##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049411_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8006890 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06026047566533 33.0 31.0 34.0 30.0 34.0 2 32.25230782488582 34.0 31.0 34.0 30.0 34.0 3 32.32367735787553 34.0 31.0 34.0 30.0 34.0 4 35.960584696430196 37.0 35.0 37.0 35.0 37.0 5 35.846083685425924 37.0 35.0 37.0 35.0 37.0 6 35.87023151310933 37.0 35.0 37.0 35.0 37.0 7 35.796021801223695 37.0 35.0 37.0 35.0 37.0 8 35.78995527601853 37.0 35.0 37.0 35.0 37.0 9 37.39918170475678 39.0 37.0 39.0 34.0 39.0 10-11 37.394567990817905 39.0 37.0 39.0 34.0 39.0 12-13 37.37304871179697 39.0 37.0 39.0 34.0 39.0 14-15 38.60294171644671 40.0 38.0 41.0 34.0 41.0 16-17 38.58953769815746 40.0 38.0 41.0 34.0 41.0 18-19 38.62355939696936 40.0 38.0 41.0 34.0 41.0 20-21 38.61185922374355 40.0 38.0 41.0 34.0 41.0 22-23 38.52697901432391 40.0 38.0 41.0 34.0 41.0 24-25 38.4279368768648 40.0 38.0 41.0 34.0 41.0 26-27 38.24842835358048 40.0 38.0 41.0 34.0 41.0 28-29 38.06575992676308 40.0 38.0 41.0 33.0 41.0 30-31 37.881363737980664 40.0 37.0 41.0 33.0 41.0 32-33 37.63591150871312 40.0 37.0 41.0 33.0 41.0 34-35 37.45241161050046 40.0 36.5 41.0 32.5 41.0 36-37 37.26528208830145 39.0 36.0 41.0 32.0 41.0 38-39 37.41576417310591 40.0 37.0 41.0 33.0 41.0 40-41 37.33005879935905 40.0 37.0 41.0 32.0 41.0 42-43 37.14963100529668 40.0 36.5 41.0 31.5 41.0 44-45 36.9191711763244 40.0 36.0 41.0 31.0 41.0 46-47 36.60866503723668 39.5 36.0 41.0 30.5 41.0 48-49 36.348470692116415 39.0 36.0 41.0 30.0 41.0 50-51 36.128574702537435 39.0 35.0 41.0 29.5 41.0 52-53 35.95148896762663 39.0 35.0 40.0 29.0 41.0 54-55 35.844657476248585 39.0 35.0 40.0 29.0 41.0 56-57 35.75893724030179 39.0 35.0 40.0 28.5 41.0 58-59 35.531765579394744 38.5 35.0 40.0 28.0 41.0 60-61 35.287182788823124 38.0 35.0 40.0 28.0 41.0 62-63 35.01003616884958 38.0 34.0 40.0 27.5 41.0 64-65 34.754916890328204 37.5 34.0 40.0 27.0 41.0 66-67 34.42600273014866 37.0 34.0 40.0 26.0 41.0 68-69 34.10947702541186 36.5 34.0 39.5 26.0 41.0 70-71 33.780529206720715 36.0 33.5 39.0 26.0 41.0 72-73 33.41335206303571 35.5 33.0 39.0 25.5 40.0 74-75 33.04186781634317 35.0 33.0 38.0 25.0 40.0 76-77 31.02367941860073 33.5 30.0 35.5 22.0 38.5 78-79 32.34878685731914 35.0 32.5 37.0 25.0 39.0 80-81 32.36166139412431 35.0 33.0 36.5 25.0 39.0 82-83 32.08697634162577 35.0 33.0 36.0 24.5 38.5 84-85 31.818402900501944 35.0 33.0 36.0 24.0 37.0 86-87 31.54976370351035 35.0 32.0 35.5 23.5 37.0 88-89 31.283471485183384 35.0 32.0 35.0 23.0 36.5 90-91 31.01977677974844 35.0 32.0 35.0 20.5 36.0 92-93 30.77544158843196 34.5 32.0 35.0 20.0 36.0 94-95 30.52184924483788 34.0 32.0 35.0 18.0 36.0 96-97 30.281644870854976 34.0 31.0 35.0 12.5 35.0 98-99 29.997941835094522 34.0 31.0 35.0 2.0 35.0 100-101 29.38778857958583 34.0 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3512622137859722 1101 2 0.368285714766742 1101 3 0.3398669520397348 1101 4 0.29154761280674535 1101 5 0.28661253597257996 1101 6 0.33670160500098234 