##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049412_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12555090 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.05086765606619 33.0 31.0 34.0 30.0 34.0 2 32.24827866626205 34.0 31.0 34.0 30.0 34.0 3 32.321576667311824 34.0 31.0 34.0 30.0 34.0 4 35.959791606432134 37.0 35.0 37.0 35.0 37.0 5 35.84055797290183 37.0 35.0 37.0 35.0 37.0 6 35.86376170939436 37.0 35.0 37.0 35.0 37.0 7 35.78892066882834 37.0 35.0 37.0 35.0 37.0 8 35.78110017530739 37.0 35.0 37.0 33.0 37.0 9 37.39237926609845 39.0 37.0 39.0 34.0 39.0 10-11 37.38598436968592 39.0 37.0 39.0 34.0 39.0 12-13 37.365075718294335 39.0 37.0 39.0 34.0 39.0 14-15 38.596127626325256 40.0 38.0 41.0 34.0 41.0 16-17 38.58055904019804 40.0 38.0 41.0 34.0 41.0 18-19 38.606158697388864 40.0 38.0 41.0 34.0 41.0 20-21 38.58977319159003 40.0 38.0 41.0 34.0 41.0 22-23 38.503761621780484 40.0 38.0 41.0 34.0 41.0 24-25 38.4016595659609 40.0 38.0 41.0 34.0 41.0 26-27 38.221991638451016 40.0 38.0 41.0 34.0 41.0 28-29 38.0343763366093 40.0 37.5 41.0 33.0 41.0 30-31 37.85312036791453 40.0 37.0 41.0 33.0 41.0 32-33 37.60982677145286 40.0 37.0 41.0 33.0 41.0 34-35 37.42484370880655 40.0 36.5 41.0 32.0 41.0 36-37 37.23491046260919 39.0 36.0 41.0 31.5 41.0 38-39 37.39422791871663 40.0 37.0 41.0 32.5 41.0 40-41 37.308213561193114 40.0 37.0 41.0 32.0 41.0 42-43 37.12252460954083 40.0 36.0 41.0 31.5 41.0 44-45 36.90050421781126 40.0 36.0 41.0 31.0 41.0 46-47 36.59581588025255 39.0 36.0 41.0 30.5 41.0 48-49 36.337887024306475 39.0 35.5 41.0 30.0 41.0 50-51 36.11187952455936 39.0 35.0 41.0 29.5 41.0 52-53 35.92681334821176 39.0 35.0 40.0 29.0 41.0 54-55 35.811747028496015 39.0 35.0 40.0 29.0 41.0 56-57 35.716185865652896 39.0 35.0 40.0 28.5 41.0 58-59 35.475475285322524 38.5 35.0 40.0 28.0 41.0 60-61 35.227838430469234 38.0 34.5 40.0 28.0 41.0 62-63 34.94188197774767 38.0 34.0 40.0 27.5 41.0 64-65 34.67662943873759 37.0 34.0 40.0 27.0 41.0 66-67 34.340617709630116 37.0 34.0 40.0 26.0 41.0 68-69 34.01865733340024 36.0 34.0 39.0 26.0 41.0 70-71 33.68338371130753 36.0 33.0 39.0 26.0 40.5 72-73 33.31589594339826 35.0 33.0 39.0 25.5 40.0 74-75 32.94359693956794 35.0 33.0 38.0 25.0 40.0 76-77 30.921306617475462 33.5 30.0 35.5 22.0 38.5 78-79 32.24913278200316 35.0 32.0 37.0 24.5 39.0 80-81 32.26835287520838 35.0 33.0 36.5 25.0 39.0 82-83 32.00312936824825 35.0 33.0 36.0 24.0 38.0 84-85 31.7411876378425 35.0 32.0 36.0 24.0 37.0 86-87 31.47950397010296 35.0 32.0 35.0 23.5 37.0 88-89 31.218622009081578 35.0 32.0 35.0 22.5 36.5 90-91 30.958508859753294 35.0 32.0 35.0 20.0 36.0 92-93 30.718319024395683 34.5 32.0 35.0 19.5 36.0 94-95 30.462840409746164 34.0 31.5 35.0 18.0 35.5 96-97 30.222422260613026 34.0 31.0 35.0 11.5 35.0 98-99 29.933347869270552 34.0 31.0 35.0 2.0 35.0 100-101 29.31287119407348 34.0 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.36960615151540566 1101 2 0.39060551242949515 1101 3 0.3584465458065367 1101 4 0.27754181994330196 1101 5 0.30084881519825757 1101 6 0.3352419924228087 1101 7 0.3148863002526383 1101 8 0.35455273720206293 1101 9 0.3802100942598656 1101 10-11 0.37075279201373945 1101 12-13 0.3184444575083347 1101 14-15 0.40885443944388555 1101 16-17 0.370993225963808 1101 18-19 0.34408323776157346 1101 20-21 0.34244243004335573 1101 22-23 0.3040943192463743 1101 24-25 0.29563287905912006 1101 26-27 0.2535320397036074 1101 28-29 0.2780839634612633 1101 30-31 0.24507043169739262 1101 32-33 0.2722758936269045 1101 34-35 0.1979041017460048 1101 