FastQCFastQC Report
Wed 25 May 2016
SRR2049414_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049414_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14056026
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT541530.3852653659007176No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT463640.3298514103488426No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT306850.218304946220219No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA268150.060.3780361
GTATCAA524800.042.5858961
GTGGTAT101500.034.4585041
ATCAACG675800.032.6533553
TGGTATC104150.032.5625232
TCAACGC687350.032.1184774
TATCAAC706150.031.3449842
CAACGCA720750.030.6959885
AACGCAG758900.029.296016
CGCAGAG864200.025.478898
ACGCAGA875550.025.27877
GCAGAGT929000.023.2364339
GTACATG510250.022.6591571
TACATGG490600.022.2485452
ACATGGG492550.021.4751723
AGAGTAC871450.021.02124610-11
GAGTACT634350.020.79591212-13
CAGAGTA903550.020.26392210-11
AGTACTT644950.020.19637712-13
GTACTTT657950.019.73260514-15
Produced by FastQC (version 0.11.5)