##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049413_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13343689 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.487073402265295 31.0 31.0 34.0 30.0 34.0 2 31.518371268994652 33.0 31.0 34.0 30.0 34.0 3 31.546731042667435 33.0 31.0 34.0 28.0 34.0 4 35.16304831445037 37.0 35.0 37.0 33.0 37.0 5 35.187763593710855 37.0 35.0 37.0 33.0 37.0 6 35.276953921812776 37.0 35.0 37.0 33.0 37.0 7 35.22049667074825 37.0 35.0 37.0 33.0 37.0 8 35.19666832762664 37.0 35.0 37.0 32.0 37.0 9 36.784481263015046 39.0 37.0 39.0 33.0 39.0 10-11 36.725935534019115 39.0 37.0 39.0 32.5 39.0 12-13 36.68720404080161 39.0 37.0 39.0 33.0 39.0 14-15 37.86742594195653 40.0 38.0 41.0 33.0 41.0 16-17 37.86142411592476 40.0 38.0 41.0 33.0 41.0 18-19 37.895898278204776 40.0 38.0 41.0 33.0 41.0 20-21 37.867202727821365 40.0 38.0 41.0 33.0 41.0 22-23 37.76180882213307 40.0 38.0 41.0 32.5 41.0 24-25 37.656497577244195 40.0 38.0 41.0 32.0 41.0 26-27 37.51461653520252 40.0 37.0 41.0 32.0 41.0 28-29 37.36078351346468 40.0 37.0 41.0 32.0 41.0 30-31 37.17669094356141 40.0 37.0 41.0 31.5 41.0 32-33 36.925961141630324 40.0 36.0 41.0 31.0 41.0 34-35 36.72431072097079 40.0 36.0 41.0 30.5 41.0 36-37 36.53476291301453 39.0 36.0 41.0 30.0 41.0 38-39 36.32891987365713 39.0 35.5 41.0 30.0 41.0 40-41 36.11526741218265 39.0 35.0 41.0 30.0 41.0 42-43 35.88787943124274 39.0 35.0 40.0 29.0 41.0 44-45 35.61411990342401 39.0 35.0 40.0 27.5 41.0 46-47 35.26518641134397 39.0 35.0 40.0 26.5 41.0 48-49 34.97956487145346 38.5 35.0 40.0 25.5 41.0 50-51 33.619833615726506 37.0 32.5 39.0 23.5 40.0 52-53 33.900839752785004 37.5 33.5 39.5 23.5 40.0 54-55 34.94825306555032 39.0 35.0 40.0 26.0 41.0 56-57 35.08119227748789 39.0 35.0 41.0 26.0 41.0 58-59 34.93557504974824 39.0 35.0 41.0 26.0 41.0 60-61 34.69534474312164 38.5 34.5 40.0 25.0 41.0 62-63 34.44222028855739 38.0 34.0 40.0 24.5 41.0 64-65 34.17900473399823 38.0 34.0 40.0 23.5 41.0 66-67 33.87546775857861 37.0 34.0 40.0 23.0 41.0 68-69 33.542103761560995 37.0 33.5 40.0 22.0 41.0 70-71 33.21608005102637 36.0 33.0 39.0 21.5 41.0 72-73 32.95825966867183 36.0 33.0 39.0 20.5 41.0 74-75 32.75600776516899 35.5 33.0 39.0 20.0 40.5 76-77 32.40636236351132 35.0 33.0 38.0 19.5 40.0 78-79 32.02404878441037 35.0 33.0 37.0 18.0 39.0 80-81 31.67083649056869 35.0 32.0 37.0 17.0 39.0 82-83 31.31516468197063 35.0 32.0 36.0 12.5 39.0 84-85 30.981965706784685 35.0 32.0 36.0 8.5 37.0 86-87 30.673405944937716 35.0 32.0 36.0 6.5 37.0 88-89 30.372319641142717 35.0 31.0 35.0 2.0 37.0 90-91 30.09795184824826 35.0 31.0 35.0 2.0 36.0 92-93 29.84032616467605 34.5 31.0 35.0 2.0 36.0 94-95 29.578468443022018 34.0 31.0 35.0 2.0 36.0 96-97 29.324804894658442 34.0 31.0 35.0 2.0 35.5 98-99 29.01714222356351 34.0 30.5 35.0 2.0 35.0 100-101 28.491157655128205 34.0 29.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3290940389379493 1101 2 0.34940728626838435 1101 3 0.346638709751371 1101 4 0.33281371240061475 1101 5 0.36323934993797025 1101 6 0.3368483803207738 1101 7 0.35379512957663906 1101 8 0.3364380504729567 1101 9 0.4036651525975117 1101 10-11 0.3648023016239179 1101 12-13 0.415128865873271 1101 14-15 0.47370851269231906 1101 16-17 0.47185458653032697 1101 18-19 0.4348561720265707 1101 20-21 0.40146848543704294 1101 22-23 0.4265119383385567 1101 24-25 0.3758284606387008 1101 26-27 