36-37 0.19110013793348202 1101 38-39 0.37039046750346927 1101 40-41 0.35890155082418573 1101 42-43 0.40366060036290463 1101 44-45 0.30833875733055294 1101 46-47 0.2755263959734009 1101 48-49 0.20687231135521955 1101 50-51 0.2549512464662911 1101 52-53 0.2159192573494053 1101 54-55 0.1913831317739536 1101 56-57 0.15465765636130158 1101 58-59 0.10287927162369215 1101 60-61 0.11134314861275385 1101 62-63 0.09188673712169759 1101 64-65 0.02530562299484984 1101 66-67 -0.00796875598908997 1101 68-69 -0.03784680437632204 1101 70-71 -0.06822926252401373 1101 72-73 -0.10935164616507365 1101 74-75 -0.1490337552048473 1101 76-77 -0.170251270659751 1101 78-79 -0.07645131132432681 1101 80-81 -0.08868513582392268 1101 82-83 -0.10823994479730459 1101 84-85 -0.13456696097060927 1101 86-87 -0.13042299843340643 1101 88-89 -0.22592849019020278 1101 90-91 -0.21064719517932673 1101 92-93 -0.2661520005478941 1101 94-95 -0.3492942954438618 1101 96-97 -0.39766634975983095 1101 98-99 -0.5074727507878549 1101 100-101 -0.5494529434566751 1102 1 0.38231178415949785 1102 2 0.33142209750673857 1102 3 0.33726758641803656 1102 4 0.28812534656609756 1102 5 0.24984758430185394 1102 6 0.2906699840501332 1102 7 0.2907431642643843 1102 8 0.27945947004948124 1102 9 0.423413470882565 1102 10-11 0.3536728350229694 1102 12-13 0.31605574786365054 1102 14-15 0.3893566396058006 1102 16-17 0.394799619585541 1102 18-19 0.3722205601715771 1102 20-21 0.35146212772577456 1102 22-23 0.33931983639993746 1102 24-25 0.35059311206848776 1102 26-27 0.3193243611974239 1102 28-29 0.3286675801407384 1102 30-31 0.2923291920460187 1102 32-33 0.2523938417878142 1102 34-35 0.28150639378872455 1102 36-37 0.19603786082799957 1102 38-39 0.3377556959490846 1102 40-41 0.3383035821379252 1102 42-43 0.37783268430207073 1102 44-45 0.3578640693509385 1102 46-47 0.34376463203823704 1102 48-49 0.14985810980800096 1102 50-51 0.2693830974282605 1102 52-53 0.3288648031680381 1102 54-55 0.3230967748791116 1102 56-57 0.32793271350842446 1102 58-59 0.3255753961207546 1102 60-61 0.35377867434725374 1102 62-63 0.3176764541383079 1102 64-65 0.2615925082222432 1102 66-67 0.24348165696746094 1102 68-69 0.2270378214478157 1102 70-71 0.18108319997856626 1102 72-73 0.16498300226181328 1102 74-75 0.13927974620843742 1102 76-77 0.16861858365030358 1102 78-79 0.23267765378466976 1102 80-81 0.24543397815187973 1102 82-83 0.27216171032017655 1102 84-85 0.24848323626097013 1102 86-87 0.16254582514542193 1102 88-89 0.10483461608989586 1102 90-91 0.06221460725168981 1102 92-93 0.08846832188451614 1102 94-95 0.06867563525075582 1102 96-97 0.008528963368416242 1102 98-99 -0.06910507541012834 1102 100-101 -0.1052047389009978 1103 1 0.3224572891095363 1103 2 0.3316466473704054 1103 3 0.28491064549738354 1103 4 0.23652625093024682 1103 5 0.2565555775387409 1103 6 0.28974450374259675 1103 7 0.26584551368496534 1103 8 0.24873373717296943 1103 9 0.31287105421817074 1103 10-11 0.2928244474319186 1103 12-13 0.2896882152747011 1103 14-15 0.37708902835102265 1103 16-17 0.3867968007881828 1103 18-19 0.3362442845321496 1103 20-21 0.3025365847627697 1103 22-23 0.2595909361905484 1103 24-25 0.3052016743025163 1103 26-27 0.2729125225640061 1103 28-29 0.2863071550285028 1103 30-31 0.27118529631720634 1103 32-33 0.2468466746994622 1103 34-35 0.2229545691605921 1103 36-37 0.2146912571138202 1103 38-39 0.2898636027638446 1103 40-41 0.33983518869325735 1103 42-43 0.32622245006206896 1103 44-45 0.2832226086063372 1103 46-47 0.36374556181702644 1103 48-49 0.33771298635031144 