FastQCFastQC Report
Wed 25 May 2016
SRR2049413_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049413_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13343689
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT512360.3839717787187636No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT428310.32098320037285044No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT242530.18175633439898067No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA247150.058.986371
GTATCAA485850.043.2248731
ATCAACG587350.035.4056853
TCAACGC594000.034.9538574
TATCAAC612600.034.0067182
CAACGCA621350.033.4759455
GTGGTAT104500.032.95621
TGGTATC103050.031.4808042
AACGCAG663650.031.4425666
CGCAGAG730150.028.1448928
ACGCAGA745300.027.725757
GCAGAGT780350.025.549059
GTACATG430550.024.5372891
TACATGG420300.023.799732
ACATGGG433000.022.2142353
GAGTACT545450.022.09454212-13
CAGAGTA766100.021.72365410-11
AGAGTAC731000.021.00895910-11
GTACTTT596150.019.9426314-15
AGTACTT564150.019.83389512-13
Produced by FastQC (version 0.11.5)