1101 7 0.322883215358047 1101 8 0.36514910770951303 1101 9 0.4257485935679739 1101 10-11 0.3903117748939948 1101 12-13 0.37237516164969264 1101 14-15 0.4538812362299538 1101 16-17 0.40188452043462064 1101 18-19 0.4039997896274272 1101 20-21 0.34430034267965226 1101 22-23 0.2971974318094297 1101 24-25 0.272563466359955 1101 26-27 0.298063916043354 1101 28-29 0.25982611233033026 1101 30-31 0.16267162724494 1101 32-33 0.2128152511615582 1101 34-35 0.1896136960015653 1101 36-37 0.1513185939363879 1101 38-39 0.26032954488619 1101 40-41 0.2661454782833701 1101 42-43 0.33353081806075835 1101 44-45 0.24712220441473676 1101 46-47 0.27165541639772783 1101 48-49 0.18724147502460653 1101 50-51 0.19883046775417057 1101 52-53 0.2092875443169362 1101 54-55 0.19917214844181785 1101 56-57 0.20496969325703418 1101 58-59 0.1378736739393247 1101 60-61 0.1593715468471757 1101 62-63 0.26511180072606777 1101 64-65 0.10626488701323211 1101 66-67 0.0849812236847356 1101 68-69 0.05078208306402843 1101 70-71 0.022547739315612603 1101 72-73 0.0020506332492047363 1101 74-75 -0.051511627652644165 1101 76-77 -0.05569592789620259 1101 78-79 0.041709444854902245 1101 80-81 0.004987798769079177 1101 82-83 0.028929331874593345 1101 84-85 0.017630095856031147 1101 86-87 -0.014968675664675857 1101 88-89 -0.10090247869973368 1101 90-91 -0.13324878057698086 1101 92-93 -0.21131700451906354 1101 94-95 -0.2113216041963426 1101 96-97 -0.3192310750832732 1101 98-99 -0.3738339971274627 1101 100-101 -0.3520831898401511 1102 1 0.3398233102161896 1102 2 0.3519261033888057 1102 3 0.32216103016133246 1102 4 0.26159807722922324 1102 5 0.26581716704108516 1102 6 0.26413078191251316 1102 7 0.2967482936584176 1102 8 0.31337039928748567 1102 9 0.41878720252476853 1102 10-11 0.3439830780626565 1102 12-13 0.2955290518936593 1102 14-15 0.3465165397471637 1102 16-17 0.36151248864410945 1102 18-19 0.31573965281034333 1102 20-21 0.3535502187180555 1102 22-23 0.3113982916542639 1102 24-25 0.31735219153934224 1102 26-27 0.2632586608165326 1102 28-29 0.24475683323682773 1102 30-31 0.1949665893609236 1102 32-33 0.233242070528064 1102 34-35 0.18038096556858818 1102 36-37 0.13187644879480587 1102 38-39 0.2848659424484481 1102 40-41 0.32017471337015024 1102 42-43 0.3744237184492576 1102 44-45 0.341508309007331 1102 46-47 0.36949206194146456 1102 48-49 0.16536651580410933 1102 50-51 0.14570960166245328 1102 52-53 0.16461250793959437 1102 54-55 0.15917458379778537 1102 56-57 0.1565426916517012 1102 58-59 0.23919678687278179 1102 60-61 0.26179223757448966 1102 62-63 0.21152559092194423 1102 64-65 0.1909598469402809 1102 66-67 0.13293029356235309 1102 68-69 0.08154325825069719 1102 70-71 -0.038904822453169174 1102 72-73 -0.044889039703299716 1102 74-75 0.004124542470492543 1102 76-77 0.00816252336052159 1102 78-79 0.08175272301824066 1102 80-81 0.09674759003501521 1102 82-83 0.18859185681706947 1102 84-85 0.14556966457492493 1102 86-87 0.025522549654841242 1102 88-89 0.0402508875589902 1102 90-91 -0.06534585261595183 1102 92-93 -0.048437711427030905 1102 94-95 -0.12180673772596151 1102 96-97 -0.11216189015297573 1102 98-99 -0.14883838552817608 1102 100-101 -0.20645768625882255 1103 1 0.3296722902674887 1103 2 0.3769175284165982 1103 3 0.28115859391467524 1103 4 0.22931242510147598 1103 5 0.2505226275095751 1103 6 0.2673317415308176 1103 7 0.24078442870888495 1103 8 0.22769151304989776 1103 9 0.3403834991395982 1103 10-11 0.30526205858406996 1103 12-13 0.2953234503217743 1103 14-15 0.4110888094510159 1103 16-17 0.3581502835774941 1103 18-19 0.32324912817994544 1103 20-21 0.3045380179889463 1103 22-23 0.25327488222070116 1103 24-25 0.2381278959752322 1103 26-27 0.24256770094643798 1103 28-29 0.24336401047026612 1103 30-31 0.2822962287953885 1103 32-33 0.3488357947212961 1103 34-35 0.298968186984375 1103 36-37 0.2517277691240807 1103 38-39 0.3868699454153699 1103 40-41 0.3848990956922478 1103 42-43 0.397019998449025 1103 44-45 0.47526742686906687 1103 46-47 0.5081362395162543 1103 48-49 0.4684129554119636 1103 50-51 0.4583894096073564 1103 52-53 0.4665911928017863 1103 54-55 0.4570064032224366 1103 56-57 0.41447417306509493 1103 58-59 0.3610247537224609 1103 60-61 0.3648525967676832 1103 62-63 0.3926859499173787 1103 64-65 0.4203146018287427 1103 66-67 0.3862865385425067 1103 68-69 0.37141279923777404 1103 70-71 0.3460640917445872 1103 72-73 0.3813846440102395 1103 74-75 0.32700073865127877 1103 76-77 0.3625951778637564 1103 78-79 0.4684486063324158 1103 80-81 0.39561043045230093 1103 82-83 0.42087964383144083 1103 84-85 0.42540501265105846 1103 86-87 0.4191417488225859 1103 88-89 0.43636535809301336 1103 90-91 0.4091563213324001 1103 92-93 0.3513767075972076 1103 94-95 0.3378262779110166 1103 96-97 0.3305233568477206 1103 98-99 0.27189997785652764 1103 100-101 0.29394861178439413 1104 1 0.2867895736128858 1104 2 0.30605381784207 1104 3 0.3024427994077499 1104 4 0.2414105943208824 1104 5 0.24390943442129753 1104 6 0.28316949121015256 1104 7 0.26764618409148255 1104 8 0.2511656145920753 1104 9 0.3307967418119233 1104 10-11 0.2934006437411938 1104 12-13 0.2296830688709406 1104 14-15 0.29452651196087487 1104 16-17 0.36450862825807917 1104 18-19 0.3088833134540394 1104 20-21 0.2516135942114275 1104 22-23 0.2577284147365546 1104 24-25 0.2823145032703138 1104 26-27 0.274735778673147 1104 28-29 0.2895951899340261 1104 30-31 0.29222538555683286 1104 32-33 0.2928889512959074 1104 34-35 0.28887366114372526 1104 36-37 0.2896679113861538 1104 38-39 0.32421107768770696 1104 40-41 0.29674954893629746 1104 42-43 0.2787936826974189 1104 44-45 0.3143241674947461 1104 46-47 0.4769988203647202 1104 48-49 0.4113203802511549 1104 50-51 0.4877411216594041 1104 52-53 0.40383055948691293 1104 54-55 0.4201238395515716 1104 56-57 0.3594536617201527 1104 58-59 0.43734910418485384 1104 60-61 0.42183190512265867 1104 62-63 0.39186344129627315 1104 64-65 0.3452635635514625 1104 66-67 0.3832389213937901 1104 68-69 0.42315489164312226 1104 70-71 0.4185699461621155 1104 72-73 0.3718891418784622 1104 74-75 0.3320748186940321 1104 76-77 