##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049413_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13343689 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07412777680895 33.0 31.0 34.0 30.0 34.0 2 32.26515231282743 34.0 31.0 34.0 30.0 34.0 3 32.33642008592976 34.0 31.0 34.0 30.0 34.0 4 35.967604161038224 37.0 35.0 37.0 35.0 37.0 5 35.8562576660772 37.0 35.0 37.0 35.0 37.0 6 35.881621866336964 37.0 35.0 37.0 35.0 37.0 7 35.80576862964957 37.0 35.0 37.0 35.0 37.0 8 35.80064808165118 37.0 35.0 37.0 35.0 37.0 9 37.41134029727461 39.0 37.0 39.0 34.0 39.0 10-11 37.40875630419744 39.0 37.0 39.0 34.0 39.0 12-13 37.390157249618156 39.0 37.0 39.0 34.0 39.0 14-15 38.62461550175517 40.0 38.0 41.0 34.0 41.0 16-17 38.61719817510735 40.0 38.0 41.0 34.0 41.0 18-19 38.65713746775723 40.0 38.0 41.0 34.0 41.0 20-21 38.65215046603679 40.0 38.0 41.0 34.0 41.0 22-23 38.568556079207184 40.0 38.0 41.0 34.0 41.0 24-25 38.47312564763762 40.0 38.0 41.0 34.0 41.0 26-27 38.29609911472008 40.0 38.0 41.0 34.0 41.0 28-29 38.11348664525979 40.0 38.0 41.0 33.5 41.0 30-31 37.92678542642893 40.0 37.0 41.0 33.0 41.0 32-33 37.67821184981155 40.0 37.0 41.0 33.0 41.0 34-35 37.49309733612647 40.0 36.5 41.0 32.5 41.0 36-37 37.29723163511979 39.5 36.0 41.0 32.0 41.0 38-39 37.43337198581292 40.0 37.0 41.0 33.0 41.0 40-41 37.33917393458435 40.0 37.0 41.0 32.0 41.0 42-43 37.1479829528401 40.0 36.5 41.0 31.5 41.0 44-45 36.91572274353817 40.0 36.0 41.0 31.0 41.0 46-47 36.598144598543925 40.0 36.0 41.0 30.5 41.0 48-49 36.3341262674812 39.0 36.0 41.0 30.0 41.0 50-51 36.11107426889221 39.0 35.5 41.0 29.5 41.0 52-53 35.93706552962978 39.0 35.0 41.0 29.0 41.0 54-55 35.84013453850731 39.0 35.0 40.0 29.0 41.0 56-57 35.760249545684104 39.0 35.0 41.0 28.5 41.0 58-59 35.535957635103756 39.0 35.0 40.0 28.0 41.0 60-61 35.30722534825264 38.0 35.0 40.0 28.0 41.0 62-63 35.043437463208264 38.0 34.5 40.0 27.0 41.0 64-65 34.80301376928074 38.0 34.0 40.0 27.0 41.0 66-67 34.48390583743371 37.0 34.0 40.0 26.0 41.0 68-69 34.17674868621413 37.0 34.0 40.0 26.0 41.0 70-71 33.85533835508306 36.0 34.0 39.0 26.0 41.0 72-73 33.49243694903261 36.0 33.0 39.0 25.5 40.5 74-75 33.12011528446144 35.0 33.0 38.5 25.0 40.0 76-77 31.0821574903312 33.5 30.0 36.0 22.0 38.5 78-79 32.398880924158235 35.0 32.5 37.0 24.5 39.0 80-81 32.40392986527189 35.0 33.0 37.0 25.0 39.0 82-83 32.12256108486941 35.0 33.0 36.0 24.0 39.0 84-85 31.84589160463797 35.0 33.0 36.0 24.0 37.0 86-87 31.57066175628044 35.0 32.5 36.0 23.5 37.0 88-89 31.298831042899756 35.0 32.0 35.0 22.5 37.0 90-91 31.02896740174325 35.0 32.0 35.0 20.0 36.0 92-93 30.78495410826796 35.0 32.0 35.0 19.5 36.0 94-95 30.530207875798062 34.0 32.0 35.0 18.0 36.0 96-97 30.29716902125042 34.0 32.0 35.0 11.0 35.5 98-99 30.0205835882416 34.0 31.0 35.0 2.0 35.0 100-101 29.421579032604853 34.0 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.365750776786669 1101 2 0.3899041576227589 1101 3 0.3510221325430436 1101 4 0.2748120731338446 1101 5 0.308262816142431 1101 6 0.32636106274159005 1101 7 0.3426233358291455 1101 8 0.33131256685241794 1101 9 0.40183930324672446 1101 10-11 0.38906951632228726 1101 12-13 0.34822225456980505 1101 14-15 0.42280243408318796 1101 16-17 0.4123163204044644 1101 18-19 0.3585377617798571 1101 20-21 0.3463512767801191 1101 22-23 0.2766096419674966 1101 24-25 0.2493311310667039 1101 26-27 0.25875023641159345 1101 28-29 0.27411597430088364 1101 30-31 0.30388091773314585 1101 32-33 0.27121859164805784 