##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049414_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 14056026 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07675498039062 33.0 31.0 34.0 30.0 34.0 2 32.26112010606696 34.0 31.0 34.0 30.0 34.0 3 32.33032722051026 34.0 31.0 34.0 30.0 34.0 4 35.963492881985275 37.0 35.0 37.0 35.0 37.0 5 35.851033997802794 37.0 35.0 37.0 35.0 37.0 6 35.879881909723274 37.0 35.0 37.0 35.0 37.0 7 35.8062196953819 37.0 35.0 37.0 35.0 37.0 8 35.79934264492681 37.0 35.0 37.0 35.0 37.0 9 37.40488862214683 39.0 37.0 39.0 34.0 39.0 10-11 37.4036017363656 39.0 37.0 39.0 34.0 39.0 12-13 37.382723288929604 39.0 37.0 39.0 34.0 39.0 14-15 38.609083748137635 40.0 38.0 41.0 34.0 41.0 16-17 38.59969681330982 40.0 38.0 41.0 34.0 41.0 18-19 38.64112925659073 40.0 38.0 41.0 34.0 41.0 20-21 38.63676952504214 40.0 38.0 41.0 34.0 41.0 22-23 38.55505916110286 40.0 38.0 41.0 34.0 41.0 24-25 38.459287852768625 40.0 38.0 41.0 34.0 41.0 26-27 38.27743001471397 40.0 38.0 41.0 34.0 41.0 28-29 38.09262063117983 40.0 38.0 41.0 33.0 41.0 30-31 37.897872485437915 40.0 37.0 41.0 33.0 41.0 32-33 37.64482614075985 40.0 37.0 41.0 33.0 41.0 34-35 37.457314321985464 40.0 36.5 41.0 32.5 41.0 36-37 37.25699941790091 39.5 36.0 41.0 32.0 41.0 38-39 37.390494262033954 40.0 37.0 41.0 33.0 41.0 40-41 37.28556318122918 40.0 37.0 41.0 32.0 41.0 42-43 37.08629451880638 40.0 36.0 41.0 31.5 41.0 44-45 36.834933927982206 40.0 36.0 41.0 31.0 41.0 46-47 36.497512099081206 39.5 36.0 41.0 30.0 41.0 48-49 36.212440593095096 39.0 35.5 41.0 30.0 41.0 50-51 35.97808146484647 39.0 35.0 41.0 29.0 41.0 52-53 35.797991267233 39.0 35.0 40.5 28.5 41.0 54-55 35.69205140201078 39.0 35.0 40.0 28.0 41.0 56-57 35.60158685676876 39.0 35.0 40.0 28.0 41.0 58-59 35.36682185277688 38.5 35.0 40.0 28.0 41.0 60-61 35.128062654408865 38.0 34.5 40.0 27.0 41.0 62-63 34.85815162123348 38.0 34.0 40.0 26.5 41.0 64-65 34.6084853570988 37.5 34.0 40.0 26.0 41.0 66-67 34.27646782952735 37.0 34.0 40.0 26.0 41.0 68-69 33.96392511653009 36.5 34.0 39.5 26.0 41.0 70-71 33.635939738586146 36.0 33.5 39.0 25.5 41.0 72-73 33.27111446720431 35.5 33.0 39.0 25.0 40.0 74-75 32.89775353289757 35.0 33.0 38.0 24.0 40.0 76-77 30.872480422275828 33.5 30.0 35.5 21.0 38.5 78-79 32.190312254687065 35.0 32.5 37.0 24.0 39.0 80-81 32.19758856450607 35.0 33.0 36.5 24.0 39.0 82-83 31.92341185197011 35.0 33.0 36.0 23.0 38.5 84-85 31.658123747067627 35.0 32.5 36.0 23.0 37.0 86-87 31.395940040236123 35.0 32.0 35.5 21.5 37.0 88-89 31.130632584202676 35.0 32.0 35.0 20.5 36.5 90-91 30.867193081458446 35.0 32.0 35.0 19.5 36.0 92-93 30.627395182678235 35.0 32.0 35.0 18.0 36.0 94-95 30.375091900086126 34.0 31.5 35.0 15.0 36.0 96-97 30.13797797471348 34.0 31.0 35.0 4.5 35.0 98-99 29.86213859450744 34.0 31.0 35.0 2.0 35.0 100-101 29.261977425198275 34.0 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.36619484916177214 1101 2 0.397197974037411 1101 3 0.35615042308235445 1101 4 0.3163601378749945 1101 5 0.2957875037072881 1101 6 0.30909080621348295 1101 7 0.3435182621284909 1101 8 0.3372381396450308 1101 9 0.4273321835864934 1101 10-11 0.40378733736986305 1101 12-13 0.3714380552346199 1101 14-15 0.4273856332638317 1101 16-17 0.42645351160312117 1101 18-19 0.3673743590088634 1101 20-21 0.36690380161000036 1101 22-23 0.3462029950578227 1101 24-25 0.27126954150434557 1101 26-27 0.3020712925567892 