358.5 41 515803.5 42 501298.5 43 492036.5 44 481342.5 45 469550.0 46 459208.5 47 444661.0 48 424838.5 49 413511.5 50 403344.0 51 376914.5 52 343085.5 53 316078.5 54 295789.5 55 280460.0 56 254338.5 57 222990.5 58 201912.0 59 183097.0 60 163387.0 61 141693.0 62 121663.5 63 102862.0 64 85234.5 65 70801.0 66 57928.0 67 46247.0 68 36374.0 69 28579.0 70 22341.5 71 17050.5 72 13213.5 73 10249.0 74 8058.0 75 6270.5 76 4674.0 77 3577.0 78 2825.0 79 2127.0 80 1557.0 81 1147.5 82 915.0 83 746.5 84 590.5 85 494.5 86 443.5 87 346.5 88 286.0 89 249.5 90 201.0 91 168.0 92 140.0 93 118.5 94 97.5 95 97.5 96 93.0 97 67.0 98 43.5 99 36.0 100 25.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014171857678692399 2 0.004709723786794361 3 0.0042970893764709885 4 0.0059120550858400515 5 0.00282441139479964 6 0.007093043225731085 7 0.001380190958667834 8 0.004083657784924416 9 0.010735609054792586 10-11 0.0077048804548312585 12-13 0.008964126844956035 14-15 0.003525178453710885 16-17 0.0063460326553180825 18-19 0.00421171673985236 20-21 9.746709347293466E-4 22-23 0.0011987741058532477 24-25 0.007601721852250416 26-27 0.0011489734011590474 28-29 8.359404002240747E-4 30-31 5.620365244059736E-4 32-33 0.0014157628905922626 34-35 0.001906655551149379 36-37 0.001323275867588748 38-39 0.0017892681757987643 40-41 0.002870654906301397 42-43 0.002831525781184525 44-45 0.008035699421728446 46-47 0.013944197314376055 48-49 0.005378476106973621 50-51 0.005759095778565008 52-53 0.013855267484564983 54-55 0.008306046104354104 56-57 0.005556335766595764 58-59 0.007430976579013158 60-61 0.0013659621858980624 62-63 0.002458020495978024 64-65 0.0025291643598268814 66-67 0.00253983593940421 68-69 0.0020382716992697653 70-71 0.0023975482117064954 72-73 0.0013339474471660766 74-75 0.0016185229025615063 76-77 0.008352289615855862 78-79 0.006836925315875199 80-81 0.013037113050303124 82-83 0.015569834603322447 84-85 0.008697337355522819 86-87 0.005961855790534252 88-89 0.0030022710544217834 90-91 0.0027390387581810107 92-93 9.782281279217895E-4 94-95 0.0013339474471660766 96-97 0.0018248401077231929 98-99 0.006634165303905955 100-101 0.011493291204782916 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.4056026E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.63474400496755 #Duplication Level Percentage of deduplicated Percentage of total 1 64.05178336471475 26.02727820086823 2 17.65102061255841 14.344894080354326 3 7.310729639369169 8.912088821558848 4 3.652956587833283 5.937478232314608 5 2.0170338591478028 4.098082725791136 6 1.2378581061770368 3.0180028355386694 7 0.7935738194569931 2.257266830187547 8 0.5894017365843982 1.9160150945752272 9 0.4051680055589973 1.481750836640381 >10 2.0393367098551316 14.70769454841191 >50 0.1459041652918235 4.098719836308652 >100 0.09401061320671189 7.383459182121152 >500 0.007234074679031737 1.9965028683161732 >1k 0.003883282229468819 2.699389550774592 >5k 3.51411123346118E-5 0.10043487054843493 >10k+ 7.02822246692236E-5 1.020941485690119 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 56194 0.39978582851226935 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 47248 0.3361405279130815 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26206 0.18643960960231576 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.4228772769771484E-5 0.0 3 0.0 0.0 0.0 3.557193192442871E-5 0.0 4 0.0 0.0 0.0 3.557193192442871E-5 0.0 5 0.0 0.0 0.0 3.557193192442871E-5 0.0 6 0.0 0.0 0.0 3.557193192442871E-5 7.114386384885742E-6 7 0.0 0.0 0.0 4.268631830931445E-5 7.114386384885742E-6 8 0.0 0.0 0.0 5.691509107908594E-5 7.114386384885742E-6 9 0.0 0.0 0.0 7.114386384885742E-5 7.114386384885742E-6 10-11 0.0 0.0 0.0 8.53726366186289E-5 7.114386384885742E-6 12-13 0.0 0.0 0.0 9.960140938840039E-5 7.114386384885742E-6 14-15 0.0 0.0 0.0 