1101 34-35 0.25621579077467516 1101 36-37 0.2094089752581354 1101 38-39 0.29582882325614435 1101 40-41 0.3047431902935429 1101 42-43 0.35617593215767585 1101 44-45 0.3156589502691034 1101 46-47 0.3653138262408788 1101 48-49 0.24924224791773497 1101 50-51 0.3046575489697929 1101 52-53 0.323116797976958 1101 54-55 0.2918861099973 1101 56-57 0.2622645512857886 1101 58-59 0.30180471019802724 1101 60-61 0.34514629253204276 1101 62-63 0.368431036900958 1101 64-65 0.25596951612669017 1101 66-67 0.24196011788934868 1101 68-69 0.1730609835183543 1101 70-71 0.12570801903964224 1101 72-73 0.12302803161335873 1101 74-75 0.08371594527849169 1101 76-77 0.05697296174564315 1101 78-79 0.1152714218444757 1101 80-81 0.10249744977249975 1101 82-83 0.13895825666405415 1101 84-85 0.109532197736808 1101 86-87 0.07183028394360136 1101 88-89 -6.428775205975512E-4 1101 90-91 -0.008655059867923143 1101 92-93 -0.050539971151774665 1101 94-95 -0.077393833412291 1101 96-97 -0.1544478528158706 1101 98-99 -0.22074037625710474 1101 100-101 -0.25681644900758727 1102 1 0.3658667528326234 1102 2 0.35232599860039926 1102 3 0.34151017664159156 1102 4 0.29080995100261475 1102 5 0.27961488466353757 1102 6 0.2745774702030701 1102 7 0.27653143863769003 1102 8 0.2650418092523381 1102 9 0.42539470542630653 1102 10-11 0.3593442461019407 1102 12-13 0.2735811442681353 1102 14-15 0.3708779517702965 1102 16-17 0.37609705165646545 1102 18-19 0.36223598634787635 1102 20-21 0.3452561528048008 1102 22-23 0.34173877236605676 1102 24-25 0.33885620195801636 1102 26-27 0.3077167535126861 1102 28-29 0.3552728766421396 1102 30-31 0.29989641330416106 1102 32-33 0.29986609109698037 1102 34-35 0.28942268253599224 1102 36-37 0.3069067152613414 1102 38-39 0.3892740141097022 1102 40-41 0.3940402813685395 1102 42-43 0.4227301134205632 1102 44-45 0.32426154308258504 1102 46-47 0.3031905683777367 1102 48-49 0.2043734991034185 1102 50-51 0.2825137927245649 1102 52-53 0.32768459087215973 1102 54-55 0.36088352876222274 1102 56-57 0.2740632898350768 1102 58-59 0.26703544204527674 1102 60-61 0.2826530249991137 1102 62-63 0.3082128152016068 1102 64-65 0.3173263048152677 1102 66-67 0.2663934409755768 1102 68-69 0.2515203147129128 1102 70-71 0.18133943852500067 1102 72-73 0.14964909447621721 1102 74-75 0.14544713594157344 1102 76-77 0.17645161869695158 1102 78-79 0.23720212490894 1102 80-81 0.3058612543398098 1102 82-83 0.3084431108452961 1102 84-85 0.28755820046877645 1102 86-87 0.2489244340273622 1102 88-89 0.2363108493811623 1102 90-91 0.17471036529181205 1102 92-93 0.1571301881731948 1102 94-95 0.16276117558161474 1102 96-97 0.20016430475717328 1102 98-99 0.11435391524446104 1102 100-101 0.11739759246575687 1103 1 0.3317415132124708 1103 2 0.36703990160937394 1103 3 0.31084420598691764 1103 4 0.27959297650939163 1103 5 0.2575377824561187 1103 6 0.3168865762777031 1103 7 0.3020816865110447 1103 8 0.29324530999535625 1103 9 0.37959423654294255 1103 10-11 0.2959672513479248 1103 12-13 0.31217050571104465 1103 14-15 0.3910733306267602 1103 16-17 0.4311084680023285 1103 18-19 0.42973945870174646 1103 20-21 0.36066416247560795 1103 22-23 0.32739249210779064 1103 24-25 0.36635799882022724 1103 26-27 0.32391386253535615 1103 28-29 0.3379968469343311 1103 30-31 0.3267299438742839 1103 32-33 0.3174758667209616 1103 34-35 0.3135309354796334 1103 36-37 0.32071191112337516 1103 38-39 0.3898579191946965 1103 40-41 0.43552569748229075 1103 42-43 0.4838116412608855 1103 44-45 