##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049412_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12555090 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.54252370950746 31.0 31.0 34.0 30.0 34.0 2 31.584140774777403 33.0 31.0 34.0 30.0 34.0 3 31.611462602020374 33.0 31.0 34.0 28.0 34.0 4 35.222974984647664 37.0 35.0 37.0 33.0 37.0 5 35.24256727749462 37.0 35.0 37.0 33.0 37.0 6 35.329557016317686 37.0 35.0 37.0 33.0 37.0 7 35.275107625672135 37.0 35.0 37.0 33.0 37.0 8 35.24884751921332 37.0 35.0 37.0 33.0 37.0 9 36.848797818255385 39.0 37.0 39.0 33.0 39.0 10-11 36.792856283786094 39.0 37.0 39.0 33.0 39.0 12-13 36.75478359772809 39.0 37.0 39.0 33.0 39.0 14-15 37.94844979207636 40.0 38.0 41.0 33.0 41.0 16-17 37.933069496116715 40.0 38.0 41.0 33.0 41.0 18-19 37.95054623264349 40.0 38.0 41.0 33.0 41.0 20-21 37.91152636102171 40.0 38.0 41.0 33.0 41.0 22-23 37.80745458614793 40.0 38.0 41.0 33.0 41.0 24-25 37.6999501795686 40.0 38.0 41.0 32.0 41.0 26-27 37.55883976140354 40.0 37.5 41.0 32.0 41.0 28-29 37.40254661655153 40.0 37.0 41.0 32.0 41.0 30-31 37.221456277892074 40.0 37.0 41.0 31.5 41.0 32-33 36.98243923380876 40.0 36.5 41.0 31.0 41.0 34-35 36.786229887639195 40.0 36.0 41.0 30.5 41.0 36-37 36.602024318423844 39.0 36.0 41.0 30.0 41.0 38-39 36.39726493398295 39.0 35.5 41.0 30.0 41.0 40-41 36.1889529664861 39.0 35.0 41.0 30.0 41.0 42-43 35.95773276017934 39.0 35.0 40.0 29.5 41.0 44-45 35.68698284918706 39.0 35.0 40.0 28.5 41.0 46-47 35.34871848788021 38.5 35.0 40.0 27.0 41.0 48-49 35.061157865057126 38.0 35.0 40.0 26.0 41.0 50-51 33.67495000035842 37.0 32.5 39.0 24.0 40.0 52-53 33.96423303218057 37.5 33.5 39.0 25.0 40.0 54-55 35.02199733335245 38.5 35.0 40.0 26.0 41.0 56-57 35.13638384910025 39.0 35.0 40.0 26.0 41.0 58-59 34.98155596654425 39.0 35.0 40.0 26.0 41.0 60-61 34.72074306118076 38.0 34.0 40.0 26.0 41.0 62-63 34.4451866932057 38.0 34.0 40.0 25.5 41.0 64-65 34.16192253500373 37.5 34.0 40.0 25.0 41.0 66-67 33.838688571726685 37.0 34.0 40.0 23.5 41.0 68-69 33.49249734569804 36.0 33.0 39.5 23.0 41.0 70-71 33.15272483112427 36.0 33.0 39.0 22.5 41.0 72-73 32.891349842972055 35.5 33.0 39.0 22.0 40.0 74-75 32.68571670931869 35.0 33.0 38.5 22.0 40.0 76-77 32.345039661205135 35.0 33.0 37.5 20.5 39.5 78-79 31.969755135168285 35.0 32.5 37.0 20.0 39.0 80-81 31.628044323059413 35.0 32.0 36.5 18.5 39.0 82-83 31.287866395222974 35.0 32.0 36.0 17.5 38.5 84-85 30.967689598401925 35.0 32.0 36.0 14.0 37.0 86-87 30.67558978071842 35.0 32.0 35.0 9.5 37.0 88-89 30.387876948711636 35.0 31.0 35.0 6.5 36.5 90-91 30.122036998540032 35.0 31.0 35.0 2.0 36.0 92-93 29.8699471688375 34.0 31.0 35.0 2.0 36.0 94-95 29.61043911274232 34.0 31.0 35.0 2.0 36.0 96-97 29.353284604092842 34.0 31.0 35.0 2.0 35.0 98-99 29.036683209757953 34.0 30.5 35.0 2.0 35.0 100-101 28.497141876322672 34.0 29.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.31069738838687755 1101 2 0.3412714963994681 1101 3 0.3161548341952134 1101 4 0.3257801002580649 1101 5 0.29863841986551876 1101 6 0.353311496667132 1101 7 0.3637353429442456 1101 8 0.3154677460081956 1101 9 0.3735126288367354 1101 10-11 0.3567635318631446 1101 12-13 0.3616649649659536 1101 14-15 0.454396508678677 1101 16-17 0.43117596463005015 1101 18-19 0.3872447058780182 1101 20-21 0.3558591420066506 1101 22-23 0.40083187169801704 1101 24-25 0.3855578134101094 1101 26-27 0.348492172739995 1101 28-29 0.3559262378787764 1101 30-31 0.3052045569445525 1101 32-33 0.2628014367374405 1101 