1101 28-29 0.3383205871853008 1101 30-31 0.3103572009299924 1101 32-33 0.30020210742183195 1101 34-35 0.295272707110108 1101 36-37 0.28638824103569505 1101 38-39 0.37720413923069174 1101 40-41 0.37241948895277943 1101 42-43 0.4053021438124986 1101 44-45 0.37083535573496107 1101 46-47 0.4141285349564612 1101 48-49 0.34467294453344266 1101 50-51 0.3836709252975581 1101 52-53 0.3603229653240021 1101 54-55 0.3521172805673416 1101 56-57 0.2904702529251537 1101 58-59 0.3365829212810638 1101 60-61 0.31325320490067554 1101 62-63 0.2680796581800351 1101 64-65 0.16496501234860972 1101 66-67 0.17072219308828807 1101 68-69 0.14277683000005226 1101 70-71 0.18350964659143187 1101 72-73 0.15653440903513172 1101 74-75 0.09642695682397573 1101 76-77 0.057424439802364446 1101 78-79 0.11191663077811143 1101 80-81 0.1032365997180591 1101 82-83 0.1457707369036534 1101 84-85 0.050756437054833015 1101 86-87 0.058123446123193645 1101 88-89 -0.004476527071332725 1101 90-91 -0.027800059785494824 1101 92-93 -0.06116394767476052 1101 94-95 -0.07517640718088003 1101 96-97 -0.13940270855877657 1101 98-99 -0.23009419088645444 1101 100-101 -0.32413763766261994 1102 1 0.38099407561472987 1102 2 0.37178755863502744 1102 3 0.35787225341001516 1102 4 0.2788796513310814 1102 5 0.2939215209073964 1102 6 0.3084575105975844 1102 7 0.28997343722964075 1102 8 0.2903126472418833 1102 9 0.420179141766603 1102 10-11 0.3692652584271272 1102 12-13 0.32535543565052905 1102 14-15 0.3997931954225393 1102 16-17 0.4427162226076078 1102 18-19 0.38702401754667193 1102 20-21 0.37446269482583006 1102 22-23 0.3345225246002599 1102 24-25 0.34358337442160547 1102 26-27 0.3498152378790138 1102 28-29 0.37355802619582335 1102 30-31 0.2893818875476697 1102 32-33 0.3136756790302755 1102 34-35 0.32226109623389476 1102 36-37 0.3100775219433345 1102 38-39 0.41990880231935535 1102 40-41 0.4246412231787957 1102 42-43 0.47069143657668633 1102 44-45 0.459855304114015 1102 46-47 0.4622047706296968 1102 48-49 0.25541703756947953 1102 50-51 0.3035564109242799 1102 52-53 0.3649071618736883 1102 54-55 0.39547622383931014 1102 56-57 0.398218374340658 1102 58-59 0.3122246983879222 1102 60-61 0.366568514517823 1102 62-63 0.3019315678414145 1102 64-65 0.34683532684536544 1102 66-67 0.30550091931737455 1102 68-69 0.28760290135809896 1102 70-71 0.24368961637803466 1102 72-73 0.24115613453857776 1102 74-75 0.24035668580856395 1102 76-77 0.2558221667189571 1102 78-79 0.35233188304110286 1102 80-81 0.33132696353894886 1102 82-83 0.37762187490277555 1102 84-85 0.363295006470576 1102 86-87 0.28364537559882663 1102 88-89 0.26803218289389363 1102 90-91 0.2506599337668227 1102 92-93 0.21627139629790904 1102 94-95 0.2092032890064175 1102 96-97 0.1347856872713109 1102 98-99 0.09610204999402328 1102 100-101 0.1072611596312818 1103 1 0.3177294052595414 1103 2 0.3115432425677085 1103 3 0.29326171749726626 1103 4 0.2638530132394976 1103 5 0.2710314956538653 1103 6 0.3119400141670212 1103 7 0.24669126897541815 1103 8 0.23529118446703023 1103 9 0.33038279163408646 1103 10-11 0.3253985751737076 1103 12-13 0.28662534866241174 1103 14-15 0.38352712980866244 1103 16-17 0.4182991170424728 1103 18-19 0.37765534115648336 1103 20-21 0.3282035394241021 1103 22-23 0.2690280101842575 1103 24-25 0.32853750982903307 1103 26-27 0.2851934524178148 1103 28-29 0.3094556027546318 1103 30-31 0.31109170143346176 1103 32-33 