0.44468609556043504 1103 46-47 0.4941936376657665 1103 48-49 0.472465505962127 1103 50-51 0.4185798140312258 1103 52-53 0.3335535017690674 1103 54-55 0.3240429234444875 1103 56-57 0.3705328630453337 1103 58-59 0.30062649516258233 1103 60-61 0.3382571342363221 1103 62-63 0.337128434575213 1103 64-65 0.30600424364727985 1103 66-67 0.315465352106429 1103 68-69 0.31172177596819495 1103 70-71 0.3485717632728438 1103 72-73 0.37345359448037385 1103 74-75 0.36645358511065496 1103 76-77 0.3400679388525987 1103 78-79 0.413957764478603 1103 80-81 0.3581255434689865 1103 82-83 0.4326867699849828 1103 84-85 0.38380822878857046 1103 86-87 0.3719558671524119 1103 88-89 0.3113942540726775 1103 90-91 0.37559375800809747 1103 92-93 0.36073211378067427 1103 94-95 0.3768169409605342 1103 96-97 0.3347518823996509 1103 98-99 0.3442272292340256 1103 100-101 0.33648424549576106 1104 1 0.3200973514392089 1104 2 0.30930528678413793 1104 3 0.29470773208199574 1104 4 0.21128752681680396 1104 5 0.22981973371268083 1104 6 0.261520248574584 1104 7 0.2480063882524277 1104 8 0.27255546408641607 1104 9 0.3683106764130315 1104 10-11 0.30035553942416726 1104 12-13 0.2443520842880318 1104 14-15 0.33001033627743226 1104 16-17 0.3468065562305114 1104 18-19 0.3283512543594185 1104 20-21 0.3358627717855498 1104 22-23 0.3177129583097553 1104 24-25 0.30997187623156464 1104 26-27 0.32724071782495656 1104 28-29 0.3385439453135888 1104 30-31 0.30140988927218615 1104 32-33 0.33249188970585664 1104 34-35 0.33103131081177395 1104 36-37 0.2823163141306324 1104 38-39 0.3390283779013288 1104 40-41 0.40236599868347156 1104 42-43 0.4084941925624719 1104 44-45 0.44480767517752895 1104 46-47 0.44156223345243717 1104 48-49 0.36680676702098935 1104 50-51 0.41361384673744794 1104 52-53 0.35239995997267926 1104 54-55 0.4103698698205349 1104 56-57 0.31896492204604243 1104 58-59 0.3674742846252457 1104 60-61 0.34136669137606646 1104 62-63 0.3356047713154382 1104 64-65 0.35776148665264884 1104 66-67 0.35215766088421674 1104 68-69 0.3583245075491206 1104 70-71 0.321982954541852 1104 72-73 0.2713471108930321 1104 74-75 0.34295561852147927 1104 76-77 0.2963562785463836 1104 78-79 0.38852127702524086 1104 80-81 0.4039756171059139 1104 82-83 0.3642162900946033 1104 84-85 0.29127943524958155 1104 86-87 0.23019619979411132 1104 88-89 0.2626989973857867 1104 90-91 0.2852693836575746 1104 92-93 0.23304191695845233 1104 94-95 0.23276300658751126 1104 96-97 0.22888708335668184 1104 98-99 0.27724806150395054 1104 100-101 0.27078466838175785 1105 1 0.2849972640432199 1105 2 0.2834968345997311 1105 3 0.25279468291358853 1105 4 0.21502514253342753 1105 5 0.22306371378051892 1105 6 0.2225185777386116 1105 7 0.2421881333997078 1105 8 0.2592682106534099 1105 9 0.345407563274982 1105 10-11 0.2654641032789513 1105 12-13 0.2334567974010966 1105 14-15 0.3107243656064824 1105 16-17 0.2734838382513587 1105 18-19 0.32302709827803255 1105 20-21 0.29277842741470295 1105 22-23 0.21866138560500303 1105 24-25 0.25150534852673445 1105 26-27 0.300807700039158 1105 28-29 0.2781410549157002 1105 30-31 0.277059122757926 1105 32-33 0.33842366599251505 1105 34-35 0.3300435252900087 1105 36-37 0.24127625703925304 1105 38-39 0.32852916199644255 1105 40-41 0.3777776730902431 1105 42-43 0.3874152977735079 1105 44-45 0.38393283558777114 1105 46-47 0.3986176803039143 1105 48-49 0.3871774670285575 1105 50-51 0.30981371390191015 1105 52-53 0.351867640041867 1105 54-55 0.38696776066954897 1105 56-57 0.3739474204493689 