0.3734337851724945 1101 28-29 0.37425148998357827 1101 30-31 0.3365066402220762 1101 32-33 0.29864531144461637 1101 34-35 0.32274567301183055 1101 36-37 0.32932544423688626 1101 38-39 0.2509002229651145 1101 40-41 0.21790722921522132 1101 42-43 0.2094687153340189 1101 44-45 0.15558987038534866 1101 46-47 0.12583216269234043 1101 48-49 0.020803876727136128 1101 50-51 -0.060183508848169254 1101 52-53 0.09007696108017882 1101 54-55 0.3339761844634168 1101 56-57 0.3621908955191131 1101 58-59 0.3181619057045637 1101 60-61 0.3536614154421258 1101 62-63 0.30629366031509875 1101 64-65 0.25466671307130184 1101 66-67 0.20429277545159863 1101 68-69 0.18692839955584617 1101 70-71 0.14648150948728755 1101 72-73 0.0955363047259965 1101 74-75 0.07120425699788058 1101 76-77 0.033739371922429484 1101 78-79 -0.030622984732126213 1101 80-81 -0.12684576441444406 1101 82-83 -0.16797808757962684 1101 84-85 -0.18541058181424575 1101 86-87 -0.20465496467229727 1101 88-89 -0.27530352444164663 1101 90-91 -0.2814669660520188 1101 92-93 -0.345438246343722 1101 94-95 -0.4818705497847411 1101 96-97 -0.5114986153338386 1101 98-99 -0.6458183801703505 1101 100-101 -0.7190493703078715 1102 1 0.3327468283538657 1102 2 0.34009288807269655 1102 3 0.32253388545508344 1102 4 0.3223962456784406 1102 5 0.35629974849618407 1102 6 0.34730218556396864 1102 7 0.3373850711124291 1102 8 0.32361297672063927 1102 9 0.4189455246886098 1102 10-11 0.4079478628903743 1102 12-13 0.37285416902389557 1102 14-15 0.46449519750937185 1102 16-17 0.3862190574798632 1102 18-19 0.364068769079708 1102 20-21 0.34996520834568656 1102 22-23 0.3572950603484273 1102 24-25 0.36866818788761435 1102 26-27 0.3628402542356923 1102 28-29 0.37659922486002984 1102 30-31 0.3673769098778976 1102 32-33 0.3252169668797791 1102 34-35 0.3292054451649804 1102 36-37 0.2707216345389867 1102 38-39 0.2487156634091079 1102 40-41 0.24071652763311135 1102 42-43 0.17097290064837978 1102 44-45 0.2487612622438249 1102 46-47 0.1855216037069951 1102 48-49 0.030447037731072157 1102 50-51 0.052516309814450324 1102 52-53 0.21151441792518 1102 54-55 0.37891871439637725 1102 56-57 0.34498704198455954 1102 58-59 0.29708978780531226 1102 60-61 0.34290079691345454 1102 62-63 0.3466282305662389 1102 64-65 0.19928681903577683 1102 66-67 0.2209873616552116 1102 68-69 0.1813234707083069 1102 70-71 0.15081205792341734 1102 72-73 0.10961521346436598 1102 74-75 0.10267147206739224 1102 76-77 0.09788004174553322 1102 78-79 0.06409500242295252 1102 80-81 -0.012041455750726016 1102 82-83 -0.06129275017766034 1102 84-85 -0.03638360691883591 1102 86-87 -0.05587854315927743 1102 88-89 -0.05662300926222841 1102 90-91 -0.12958250891592016 1102 92-93 -0.22082214097679653 1102 94-95 -0.3034956108449798 1102 96-97 -0.3321434483897754 1102 98-99 -0.3959066822963955 1102 100-101 -0.5352172567711655 1103 1 0.24891907953035286 1103 2 0.22199939875931562 1103 3 0.24685024555898494 1103 4 0.23774768511013633 1103 5 0.23985210752370278 1103 6 0.24048179017798788 1103 7 0.25505187933954687 1103 8 0.28044444572514493 1103 9 0.30847259795203996 1103 10-11 0.31206398353594267 1103 12-13 0.27034551183538724 1103 14-15 0.3537185582901756 1103 16-17 0.4018045773712302 1103 18-19 0.3696531064750559 1103 20-21 0.3495030465996578 1103 22-23 0.3059591375068109 1103 24-25 0.3477951336310454 1103 26-27 0.3377840278798345 1103 28-29 