0.2801272179685057 1103 34-35 0.2549319089579143 1103 36-37 0.2684425026077477 1103 38-39 0.38010525142691165 1103 40-41 0.40056296240408784 1103 42-43 0.3908094000234641 1103 44-45 0.3486468466772763 1103 46-47 0.43335100573886365 1103 48-49 0.3773789829431493 1103 50-51 0.4210879065132005 1103 52-53 0.4183274913653605 1103 54-55 0.3675313542837273 1103 56-57 0.343018791093165 1103 58-59 0.3369450015729569 1103 60-61 0.3421960295392523 1103 62-63 0.37432736293327906 1103 64-65 0.2869760862302684 1103 66-67 0.29428703377683263 1103 68-69 0.31595570733637857 1103 70-71 0.2514903102522865 1103 72-73 0.30437212502959454 1103 74-75 0.3196827114631873 1103 76-77 0.3232852734446041 1103 78-79 0.3677819394514188 1103 80-81 0.3203993304942898 1103 82-83 0.3907900299013001 1103 84-85 0.3523604558714233 1103 86-87 0.3374868577659065 1103 88-89 0.329850203275452 1103 90-91 0.34431136517090266 1103 92-93 0.29967053468214644 1103 94-95 0.35356249269991835 1103 96-97 0.3334274183654635 1103 98-99 0.3281802501417914 1103 100-101 0.29502674862092704 1104 1 0.30224656320756793 1104 2 0.2851972502905298 1104 3 0.2968346105824793 1104 4 0.2248755924825474 1104 5 0.21422136580628148 1104 6 0.25817279266560433 1104 7 0.2706672004416646 1104 8 0.27367339785755007 1104 9 0.334007838393255 1104 10-11 0.2840735266068677 1104 12-13 0.2443131971576591 1104 14-15 0.32169802310786366 1104 16-17 0.34323453972010753 1104 18-19 0.30862504772956356 1104 20-21 0.2736377376914376 1104 22-23 0.2783145397818956 1104 24-25 0.26395914927997666 1104 26-27 0.28421838024543433 1104 28-29 0.34134847006999536 1104 30-31 0.3292953082366097 1104 32-33 0.3156303124198274 1104 34-35 0.308180026336494 1104 36-37 0.3368484685157753 1104 38-39 0.41364992656531285 1104 40-41 0.4201764690738159 1104 42-43 0.43320941072290964 1104 44-45 0.37768041222813054 1104 46-47 0.4260455829130265 1104 48-49 0.3819918451221085 1104 50-51 0.3946487419621292 1104 52-53 0.3794489529156806 1104 54-55 0.40264947441615107 1104 56-57 0.34294171073155866 1104 58-59 0.31925844287348326 1104 60-61 0.3856129961675805 1104 62-63 0.3382270152746898 1104 64-65 0.34879537085937073 1104 66-67 0.3632269234308936 1104 68-69 0.29495921225868926 1104 70-71 0.2830399852221177 1104 72-73 0.24659837361134151 1104 74-75 0.27961669981301185 1104 76-77 0.20822468072807254 1104 78-79 0.3670207136275607 1104 80-81 0.3102007324795082 1104 82-83 0.31732672507972737 1104 84-85 0.2915126504703309 1104 86-87 0.23904553606397272 1104 88-89 0.23773933401009728 1104 90-91 0.25375777773911423 1104 92-93 0.17440581712706305 1104 94-95 0.18199940963527794 1104 96-97 0.1122366742624088 1104 98-99 0.18276067200272195 1104 100-101 0.22758392590286647 1105 1 0.30121449056869665 1105 2 0.2936852153412701 1105 3 0.2843661759436813 1105 4 0.22669550025163687 1105 5 0.2547574002105861 1105 6 0.3028094947640625 1105 7 0.3017633275855047 1105 8 0.27913416520951273 1105 9 0.329730894589396 1105 10-11 0.28364837634668305 1105 12-13 0.27131330556101574 1105 14-15 0.30882451883237394 1105 16-17 0.298545632925304 1105 18-19 0.3494427796701487 1105 20-21 0.32280019372263524 1105 22-23 0.27768352065501745 1105 24-25 0.28785386251547607 1105 26-27 0.31900070904460875 1105 28-29 0.32588883265015767 1105 30-31 0.3058044728509586 1105 32-33 0.38318488113865357 1105 34-35 0.3630455616553334 1105 36-37 0.33423960523833074 1105 38-39 0.38760424736489796 