1105 58-59 0.39562271129664595 1105 60-61 0.3952395483876572 1105 62-63 0.4252804270317512 1105 64-65 0.4229336847836578 1105 66-67 0.3890834736855737 1105 68-69 0.2931700871517293 1105 70-71 0.30819316558758203 1105 72-73 0.3696951537777622 1105 74-75 0.3742432655478254 1105 76-77 0.41564879233030894 1105 78-79 0.5093875032214825 1105 80-81 0.45909132822349363 1105 82-83 0.49271376261466315 1105 84-85 0.3803819699058728 1105 86-87 0.4252138064699409 1105 88-89 0.42751214336749044 1105 90-91 0.438140507825441 1105 92-93 0.39006354048955316 1105 94-95 0.43107666425491686 1105 96-97 0.4211234605274399 1105 98-99 0.40847182925371683 1105 100-101 0.5251018260477309 1106 1 0.3025461211035676 1106 2 0.2670603323316598 1106 3 0.2557830510876471 1106 4 0.20682337103620085 1106 5 0.22608685666450157 1106 6 0.26163816750253943 1106 7 0.2850602962135156 1106 8 0.28085213148087007 1106 9 0.3613665561336106 1106 10-11 0.3286183783825791 1106 12-13 0.2646808450080371 1106 14-15 0.3555349370785663 1106 16-17 0.3661623083587955 1106 18-19 0.34132614990183185 1106 20-21 0.3023532812580143 1106 22-23 0.29859677582729205 1106 24-25 0.2997115832382349 1106 26-27 0.3093137061039073 1106 28-29 0.3979157312391379 1106 30-31 0.4094879943223404 1106 32-33 0.38649592536766164 1106 34-35 0.37964744454438915 1106 36-37 0.4180006321034426 1106 38-39 0.4130996673455911 1106 40-41 0.4176195223225818 1106 42-43 0.3905025603225809 1106 44-45 0.3604230872411236 1106 46-47 0.36018355017650805 1106 48-49 0.40496776608080154 1106 50-51 0.44062366048868995 1106 52-53 0.45057778413818284 1106 54-55 0.3717850323707381 1106 56-57 0.31295996513627955 1106 58-59 0.41144992775924294 1106 60-61 0.4626192652760537 1106 62-63 0.450564488460067 1106 64-65 0.48690300525657904 1106 66-67 0.5444844209592645 1106 68-69 0.55278410806757 1106 70-71 0.5468655990437554 1106 72-73 0.5053522242394308 1106 74-75 0.47651412308167806 1106 76-77 0.43572116654697624 1106 78-79 0.5106702634730809 1106 80-81 0.5363148318396398 1106 82-83 0.47490390485735645 1106 84-85 0.451632064554115 1106 86-87 0.4591791463366697 1106 88-89 0.45463462926549525 1106 90-91 0.44057906642552425 1106 92-93 0.43216064167701873 1106 94-95 0.5327614586692242 1106 96-97 0.5221986270175698 1106 98-99 0.5803050519741895 1106 100-101 0.6056374607612867 1107 1 0.24321078004041397 1107 2 0.22277875583072415 1107 3 0.20177595785244762 1107 4 0.19237248018238517 1107 5 0.2029803926724938 1107 6 0.1664391032270487 1107 7 0.2168216850461775 1107 8 0.2038985252763439 1107 9 0.24607540178372034 1107 10-11 0.21799794978737452 1107 12-13 0.20842498714788604 1107 14-15 0.20873202016537817 1107 16-17 0.2717007255411019 1107 18-19 0.1998937464834114 1107 20-21 0.20244523038413575 1107 22-23 0.1952689769265703 1107 24-25 0.2193960247863984 1107 26-27 0.2724823719334779 1107 28-29 0.25482025110616036 1107 30-31 0.2555556375372632 1107 32-33 0.28060655290280323 1107 34-35 0.22777354802769878 1107 36-37 0.26085117671691904 1107 38-39 0.29588344827856616 1107 40-41 0.3200516454893929 1107 42-43 0.3293128893946502 1107 44-45 0.2630840417004521 1107 46-47 0.33503937573789244 1107 48-49 0.2803383264508099 1107 50-51 0.3167888261458529 1107 52-53 0.32912417192910937 1107 54-55 0.3745790469438006 1107 56-57 0.3988294552939138 1107 58-59 0.408956987187274 1107 60-61 0.47926097177064264 1107 62-63 0.49301230483536784 1107 64-65 0.5393443121010009 1107 66-67 0.5369442609424482 1107 68-69 0.5844518114548194 1107 70-71 