0.34516846653215794 1103 30-31 0.3293154727950096 1103 32-33 0.29127776809654193 1103 34-35 0.30499021852337904 1103 36-37 0.3225892846847671 1103 38-39 0.3051341950981552 1103 40-41 0.2179558924512719 1103 42-43 0.2220732287881333 1103 44-45 0.17400118293914346 1103 46-47 0.22810959331582836 1103 48-49 0.19314563753096792 1103 50-51 0.15708497503423047 1103 52-53 0.33268737883044963 1103 54-55 0.4379775672111137 1103 56-57 0.4756025716133152 1103 58-59 0.5008607826000144 1103 60-61 0.5014722168981294 1103 62-63 0.45086633151233 1103 64-65 0.4459963273902332 1103 66-67 0.42645235133524295 1103 68-69 0.4003524524948574 1103 70-71 0.3233775668151395 1103 72-73 0.3917060889182977 1103 74-75 0.40606611988008723 1103 76-77 0.2925245051905776 1103 78-79 0.3107626812815383 1103 80-81 0.3393898441210119 1103 82-83 0.2842574005992482 1103 84-85 0.2719479877330926 1103 86-87 0.22668652706456882 1103 88-89 0.2371882944286412 1103 90-91 0.21038458463604215 1103 92-93 0.1846015942726389 1103 94-95 0.12221131774937177 1103 96-97 0.11603464949546449 1103 98-99 0.11762826756946865 1103 100-101 0.05719352315373172 1104 1 0.08895889287468961 1104 2 0.10628615585088852 1104 3 0.16710848425144675 1104 4 0.1138623678347912 1104 5 0.06632687862907005 1104 6 0.08550254733906826 1104 7 0.056185966361120165 1104 8 0.07790427924762611 1104 9 0.05086073631961341 1104 10-11 0.08569324214177954 1104 12-13 0.06223287695794966 1104 14-15 0.08990905457215348 1104 16-17 0.11217590719343207 1104 18-19 0.047901550645931934 1104 20-21 0.08734818850376769 1104 22-23 0.09054895144075914 1104 24-25 0.13809838079064463 1104 26-27 0.09954522625957907 1104 28-29 0.013841092720042525 1104 30-31 0.031149166482073554 1104 32-33 -0.003356657833904819 1104 34-35 -0.011582814239204708 1104 36-37 -0.051221632131529304 1104 38-39 -0.018044951971326384 1104 40-41 -0.0021202949535563675 1104 42-43 0.010208338630434355 1104 44-45 0.03797543406562909 1104 46-47 -0.0582433216044862 1104 48-49 -0.09766233485454023 1104 50-51 -0.15980177572851773 1104 52-53 -0.1161479066494806 1104 54-55 -0.018999553972570027 1104 56-57 -0.0678952660239247 1104 58-59 0.0021729250959765523 1104 60-61 -0.03794983749679659 1104 62-63 -0.06718749930324464 1104 64-65 -0.07285431892336902 1104 66-67 0.0023687506646652423 1104 68-69 -0.020703416128910135 1104 70-71 -0.05056354338127278 1104 72-73 -0.07033315641538707 1104 74-75 -0.019073730048695836 1104 76-77 -0.04357085510466163 1104 78-79 0.039498053331769256 1104 80-81 -0.04906189927945448 1104 82-83 -0.06698010672251797 1104 84-85 -0.054054997447643416 1104 86-87 -0.08082941384071773 1104 88-89 -0.10639798557187774 1104 90-91 -0.10290756024270209 1104 92-93 -0.11555618520671729 1104 94-95 -0.10901389558902252 1104 96-97 -0.1801571312156618 1104 98-99 -0.1843890921323066 1104 100-101 -0.25154495562008705 1105 1 0.31099601110124553 1105 2 0.29486149788602134 1105 3 0.2964717509265107 1105 4 0.3397807339198806 1105 5 0.31647317369794337 1105 6 0.35999746309529 1105 7 0.33014254502237605 1105 8 0.35246181111838126 1105 9 0.4386757598888096 1105 10-11 0.41408773543223987 1105 12-13 0.3729984010451517 1105 14-15 0.4799154209309364 1105 16-17 0.45585820061608473 1105 18-19 0.46619204234193035 1105 20-21 0.4792161675640827 1105 22-23 0.39886683638720655 1105 24-25 0.461188273783236 1105 26-27 0.5135630668415985 1105 28-29 0.4883736611022087 