1103 50-51 0.3780836751087264 1103 52-53 0.3160534851618948 1103 54-55 0.32333284799402406 1103 56-57 0.3069684447412371 1103 58-59 0.2432631478310725 1103 60-61 0.24289304257384003 1103 62-63 0.1959980030267019 1103 64-65 0.21196254390055458 1103 66-67 0.1562817917633197 1103 68-69 0.17091736404437086 1103 70-71 0.1678468016784791 1103 72-73 0.14819239691352237 1103 74-75 0.17029262622311592 1103 76-77 0.2042331584937891 1103 78-79 0.27694021696974147 1103 80-81 0.18398361462288193 1103 82-83 0.24457232198754042 1103 84-85 0.25526633911613317 1103 86-87 0.23564148294774512 1103 88-89 0.16232661837171136 1103 90-91 0.19130247982264592 1103 92-93 0.17869521104899277 1103 94-95 0.16875264806408552 1103 96-97 0.19483700262331283 1103 98-99 0.15638884798676145 1103 100-101 0.16971109352645541 1104 1 0.34355408110582886 1104 2 0.3419820074590376 1104 3 0.3164703423513231 1104 4 0.24916895554260066 1104 5 0.23902258253353637 1104 6 0.2650382781954761 1104 7 0.30260129494988064 1104 8 0.3157860772137937 1104 9 0.3614448971923636 1104 10-11 0.29614414380459664 1104 12-13 0.28303691102075135 1104 14-15 0.395264992056525 1104 16-17 0.38793408761673476 1104 18-19 0.3486071034474705 1104 20-21 0.340503200892627 1104 22-23 0.32807551349780795 1104 24-25 0.3033356432735488 1104 26-27 0.3084618923358846 1104 28-29 0.28870470474335264 1104 30-31 0.2958495440650637 1104 32-33 0.29261141743779007 1104 34-35 0.25330303936522824 1104 36-37 0.312882713489806 1104 38-39 0.37977350221706274 1104 40-41 0.42061768773104546 1104 42-43 0.4623697927315149 1104 44-45 0.405706932745062 1104 46-47 0.4313150193086557 1104 48-49 0.3617736523650308 1104 50-51 0.3887590029229244 1104 52-53 0.3120138470988607 1104 54-55 0.327663942031144 1104 56-57 0.3505839785010565 1104 58-59 0.3510746039547996 1104 60-61 0.33820298179696096 1104 62-63 0.3451436325368462 1104 64-65 0.2907293123662811 1104 66-67 0.31675564509831133 1104 68-69 0.22809110137833244 1104 70-71 0.2645229625020633 1104 72-73 0.23357913952198572 1104 74-75 0.30840376275948955 1104 76-77 0.2291439144381897 1104 78-79 0.3999176080543876 1104 80-81 0.35306701790657513 1104 82-83 0.35464995999996773 1104 84-85 0.2827831246118464 1104 86-87 0.22685846099156848 1104 88-89 0.2713534022424007 1104 90-91 0.19288732341941994 1104 92-93 0.14789038788516606 1104 94-95 0.19902149125552526 1104 96-97 0.08042888352672861 1104 98-99 0.06890559139724672 1104 100-101 0.1121718719302045 1105 1 0.33984011381245693 1105 2 0.34689113389418225 1105 3 0.2837951226551496 1105 4 0.22117801113802926 1105 5 0.2118262342724293 1105 6 0.24410434086664878 1105 7 0.24695153431800776 1105 8 0.28162285345200644 1105 9 0.35528592868961795 1105 10-11 0.29606992644945507 1105 12-13 0.2837136482155884 1105 14-15 0.34392199422202197 1105 16-17 0.3294242513118135 1105 18-19 0.33077242063361467 1105 20-21 0.29591914622798043 1105 22-23 0.2306136759870867 1105 24-25 0.2576200526562289 1105 26-27 0.3244486328381626 1105 28-29 0.3137902075346304 1105 30-31 0.2924745441431682 1105 32-33 0.3649058809076351 1105 34-35 0.37871287493874917 1105 36-37 0.31946166915997765 1105 38-39 0.4321220913087558 1105 40-41 0.38725290696804393 1105 42-43 0.45997869037555716 1105 44-45 0.4089679603898233 1105 46-47 0.4668967648432698 1105 48-49 0.42164135202751396 1105 50-51 0.42923981671364686 1105 52-53 0.4595633916857125 1105 54-55 0.45933723600870024 1105 56-57 0.49464961060620993 1105 58-59 0.5132300640029825 1105 60-61 0.5259946176872603 1105 62-63 0.4879811438307087 1105 64-65 