1105 40-41 0.39351629570843016 1105 42-43 0.4175849152008695 1105 44-45 0.39987992193687205 1105 46-47 0.34202181758007555 1105 48-49 0.3097791430223964 1105 50-51 0.32810222054269644 1105 52-53 0.3181286039786073 1105 54-55 0.3650176300408958 1105 56-57 0.31281480916914717 1105 58-59 0.30434708896441975 1105 60-61 0.32114600244833724 1105 62-63 0.35639958063291743 1105 64-65 0.37042834597440333 1105 66-67 0.427923887689758 1105 68-69 0.36998500902679865 1105 70-71 0.3004793283438758 1105 72-73 0.3416747800513136 1105 74-75 0.33486781265137466 1105 76-77 0.35479642410007983 1105 78-79 0.36548382814955005 1105 80-81 0.3487271261555449 1105 82-83 0.3969878955471877 1105 84-85 0.40804339204088436 1105 86-87 0.44493136109102593 1105 88-89 0.42217515028983144 1105 90-91 0.42273241269332473 1105 92-93 0.44900080117002616 1105 94-95 0.4726321142048526 1105 96-97 0.5358329305821563 1105 98-99 0.5068523959767717 1105 100-101 0.5875214588382924 1106 1 0.28907960319400416 1106 2 0.2553795442036133 1106 3 0.22975044812504564 1106 4 0.20301013324192496 1106 5 0.2195757062933339 1106 6 0.26345950657375994 1106 7 0.2565562148904732 1106 8 0.2308151552111113 1106 9 0.2899842783022777 1106 10-11 0.26139642634993976 1106 12-13 0.25085567193888636 1106 14-15 0.3112234716975095 1106 16-17 0.2950706725442487 1106 18-19 0.2843841031807557 1106 20-21 0.24024010553315378 1106 22-23 0.2483422223272811 1106 24-25 0.2584334738849563 1106 26-27 0.2296051875938261 1106 28-29 0.31043291035791754 1106 30-31 0.26170104728362986 1106 32-33 0.3238242062849892 1106 34-35 0.2997900682893899 1106 36-37 0.334146263824195 1106 38-39 0.3591977641098225 1106 40-41 0.35616701697768605 1106 42-43 0.3738004682430969 1106 44-45 0.4122833294645929 1106 46-47 0.42516018131856725 1106 48-49 0.40002641329019895 1106 50-51 0.43882054545475313 1106 52-53 0.40390760866205255 1106 54-55 0.38771711279510157 1106 56-57 0.2940996449975444 1106 58-59 0.3991004998184806 1106 60-61 0.44969547642403995 1106 62-63 0.4263858088295436 1106 64-65 0.46447846685134664 1106 66-67 0.44288013864747455 1106 68-69 0.44939199209031244 1106 70-71 0.4480471185648014 1106 72-73 0.4349864505653471 1106 74-75 0.38208224809936553 1106 76-77 0.3375830957368464 1106 78-79 0.3659051395600983 1106 80-81 0.41850453581131575 1106 82-83 0.42378636137080505 1106 84-85 0.4251760546767578 1106 86-87 0.3884689340301932 1106 88-89 0.39720653015517016 1106 90-91 0.42555090461634393 1106 92-93 0.35004212342029106 1106 94-95 0.4040451372468148 1106 96-97 0.44820669819131354 1106 98-99 0.4666566839714079 1106 100-101 0.48133404756560694 1107 1 0.22626661856467933 1107 2 0.21706197392812498 1107 3 0.2037468285913704 1107 4 0.20282418562884175 1107 5 0.18260123567070252 1107 6 0.1660290076221571 1107 7 0.17265808167537244 1107 8 0.16052714139512148 1107 9 0.26528723878847416 1107 10-11 0.1519521201213223 1107 12-13 0.18293955867332556 1107 14-15 0.1644796580120058 1107 16-17 0.24407055492633845 1107 18-19 0.18974479082439188 1107 20-21 0.18266392736961734 1107 22-23 0.18755166786776556 1107 24-25 0.2059114483594513 1107 26-27 0.26057103629170797 1107 28-29 0.2824397815668007 1107 30-31 0.2617669447711535 1107 32-33 0.3015245415202159 1107 34-35 0.2590006077247864 1107 36-37 0.2819999379558098 1107 38-39 0.31279522869377274 1107 40-41 0.3441160801970611 1107 42-43 0.3451035269862288 1107 44-45 0.22299092458020908 