0.29205132159066594 1104 78-79 0.4337058104701583 1104 80-81 0.41801065048303343 1104 82-83 0.3885011220043353 1104 84-85 0.39333710756610074 1104 86-87 0.3513175306127785 1104 88-89 0.35981558679425163 1104 90-91 0.40562911327524986 1104 92-93 0.27140061732741216 1104 94-95 0.2754496740207273 1104 96-97 0.25016725381485116 1104 98-99 0.3090874724450856 1104 100-101 0.33597468200594705 1105 1 0.32658345536918176 1105 2 0.27248682086614195 1105 3 0.23148829780246416 1105 4 0.20100411192948542 1105 5 0.21973161164576993 1105 6 0.21640984094767646 1105 7 0.24477276627261801 1105 8 0.2628109447799716 1105 9 0.2912686798241424 1105 10-11 0.24073725943871693 1105 12-13 0.2501816303168951 1105 14-15 0.26485314297702445 1105 16-17 0.22852663082192493 1105 18-19 0.279643627086827 1105 20-21 0.26195850741512317 1105 22-23 0.1569680548402843 1105 24-25 0.21791593547964538 1105 26-27 0.212562552084691 1105 28-29 0.2535054962127177 1105 30-31 0.31222403850836145 1105 32-33 0.3140806088360506 1105 34-35 0.29791340333955674 1105 36-37 0.26854244968257746 1105 38-39 0.29897108736338396 1105 40-41 0.3006744348217012 1105 42-43 0.34694701292328745 1105 44-45 0.3005575138419374 1105 46-47 0.2996171251072113 1105 48-49 0.2860627842676422 1105 50-51 0.31022140950960875 1105 52-53 0.276881379744502 1105 54-55 0.2614515706172469 1105 56-57 0.2822983886682593 1105 58-59 0.2594878984238562 1105 60-61 0.2654664041275723 1105 62-63 0.2792673424255554 1105 64-65 0.23971178618048583 1105 66-67 0.2912375684572339 1105 68-69 0.1578284075285623 1105 70-71 0.07714779553013074 1105 72-73 0.15589030213060795 1105 74-75 0.19658432571995377 1105 76-77 0.22426101702312806 1105 78-79 0.2989468288051569 1105 80-81 0.31206111979904705 1105 82-83 0.3350697338162121 1105 84-85 0.2586635406105735 1105 86-87 0.2701089379335606 1105 88-89 0.272875121109621 1105 90-91 0.3107109677993023 1105 92-93 0.27124060274900685 1105 94-95 0.2953996601897515 1105 96-97 0.3044268785422872 1105 98-99 0.2713750344291448 1105 100-101 0.2938260714966603 1106 1 0.31409460136105594 1106 2 0.28393028641252016 1106 3 0.2608737413754838 1106 4 0.22300090757507718 1106 5 0.21635075285760053 1106 6 0.2821411549978734 1106 7 0.28123464134214515 1106 8 0.25587232928207015 1106 9 0.3468153385997539 1106 10-11 0.33324035670381846 1106 12-13 0.3058976945462675 1106 14-15 0.39628371239700755 1106 16-17 0.42695813944923344 1106 18-19 0.3528233547394066 1106 20-21 0.29981974842511505 1106 22-23 0.3387918955700968 1106 24-25 0.32306531182630494 1106 26-27 0.31295003376030905 1106 28-29 0.3903577439908972 1106 30-31 0.3488916234438051 1106 32-33 0.44570093446324677 1106 34-35 0.44511134904110605 1106 36-37 0.4760855029918716 1106 38-39 0.5386745824104082 1106 40-41 0.534432300966877 1106 42-43 0.5340133863493932 1106 44-45 0.49854769341141747 1106 46-47 0.571063988752627 1106 48-49 0.5983557821402314 1106 50-51 0.6252279673109697 1106 52-53 0.6563687208795912 1106 54-55 0.6337620811873066 1106 56-57 0.5558285897464188 1106 58-59 0.6189239048022941 1106 