FastQCFastQC Report
Wed 25 May 2016
SRR2049412_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049412_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12555090
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT425920.3392408975164654No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT357040.28437868625394164No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245050.19517980356970757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA213450.055.167911
GTATCAA398850.040.8901861
ATCAACG509050.031.714483
TCAACGC514900.031.2986854
TATCAAC531900.030.4418322
CAACGCA537600.030.0301175
AACGCAG566150.028.6078056
GTGGTAT97950.026.1011241
ACGCAGA651000.024.8130657
CGCAGAG657500.024.4305138
GTACATG374050.024.0747011
TGGTATC104350.023.4871862
TACATGG373350.022.6707212
GCAGAGT713700.021.9544779
ACATGGG385600.020.7553353
AGAGTAC654950.020.16729410-11
GAGTACT486650.019.73914312-13
CAGAGTA687400.019.28089710-11
AGTACTT503650.018.75695812-13
GTACTTT520100.018.21864114-15
Produced by FastQC (version 0.11.5)