1105 30-31 0.4844969507061734 1105 32-33 0.43371556562338753 1105 34-35 0.5199053321077898 1105 36-37 0.48443052731088443 1105 38-39 0.4490305719668797 1105 40-41 0.4751134215577295 1105 42-43 0.48753136965697763 1105 44-45 0.4105594223674629 1105 46-47 0.37958030352300653 1105 48-49 0.34153719670922555 1105 50-51 0.30173088646167656 1105 52-53 0.4004342225477302 1105 54-55 0.5398840633102608 1105 56-57 0.5130515722532962 1105 58-59 0.5555017569121148 1105 60-61 0.5508579058899983 1105 62-63 0.5167527564729113 1105 64-65 0.5223130664718099 1105 66-67 0.5212996173972257 1105 68-69 0.4576476485004406 1105 70-71 0.44156463157290915 1105 72-73 0.5452208125309781 1105 74-75 0.4661108808061911 1105 76-77 0.45908524725047783 1105 78-79 0.4711605143804505 1105 80-81 0.4244922162689271 1105 82-83 0.46189701899142577 1105 84-85 0.474074279930651 1105 86-87 0.4359640710128474 1105 88-89 0.43870324254128334 1105 90-91 0.42942547227298533 1105 92-93 0.47811984218009584 1105 94-95 0.49057677465194516 1105 96-97 0.46951452076368483 1105 98-99 0.48646609964671583 1105 100-101 0.44429965994213205 1106 1 0.337733999730478 1106 2 0.3340346347437517 1106 3 0.30004861291826757 1106 4 0.3092652803466933 1106 5 0.34596903078954 1106 6 0.32943957024979653 1106 7 0.40863614498035616 1106 8 0.3758701957655646 1106 9 0.39672871913440844 1106 10-11 0.49038668810649 1106 12-13 0.446756046390945 1106 14-15 0.47936766682814635 1106 16-17 0.49675150475396634 1106 18-19 0.4966449516514331 1106 20-21 0.48962466258316795 1106 22-23 0.5338801305127063 1106 24-25 0.5197872853670091 1106 26-27 0.525082890296872 1106 28-29 0.5651207837487959 1106 30-31 0.6134323793009386 1106 32-33 0.652519124787915 1106 34-35 0.6481768435077271 1106 36-37 0.6404739569395588 1106 38-39 0.6210738941923566 1106 40-41 0.6388655220423658 1106 42-43 0.7033613986663312 1106 44-45 0.684163109886093 1106 46-47 0.6805771976701678 1106 48-49 0.5948479814040368 1106 50-51 0.5666731770525146 1106 52-53 0.6418196351826637 1106 54-55 0.6820236622612796 1106 56-57 0.706191174768982 1106 58-59 0.7469281366053551 1106 60-61 0.7406203333709058 1106 62-63 0.8015390598105583 1106 64-65 0.8201632577056728 1106 66-67 0.81594635815879 1106 68-69 0.8288892056318318 1106 70-71 0.8339660644454625 1106 72-73 0.8188647431406082 1106 74-75 0.8249660305870279 1106 76-77 0.8216995117217252 1106 78-79 0.8241473352951161 1106 80-81 0.782242788939751 1106 82-83 0.8267815803633098 1106 84-85 0.8431684021582022 1106 86-87 0.7821054693095633 1106 88-89 0.8411202195215601 1106 90-91 0.8286103435812286 1106 92-93 0.8285648039619637 1106 94-95 0.8648946658473164 1106 96-97 0.9165791489826915 1106 98-99 0.9331140355723413 1106 100-101 0.9416956648808714 1107 1 0.21641695198975341 1107 2 0.18467624019853446 1107 3 0.17865228580659576 1107 4 0.22529792796579073 1107 5 0.21506110900535447 1107 6 0.2326988264684573 1107 7 0.2632682314490111 1107 8 0.3209809286947518 1107 9 0.3351179558384487 1107 10-11 0.3527259464672241 1107 12-13 0.3722439980714256 1107 14-15 0.4387895679769258 1107 16-17 0.37282515348336887 1107 18-19 0.3438540339624012 1107 20-21 0.4345093111522047 1107 22-23 0.4664948826447457 1107 24-25 0.42866046769202626 1107 26-27 0.4422463010674349 1107 28-29 0.46144162747052064 1107 30-31 0.43611061105101356 1107 32-33 0.4346748924745043 1107 34-35 0.37029778611256603 1107 36-37 