60-61 0.6922563357644762 1106 62-63 0.6808915202407348 1106 64-65 0.6645859997551682 1106 66-67 0.7149112540778333 1106 68-69 0.7141030919935005 1106 70-71 0.6925152859394004 1106 72-73 0.6738865967647172 1106 74-75 0.7049910259538876 1106 76-77 0.5867482968562037 1106 78-79 0.5862863291710738 1106 80-81 0.6632884844105362 1106 82-83 0.6735788689508126 1106 84-85 0.6749801147818495 1106 86-87 0.6679369396318542 1106 88-89 0.7081787968840523 1106 90-91 0.6781882236186405 1106 92-93 0.6130951853012441 1106 94-95 0.5942633631186993 1106 96-97 0.6144011316983935 1106 98-99 0.6047715138774556 1106 100-101 0.692244895905759 1107 1 0.16805438141211937 1107 2 0.18955912147951892 1107 3 0.17415269836237712 1107 4 0.16468108368731293 1107 5 0.1821189291523666 1107 6 0.18466550401629434 1107 7 0.18195613626478746 1107 8 0.13892039287913605 1107 9 0.2196049379090823 1107 10-11 0.13237489841676364 1107 12-13 0.18885291796548387 1107 14-15 0.1881605869199845 1107 16-17 0.1861145921507159 1107 18-19 0.21121930404016354 1107 20-21 0.14789780070972114 1107 22-23 0.20969020929982918 1107 24-25 0.1928405346714399 1107 26-27 0.16320522280790328 1107 28-29 0.22010826637330183 1107 30-31 0.22521341100362946 1107 32-33 0.26905639222773914 1107 34-35 0.24488286240077883 1107 36-37 0.28570427239950646 1107 38-39 0.26402773793867595 1107 40-41 0.32216497365850927 1107 42-43 0.3692500758102639 1107 44-45 0.2593876048253705 1107 46-47 0.2505866059418267 1107 48-49 0.14011097965193642 1107 50-51 0.129940934833094 1107 52-53 0.15951111875282464 1107 54-55 0.19491441371028628 1107 56-57 0.2859144451986637 1107 58-59 0.29445469610767105 1107 60-61 0.361264894813381 1107 62-63 0.33631381645640346 1107 64-65 0.35653436283431716 1107 66-67 0.39088073619980435 1107 68-69 0.40397169230306673 1107 70-71 0.3724390501250454 1107 72-73 0.354783460556682 1107 74-75 0.37665188182933207 1107 76-77 0.4186676022992124 1107 78-79 0.4520608204232417 1107 80-81 0.378481035410438 1107 82-83 0.37180080363884827 1107 84-85 0.42541939440565457 1107 86-87 0.399815286172867 1107 88-89 0.3616477177991442 1107 90-91 0.46485877641293527 1107 92-93 0.5014554013640513 1107 94-95 0.6080945476736979 1107 96-97 0.5966556230094398 1107 98-99 0.6348295408286333 1107 100-101 0.7982085929968292 1108 1 0.16146150178650487 1108 2 0.1542329896106338 1108 3 0.1458508467866082 1108 4 0.1206506971048853 1108 5 0.13085478586944532 1108 6 0.1661820663007063 1108 7 0.16842915042324336 1108 8 0.13968919002841318 1108 9 0.19606667281419732 1108 10-11 0.12369818301626623 1108 12-13 0.13313772367094856 1108 14-15 0.18565202541314108 1108 16-17 0.19853046489208026 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2692.0 9 5419.0 10 8892.0 11 13280.0 12 19097.0 13 26925.0 14 32458.0 15 39540.0 16 49810.0 17 65567.0 18 55534.0 19 33714.0 20 28027.0 21 27258.0 22 28854.0 23 31637.0 24 37560.0 25 46448.0 26 58694.0 27 75435.0 28 95948.0 29 122730.0 30 155098.0 31 196104.0 32 252033.0 33 334742.0 34 465760.0 35 694312.0 36 1083871.0 37 1694830.0 38 1813467.0 39 409479.0 40 135.