1.0671579577328613E-4 7.114386384885742E-6 16-17 0.0 0.0 0.0 1.1383018215817187E-4 7.114386384885742E-6 18-19 0.0 0.0 0.0 1.2094456854305762E-4 7.114386384885742E-6 20-21 0.0 0.0 0.0 1.458449208901577E-4 7.114386384885742E-6 22-23 0.0 0.0 0.0 2.4188913708611526E-4 7.114386384885742E-6 24-25 0.0 0.0 0.0 3.9840563755360155E-4 7.114386384885742E-6 26-27 0.0 0.0 0.0 6.90095479333917E-4 7.114386384885742E-6 28-29 0.0 0.0 0.0 0.0016114085161766205 7.114386384885742E-6 30-31 0.0 0.0 0.0 0.00392714128445693 1.0671579577328613E-5 32-33 0.0 0.0 0.0 0.008989027197303136 1.4228772769771484E-5 34-35 0.0 3.557193192442871E-6 0.0 0.017892681757987642 1.4228772769771484E-5 36-37 0.0 7.114386384885742E-6 0.0 0.033110354235258245 1.4228772769771484E-5 38-39 0.0 7.114386384885742E-6 0.0 0.05350374280753323 1.4228772769771484E-5 40-41 3.557193192442871E-6 7.114386384885742E-6 0.0 0.07462279879106655 2.1343159154657225E-5 42-43 7.114386384885742E-6 7.114386384885742E-6 0.0 0.09610468848022904 2.1343159154657225E-5 44-45 7.114386384885742E-6 7.114386384885742E-6 0.0 0.12275873707120348 2.4900352347100097E-5 46-47 7.114386384885742E-6 7.114386384885742E-6 0.0 0.15640978467171304 2.845754553954297E-5 48-49 7.114386384885742E-6 7.114386384885742E-6 0.0 0.19319471947476477 3.557193192442871E-5 50-51 7.114386384885742E-6 7.114386384885742E-6 0.0 0.23078002274611614 3.557193192442871E-5 52-53 7.114386384885742E-6 7.114386384885742E-6 0.0 0.26832619689235065 3.557193192442871E-5 54-55 7.114386384885742E-6 7.114386384885742E-6 0.0 0.3112330611795966 3.557193192442871E-5 56-57 7.114386384885742E-6 7.114386384885742E-6 0.0 0.36395777867798484 4.268631830931445E-5 58-59 7.114386384885742E-6 7.114386384885742E-6 0.0 0.42364036606079125 4.268631830931445E-5 60-61 7.114386384885742E-6 7.114386384885742E-6 0.0 0.4881180498670108 4.268631830931445E-5 62-63 7.114386384885742E-6 7.114386384885742E-6 0.0 0.5547585071342356 4.268631830931445E-5 64-65 7.114386384885742E-6 7.114386384885742E-6 0.0 0.6254755077999998 4.268631830931445E-5 66-67 7.114386384885742E-6 7.114386384885742E-6 0.0 0.7087458432418949 4.268631830931445E-5 68-69 7.114386384885742E-6 7.114386384885742E-6 0.0 0.8083116806983709 4.268631830931445E-5 70-71 7.114386384885742E-6 7.114386384885742E-6 0.0 0.9166424421810262 4.268631830931445E-5 72-73 7.114386384885742E-6 7.114386384885742E-6 0.0 1.031468638433082 4.268631830931445E-5 74-75 7.114386384885742E-6 7.114386384885742E-6 0.0 1.1508515991646573 4.268631830931445E-5 76-77 7.114386384885742E-6 7.114386384885742E-6 0.0 1.283129385218838 4.268631830931445E-5 78-79 7.114386384885742E-6 7.114386384885742E-6 0.0 1.4390304912640315 4.268631830931445E-5 80-81 7.114386384885742E-6 1.0671579577328613E-5 0.0 1.6147736209366716 4.268631830931445E-5 82-83 7.114386384885742E-6 1.4228772769771484E-5 0.0 1.8010922859704444 4.268631830931445E-5 84-85 7.114386384885742E-6 1.7785965962214354E-5 0.0 1.9901748901147451 4.268631830931445E-5 86-87 7.114386384885742E-6 2.1343159154657225E-5 0.0 2.1962822208780777 4.268631830931445E-5 88-89 7.114386384885742E-6 2.1343159154657225E-5 0.0 2.429289046562663 4.268631830931445E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 27850 0.0 60.38289 1 GTATCAA 54345 0.0 43.4268 1 ATCAACG 66790 0.0 34.969593 3 TCAACGC 67915 0.0 34.4064 4 GTGGTAT 10720 0.0 34.166103 1 TATCAAC 69895 0.0 33.462494 2 CAACGCA 71480 0.0 32.71664 5 TGGTATC 11135 0.0 31.523945 2 AACGCAG 75215 0.0 31.250015 6 CGCAGAG 83440 0.0 27.772543 8 ACGCAGA 84550 0.0 27.542887 7 GCAGAGT 88790 0.0 25.317968 9 GTACATG 52870 0.0 23.765785 1 TACATGG 50715 0.0 23.34921 2 ACATGGG 51000 0.0 22.362677 3 GAGTACT 60265 0.0 21.877592 12-13 CAGAGTA 86945 0.0 21.753822 10-11 AGAGTAC 83600 0.0 20.740541 10-11 GTACTTT 63465 0.0 20.540813 14-15 AGTACTT 61545 0.0 19.770838 12-13 >>END_MODULE