0.3898510618033555 1107 38-39 0.4981362853693483 1107 40-41 0.39770869023395505 1107 42-43 0.3893876526525162 1107 44-45 0.48236055942766853 1107 46-47 0.47504899520583166 1107 48-49 0.3336230615819389 1107 50-51 0.33745733012762713 1107 52-53 0.37604578998469407 1107 54-55 0.4730323548561941 1107 56-57 0.4735655219922208 1107 58-59 0.45800274762117965 1107 60-61 0.5286077497758654 1107 62-63 0.5305276289917558 1107 64-65 0.5784422215782641 1107 66-67 0.6374207535183842 1107 68-69 0.6523547614168308 1107 70-71 0.6268305947613655 1107 72-73 0.5792829061283555 1107 74-75 0.6016400608959813 1107 76-77 0.58364719191691 1107 78-79 0.5500966092701347 1107 80-81 0.576636153761708 1107 82-83 0.5875423626883567 1107 84-85 0.580063709749421 1107 86-87 0.5413143665493152 1107 88-89 0.5797908383232837 1107 90-91 0.5481023643985274 1107 92-93 0.5433955573954101 1107 94-95 0.549822649505316 1107 96-97 0.5298544823352991 1107 98-99 0.5834819304503469 1107 100-101 0.6147166670226483 1108 1 0.09483911183542304 1108 2 0.08896758369016311 1108 3 0.10325019292293192 1108 4 0.047860903041623715 1108 5 0.05899665001564358 1108 6 0.08226763554509375 1108 7 0.1279046000502504 1108 8 0.1490028412676878 1108 9 0.11379082400180351 1108 10-11 0.16127077085526764 1108 12-13 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-0.7741463486176876 2316 92-93 -0.868376459794046 2316 94-95 -1.1321027128529444 2316 96-97 -0.9462467801698331 2316 98-99 -0.7849610540662866 2316 100-101 -0.7865536119351049 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 116139.0 3 24971.0 4 19734.0 5 17831.0 6 18489.0 7 20506.0 8 22799.0 9 27570.0 10 33185.0 11 40270.0 12 49421.0 13 58943.0 14 67135.0 15 77902.0 16 93208.0 17 115475.0 18 95379.0 19 60459.0 20 55046.0 21 55835.0 22 58583.0 23 64262.0 24 73494.0 25 86670.0 26 104174.0 27 126434.0 28 154027.0 29 189349.0 30 234677.0 31 299202.0 32 385282.0 33 515649.0 34 725011.0 35 1088540.0 36 1700694.0 37 2658437.0 38 3013833.0 39 794656.0 40 418.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.399689397465565 20.786804788855484 14.91181481373217 21.901690999946783 2 15.409619461210344 21.068510921974017 40.64207184936283 22.879797767452807 3 17.95888221129525 26.470670674721745 31.31634781344407 24.25409930053893 4 12.327526922078412 17.682668722783383 39.66820835449307 30.321596000645133 5 12.488616983015426 37.77422944160245 35.76381870720103 13.973334868181084 6 28.901191020002226 38.95589857124816 18.24889368882687 13.894016719922744 7 25.41555229820766 33.856264323914246 23.341090164677098 17.387093213200995 8 24.620685179153508 35.55176450996759 21.257158548461387 18.570391762417508 9 24.795804665315423 17.068531699565792 22.65624783347995 35.479415801638844 10-11 23.047391785705614 27.444978869331614 29.503296636091125 20.00433270887164 12-13 24.17220001065016 25.208214985183268 29.651108997817094 20.968476006349476 14-15 21.461426523411923 27.105655058002103 27.740932606762247 23.691985811823727 16-17 20.065688406776022 30.39650886956875 28.401959957329932 21.1358427663253 18-19 19.971311843684138 28.60641017176096 31.43855526627659 19.98372271827832 20-21 21.158852888350836 27.757898450833906 31.274199865313367 19.809048795501894 22-23 20.758289836002277 27.730765428871607 31.391361017493185 20.11958371763293 24-25 20.587676385301464 27.80080389273521 31.26024225755498 20.351277464408348 26-27 