34-35 0.2815769925524805 1101 36-37 0.25635160966684367 1101 38-39 0.15680343234900107 1101 40-41 0.06732133597532197 1101 42-43 0.061163784508259766 1101 44-45 -0.017604848107133364 1101 46-47 -0.08173743259552424 1101 48-49 -0.25590169721768774 1101 50-51 -0.2570537696667827 1101 52-53 -0.13180961967981375 1101 54-55 0.0902797692271804 1101 56-57 0.14365475729280064 1101 58-59 0.12084738932786365 1101 60-61 0.10603431122640217 1101 62-63 0.07768007800638799 1101 64-65 0.04675035091014479 1101 66-67 0.05622902253224282 1101 68-69 -0.013517776182673913 1101 70-71 -0.09205236283099794 1101 72-73 -0.13188498673028448 1101 74-75 -0.1139922934541957 1101 76-77 -0.1476728443831874 1101 78-79 -0.22807265021470613 1101 80-81 -0.2820270492205097 1101 82-83 -0.2883772937450253 1101 84-85 -0.3542153832332531 1101 86-87 -0.4398691192128332 1101 88-89 -0.47954374346524276 1101 90-91 -0.5462328173784599 1101 92-93 -0.5715936712794267 1101 94-95 -0.7021742440539605 1101 96-97 -0.7330127139685594 1101 98-99 -0.8683825939773193 1101 100-101 -0.9838997264997076 1102 1 0.2666732924630395 1102 2 0.28271346160618904 1102 3 0.2832031063772398 1102 4 0.30753279699943903 1102 5 0.2662315823238188 1102 6 0.319573929611245 1102 7 0.3058150676942333 1102 8 0.3608966789036785 1102 9 0.4176235902485175 1102 10-11 0.4240733761230828 1102 12-13 0.3574970712318759 1102 14-15 0.4722386920713859 1102 16-17 0.3991323185355711 1102 18-19 0.37374491457018877 1102 20-21 0.380712028798726 1102 22-23 0.3864703116019683 1102 24-25 0.35888221513048535 1102 26-27 0.39436221099015256 1102 28-29 0.39359651685072095 1102 30-31 0.3683956228453482 1102 32-33 0.3533035752389182 1102 34-35 0.33920333278627623 1102 36-37 0.3474700414970684 1102 38-39 0.31223909502104874 1102 40-41 0.2426312685611336 1102 42-43 0.16354826378745457 1102 44-45 0.22678270361951292 1102 46-47 0.2028671869798231 1102 48-49 0.12885416485392653 1102 50-51 0.025662289540264283 1102 52-53 0.13578426215021722 1102 54-55 0.2756793567594471 1102 56-57 0.2532097872081849 1102 58-59 0.23219247504066232 1102 60-61 0.3177508692744695 1102 62-63 0.2672521567612023 1102 64-65 0.20174590209634857 1102 66-67 0.2016766922215183 1102 68-69 0.20543468106741614 1102 70-71 0.11532571437789585 1102 72-73 0.13506945206437138 1102 74-75 0.139823449566002 1102 76-77 0.1551767323794664 1102 78-79 0.13771320369460938 1102 80-81 0.1031347677020662 1102 82-83 -0.039772303585071 1102 84-85 -0.01975020345475542 1102 86-87 -0.04363169565943181 1102 88-89 -0.11790168366114173 1102 90-91 -0.1599400588595543 1102 92-93 -0.18632164647343075 1102 94-95 -0.27520328959331763 1102 96-97 -0.33969341099811245 1102 98-99 -0.35845602479264116 1102 100-101 -0.4307266758574677 1103 1 0.26224401327088387 1103 2 0.2694609081280568 1103 3 0.27051268069082113 1103 4 0.2671943259143674 1103 5 0.27554035882113936 1103 6 0.29826133865594073 1103 7 0.3012121025603136 1103 8 0.3264690441909863 1103 9 0.37928471517409434 1103 10-11 0.4005679670216793 1103 12-13 0.3539628076507597 1103 14-15 0.4253705488503172 1103 16-17 0.4542678563419571 1103 18-19 0.42139675200738935 1103 20-21 0.42809257043691673 1103 22-23 0.38153010470217197 1103 24-25 0.4506577872419726 1103 26-27 0.47444393278898644 1103 28-29 0.4240097999212651 1103 30-31 0.41624921576534035 1103 32-33 0.2948890114422298 1103 34-35 0.3496622073337008 1103 36-37 0.3431875067882899 1103 38-39 0.31105533226919846 1103 40-41 0.35486066011679185 1103 42-43 0.2731584348806848 1103 44-45 