0.5610015538270616 1105 66-67 0.5709627173751954 1105 68-69 0.5678127566488129 1105 70-71 0.47479534828118375 1105 72-73 0.5664617659754398 1105 74-75 0.5550043126417279 1105 76-77 0.52875358848544 1105 78-79 0.539534771585906 1105 80-81 0.5059844570530316 1105 82-83 0.5779756293929168 1105 84-85 0.5360484187131789 1105 86-87 0.5552455653377528 1105 88-89 0.5420271285991412 1105 90-91 0.5935964317792468 1105 92-93 0.55459119392264 1105 94-95 0.5580826511463535 1105 96-97 0.5844392363209714 1105 98-99 0.5923350382881765 1105 100-101 0.7091612690536131 1106 1 0.27771738155017545 1106 2 0.24014404025648162 1106 3 0.22912776445060956 1106 4 0.22075163565237688 1106 5 0.22940829319725964 1106 6 0.2694704618295134 1106 7 0.2470199816477603 1106 8 0.2297655965418599 1106 9 0.30985879326816956 1106 10-11 0.2355447112658453 1106 12-13 0.2468486599189461 1106 14-15 0.30570618391642057 1106 16-17 0.30025087113788373 1106 18-19 0.3128886012439622 1106 20-21 0.27568681892218905 1106 22-23 0.3136469192816733 1106 24-25 0.33622398056466807 1106 26-27 0.34180191440594143 1106 28-29 0.3780121126908398 1106 30-31 0.34195838804145495 1106 32-33 0.40285578778739506 1106 34-35 0.3950814665760234 1106 36-37 0.4449172817335736 1106 38-39 0.4754957111199758 1106 40-41 0.48304868103336673 1106 42-43 0.40481857809031396 1106 44-45 0.36926838253768324 1106 46-47 0.459647146521732 1106 48-49 0.4090703490384797 1106 50-51 0.4571974138522279 1106 52-53 0.43804786220683667 1106 54-55 0.36652368196521223 1106 56-57 0.3381848072433371 1106 58-59 0.43777204552181104 1106 60-61 0.4850356250234711 1106 62-63 0.49930524322156344 1106 64-65 0.47989839987242533 1106 66-67 0.4961514903433084 1106 68-69 0.5460899646538238 1106 70-71 0.5553907328463694 1106 72-73 0.5285803465650574 1106 74-75 0.4698288482453634 1106 76-77 0.4143974168890949 1106 78-79 0.4296539987570114 1106 80-81 0.4684027077591253 1106 82-83 0.4510037083846399 1106 84-85 0.4370328474273393 1106 86-87 0.44887211915203906 1106 88-89 0.5271661350971435 1106 90-91 0.562058565029325 1106 92-93 0.5393108925972889 1106 94-95 0.6210288725906814 1106 96-97 0.5342178020458341 1106 98-99 0.6530200400702526 1106 100-101 0.6266854386060707 1107 1 0.19757701571598574 1107 2 0.19243070158275088 1107 3 0.18043481925670335 1107 4 0.1785421385577166 1107 5 0.18637553515907967 1107 6 0.16382185475627153 1107 7 0.17492514568960615 1107 8 0.1603096646855846 1107 9 0.26033530739528743 1107 10-11 0.17547241867178087 1107 12-13 0.17255055899462235 1107 14-15 0.2030939014696287 1107 16-17 0.19717553002201527 1107 18-19 0.19116995405214254 1107 20-21 0.18105123428325953 1107 22-23 0.18612362316402198 1107 24-25 0.22613432507838382 1107 26-27 0.2360678668614682 1107 28-29 0.23385597094677024 1107 30-31 0.25559901893515047 1107 32-33 0.2733356144352044 1107 34-35 0.27743242050099326 1107 36-37 0.3167513681740175 1107 38-39 0.33817468654976324 1107 40-41 0.32462588489372735 1107 42-43 0.32489833988579875 1107 44-45 0.28792228038766154 1107 46-47 0.28426442286705367 1107 48-49 0.19433965489690053 1107 50-51 0.2629530282156196 1107 52-53 0.2882261578732468 1107 54-55 0.324477834287741 1107 56-57 0.30064500733868016 1107 58-59 0.35914282742131576 1107 60-61 0.34395513535086053 1107 62-63 0.34403202944687905 1107 64-65 0.3800761965635857 1107 66-67 0.4076905917790441 1107 68-69 0.37470564774948656 1107 70-71 0.4413090222994498 1107 72-73 0.37410947052072885 1107 74-75 0.37339021535461825 1107 76-77 0.3585904104860127 1107 78-79 0.4961604807105928 1107 80-81 