20.25674691242673 27.95533970228791 31.277807719464327 20.510105665821026 28-29 20.26975438306188 27.928630563470097 31.445695657605604 20.355919395862415 30-31 20.579486784599453 28.302696026770853 30.96159643365077 20.156220754978925 32-33 20.24891634657994 28.060674998851503 31.422348182664557 20.268060471904 34-35 20.560258571886152 27.911002150419012 31.16844779077309 20.360291486921746 36-37 20.90177932441287 28.03999724360578 30.71371462379172 20.344508808189623 38-39 20.60551377078731 28.235345960681595 30.85674910576882 20.30239116276227 40-41 20.483979503441162 28.05696006681399 30.92400290692424 20.53505752282061 42-43 20.566015231501183 28.12711306361338 30.67534810547904 20.6315235994064 44-45 20.72183582255612 28.379065923229383 30.41032013318825 20.488778121026243 46-47 20.93769362284555 28.56362068429867 29.972828670570106 20.525857022285678 48-49 20.984131497530573 28.47307717702794 29.78521353524436 20.757577790197132 50-51 20.95292880945786 28.605098173589898 29.545531195026925 20.896441821925315 52-53 20.841592132294284 28.794784613895946 29.48262873364233 20.880994520167434 54-55 20.95029515232486 29.10879377200939 29.37175972122979 20.569151354435963 56-57 20.82683490312424 28.84224541979647 29.337007978585874 20.993911698493424 58-59 20.7577286495041 28.525478015864486 29.69154128404337 21.02525205058804 60-61 20.996667936070747 28.704402578882508 29.438402419929087 20.860527065117658 62-63 20.766194230112177 28.757978020409976 29.518812288851272 20.957015460626575 64-65 20.855098804785772 28.63080739200901 29.546579720193876 20.967514083011338 66-67 20.70428302712446 28.66947960492359 29.46739434038382 21.15884302756813 68-69 21.07224503892913 28.961402645477285 29.125805842275227 20.840546473318355 70-71 21.052814796389786 28.646818646843382 29.128488822495495 21.171877734271337 72-73 20.849367188642 28.822861120392567 29.325191447932685 21.002580243032746 74-75 20.92753905365175 28.980934907451548 29.135557349941305 20.955968688955394 76-77 21.01467292778139 29.021210351187708 28.98644567268566 20.977671048345236 78-79 21.058898515936267 28.962384514872248 29.18427589673059 20.7944410724609 80-81 21.028655819529696 28.94340418398777 29.099925127330195 20.928014869152335 82-83 20.844211898675145 28.956404264165258 29.342718412472625 20.856665424686973 84-85 20.830186289387388 28.903652019975944 29.131850570515113 21.134311120121552 86-87 20.984200180387997 29.004409871598057 29.005369182896484 21.006020765117462 88-89 20.802495905153265 28.967382907768176 29.145232525203387 21.084888661875173 90-91 20.71428908675897 28.87112153396871 29.12706227676569 21.287527102506623 92-93 20.808701453048773 28.952218876747665 29.072273004713246 21.16680666549032 94-95 20.72069027099535 28.784151519918332 29.275896085236326 21.219262123849994 96-97 20.730188804951762 28.900933663872465 29.135621791450834 21.233255739724942 98-99 20.707358210640404 28.84714969939472 29.066016319851794 21.37947577011308 100-101 20.769348816371302 28.901061693496782 28.906072138794958 21.423517351336958 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 8208.0 1 5325.0 2 3406.0 3 4538.5 4 3890.0 5 3519.0 6 5512.0 7 10278.5 8 16542.0 9 19834.0 10 17851.5 11 15041.5 12 15239.5 13 16533.5 14 17441.0 15 18299.0 16 19386.5 17 20858.0 18 22920.0 19 25586.0 20 29786.5 21 36021.0 22 46061.5 23 62580.0 24 80300.0 