1107 46-47 0.22660855867552954 1107 48-49 0.14791681276512492 1107 50-51 0.21493263241261928 1107 52-53 0.24222234497529627 1107 54-55 0.268744940803856 1107 56-57 0.32278028915643375 1107 58-59 0.30828162993776687 1107 60-61 0.37859337972760443 1107 62-63 0.35339896751400346 1107 64-65 0.3400944603693006 1107 66-67 0.3612539646465578 1107 68-69 0.36589292238500803 1107 70-71 0.3541713012897816 1107 72-73 0.36166886092888717 1107 74-75 0.3623441657455402 1107 76-77 0.30647450585450287 1107 78-79 0.41537376316925645 1107 80-81 0.4240219674622523 1107 82-83 0.4444087246484578 1107 84-85 0.43083509247518137 1107 86-87 0.4250217202676403 1107 88-89 0.34991311172391093 1107 90-91 0.4448875614218224 1107 92-93 0.47664506425509856 1107 94-95 0.5322593102525879 1107 96-97 0.5017701431277395 1107 98-99 0.5820917250069897 1107 100-101 0.6789266704545334 1108 1 0.12639940045286835 1108 2 0.12407132382447372 1108 3 0.12761036213564836 1108 4 0.1252265127766563 1108 5 0.10948990259738878 1108 6 0.12718887972384607 1108 7 0.1489433621070475 1108 8 0.13538832422431568 1108 9 0.18336504862563885 1108 10-11 0.10067059083072394 1108 12-13 0.09661149311778416 1108 14-15 0.14732701169113938 1108 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-1.4479971853964102 2316 64-65 -1.6765114345923138 2316 66-67 -1.2412417975242533 2316 68-69 -1.239828249222846 2316 70-71 -1.1392398869309304 2316 72-73 -1.1916848339109265 2316 74-75 -1.0405274528096164 2316 76-77 -0.9630042142320718 2316 78-79 -0.990396967908751 2316 80-81 -0.8555236106572082 2316 82-83 -0.9561758793010391 2316 84-85 -1.1011363326991415 2316 86-87 -1.2144448132441212 2316 88-89 -0.9467358790980249 2316 90-91 -0.8838369602303935 2316 92-93 -0.9383519369024604 2316 94-95 -1.1813891348657428 2316 96-97 -1.1542663300049831 2316 98-99 -1.155169229668843 2316 100-101 -1.4391813621575764 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 43.0 4 121.0 5 251.0 6 709.0 7 1846.0 8 4796.0 9 9765.0 10 16262.0 11 25053.0 12 35848.0 13 49822.0 14 63657.0 15 80714.0 16 100508.0 17 129210.0 18 109810.0 19 64525.0 20 51077.0 21 49429.0 22 50801.0 23 55623.0 24 66265.0 25 81694.0 26 103315.0 27 130974.0 28 167180.0 29 211630.0 30 266870.0 31 338735.0 32 436692.0 33 576772.0 34 802637.0 35 1193285.0 36 1875214.0 37 2942775.0 38 3214839.0 39 746961.0 40 309.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.60533747604328 22.327597957829063 16.26079853937443 22.80626602675322 2 14.510456114498933 21.978682456364645 39.586554880363174 23.924306548773252 3 16.799972908329913 26.979845803718717 31.681559615084854 24.53862167286652 4 12.059571441285879 18.56785399143156 38.58737306218833 30.785201505094236 5 12.29520962135306 38.317935139690775 35.75368572345945 13.633169515496718 6 29.188602761665994 39.284441551528474 17.69487750636019 13.83207818044534 7 25.61990138606744 33.88291968156576 22.87905557374467 17.61812335862213 8 25.144368685715296 35.878063970570345 20.39294036593273 18.584626977781628 9 24.845269012368234 16.85090816681379 22.157756313172275 36.1460665076457 10-11 23.45357657189294 27.695754613369466 28.81314753160078 20.03752128313682 12-13 24.33859145811138 25.371436803790548 29.23170668805726 21.05826505004082 14-15 21.506500231005177 27.05534491342535 27.471890575685958 23.96626427988351 16-17 20.192503201118154 30.405147941530558 28.194779235610408 21.20756962174088 18-19 