FastQCFastQC Report
Wed 25 May 2016
SRR2049412_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049412_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12555090
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT449150.3577433534924879No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT372000.29629417232373484No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210110.16735045308317184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA228200.056.5783581
GTATCAA433350.042.049061
ATCAACG524150.034.382373
TCAACGC528500.034.1554764
CAACGCA548450.032.9126665
TATCAAC549200.032.8993032
AACGCAG576450.031.4296136
GTGGTAT98100.028.7145611
ACGCAGA648650.027.5953587
CGCAGAG655050.027.1443378
TGGTATC105150.025.7479292
GCAGAGT707950.024.5590959
GTACATG401950.023.8132971
TACATGG399500.022.4590782
GAGTACT465950.021.4145312-13
CAGAGTA681800.021.21246110-11
ACATGGG411200.020.9891993
AGAGTAC649350.020.42896810-11
GTACTTT502800.019.50835214-15
AGTACTT482950.019.21482512-13
Produced by FastQC (version 0.11.5)