25 98687.0 26 125762.5 27 161485.5 28 209510.5 29 254470.0 30 280893.5 31 314215.5 32 356026.5 33 391171.0 34 422097.5 35 448658.0 36 463698.5 37 471085.5 38 479903.5 39 484762.5 40 490156.0 41 484023.5 42 463156.5 43 447126.0 44 433416.0 45 420677.5 46 407391.0 47 390270.0 48 376076.0 49 369277.0 50 363365.5 51 347408.5 52 321615.5 53 299031.5 54 285284.0 55 272525.0 56 246367.0 57 216483.5 58 195439.5 59 174972.5 60 153107.5 61 131970.5 62 112627.0 63 93557.5 64 75784.5 65 62109.5 66 51181.5 67 41352.5 68 32212.5 69 24363.0 70 18414.5 71 13805.5 72 10619.0 73 8220.0 74 6421.0 75 4979.0 76 3630.0 77 2692.0 78 2146.0 79 1709.5 80 1241.0 81 822.0 82 629.5 83 496.0 84 374.0 85 309.0 86 275.5 87 235.0 88 192.5 89 166.0 90 143.0 91 126.0 92 98.5 93 79.0 94 67.0 95 56.5 96 43.5 97 28.0 98 24.0 99 19.0 100 12.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014081563201900163 2 0.0047513097764793535 3 0.004189246317116653 4 0.005523210260670794 5 0.002592986092526587 6 0.006924621819348457 7 0.0013639406613868174 8 0.004129292881451299 9 0.01079161841976383 10-11 0.007471696919794818 12-13 0.008764442876329027 14-15 0.0034922876275069064 16-17 0.0063513170907985046 18-19 0.004136787060909468 20-21 9.217840733548272E-4 22-23 0.001124126918725399 24-25 0.00740799639440038 26-27 0.0011953216235780076 28-29 7.943830225659486E-4 30-31 5.358338312591068E-4 32-33 0.0012740105078887856 34-35 0.0018210856083351462 36-37 0.0012702634181597009 38-39 0.001824832698064231 40-41 0.0028178114762716666 42-43 0.0026641807973791956 44-45 0.008022519109970264 46-47 0.014002874317589387 48-49 0.005365832492049237 50-51 0.005942884310328276 52-53 0.01392418543327861 54-55 0.008146173071030058 56-57 0.005418291748256423 58-59 0.006977081075555643 60-61 0.0011803332646616689 62-63 0.002323195632032491 64-65 0.002300713093657983 66-67 0.002592986092526587 68-69 0.0020571522612674803 70-71 0.0024431025033632005 72-73 0.0013377110332832248 74-75 0.001637478211609998 76-77 0.008419710621253238 78-79 0.00674101442262331 80-81 0.012773828886449616 82-83 0.015632858349741215 84-85 0.008554605851500285 86-87 0.005871689605475667 88-89 0.00294895961678963 90-91 0.0027428696816899733 92-93 9.330253425420812E-4 94-95 0.0013189755846378015 96-97 0.001768626352127961 98-99 0.006441247244296536 100-101 0.011702161223931404 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.3343689E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.44671823314025 #Duplication Level Percentage of deduplicated Percentage of total 1 67.90999677690682 32.22106482287361 2 17.10354767900822 16.230144150259658 3 6.470078957503067 9.209520397284535 4 3.00907400843464 5.71082746483457 5 1.5457960753762992 3.6671475417136645 6 0.9141561168652409 2.602422461880403 7 0.613404400916336 2.037281804127191 8 0.426234612189259 1.6178746836604463 9 0.31012896238467746 1.324314134478175 >10 1.5349027924991567 13.115303136033246 >50 0.10235975420850403 3.3213985421674592 >100 0.05446727177541381 4.642957867165891 >500 0.003465511896270628 1.1416991137700387 >1k 0.0022606163724246506 1.9921883454809524 >5k 6.323183183770532E-5 0.18990904611241205 >10k+ 6.323183183770532E-5 0.9759464881574667 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 51236 0.3839717787187636 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 42831 0.32098320037285044 