FastQCFastQC Report
Wed 25 May 2016
SRR2049413_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049413_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13343689
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT499250.3741469094491036No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT424430.31807545874308074No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT287020.215097938808376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA241950.057.491811
GTATCAA466550.042.8022651
ATCAACG595450.033.3358353
TCAACGC604300.032.9025274
TATCAAC613300.032.45142
CAACGCA627750.031.7112645
AACGCAG675900.029.6125076
GTGGTAT106950.029.505041
TGGTATC106250.028.9246252
CGCAGAG754750.026.2728188
ACGCAGA772250.025.8250587
GCAGAGT812850.023.997689
GTACATG420800.023.3213271
TACATGG412700.022.5471882
AGAGTAC765150.021.16406410-11
ACATGGG421500.021.1519763
GAGTACT574100.020.54206712-13
CAGAGTA799500.020.19238710-11
AGTACTT588550.020.0538612-13
GTACTTT609950.019.13238714-15
Produced by FastQC (version 0.11.5)