0.20236956615891444 1103 46-47 0.2570622594035683 1103 48-49 0.21041532665778817 1103 50-51 0.1379130306259313 1103 52-53 0.33199705463861306 1103 54-55 0.4383073506665838 1103 56-57 0.5031950090020985 1103 58-59 0.49187321174470355 1103 60-61 0.4265944387048748 1103 62-63 0.4588638200782995 1103 64-65 0.42524195554602784 1103 66-67 0.38979550131842444 1103 68-69 0.36962838906064377 1103 70-71 0.3429461317715905 1103 72-73 0.4377667701095831 1103 74-75 0.39016890791102554 1103 76-77 0.2906539649368298 1103 78-79 0.30228275392113346 1103 80-81 0.2571881988427265 1103 82-83 0.24470262009415578 1103 84-85 0.23914906610751885 1103 86-87 0.1896133257174455 1103 88-89 0.1916182298293343 1103 90-91 0.111087589405642 1103 92-93 0.08991728469222693 1103 94-95 0.09850453373944745 1103 96-97 0.07560612184120075 1103 98-99 0.04560551626032705 1103 100-101 0.01514964092691784 1104 1 0.09501861575571979 1104 2 0.1226603924185028 1104 3 0.2061982309174617 1104 4 0.11128297847668023 1104 5 0.11319102107952972 1104 6 0.09232036600094773 1104 7 0.07276841338626383 1104 8 0.05346658143444927 1104 9 0.08573733293695796 1104 10-11 0.13339749261959355 1104 12-13 0.05859131531200035 1104 14-15 0.0959469728493687 1104 16-17 0.11515054930290347 1104 18-19 0.0550166368485705 1104 20-21 0.08668599782697584 1104 22-23 0.10053121557515254 1104 24-25 0.14514833094489887 1104 26-27 0.0999987973998131 1104 28-29 0.0790561253895774 1104 30-31 0.044693237324203494 1104 32-33 0.011451757515992256 1104 34-35 -0.020788875999365075 1104 36-37 0.00871043060981691 1104 38-39 -0.041023926770002106 1104 40-41 -0.0729642991711259 1104 42-43 -0.06037133829209296 1104 44-45 -0.08616992718402372 1104 46-47 -0.06630269994349902 1104 48-49 -0.0974392055252622 1104 50-51 -0.1693792088410646 1104 52-53 -0.07106447858448206 1104 54-55 0.03990262962955882 1104 56-57 -0.060447081924273505 1104 58-59 0.019640461195251646 1104 60-61 -0.012534934028884948 1104 62-63 -0.01690630097473189 1104 64-65 0.013557216793280702 1104 66-67 0.0344276106154453 1104 68-69 0.006906358224377129 1104 70-71 0.006347833920479218 1104 72-73 -0.07488495186233024 1104 74-75 -0.06982005666076674 1104 76-77 -0.05378670587990797 1104 78-79 -0.09110778439538691 1104 80-81 -0.0811916815487912 1104 82-83 -0.04534645633233225 1104 84-85 -0.04257688633860468 1104 86-87 -0.13040413952414198 1104 88-89 -0.12902764489991014 1104 90-91 -0.19130118433936616 1104 92-93 -0.2007950447542406 1104 94-95 -0.22950547794355813 1104 96-97 -0.27447111907881094 1104 98-99 -0.2284750738852246 1104 100-101 -0.3012491459165787 1105 1 0.26279376480819394 1105 2 0.3132072342954615 1105 3 0.2792597653008819 1105 4 0.29306860736762985 1105 5 0.2947174099005778 1105 6 0.3001866877296564 1105 7 0.29884677115833824 1105 8 0.3103746913197014 1105 9 0.3627704930625697 1105 10-11 0.3722954773611278 1105 12-13 0.2891724080450686 1105 14-15 0.4015050643891698 1105 16-17 0.3598190804202801 1105 18-19 0.365085653230814 1105 20-21 0.4169251271743377 1105 22-23 0.3520322609703328 1105 24-25 0.376949140212993 1105 26-27 0.4103386464852221 1105 28-29 0.42919122413057664 1105 30-31 0.39652889113823875 1105 32-33 0.38099368084539975 1105 34-35 0.4621428597276065 1105 36-37 0.43418025507425995 1105 38-39 0.3652492075777545 1105 40-41 0.41735889952386174 1105 42-43 0.4175590169161154 1105 44-45 0.36463722820698763 1105 46-47 0.3990227263134969 1105 48-49 0.35811641496129454 1105 50-51 0.2888969898087268 1105 52-53 0.3889050959752467 