20.1949606419429 28.450934160471427 31.443688188613038 19.910417008972633 20-21 21.219847475311884 27.504159447306105 31.3609663207492 19.915026756632813 22-23 20.80558189064249 27.44029500230008 31.505220607873092 20.248902499184336 24-25 20.63673775221387 27.70343943139151 31.31114502009401 20.348677796300606 26-27 20.340914091574223 27.862958729446785 31.31742887567322 20.478698303305773 28-29 20.441470798544284 27.671848708106396 31.547533075535668 20.339147417813653 30-31 20.596973427860398 27.947451272754865 31.22927622722139 20.226299072163346 32-33 20.31411029605488 27.835897996330576 31.448734879381192 20.401256828233354 34-35 20.657371444151202 27.768088954602838 31.211674941209814 20.362864660036145 36-37 20.910411692217345 27.776358588728367 30.79995103649556 20.51327868255872 38-39 20.645201751564166 27.96538876085234 30.962030933362776 20.427378554220716 40-41 20.505679430364417 27.896629721573195 31.02159881779116 20.57609203027123 42-43 20.56448695393083 28.001799678610524 30.73112014697375 20.702593220484893 44-45 20.62786587512333 28.2153756468095 30.438677920385977 20.718080557681194 46-47 20.964923186375863 28.33308699615376 29.936380977789632 20.76560883968074 48-49 20.98764118983002 28.259786299131413 29.761541242776428 20.991031268262137 50-51 21.00105842330145 28.43681827582652 29.366511919303424 21.195611381568607 52-53 20.96411385981706 28.627532379201188 29.31923016312593 21.089123597855817 54-55 20.98454074206772 28.955662363898927 29.24890192128381 20.81089497274955 56-57 21.034112404703862 28.630807947915997 29.17820460342645 21.15687504395369 58-59 21.028975675392925 28.35329664342982 29.403221513113646 21.214506168063604 60-61 21.107811063752948 28.534721224419275 29.29253050413315 21.064937207694626 62-63 20.8435802583013 28.52950589690352 29.372775959428953 21.254137885366227 64-65 20.948144121890625 28.582038264413185 29.305888354197673 21.163929259498516 66-67 20.814688300780055 28.529348181555488 29.3060122308117 21.349951286852757 68-69 21.078420264164443 28.713074450550923 28.99640940668497 21.21209587859966 70-71 21.143050191855274 28.465566188450808 28.988099943033575 21.403283676660347 72-73 20.922304186398097 28.590081593632583 29.07512572533849 21.41248849463083 74-75 20.890354139313875 28.760317460543323 28.98832716660802 21.361001233534786 76-77 20.96449650151513 28.845081124402842 28.88331820604803 21.30710416803399 78-79 20.90747444578811 28.812828570986454 29.014099838445834 21.2655971447796 80-81 20.893995646663303 28.77792469133036 28.952947466488236 21.375132195518106 82-83 20.73220675650847 28.75649033215525 29.16957008224778 21.341732829088496 84-85 20.667535529142654 28.790113152274703 28.970442786907103 21.571908531675536 86-87 20.72056429263159 28.854595140641116 28.907439856541274 21.517400710186024 88-89 20.627157831566812 28.77590887496525 28.983974929623407 21.61295836384453 90-91 20.507232544818912 28.681560214006407 29.032891023738816 21.77831621743587 92-93 20.578908218658487 28.76360258837314 28.953499999164055 21.703989193804315 94-95 20.506593842488606 28.543084711892032 29.097159499200142 21.85316194641922 96-97 20.49607874914965 28.7353121171112 28.923306915621783 21.845302218117364 98-99 20.268274384966645 28.61557156064569 29.03360819923527 22.082545855152393 100-101 20.25558295345764 28.777975506372766 28.810950693131666 22.155490847037925 