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24253 0.18175633439898067 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.4988358916338652E-5 0.0 5 0.0 0.0 0.0 2.9976717832677304E-5 0.0 6 0.0 0.0 0.0 2.9976717832677304E-5 0.0 7 0.0 0.0 0.0 3.747089729084663E-5 0.0 8 0.0 0.0 0.0 4.496507674901596E-5 0.0 9 0.0 0.0 0.0 5.245925620718528E-5 0.0 10-11 0.0 7.494179458169326E-6 0.0 5.995343566535461E-5 0.0 12-13 0.0 7.494179458169326E-6 0.0 7.119470485260861E-5 0.0 14-15 0.0 7.494179458169326E-6 0.0 8.993015349803191E-5 0.0 16-17 0.0 7.494179458169326E-6 0.0 1.0491851241437057E-4 0.0 18-19 0.0 1.4988358916338652E-5 0.0 1.1990687133070922E-4 0.0 20-21 0.0 1.4988358916338652E-5 0.0 1.6487194807972516E-4 0.0 22-23 0.0 1.4988358916338652E-5 0.0 2.4730792211958777E-4 0.0 24-25 0.0 1.4988358916338652E-5 0.0 4.0468569074114365E-4 0.0 26-27 0.0 1.4988358916338652E-5 0.0 7.606592150041867E-4 0.0 28-29 0.0 1.4988358916338652E-5 0.0 0.001689937467817183 0.0 30-31 0.0 1.4988358916338652E-5 0.0 0.00400189183066242 0.0 32-33 0.0 1.4988358916338652E-5 0.0 0.009292782528129965 0.0 34-35 0.0 2.248253837450798E-5 0.0 0.018263315339558647 0.0 36-37 0.0 3.747089729084663E-5 0.0 0.032120053157713734 0.0 38-39 0.0 4.496507674901596E-5 0.0 0.05148875996735236 0.0 40-41 0.0 4.496507674901596E-5 0.0 0.0712921291855648 0.0 42-43 0.0 4.496507674901596E-5 0.0 0.09175123910636707 0.0 44-45 0.0 4.496507674901596E-5 0.0 0.11859164283580051 0.0 46-47 0.0 4.496507674901596E-5 0.0 0.15069670763459792 0.0 48-49 0.0 4.496507674901596E-5 0.0 0.18493011939951537 0.0 50-51 0.0 4.496507674901596E-5 0.0 0.2201115448658913 0.0 52-53 0.0 4.496507674901596E-5 0.0 0.25503067405123125 0.0 54-55 0.0 4.496507674901596E-5 0.0 0.29552921984317826 0.0 56-57 0.0 4.496507674901596E-5 0.0 0.345882611622618 0.0 58-59 0.0 4.496507674901596E-5 0.0 0.40262479139014706 0.0 60-61 0.0 4.496507674901596E-5 0.0 0.4641295222033427 0.0 62-63 0.0 4.496507674901596E-5 0.0 0.5255967821192475 0.0 64-65 0.0 4.496507674901596E-5 0.0 0.5932804638957039 0.0 66-67 0.0 4.496507674901596E-5 0.0 0.6738990994169602 0.0 68-69 0.0 4.496507674901596E-5 0.0 0.7683519902179974 0.0 70-71 0.0 4.496507674901596E-5 0.0 0.8724349016227821 0.0 72-73 0.0 4.496507674901596E-5 0.0 0.9829290835540306 0.0 74-75 0.0 5.620634593626995E-5 0.0 1.0994298503210018 0.0 76-77 0.0 6.744761512352394E-5 0.0 1.2301021104433714 0.0 78-79 0.0 6.744761512352394E-5 0.0 1.3822189650852923 0.0 80-81 0.0 7.119470485260861E-5 0.0 1.5523480800549234 0.0 82-83 0.0 7.494179458169326E-5 0.0 1.73264679654929 0.0 84-85 0.0 7.494179458169326E-5 0.0 1.9186148598037618 0.0 86-87 0.0 7.494179458169326E-5 0.0 2.1197848660891303 0.0 88-89 0.0 7.494179458169326E-5 0.0 2.3490280686248006 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 24715 0.0 58.98637 1 GTATCAA 48585 0.0 43.224873 1 ATCAACG 58735 0.0 35.405685 3 TCAACGC 59400 0.0 34.953857 4 TATCAAC 61260 0.0 34.006718 2 CAACGCA 62135 0.0 33.475945 5 GTGGTAT 10450 0.0 32.9562 1 TGGTATC 10305 0.0 31.480804 2 AACGCAG 66365 0.0 31.442566 6 CGCAGAG 73015 0.0 28.144892 8 ACGCAGA 74530 0.0 27.72575 7 GCAGAGT 78035 0.0 25.54905 9 GTACATG 43055 0.0 24.537289 1 TACATGG 42030 0.0 23.79973 2 ACATGGG 43300 0.0 22.214235 3 GAGTACT 54545 0.0 22.094542 12-13 CAGAGTA 76610 0.0 21.723654 10-11 AGAGTAC 73100 0.0 21.008959 10-11 GTACTTT 59615 0.0 19.94263 14-15 AGTACTT 56415 0.0 19.833895 12-13 >>END_MODULE