0.4989155983868301 1107 82-83 0.4827587460579963 1107 84-85 0.4844832237366319 1107 86-87 0.46343389209448915 1107 88-89 0.43093681870576006 1107 90-91 0.49013970823898134 1107 92-93 0.5372586459841493 1107 94-95 0.6059151173232387 1107 96-97 0.6204118343790448 1107 98-99 0.6901395919355267 1107 100-101 0.8202703091366992 1108 1 0.08132902969395417 1108 2 0.10876860251715925 1108 3 0.10003481929297209 1108 4 0.0846287748844432 1108 5 0.07708192795678315 1108 6 0.09600651373022373 1108 7 0.14450351427858976 1108 8 0.1278422326861275 1108 9 0.16513925126469786 1108 10-11 0.14677281606744685 1108 12-13 0.09421035533801358 1108 14-15 0.09724279333741492 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-0.9783408210196214 2316 50-51 -1.2062008654744432 2316 52-53 -1.1610894513416312 2316 54-55 -1.7739482763211285 2316 56-57 -1.243067831438907 2316 58-59 -0.8890864334160895 2316 60-61 -0.8228223959586103 2316 62-63 -1.3382572994147282 2316 64-65 -1.5728939156194457 2316 66-67 -1.0681096685273204 2316 68-69 -1.1215592443980213 2316 70-71 -0.9969363160514106 2316 72-73 -1.1097897828567724 2316 74-75 -0.8964692871710014 2316 76-77 -0.8414875470810479 2316 78-79 -0.8864596442743888 2316 80-81 -0.80525752193887 2316 82-83 -0.8835548330201348 2316 84-85 -1.0414892504856113 2316 86-87 -1.239674591806665 2316 88-89 -0.9449139777021038 2316 90-91 -0.8568191366456119 2316 92-93 -0.8371092576250518 2316 94-95 -1.136934024313767 2316 96-97 -1.1109201336761352 2316 98-99 -1.1204291901088652 2316 100-101 -1.4296372718316945 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 24.0 4 80.0 5 239.0 6 573.0 7 1543.0 8 4272.0 9 8746.0 10 14377.0 11 20971.0 12 29799.0 13 40736.0 14 50518.0 15 62365.0 16 76274.0 17 97799.0 18 84064.0 19 52872.0 20 43864.0 21 43824.0 22 45913.0 23 50816.0 24 60269.0 25 75031.0 26 95037.0 27 120597.0 28 154593.0 29 196212.0 30 248493.0 31 315539.0 32 404296.0 33 535531.0 34 747982.0 35 1116301.0 36 1749026.0 37 2687675.0 38 2737242.0 39 581417.0 40 176.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.82011199575037 21.89407666937953 15.757284580785727 23.52852675408437 2 14.848849503275153 21.925754836502072 38.808720590421 24.416675069801776 3 16.947938634090555 26.572249937057197 31.21353807539899 25.26627335345326 4 12.40339047607214 18.34293624135816 37.9986747963031 31.254998486266604 5 12.728865167762503 37.68961316063725 35.41161878527145 14.1699028863288 6 29.906871227859394 38.51374374605432 17.61182947753996 13.967555548546326 7 26.151919261431022 33.03288148472054 22.837223787324504 17.977975466523937 8 25.274838796417736 35.27805104018793 20.592426002304883 18.854684161089445 9 24.91008618902358 16.58587731706029 21.816692670751017 36.68734382316511 10-11 23.590409782171925 27.179142952835445 28.823870965429542 20.40657629956309 12-13 24.558697889011462 24.780110090015686 28.994022702904665 21.667169318068183 14-15 21.810733660708177 26.514407336599117 26.99352219632821 24.681336806364495 16-17 20.79018151204014 29.68999823975774 27.620741866446195 21.899078381755928 18-19 20.71705209482851 28.000829942608913 30.586778185890417 20.69533977667216 20-21 21.711234252705733 27.259750566586384 30.364219426597217 20.664795754110667 22-23 21.367015290212972 27.17379166537237 30.459196230373497 20.999996814041157 24-25 21.186828631815413 27.26865790699972 30.43413640878134 21.11037705240352 26-27 21.028161406487584 27.32986983525072 30.385528565933527 21.25644019232817 28-29 21.063106418335366 27.27233551505666 30.586126030267458 