0.5353536032868718 1107 72-73 0.5969879393573692 1107 74-75 0.5372777668394093 1107 76-77 0.5205113756887307 1107 78-79 0.5778395411751163 1107 80-81 0.5694406590707999 1107 82-83 0.5840149263703296 1107 84-85 0.5651839027211629 1107 86-87 0.522778409135281 1107 88-89 0.5264953637294134 1107 90-91 0.6755617538170853 1107 92-93 0.6571299569373075 1107 94-95 0.7287433293241463 1107 96-97 0.704472598268012 1107 98-99 0.7579975566450763 1107 100-101 0.8471054538870106 1108 1 0.1386465300776365 1108 2 0.11993595289970216 1108 3 0.08622837888690782 1108 4 0.09590514874612666 1108 5 0.08937916963646586 1108 6 0.10239802508613849 1108 7 0.13649284520498384 1108 8 0.10825229376890633 1108 9 0.189129311502235 1108 10-11 0.0754716759161127 1108 12-13 0.05283667189238628 1108 14-15 0.09852244455140635 1108 16-17 0.10949727872762338 1108 18-19 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-1.1817502185437618 2316 52-53 -1.2239840632835595 2316 54-55 -1.8765281250545982 2316 56-57 -1.3156023036581033 2316 58-59 -0.9433280036888547 2316 60-61 -0.8435362806858748 2316 62-63 -1.3911281680229948 2316 64-65 -1.665051406359865 2316 66-67 -1.1957470211950607 2316 68-69 -1.17762752905562 2316 70-71 -1.0602949129110755 2316 72-73 -1.1796390949671505 2316 74-75 -1.0099985452411815 2316 76-77 -0.9447138477098953 2316 78-79 -0.9290711636617672 2316 80-81 -0.8279085499472352 2316 82-83 -0.9537943923318579 2316 84-85 -1.1023091319890774 2316 86-87 -1.2198127726150183 2316 88-89 -0.9663212145009048 2316 90-91 -0.9187077487469928 2316 92-93 -0.9138254375151895 2316 94-95 -1.138545939134982 2316 96-97 -1.189563783440743 2316 98-99 -1.1902936849419312 2316 100-101 -1.5240308155660998 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 26.0 4 93.0 5 222.0 6 576.0 7 1620.0 8 4390.0 9 9125.0 10 15251.0 11 23216.0 12 33414.0 13 46443.0 14 58215.0 15 72264.0 16 88801.0 17 113628.0 18 97219.0 19 58717.0 20 46743.0 21 46133.0 22 47141.0 23 51284.0 24 60996.0 25 75979.0 26 95008.0 27 121632.0 28 155408.0 29 197221.0 30 250760.0 31 317554.0 32 407975.0 33 538199.0 34 748065.0 35 1111225.0 36 1742702.0 37 2772125.0 38 3156281.0 39 777728.0 40 306.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.46323934301149 22.468211684567777 16.16778796032924 22.900761012091497 2 14.377501895696309 22.24771800732378 39.6964202928463 23.678359804133613 3 16.639735029259583 27.012045119248373 32.007248085270746 24.340971766221298 4 11.971997177684825 18.660449479546646 39.02977107846388 30.337782264304646 5 12.238361945114482 38.0867291228027 35.86683482049244 13.808074111590368 6 28.894505634344625 39.586199443576476 17.867373411325257 13.651921510753642 7 25.204281964305373 33.91577846276243 23.27211762804124 17.607821944890954 8 24.704630031470305 35.93369120038694 20.9552545776509 18.406424190491848 9 24.505914072736 16.912814387574162 22.527247166655137 36.0540243730347 10-11 22.994165104192 27.712187856980712 29.420617980807844 19.87302905801944 12-13 24.03850704398563 25.307758795478474 29.699644142622184 20.954090017913714 14-15 21.233020668128663 27.258382990363366 27.746650316771415 23.761946024736556 16-17 19.932265357803228 30.50890574563001 28.478874919821646 21.07995397674511 18-19 19.84417119068677 28.634020528742386 31.644089376683638 19.877718903887207 20-21 20.972138744958265 27.770975942060698 31.470493014639207 19.78639229834183 22-23 20.54111497952328 27.749196642697534 31.6057051389612 20.10398323881799 24-25 20.467540191454308 