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 6885.0 1 4716.5 2 3219.5 3 3950.0 4 3333.5 5 2963.5 6 5200.5 7 11052.0 8 17391.0 9 20171.0 10 18144.0 11 15194.0 12 15346.5 13 16803.0 14 18267.5 15 19506.5 16 20834.5 17 22452.0 18 24408.0 19 27149.0 20 30991.0 21 36588.0 22 46333.5 23 62069.0 24 79066.0 25 97910.0 26 126987.5 27 162527.5 28 206123.0 29 250034.0 30 280789.5 31 313224.5 32 351414.0 33 387200.5 34 418960.0 35 448772.5 36 468520.5 37 481551.0 38 499038.5 39 511949.0 40 522516.5 41 520148.0 42 505604.0 43 496376.5 44 487275.5 45 474884.5 46 463669.0 47 448752.5 48 426844.5 49 416130.5 50 407271.0 51 378622.0 52 340699.5 53 313214.5 54 294910.0 55 277568.5 56 249837.0 57 218722.5 58 197794.0 59 180430.5 60 161353.0 61 138516.0 62 118053.0 63 98923.0 64 81073.0 65 67118.0 66 54643.5 67 43107.5 68 33836.0 69 26274.5 70 19697.5 71 14758.0 72 11413.5 73 8690.5 74 6663.0 75 5048.5 76 3549.5 77 2620.5 78 1950.0 79 1300.5 80 844.5 81 528.5 82 365.0 83 262.5 84 173.5 85 109.5 86 82.5 87 62.0 88 44.5 89 37.5 90 22.0 91 11.0 92 11.0 93 8.5 94 5.0 95 3.0 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.051586415676806524 2 0.0019849138013831222 3 3.2014738731985843E-4 4 2.0631720516168652E-4 5 0.0026536661215623815 6 0.0016718808004481493 7 0.0 8 0.0 9 1.2805895492794335E-4 10-11 2.4900352347100097E-5 12-13 7.114386384885743E-6 14-15 4.5532072863268755E-4 16-17 0.0 18-19 6.758667065641456E-5 20-21 5.122358197117734E-4 22-23 0.0 24-25 6.972098657188027E-4 26-27 0.007665751329714388 28-29 0.007655079750137058 30-31 0.0011205158556195044 32-33 0.006851154088644969 34-35 0.008334503649893646 36-37 0.004691937820832147 38-39 0.0025647362917513104 40-41 0.0021556590746203796 42-43 0.001301932708434091 44-45 7.825825023374317E-4 46-47 6.296231950623882E-4 48-49 0.002162773461005266 50-51 0.001515364299980663 52-53 0.002283718029548323 54-55 7.968112751072031E-4 56-57 2.810182622029868E-4 58-59 8.217116274543033E-4 60-61 0.005979641756496466 62-63 0.0027497103377583394 64-65 0.0010387004121933184 66-67 0.005588350505327751 68-69 0.007872068534876073 70-71 0.0026963524398716963 72-73 6.296231950623882E-4 74-75 0.0034433630102846994 76-77 0.002123644335888394 78-79 0.002621651382830396 80-81 0.0010031284802688896 82-83 1.6718808004481496E-4 84-85 7.114386384885742E-4 86-87 0.0049409413443031475 88-89 0.0027995110424525395 90-91 0.0013161614812038623 92-93 1.1027298896572902E-4 94-95 5.086786265193306E-4 96-97 3.699480920140586E-4 98-99 5.762652971757451E-4 100-101 1.7785965962214357E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.4056026E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.21789224090235 #Duplication Level Percentage of deduplicated Percentage of total 1 61.84668432412281 24.254966012805532 2 18.82766335295502 14.767625450483523 3 7.688045629586851 9.045268351328323 4 3.872749947675148 6.075243604955366 5 2.1514255038300267 4.218718678676751 6 1.3012466287099187 3.061929003814984 7 0.8610170996690308 2.3637093082676026 8 0.589627441641634 1.8499156374864438 9 0.4213996724552064 1.4873766250229845 >10 2.1706594298687505 15.237560893175313 >50 0.1597196326211004 4.317671945834846 >100 0.09831851311438683 7.516284854673344 >500 0.007278032680933517 1.9594380254834856 >1k 0.004055196138503013 2.708136562706916 >5k 3.653164360539068E-5 0.10458666654784608 >10k+ 7.306328721078136E-5 1.031568378736714 