FastQCFastQC Report
Wed 25 May 2016
SRR2049411_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049411_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8006890
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT295210.36869496146443875No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257610.32173540538211465No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158560.19802944713865184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA147100.057.5041281
GTATCAA273400.041.125191
ATCAACG338800.032.832963
TCAACGC340400.032.6228184
TATCAAC355700.031.3533152
CAACGCA354450.031.3296895
TATAACG8250.030.5134412
AACGCAG372000.030.1066196
GTGGTAT59600.028.624631
ACGCAGA428650.025.9390627
CGCAGAG431650.025.6487528
ATAACGC10250.025.4862163
TGGTATC65200.025.278492
GCAGAGT466700.023.264819
TACCTGG58650.021.2179072
AGAGTAC433500.021.04744510-11
GTACATG232750.020.8870771
GTACCTG65100.020.8774151
GAGTACT321000.020.87032112-13
CAGAGTA453650.020.66747310-11
Produced by FastQC (version 0.11.5)