27.77714097651972 31.45265541690525 20.302663415120723 26-27 20.189917838374786 27.950489199514834 31.436996710814398 20.42259625129598 28-29 20.21476865590877 27.899273426930034 31.651875236647392 20.234082680513808 30-31 20.517137596593642 28.251853632712425 31.200564048728342 20.030444721965583 32-33 20.189110876618468 28.015290995573665 31.61552586461813 20.180072263189732 34-35 20.41727711978252 27.90644260751395 31.38824383468585 20.288036438017688 36-37 20.76508410471384 28.015368878029484 30.93241892101435 20.287128096242327 38-39 20.50125015574252 28.196318949438247 31.075748477470498 20.226682417348734 40-41 20.357476515394627 28.042210611340927 31.137117599967983 20.46319527329646 42-43 20.38792055700314 28.110445255302412 30.919431472128387 20.582202715566055 44-45 20.568504513178915 28.328980930766146 30.643302236250097 20.459212319804838 46-47 20.761901471581663 28.58385989030548 30.15198969513629 20.502248942976568 48-49 20.79105419046009 28.431987341765986 29.993600960833888 20.783357506940035 50-51 20.78689468378651 28.577593691490712 29.70555478655864 20.929956838164138 52-53 20.72216756764899 28.763862940769442 29.610408862089788 20.90356062949178 54-55 20.85613612359922 29.071653114955144 29.49752836176861 20.574682399677027 56-57 20.781513467819174 28.76927319790822 29.437302059554877 21.011911274717733 58-59 20.65646811037631 28.530373539710606 29.766191821869796 21.04696652804328 60-61 20.878177253395506 28.721840823697903 29.53003379412184 20.869948128784753 62-63 20.674556577093938 28.745788578914173 29.600211821210877 20.979443022781012 64-65 20.694613528431844 28.674993861266074 29.648411766299514 20.981980844002564 66-67 20.592693808528832 28.69865633471355 29.56147194957395 21.14717790718367 68-69 20.921757291867422 28.923740105558448 29.222927175178885 20.931575427395245 70-71 20.90690493766558 28.627053925738288 29.212424166345034 21.253616970251098 72-73 20.698794891789067 28.815898090584895 29.389243469629484 21.09606354799655 74-75 20.79643651646559 28.951943477277307 29.164811638076838 21.086808368180264 76-77 20.792471584399454 29.038975282083822 29.07516540832471 21.093387725192013 78-79 20.869085350044664 28.933039099630182 29.293289400875484 20.90458614944967 80-81 20.791820804669698 28.932244628533223 29.198635266954952 21.077299299842124 82-83 20.625668997035223 28.88599829627069 29.450966596968197 21.037366109725895 84-85 20.59065802247885 28.95088469128237 29.18696024490225 21.27149704133653 86-87 20.683373324834022 29.03412178788145 29.104008392088353 21.178496495196168 88-89 20.518064939706093 28.941356652920614 29.237942670528717 21.30263573684458 90-91 20.404544298895363 28.86944293049835 29.237437687508912 21.488575083097373 92-93 20.490286842567436 28.930496939056198 29.152804453434634 21.426411764941726 94-95 20.329866846917515 28.796916640315136 29.372120090441676 21.50109642232567 96-97 20.34791594566226 28.931876530971202 29.213103846967254 21.507103676399282 98-99 20.24206641823066 28.85396347562753 29.199866857624478 21.704103248517335 100-101 20.182062103742904 28.974166325900068 29.03105839138856 21.81271317896847 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 7611.0 1 5198.0 2 3685.0 3 4637.5 4 3785.5 5 3273.0 6 5660.5 7 11571.0 8 17700.0 9 20311.5 10 18011.0 11 14938.5 12 15141.5 13 16399.5 14 17647.0 15 18686.0 16 19912.0 17 21593.5 18 23543.0 19 26085.5 20 30160.5 21 35911.0 22 46172.0 23 63063.5 24 81331.5 25 99614.5 26 126522.0 