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 54153 0.3852653659007176 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 46364 0.3298514103488426 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30685 0.218304946220219 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.1343159154657225E-5 0.0 10-11 0.0 0.0 0.0 3.557193192442871E-5 0.0 12-13 0.0 0.0 0.0 4.268631830931445E-5 0.0 14-15 0.0 0.0 0.0 5.691509107908594E-5 0.0 16-17 0.0 0.0 0.0 5.691509107908594E-5 0.0 18-19 0.0 0.0 0.0 6.402947746397169E-5 0.0 20-21 0.0 0.0 0.0 8.53726366186289E-5 0.0 22-23 0.0 3.557193192442871E-6 0.0 1.7074527323725783E-4 0.0 24-25 0.0 7.114386384885742E-6 0.0 3.3437616008962986E-4 0.0 26-27 0.0 7.114386384885742E-6 0.0 6.367375814472739E-4 0.0 28-29 0.0 7.114386384885742E-6 0.0 0.0015580506182899774 0.0 30-31 0.0 7.114386384885742E-6 0.0 0.003834654261453415 0.0 32-33 0.0 7.114386384885742E-6 0.0 0.008885868594722292 0.0 34-35 0.0 7.114386384885742E-6 0.0 0.017697036132403282 0.0 36-37 0.0 7.114386384885742E-6 0.0 0.03269771982493487 0.0 38-39 0.0 7.114386384885742E-6 0.0 0.052902577158010375 0.0 40-41 0.0 1.7785965962214354E-5 0.0 0.07386511664107623 0.0 42-43 0.0 3.557193192442871E-5 0.0 0.09527230527319741 0.0 44-45 0.0 3.557193192442871E-5 0.0 0.12188366754586254 0.0 46-47 0.0 3.557193192442871E-5 0.0 0.15537108425951973 0.0 48-49 0.0 3.557193192442871E-5 0.0 0.1920670892327604 0.0 50-51 0.0 3.557193192442871E-5 0.0 0.22967729285645885 0.0 52-53 0.0 3.557193192442871E-5 0.0 0.26713453717288227 0.0 54-55 0.0 3.557193192442871E-5 0.0 0.3097674975843101 0.0 56-57 0.0 3.557193192442871E-5 0.0 0.36211159541110693 0.0 58-59 0.0 3.557193192442871E-5 0.0 0.4213175189061261 0.0 60-61 0.0 3.557193192442871E-5 0.0 0.48525095215390185 0.0 62-63 0.0 4.624351150175732E-5 0.0 0.5517491216934288 0.0 64-65 0.0 5.691509107908594E-5 0.0 0.6220250304033301 0.0 66-67 0.0 5.691509107908594E-5 0.0 0.7046266135250461 0.0 68-69 0.0 5.691509107908594E-5 0.0 0.8034418832179167 0.0 70-71 0.0 5.691509107908594E-5 0.0 0.9109971765846193 0.0 72-73 0.0 5.691509107908594E-5 0.0 1.0248451447087534 7.114386384885742E-6 74-75 0.0 5.691509107908594E-5 0.0 1.1434882092563003 7.114386384885742E-6 76-77 0.0 6.402947746397169E-5 0.0 1.2753782612525049 7.114386384885742E-6 78-79 0.0 6.758667065641456E-5 0.0 1.4309129763988768 7.114386384885742E-6 80-81 0.0 7.114386384885742E-5 0.0 1.6057917081257533 7.114386384885742E-6 82-83 0.0 7.114386384885742E-5 0.0 1.7912530896001473 7.114386384885742E-6 84-85 0.0 7.114386384885742E-5 0.0 1.9795566684353032 7.114386384885742E-6 86-87 0.0 7.114386384885742E-5 0.0 2.1846430847524045 7.114386384885742E-6 88-89 0.0 7.114386384885742E-5 0.0 2.416938471798501 7.114386384885742E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 26815 0.0 60.378036 1 GTATCAA 52480 0.0 42.585896 1 GTGGTAT 10150 0.0 34.458504 1 ATCAACG 67580 0.0 32.653355 3 TGGTATC 10415 0.0 32.562523 2 TCAACGC 68735 0.0 32.118477 4 TATCAAC 70615 0.0 31.344984 2 CAACGCA 72075 0.0 30.695988 5 AACGCAG 75890 0.0 29.29601 6 CGCAGAG 86420 0.0 25.47889 8 ACGCAGA 87555 0.0 25.2787 7 GCAGAGT 92900 0.0 23.236433 9 GTACATG 51025 0.0 22.659157 1 TACATGG 49060 0.0 22.248545 2 ACATGGG 49255 0.0 21.475172 3 AGAGTAC 87145 0.0 21.021246 10-11 GAGTACT 63435 0.0 20.795912 12-13 CAGAGTA 90355 0.0 20.263922 10-11 AGTACTT 64495 0.0 20.196377 12-13 GTACTTT 65795 0.0 19.732605 14-15 >>END_MODULE