1105 54-55 0.548175751407662 1105 56-57 0.555658398995547 1105 58-59 0.5847141965296245 1105 60-61 0.5863650760697183 1105 62-63 0.590195899912878 1105 64-65 0.5566973714660222 1105 66-67 0.5778277159237248 1105 68-69 0.5812217683604715 1105 70-71 0.5049363504227458 1105 72-73 0.5841439979658887 1105 74-75 0.5414528857730119 1105 76-77 0.5295726488569201 1105 78-79 0.5211441337122196 1105 80-81 0.5224360065378377 1105 82-83 0.5613150713521691 1105 84-85 0.5671800796333777 1105 86-87 0.5032203339880716 1105 88-89 0.5193802935981324 1105 90-91 0.5154162875249639 1105 92-93 0.5098392884564191 1105 94-95 0.43999692139768243 1105 96-97 0.43125546901179135 1105 98-99 0.44515466430576467 1105 100-101 0.45968630601963767 1106 1 0.28734187719214077 1106 2 0.3203963838103441 1106 3 0.3361269418865689 1106 4 0.3053564901178234 1106 5 0.3425688059803278 1106 6 0.391666858888982 1106 7 0.39962119304085064 1106 8 0.403931285466534 1106 9 0.40853672806643715 1106 10-11 0.4464406273960222 1106 12-13 0.5036788148608764 1106 14-15 0.4997662020924736 1106 16-17 0.5406426760649765 1106 18-19 0.5560358596386621 1106 20-21 0.5462012295981253 1106 22-23 0.5821499265246288 1106 24-25 0.5615326117810326 1106 26-27 0.6053649796871738 1106 28-29 0.620851602461272 1106 30-31 0.6554976897527354 1106 32-33 0.6453214162937755 1106 34-35 0.6576699378840019 1106 36-37 0.6229226089999074 1106 38-39 0.6371587785276134 1106 40-41 0.6039411640376926 1106 42-43 0.6378005954800017 1106 44-45 0.5857688721584537 1106 46-47 0.578508693536385 1106 48-49 0.5457961926386403 1106 50-51 0.5246072665886388 1106 52-53 0.6713656801567751 1106 54-55 0.7267426600804754 1106 56-57 0.7557597212986522 1106 58-59 0.7099280203311764 1106 60-61 0.6943196561903093 1106 62-63 0.6441363109174816 1106 64-65 0.6842142249845082 1106 66-67 0.689796969067018 1106 68-69 0.7740718080912998 1106 70-71 0.7669651380456557 1106 72-73 0.7487464749687831 1106 74-75 0.7308722099733345 1106 76-77 0.702179958942807 1106 78-79 0.6688395428724903 1106 80-81 0.6553539157112738 1106 82-83 0.6473787207488897 1106 84-85 0.6462934164807201 1106 86-87 0.6755608050860218 1106 88-89 0.7308543923109418 1106 90-91 0.7000789576678912 1106 92-93 0.6166390174978744 1106 94-95 0.6912749023313829 1106 96-97 0.7031102895082384 1106 98-99 0.7347556345287565 1106 100-101 0.7715922163588758 1107 1 0.18210819093465958 1107 2 0.17781235155812425 1107 3 0.16559328135403462 1107 4 0.1989565998827345 1107 5 0.15293217951965232 1107 6 0.18368780601438317 1107 7 0.2245736867407473 1107 8 0.27199208253875184 1107 9 0.3093636144146288 1107 10-11 0.2517902036672055 1107 12-13 0.2905528684952685 1107 14-15 0.37155788768966147 1107 16-17 0.31061745057637324 1107 18-19 0.279169381268062 1107 20-21 0.33188516701129345 1107 22-23 0.3795200449557896 1107 24-25 0.30314863446911744 1107 26-27 0.32413382986307937 1107 28-29 0.33818315082918105 1107 30-31 0.4232017050619703 1107 32-33 0.3975716500166726 1107 34-35 0.37356473886086405 1107 36-37 0.3888780776528833 1107 38-39 0.42192708431429793 1107 40-41 0.3530036690997562 1107 42-43 0.4063403461138648 1107 44-45 0.4632607472987402 1107 46-47 0.46354337496745046 1107 48-49 0.39541428614630547 1107 50-51 0.4015884273082264 1107 52-53 0.44765554080624526 1107 54-55 0.5278423894671604 1107 56-57 0.4941537861062386 1107 58-59 0.5038716733467794 1107 60-61 0.5627247341287998 1107 62-63 0.5971388570893268 1107 64-65 0.613435912309555 1107 66-67 0.6028360334773524 1107 68-69 0.5832566237973182 