27 162919.0 28 211080.0 29 256627.5 30 283613.5 31 317323.5 32 359174.0 33 393564.5 34 425088.5 35 452978.0 36 467725.5 37 475281.5 38 483990.5 39 488716.5 40 494338.5 41 488703.5 42 467922.5 43 450802.5 44 438269.0 45 425236.5 46 409519.0 47 391955.0 48 378147.5 49 371103.5 50 365182.0 51 348474.0 52 319705.0 53 295996.5 54 282378.5 55 267972.0 56 241112.5 57 211187.0 58 189979.5 59 170367.0 60 149016.0 61 127922.5 62 108422.0 63 88846.0 64 71361.0 65 58479.0 66 47768.5 67 37966.0 68 29324.0 69 21883.5 70 16219.5 71 11777.5 72 8694.5 73 6649.0 74 5118.0 75 3854.5 76 2821.0 77 2058.0 78 1526.5 79 1099.5 80 744.0 81 458.0 82 266.0 83 184.5 84 136.5 85 80.5 86 61.0 87 48.0 88 32.0 89 28.0 90 16.5 91 8.5 92 7.5 93 7.0 94 5.5 95 2.5 96 0.5 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05030842670269069 2 0.002300713093657983 3 2.023428453705718E-4 4 2.548021015777571E-4 5 0.0026304569898174335 6 0.0019185099412913475 7 0.0 8 0.0 9 5.995343566535461E-5 10-11 4.496507674901596E-5 12-13 3.747089729084663E-6 14-15 5.733047285499535E-4 16-17 0.0 18-19 5.620634593626995E-5 20-21 5.170983826136835E-4 22-23 0.0 24-25 6.55740702589816E-4 26-27 0.0076777868548944745 28-29 0.007891370969452301 30-31 0.0012065628927652615 32-33 0.0070033107036592355 34-35 0.008550858761771201 36-37 0.004646391264064982 38-39 0.002634204079546518 40-41 0.00200094591533121 42-43 0.0015700305964864737 44-45 7.56912125275102E-4 46-47 6.519936128607314E-4 48-49 0.0020946231585583265 50-51 0.0014089057381358333 52-53 0.0021733120428691047 54-55 8.243597403986259E-4 56-57 2.173312042869105E-4 58-59 7.56912125275102E-4 60-61 0.006265134027029556 62-63 0.002701651694670042 64-65 0.001045438034414621 66-67 0.0055869107860652325 68-69 0.007745234470017999 70-71 0.0025405268363194017 72-73 5.545692799045302E-4 74-75 0.0033986103842797897 76-77 0.002027175543434803 78-79 0.0024543437725504545 80-81 9.517607911875045E-4 82-83 1.4988358916338652E-4 84-85 5.957872669244614E-4 86-87 0.0048374928402483 88-89 0.0025667564644229943 90-91 0.0011728390852034996 92-93 7.868888431077793E-5 94-95 4.683862161355829E-4 96-97 2.622962810359264E-4 98-99 5.882930874662921E-4 100-101 3.747089729084663E-6 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.3343689E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.00073689255455 #Duplication Level Percentage of deduplicated Percentage of total 1 65.6600747292905 30.204118219675617 2 18.424056045829452 16.950403093157597 3 6.936585659538691 9.57264155571319 4 3.1422819191452005 5.781891352193189 5 1.658465262189937 3.81453120857204 6 0.9808347499303295 2.7071472759971775 7 0.6365411435070499 2.049695316462753 8 0.43577396111980204 1.6036738664078647 9 0.31994413809316546 1.324589950506496 >10 1.6348005932571037 13.590505760371604 >50 0.10849423632413291 3.4259700843532865 >100 0.056044601556507444 4.657030641553246 >500 0.0036498839838475863 1.1637096081283984 >1k 0.0023386028984835536 2.011671739910079 >5k 4.906000104205892E-5 0.14908395696435056 >10k+ 6.541333472274523E-5 0.9933363700330322 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 49925 0.3741469094491036 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 42443 0.31807545874308074 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28702 0.215097938808376 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.4988358916338652E-5 0.0 5 0.0 1.4988358916338652E-5 0.0 1.4988358916338652E-5 0.0 6 0.0 1.4988358916338652E-5 0.0 1.4988358916338652E-5 