21.078432036340512 30-31 21.309820391274904 27.559247908276557 30.218989979894793 20.911941720553745 32-33 21.001092178329625 27.464853886150355 30.397223064012053 21.136830871507968 34-35 21.198659942268996 27.222356131700863 30.348426002498307 21.23055792353183 36-37 21.525442325663917 27.45629712320799 29.897246191561642 21.12101435956644 38-39 21.314893745310297 27.649349985764367 29.98633148286704 21.049424786058296 40-41 21.14132491415051 27.471407198876037 30.11115411362695 21.27611377334651 42-43 21.18181904710815 27.600145393333612 29.82731017115736 21.39072538840088 44-45 21.318932250119204 27.722744951874283 29.637339569158268 21.32098322884825 46-47 21.475815780553994 27.816082743983866 29.273798984955455 21.43430249050668 48-49 21.42069681934059 27.863068186253276 29.122361630411532 21.593873363994604 50-51 21.485781524039847 27.93813530151878 28.81815722440802 21.757925950033354 52-53 21.50255808076936 28.053431261174737 28.74180021262746 21.70221044542844 54-55 21.556887252974168 28.37018754498959 28.657455851708335 21.415469350327907 56-57 21.551292830976124 28.05899388720734 28.67464190842205 21.715071373394487 58-59 21.38020648521373 27.788510561258068 28.92079731478899 21.910485638739207 60-61 21.631110315282104 28.045435906487825 28.669968737514285 21.653485040715786 62-63 21.436040999953324 28.062885541451816 28.71523247065293 21.78584098794193 64-65 21.422503818014786 27.989580845935397 28.81693552999079 21.770979806059028 66-67 21.378476431392798 28.056886360379636 28.677619291926604 21.88701791630096 68-69 21.648693695575627 28.163131859993513 28.50530088574913 21.68287355868173 70-71 21.653899415824 27.941246750838605 28.558023885408407 21.84682994792899 72-73 21.443896265998237 28.113533591416118 28.602139121214147 21.840431021371497 74-75 21.519147955945588 28.196467509743 28.416788162096996 21.86759637221442 76-77 21.517580449336336 28.268147382676002 28.3254958431526 21.88877632483506 78-79 21.54023230480275 28.234472586901433 28.443381474055784 21.78191363424003 80-81 21.477202137703067 28.19636939025984 28.39296084395575 21.93346762808134 82-83 21.351066885685746 28.161395031593013 28.559214382746795 21.928323699974445 84-85 21.329727812544565 28.193862599263696 28.360911510552505 22.115498077639234 86-87 21.42596877395931 28.30037015842022 28.30959395043502 21.96406711718545 88-89 21.227960662272743 28.140625787798374 28.419770753212703 22.21164279671618 90-91 21.167448450850266 28.12233483796074 28.423419618523727 22.28679709266527 92-93 21.240408212566447 28.212818934237475 28.29030153659358 22.256471316602493 94-95 21.093252098470604 27.99847694588542 28.47344569647408 22.434825259169898 96-97 21.144436290579225 28.111940892012854 28.364046528033786 22.379576289374132 98-99 20.99003092149102 28.118497124099022 28.313319562482647 22.57815239192731 100-101 20.937846908673478 28.107936324617413 28.266676755538345 22.68754001117077 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 6994.0 1 4830.0 2 3625.0 3 4636.5 4 3724.0 5 3117.0 6 5353.5 7 10737.5 8 16408.0 9 18640.0 10 16126.5 11 12935.0 12 12624.5 13 13348.0 14 14214.5 15 15105.0 16 16099.5 17 17174.0 18 18431.0 19 20296.5 20 23111.5 21 27271.0 22 33345.5 23 42912.0 24 55502.0 25 70214.0 26 90404.5 27 116362.0 28 149145.0 29 184547.0 30 214543.5 31 245479.5 32 277553.5 33 308172.0 34 336990.5 35 359827.5 36 377501.5 37 395642.0 38 414144.5 39 424430.5 40 433826.0 41 441344.0 42 440342.0 43 440435.0 44 438519.5 45 433506.5 46 428173.0 47 418053.0 48 407699.5 49 402503.0 