1107 70-71 0.599755508373029 1107 72-73 0.5767538542100255 1107 74-75 0.5546758071461326 1107 76-77 0.5731257609748823 1107 78-79 0.5640736639856954 1107 80-81 0.5607922493132946 1107 82-83 0.6141338924465103 1107 84-85 0.5813599479596441 1107 86-87 0.6038942441603226 1107 88-89 0.5840824270831995 1107 90-91 0.6299973180863461 1107 92-93 0.6299457953847565 1107 94-95 0.6502275716127137 1107 96-97 0.6696557349200596 1107 98-99 0.6613011151339983 1107 100-101 0.7183441213354769 1108 1 0.10407211007956363 1108 2 0.12120514137191307 1108 3 0.11816580609712801 1108 4 0.12511362387800062 1108 5 0.16241274730728605 1108 6 0.18907816681296197 1108 7 0.18786539045729 1108 8 0.2504876366891864 1108 9 0.21810902923695608 1108 10-11 0.21535306841168023 1108 12-13 0.21566303313547053 1108 14-15 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-0.5206359858532146 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 104534.0 3 22325.0 4 17797.0 5 16141.0 6 17471.0 7 18384.0 8 20894.0 9 24903.0 10 30352.0 11 36134.0 12 43671.0 13 51859.0 14 57659.0 15 67410.0 16 80943.0 17 100118.0 18 83149.0 19 54356.0 20 49812.0 21 50866.0 22 54103.0 23 59734.0 24 68971.0 25 81854.0 26 98915.0 27 120220.0 28 147113.0 29 181685.0 30 227392.0 31 288430.0 32 374891.0 33 507840.0 34 716550.0 35 1086890.0 36 1702700.0 37 2571178.0 38 2670149.0 39 647428.0 40 269.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.93876825300929 20.208365247148148 14.480169064155909 22.372697435686643 2 15.970623472212742 20.802057395615424 39.86786265624163 23.35945647593021 3 18.349530207415093 26.074103775489792 30.590319089052976 24.986046928042136 4 12.810406402626404 17.379898980018716 38.6578657848261 31.15182883252878 5 12.987139808543596 37.47036172554177 35.262887750830124 14.279610715084504 6 29.858820155804434 37.88744802536203 18.042901977027608 14.21082984180593 7 26.318318993789603 33.06686651412607 22.887931724554914 17.726882767529418 8 25.21890428141966 34.948680855280315 20.87403363905458 18.958381224245443 9 25.259724167959035 16.788017714796933 21.969214259364833 35.983043857879196 10-11 23.711905878589484 26.967091726030752 28.88654986933423 20.434452526045533 12-13 24.741059978720674 24.73660720366603 28.966799264838077 21.555533552775223 14-15 22.092092441316147 26.412508962817434 26.982827711749103 24.51257088411732 16-17 20.96043181412761 29.60714904597755 27.55745137489052 21.87496776500432 18-19 20.87342994461062 27.99493459474886 30.430469439303113 20.701166021337407 20-21 21.892314971676768 27.24401754157007 30.245197989817026 20.618469496936136 22-23 21.607230073752596 27.14701406967197 30.31068308685946 20.93507276971597 24-25 21.327824412302434 27.30397618482924 30.287205080856523 21.080994322011808 26-27 21.108301773419196 27.360804769048976 30.263714185856166 21.267179271675662 28-29 21.16681869782081 27.284166039923957 30.4276326728192 21.121382589436035 30-31 21.39775082133432 27.632165680242437 30.043566212817936 20.92651728560531 32-33 21.0924701868182 27.512086285127925 30.249285280933 21.146158247120873 34-35 21.346996348109464 27.244537915057165 30.1923429376034 21.21612279922997 36-37 21.6840981947096 27.47306974875931 29.72345611083707 21.119375945694017 38-39 21.46999127988757 27.71319511992305 29.79167310506413 21.025140495125243 40-41 21.359951336292536 27.45808832619021 29.919155607891717 21.262804729625536 42-43 21.354031093873907 27.614371796632287 29.654877402635304 21.3767197068585 44-45 21.480658143391576 