0.0 7 0.0 1.4988358916338652E-5 0.0 1.4988358916338652E-5 0.0 8 0.0 1.4988358916338652E-5 0.0 1.4988358916338652E-5 0.0 9 0.0 1.4988358916338652E-5 0.0 2.248253837450798E-5 0.0 10-11 0.0 1.4988358916338652E-5 0.0 2.248253837450798E-5 0.0 12-13 0.0 1.4988358916338652E-5 0.0 3.747089729084663E-5 0.0 14-15 0.0 1.4988358916338652E-5 0.0 7.494179458169326E-5 0.0 16-17 0.0 1.4988358916338652E-5 0.0 1.0491851241437057E-4 0.0 18-19 0.0 1.4988358916338652E-5 0.0 1.1990687133070922E-4 0.0 20-21 0.0 1.4988358916338652E-5 0.0 1.6487194807972516E-4 0.0 22-23 0.0 1.4988358916338652E-5 0.0 2.4356083239050313E-4 0.0 24-25 0.0 2.248253837450798E-5 0.0 3.934444215538896E-4 0.0 26-27 0.0 2.9976717832677304E-5 0.0 7.269354074424246E-4 0.0 28-29 0.0 4.496507674901596E-5 0.0 0.0016487194807972517 0.0 30-31 0.0 4.496507674901596E-5 0.0 0.003915708766893473 0.0 32-33 0.0 4.496507674901596E-5 0.0 0.009157887297882916 0.0 34-35 0.0 4.871216647810062E-5 0.0 0.018105937570937092 0.0 36-37 0.0 5.620634593626994E-5 0.0 0.03191396322261408 0.0 38-39 0.0 5.995343566535461E-5 0.0 0.05115901607119291 0.0 40-41 0.0 5.995343566535461E-5 0.0 0.0708312371488874 0.0 42-43 0.0 5.995343566535461E-5 0.0 0.09107676295513183 0.0 44-45 0.0 5.995343566535461E-5 0.0 0.11777852436458913 0.0 46-47 0.0 5.995343566535461E-5 0.0 0.149816141548263 0.0 48-49 0.0 6.744761512352394E-5 0.0 0.18394088771103703 0.0 50-51 0.0 6.744761512352394E-5 0.0 0.2189087290628551 0.0 52-53 0.0 6.744761512352394E-5 0.0 0.2534081841985376 0.0 54-55 0.0 6.744761512352394E-5 0.0 0.29390298290075556 0.0 56-57 0.0 7.119470485260861E-5 0.0 0.34402780220672113 0.0 58-59 0.0 7.494179458169326E-5 0.0 0.40028286030946914 0.0 60-61 0.0 7.494179458169326E-5 0.0 0.4613754112524655 0.0 62-63 3.747089729084663E-6 7.494179458169326E-5 0.0 0.5225953632462508 0.0 64-65 7.494179458169326E-6 7.494179458169326E-5 0.0 0.5899080831395276 0.0 66-67 7.494179458169326E-6 7.868888431077793E-5 0.0 0.6699833906500668 0.0 68-69 7.494179458169326E-6 9.742433295620125E-5 0.0 0.7640241015809046 0.0 70-71 7.494179458169326E-6 1.0491851241437057E-4 0.0 0.8670540807718166 0.0 72-73 7.494179458169326E-6 1.0491851241437057E-4 0.0 0.976480342130276 0.0 74-75 7.494179458169326E-6 1.0491851241437057E-4 0.0 1.0921492549773903 0.0 76-77 7.494179458169326E-6 1.124126918725399E-4 0.0 1.2219859140901739 0.0 78-79 7.494179458169326E-6 1.1990687133070922E-4 0.0 1.3733870745938397 0.0 80-81 7.494179458169326E-6 1.2740105078887855E-4 0.0 1.5427105652717177 0.0 82-83 7.494179458169326E-6 1.2740105078887855E-4 0.0 1.721978832090586 0.0 84-85 7.494179458169326E-6 1.2740105078887855E-4 0.0 1.9073548551678625 0.0 86-87 7.494179458169326E-6 1.2740105078887855E-4 0.0 2.10787286784037 0.0 88-89 7.494179458169326E-6 1.2740105078887855E-4 0.0 2.3362317571999767 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 24195 0.0 57.49181 1 GTATCAA 46655 0.0 42.802265 1 ATCAACG 59545 0.0 33.335835 3 TCAACGC 60430 0.0 32.902527 4 TATCAAC 61330 0.0 32.4514 2 CAACGCA 62775 0.0 31.711264 5 AACGCAG 67590 0.0 29.612507 6 GTGGTAT 10695 0.0 29.50504 1 TGGTATC 10625 0.0 28.924625 2 CGCAGAG 75475 0.0 26.272818 8 ACGCAGA 77225 0.0 25.825058 7 GCAGAGT 81285 0.0 23.99768 9 GTACATG 42080 0.0 23.321327 1 TACATGG 41270 0.0 22.547188 2 AGAGTAC 76515 0.0 21.164064 10-11 ACATGGG 42150 0.0 21.151976 3 GAGTACT 57410 0.0 20.542067 12-13 CAGAGTA 79950 0.0 20.192387 10-11 AGTACTT 58855 0.0 20.05386 12-13 GTACTTT 60995 0.0 19.132387 14-15 >>END_MODULE