50 394079.5 51 375586.0 52 347684.0 53 323545.0 54 308530.0 55 292565.0 56 269064.5 57 240195.5 58 215500.0 59 195876.0 60 174144.5 61 149346.0 62 126658.5 63 104533.0 64 84350.0 65 68148.5 66 54188.5 67 41954.5 68 32265.0 69 24806.5 70 18402.5 71 13636.0 72 10434.0 73 7790.5 74 5875.5 75 4337.0 76 2950.5 77 2035.5 78 1416.0 79 933.0 80 603.5 81 389.5 82 244.0 83 179.5 84 134.5 85 89.0 86 58.5 87 43.5 88 30.0 89 18.5 90 12.0 91 9.5 92 9.0 93 6.5 94 3.0 95 1.5 96 1.5 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.049549624893170816 2 0.002007154070580139 3 3.2656078132454645E-4 4 3.10630987113593E-4 5 0.0025328372795416043 6 0.0017204177747829764 7 0.0 8 2.389469131643023E-5 9 8.761386816024416E-5 10-11 3.9824485527383716E-5 12-13 3.9824485527383715E-6 14-15 5.017885176450348E-4 16-17 0.0 18-19 3.9824485527383716E-5 20-21 6.053321800162325E-4 22-23 0.0 24-25 6.730338054127848E-4 26-27 0.007351600028355034 28-29 0.007542757558886476 30-31 0.00105136641792293 32-33 0.006543162972149144 34-35 0.00799675669389865 36-37 0.004520079107358052 38-39 0.002600538904938157 40-41 0.002142557321373244 42-43 0.0014456288246440289 44-45 8.641913359442267E-4 46-47 5.933848343580173E-4 48-49 0.002166452012689674 50-51 0.0013978394420111684 52-53 0.0021306099757150286 54-55 8.164019533113662E-4 56-57 2.110697732951337E-4 58-59 7.885248134421976E-4 60-61 0.00594181324068565 62-63 0.0027399246042839995 64-65 0.001083226006344837 66-67 0.00523293739829822 68-69 0.007283898402958481 70-71 0.002485047896908744 72-73 5.933848343580173E-4 74-75 0.0034567653437769068 76-77 0.002094767938740383 78-79 0.0026642580817819706 80-81 9.478227555517325E-4 82-83 1.553154935567965E-4 84-85 6.730338054127848E-4 86-87 0.0047829207118387845 88-89 0.0025726617650689877 90-91 0.0012305766027961567 92-93 1.0752611092393603E-4 94-95 4.261219951430057E-4 96-97 2.8275384724442437E-4 98-99 5.416130031724185E-4 100-101 1.9912242763691858E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.255509E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.70298178641189 #Duplication Level Percentage of deduplicated Percentage of total 1 65.77474453280301 29.40327206855788 2 18.17698174493426 16.25130567751475 3 6.813077644718541 9.136946575837888 4 3.022951577927929 5.405397973172691 5 1.646897070701548 3.681060487783319 6 1.004281643155394 2.693663040144203 7 0.6555460130949754 2.051340303847773 8 0.47858938251315053 1.7115497959724384 9 0.3411505250878162 1.3725401138482947 >10 1.866786037242601 15.245286389019306 >50 0.13977767108699254 4.3160809207915385 >100 0.07467713516383287 5.947261786059109 >500 0.0033767427141318408 1.0139284469842047 >1k 0.0010549940612773962 0.761682173308173 >5k 5.3642397146110356E-5 0.18697011874988137 >10k+ 5.3642397146110356E-5 0.8217141284086423 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 42592 0.3392408975164654 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 35704 0.28437868625394164 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24505 0.19517980356970757 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.5929794210953486E-5 0.0 5 0.0 7.964897105476743E-6 0.0 1.5929794210953486E-5 0.0 6 0.0 7.964897105476743E-6 0.0 1.5929794210953486E-5 0.0 7 0.0 7.964897105476743E-6 0.0 1.5929794210953486E-5 0.0 8 0.0 7.964897105476743E-6 0.0 1.5929794210953486E-5 0.0 9 0.0 7.964897105476743E-6 0.0 1.5929794210953486E-5 0.0 10-11 0.0 7.964897105476743E-6 0.0 1.5929794210953486E-5 0.0 12-13 0.0 7.964897105476743E-6 0.0 2.78771398691686E-5 0.0 14-15 0.0 