FastQCFastQC Report
Wed 25 May 2016
SRR2049411_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049411_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8006890
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT307840.3844688761803896No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258290.3225846739495609No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132910.16599453720483234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA150750.057.2912141
GTATCAA284500.042.329891
ATCAACG343500.034.4896353
TCAACGC344700.034.4253354
CAACGCA358100.033.1226465
TATCAAC359900.033.0083352
GTGGTAT59650.032.5734941
AACGCAG373200.031.9731946
ACGCAGA419300.028.1990977
TGGTATC65850.028.0598682
CGCAGAG420500.027.81368
TACCTGG60100.025.6862472
GCAGAGT446600.025.3052679
GTACCTG66400.024.8978961
GTACATG240650.022.4058531
GAGTACT301650.022.32326112-13
TATAACG8150.022.1471462
CAGAGTA430900.022.13125810-11
AGAGTAC408900.021.85237710-11
TACATGG238850.021.3982772
Produced by FastQC (version 0.11.5)