27.780611063280475 29.43629681809581 21.30243397523214 46-47 21.685592878239945 27.81841829964613 29.12464255080762 21.3713462713063 48-49 21.636338053265323 27.888665165711913 28.9472775537382 21.527719227284564 50-51 21.662548518392594 27.953599651627798 28.700585456631362 21.68326637334825 52-53 21.66329553091475 28.060004211612878 28.631563174730836 21.645137082741545 54-55 21.658137091776133 28.429438131371715 28.571435968008608 21.34098880884354 56-57 21.64219908974568 28.106695790046892 28.590179533420283 21.660925586787137 58-59 21.512408891222588 27.79788300734478 28.852089941998806 21.837618159433827 60-61 21.75936759802487 28.01922130006979 28.626962835145342 21.594448266759994 62-63 21.558408177522796 28.109872200718062 28.66013963021089 21.671579991548256 64-65 21.62869267645413 27.934703377779947 28.75182913116215 21.68477481460377 66-67 21.531286038276935 28.015399058534896 28.621115910827204 21.832198992360965 68-69 21.831090134337057 28.20550133741272 28.402755973434306 21.56065255481592 70-71 21.84234133325368 27.980792226088603 28.454530882082068 21.722335558575644 72-73 21.638345320855844 28.107879857071282 28.529933202731243 21.72384161934163 74-75 21.69593958307729 28.223056142334308 28.41378257863289 21.66722169595551 76-77 21.777390812008395 28.275468489054 28.20640728649056 21.740733412447042 78-79 21.794803986752246 28.252944673286294 28.341997975351667 21.61025336460979 80-81 21.732585597280003 28.21064355679476 28.334775888089244 21.721994957835992 82-83 21.640024003370605 28.20985137140325 28.4815064034878 21.668618221738345 84-85 21.646263702143166 28.213773586198183 28.2927002567616 21.847262454897056 86-87 21.747984778390443 28.331073233873553 28.24512320930645 21.67581877842955 88-89 21.635381281976276 28.18495119464058 28.3227997762594 21.85686774712374 90-91 21.530952285655626 28.142691610975675 28.34498129893684 21.981374804431862 92-93 21.64513089622834 28.258228049947892 28.210398364726558 21.88624268909721 94-95 21.557551805854715 28.05101423325623 28.386388531514818 22.005045429374242 96-97 21.624923451046843 28.13464602931714 28.272461026989955 21.967969492646063 98-99 21.56739828392317 28.145609169360092 28.19332177981897 22.093670766897773 100-101 21.62140353222199 28.081804459722402 28.151640216251078 22.14515179180453 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 7429.0 1 4995.0 2 3607.5 3 4778.0 4 3898.5 5 3254.5 6 5312.0 7 9756.5 8 15380.5 9 18471.0 10 16126.0 11 12967.0 12 12726.5 13 13462.0 14 14181.0 15 14888.0 16 15909.0 17 16966.5 18 18408.0 19 20375.5 20 23035.5 21 27065.0 22 33100.5 23 42980.5 24 55433.0 25 70111.5 26 90342.5 27 115878.5 28 147958.0 29 183467.0 30 214681.5 31 245596.5 32 276752.0 33 307074.5 34 335091.0 35 356838.5 36 374741.0 37 392992.0 38 410979.5 39 421670.5 40 430848.5 41 436964.5 42 436063.0 43 436318.5 44 434600.5 45 430150.5 46 424788.0 47 414307.0 48 404121.0 49 398540.0 50 390590.0 51 374382.0 52 348046.0 53 324503.5 54 308904.5 55 293371.5 56 271380.0 57 243334.5 58 218752.5 59 199009.0 60 177141.5 61 152623.0 62 130249.5 63 108238.5 64 88092.5 65 71816.5 66 57578.0 67 45140.0 68 35228.5 69 27347.0 70 20740.0 71 15910.5 72 12518.5 73 9500.5 74 7124.5 75 5300.5 76 3788.5 77 2818.0 78 2163.5 79 1611.5 80 1178.0 81 873.0 82 682.0 83 579.5 84 490.0 85 405.0 86 347.5 87 292.5 88 247.5 89 211.0 90 176.0 91 151.0 92 139.5 93 115.0 94 87.0 95 71.5 96 54.5 97 44.0 98 34.0 99 24.0 100 12.