7.964897105476743E-6 0.0 3.9824485527383716E-5 0.0 16-17 0.0 7.964897105476743E-6 0.0 3.9824485527383716E-5 0.0 18-19 0.0 7.964897105476743E-6 0.0 5.57542797383372E-5 0.0 20-21 0.0 7.964897105476743E-6 0.0 6.770162539655232E-5 0.0 22-23 0.0 1.5929794210953486E-5 0.0 1.4735059645131973E-4 1.5929794210953486E-5 24-25 0.0 1.5929794210953486E-5 0.0 3.424905755355E-4 1.5929794210953486E-5 26-27 0.0 1.5929794210953486E-5 0.0 6.21261974227186E-4 1.5929794210953486E-5 28-29 0.0 1.5929794210953486E-5 0.0 0.0014735059645131976 1.5929794210953486E-5 30-31 0.0 1.5929794210953486E-5 0.0 0.003982448552738371 1.5929794210953486E-5 32-33 0.0 2.3894691316430227E-5 0.0 0.009458315312753632 1.5929794210953486E-5 34-35 0.0 2.3894691316430227E-5 0.0 0.018952472662481908 1.5929794210953486E-5 36-37 0.0 2.3894691316430227E-5 0.0 0.03491014401330456 1.5929794210953486E-5 38-39 0.0 2.3894691316430227E-5 0.0 0.055774191981100896 1.5929794210953486E-5 40-41 0.0 2.3894691316430227E-5 0.0 0.07760995739576539 1.5929794210953486E-5 42-43 0.0 2.3894691316430227E-5 0.0 0.10021433538110838 1.5929794210953486E-5 44-45 0.0 2.3894691316430227E-5 0.0 0.12831847481778305 1.5929794210953486E-5 46-47 0.0 2.3894691316430227E-5 0.0 0.16309321558029452 1.5929794210953486E-5 48-49 0.0 2.3894691316430227E-5 0.0 0.1996759879857492 1.5929794210953486E-5 50-51 0.0 3.185958842190697E-5 0.0 0.2373738459859706 1.5929794210953486E-5 52-53 0.0 3.185958842190697E-5 0.0 0.27527879131093447 1.5929794210953486E-5 54-55 0.0 3.5842036974645344E-5 0.0 0.3190299711113182 1.5929794210953486E-5 56-57 0.0 5.1771831185598826E-5 0.0 0.3724505359977507 1.5929794210953486E-5 58-59 0.0 5.57542797383372E-5 0.0 0.43256957934988916 1.5929794210953486E-5 60-61 0.0 5.57542797383372E-5 0.0 0.49636044026765236 1.5929794210953486E-5 62-63 0.0 5.57542797383372E-5 0.0 0.562429261757582 1.5929794210953486E-5 64-65 0.0 7.168407394929069E-5 0.0 0.6346230891216231 1.5929794210953486E-5 66-67 0.0 7.168407394929069E-5 0.0 0.7216356075504039 1.5929794210953486E-5 68-69 0.0 7.168407394929069E-5 0.0 0.8201255427081766 1.5929794210953486E-5 70-71 0.0 7.168407394929069E-5 0.0 0.9290136510371491 1.5929794210953486E-5 72-73 0.0 7.168407394929069E-5 0.0 1.0444289925440597 1.5929794210953486E-5 74-75 0.0 7.168407394929069E-5 0.0 1.1666304263848368 1.5929794210953486E-5 76-77 0.0 7.168407394929069E-5 0.0 1.3030332717646786 1.5929794210953486E-5 78-79 0.0 7.168407394929069E-5 0.0 1.4593443774596597 1.5929794210953486E-5 80-81 0.0 7.168407394929069E-5 0.0 1.634149974233558 1.5929794210953486E-5 82-83 0.0 7.168407394929069E-5 0.0 1.8227228956542727 1.5929794210953486E-5 84-85 0.0 7.168407394929069E-5 0.0 2.0168433679089515 1.5929794210953486E-5 86-87 0.0 7.168407394929069E-5 0.0 2.227264002089989 1.5929794210953486E-5 88-89 0.0 7.168407394929069E-5 0.0 2.466151178526 1.5929794210953486E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 21345 0.0 55.16791 1 GTATCAA 39885 0.0 40.890186 1 ATCAACG 50905 0.0 31.71448 3 TCAACGC 51490 0.0 31.298685 4 TATCAAC 53190 0.0 30.441832 2 CAACGCA 53760 0.0 30.030117 5 AACGCAG 56615 0.0 28.607805 6 GTGGTAT 9795 0.0 26.101124 1 ACGCAGA 65100 0.0 24.813065 7 CGCAGAG 65750 0.0 24.430513 8 GTACATG 37405 0.0 24.074701 1 TGGTATC 10435 0.0 23.487186 2 TACATGG 37335 0.0 22.670721 2 GCAGAGT 71370 0.0 21.954477 9 ACATGGG 38560 0.0 20.755335 3 AGAGTAC 65495 0.0 20.167294 10-11 GAGTACT 48665 0.0 19.739143 12-13 CAGAGTA 68740 0.0 19.280897 10-11 AGTACTT 50365 0.0 18.756958 12-13 GTACTTT 52010 0.0 18.218641 14-15 >>END_MODULE