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013930605037478823 2 0.004595745629860081 3 0.0040382028324767085 4 0.005909953652263743 5 0.003010731105870209 6 0.007088758423874301 7 0.0014416463760912905 8 0.0041098869064259995 9 0.010282682163170475 10-11 0.007343635131249557 12-13 0.008928649655239429 14-15 0.0035364143148316736 16-17 0.006367935235828656 18-19 0.004117851803531476 20-21 9.040158214716103E-4 22-23 0.0011310153889776975 24-25 0.007228144123220145 26-27 0.0010672962121338835 28-29 7.765774677839823E-4 30-31 4.978060690922965E-4 32-33 0.0012504888455598486 34-35 0.0017761720545213138 36-37 0.0012504888455598486 38-39 0.001640768803728209 40-41 0.0028195735753387668 42-43 0.002660275633229232 44-45 0.007618424081388504 46-47 0.013799184235238458 48-49 0.0050696570076359465 50-51 0.005587375319491935 52-53 0.013576167116285107 54-55 0.008168001981666399 56-57 0.005467901862909784 58-59 0.006897600893342859 60-61 0.0013062431252981858 62-63 0.00227397812361361 64-65 0.002313802609140994 66-67 0.002361591991773854 68-69 0.002007154070580139 70-71 0.002210258946769796 72-73 0.001278365985429017 74-75 0.0015690847297789182 76-77 0.008028616282320556 78-79 0.0064595315525416384 80-81 0.012357537859147165 82-83 0.014758954336448405 84-85 0.008219773812851999 86-87 0.005587375319491935 88-89 0.0029669241717900866 90-91 0.0025686793165162498 92-93 9.995945867373312E-4 94-95 0.0012942957796399706 96-97 0.001716435326230238 98-99 0.0060294271088458945 100-101 0.010744646195288126 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.255509E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.773053921490096 #Duplication Level Percentage of deduplicated Percentage of total 1 67.24309935332887 30.779220125480382 2 17.412103448155104 15.940103000379343 3 6.4110647226782405 8.803620337359423 4 2.9252266741752053 5.355862331584112 5 1.6389572998959538 3.751004043157865 6 0.9513961983890863 2.61289856937386 7 0.6460128000418944 2.0698985111203303 8 0.46643745871926856 1.7080213559167914 9 0.3259939702974828 1.3429565622456587 >10 1.773661244427335 14.853740962542657 >50 0.13024287631954046 4.130127023415928 >100 0.07136096191858851 5.856751028827596 >500 0.003257682524225962 0.9943749890346487 >1k 0.0010807261073302933 0.7928622826461219 >5k 5.229151092386061E-5 0.18577078466967709 >10k+ 5.229151092386061E-5 0.8227880922455386 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 44915 0.3577433534924879 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 37200 0.29629417232373484 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21011 0.16735045308317184 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 7.964897105476743E-6 0.0 5 0.0 0.0 0.0 7.964897105476743E-6 0.0 6 0.0 0.0 0.0 7.964897105476743E-6 0.0 7 0.0 0.0 0.0 7.964897105476743E-6 0.0 8 0.0 0.0 0.0 7.964897105476743E-6 0.0 9 0.0 0.0 0.0 3.9824485527383716E-5 0.0 10-11 0.0 0.0 0.0 4.7789382632860454E-5 0.0 12-13 0.0 0.0 0.0 5.57542797383372E-5 0.0 14-15 0.0 0.0 0.0 6.770162539655232E-5 0.0 16-17 0.0 0.0 0.0 7.168407394929069E-5 0.0 18-19 0.0 0.0 0.0 7.964897105476743E-5 0.0 20-21 0.0 0.0 0.0 9.95612138184593E-5 0.0 22-23 0.0 0.0 0.0 1.831926334259651E-4 7.964897105476743E-6 24-25 0.0 0.0 0.0 3.8231506106288363E-4 7.964897105476743E-6 26-27 0.0 3.9824485527383715E-6 0.0 6.65068908307308E-4 7.964897105476743E-6 28-29 0.0 7.964897105476743E-6 0.0 0.0015292602442515345 7.964897105476743E-6 30-31 0.0 7.964897105476743E-6 0.0 0.004078027318004092 7.964897105476743E-6 32-33 3.9824485527383715E-6 7.964897105476743E-6 0.0 0.00960964835775769 7.964897105476743E-6 34-35 1.5929794210953486E-5 7.964897105476743E-6 0.0 0.019163542435777045 7.964897105476743E-6 36-37 1.5929794210953486E-5 7.964897105476743E-6 0.0 0.03531635376568388 7.964897105476743E-6 38-39 1.5929794210953486E-5 7.964897105476743E-6 0.0 0.05637952416111712 7.964897105476743E-6 40-41 1.5929794210953486E-5 1.1947345658215114E-5 0.0 0.07840644710631306 7.964897105476743E-6 42-43 1.5929794210953486E-5 1.5929794210953486E-5 0.0 0.10117012303376559 7.964897105476743E-6 44-45 1.5929794210953486E-5 1.5929794210953486E-5 0.0 0.1295450689720265 7.964897105476743E-6 46-47 1.9912242763691855E-5 1.5929794210953486E-5 0.0 0.16463840561875703 7.964897105476743E-6 48-49 3.185958842190697E-5 1.5929794210953486E-5 0.0 0.20148800207724515 7.964897105476743E-6 50-51 3.9824485527383716E-5 3.185958842190697E-5 0.0 0.23965180655813698 7.964897105476743E-6 52-53 3.9824485527383716E-5 3.185958842190697E-5 0.0 0.27779968124481785 7.964897105476743E-6 54-55 3.9824485527383716E-5 3.185958842190697E-5 0.0 0.32199689528310826 1.1947345658215114E-5 56-57 3.9824485527383716E-5 3.185958842190697E-5 0.0 0.37599093276113515 1.5929794210953486E-5 58-59 3.9824485527383716E-5 3.9824485527383716E-5 0.0 0.4366953960505261 1.5929794210953486E-5 60-61 3.9824485527383716E-5 3.9824485527383716E-5 0.0 0.5008486597865885 1.5929794210953486E-5 62-63 3.9824485527383716E-5 3.9824485527383716E-5 0.0 0.567315726131792 1.5929794210953486E-5 64-65 3.9824485527383716E-5 3.9824485527383716E-5 0.0 0.6399715175279508 1.9912242763691855E-5 66-67 3.9824485527383716E-5 3.9824485527383716E-5 0.0 0.7275893681367478 2.3894691316430227E-5 68-69 3.9824485527383716E-5 3.9824485527383716E-5 0.0 0.8270191611529667 2.3894691316430227E-5 70-71 3.9824485527383716E-5 3.9824485527383716E-5 0.0 0.9371497934303936 2.3894691316430227E-5 72-73 3.9824485527383716E-5 3.9824485527383716E-5 0.0 1.0536005715610162 2.3894691316430227E-5 74-75 4.7789382632860454E-5 3.9824485527383716E-5 0.0 1.1765706179724718 2.3894691316430227E-5 76-77 4.7789382632860454E-5 3.9824485527383716E-5 0.0 1.3137380934744394 2.3894691316430227E-5 78-79 4.7789382632860454E-5 3.9824485527383716E-5 0.0 1.470985074579314 2.3894691316430227E-5 80-81 4.7789382632860454E-5 4.7789382632860454E-5 7.964897105476743E-6 1.647037177750219 2.3894691316430227E-5 82-83 5.57542797383372E-5 4.7789382632860454E-5 7.964897105476743E-6 1.8365021676467475 2.3894691316430227E-5 84-85 5.57542797383372E-5 4.7789382632860454E-5 7.964897105476743E-6 2.0316740063193492 2.3894691316430227E-5 86-87 5.57542797383372E-5 4.7789382632860454E-5 7.964897105476743E-6 2.242958831836331 2.3894691316430227E-5 88-89 5.57542797383372E-5 4.7789382632860454E-5 7.964897105476743E-6 2.482797813476447 3.185958842190697E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 22820 0.0 56.578358 1 GTATCAA 43335 0.0 42.04906 1 ATCAACG 52415 0.0 34.38237 3 TCAACGC 52850 0.0 34.155476 4 CAACGCA 54845 0.0 32.912666 5 TATCAAC 54920 0.0 32.899303 2 AACGCAG 57645 0.0 31.429613 6 GTGGTAT 9810 0.0 28.714561 1 ACGCAGA 64865 0.0 27.595358 7 CGCAGAG 65505 0.0 27.144337 8 TGGTATC 10515 0.0 25.747929 2 GCAGAGT 70795 0.0 24.559095 9 GTACATG 40195 0.0 23.813297 1 TACATGG 39950 0.0 22.459078 2 GAGTACT 46595 0.0 21.41453 12-13 CAGAGTA 68180 0.0 21.212461 10-11 ACATGGG 41120 0.0 20.989199 3 AGAGTAC 64935 0.0 20.428968 10-11 GTACTTT 50280 0.0 19.508352 14-15 AGTACTT 48295 0.0 19.214825 12-13 >>END_MODULE