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.50610791546158 22.23154523850997 16.251745930909493 23.010600915118957 2 14.469077888226527 21.88774943079135 39.36458454325299 24.278588137729134 3 16.41218942928025 26.895709085895213 31.83969969795945 24.852401786865084 4 12.160964711192497 18.88393883604748 38.624391819378175 30.330704633381846 5 12.418215185766089 37.704744612062775 35.563270778082604 14.31376942408853 6 28.943300472313926 39.21750532769159 17.985759766976642 13.853434433017837 7 25.573624715713596 33.48835815154199 23.067595533346903 17.870421599397517 8 24.977278940672214 35.547251872731096 20.77737058675348 18.69809859984321 9 24.5690142899642 16.902018489603563 22.272463749291546 36.25650347114069 10-11 23.268776196590274 27.505216915463958 28.99271146156499 20.233295426380778 12-13 24.350284567416313 25.065512327507932 29.253780181818406 21.330422923257345 14-15 21.59037723047506 26.85088410319049 27.27558335261372 24.283155313720734 16-17 20.4359807615691 30.154510677678847 27.858356990042328 21.551151570709727 18-19 20.353402103314835 28.28636229944604 31.01524500476777 20.344990592471355 20-21 21.412138649814455 27.526449967137566 30.80929015578824 20.252121227259742 22-23 21.067280804407204 27.473213694705436 30.883589008966027 20.575916491921333 24-25 20.842267348016424 27.538032425227794 30.837481447140526 20.78221877961526 26-27 20.704801246470662 27.547537494135078 30.832550250674924 20.915111008719336 28-29 20.789473207610705 27.498191887101562 30.954299536657214 20.75803536863052 30-31 21.001011266308495 27.84401320228961 30.658423416216706 20.496552115185192 32-33 20.632382769509242 27.683620264830928 30.87610982952476 20.80788713613507 34-35 20.83920141944452 27.497977981023652 30.77337705927902 20.889443540252806 36-37 21.20230155196824 27.729183643735933 30.308041187231318 20.760473617064513 38-39 21.024342012782714 27.818763390005447 30.421230595010673 20.735664002201165 40-41 20.81574054270089 27.761400200144564 30.491447020234585 20.93141223691996 42-43 20.837608876477184 27.858627313351935 30.224697069233823 21.079066740937055 44-45 21.069491874490513 27.97967507953531 30.01854293982593 20.932290106148248 46-47 21.189298298616972 28.15493729797739 29.575922533224407 21.07984187018123 48-49 21.155446491654576 28.09421532379726 29.516676173260898 21.23366201128726 50-51 21.210429272924127 28.199107979983438 29.194965111403835 21.395497635688592 52-53 21.195206081685303 28.326571103701788 29.091153090251098 21.38706972436181 54-55 21.296562909436343 28.67651315307565 28.9438479005792 21.083076036908807 56-57 21.202129222062858 28.342948828430792 29.016326983284852 21.4385949662215 58-59 21.084442267412975 28.04218101168809 29.25602662378492 21.61735009711402 60-61 21.326228857795602 28.380546658342503 28.989596271536733 21.303628212325165 62-63 21.131251982724383 28.337128090864354 29.077030871799224 21.454589054612036 64-65 21.14512243320345 28.220675577473607 29.206369665163756 21.427832324159187 66-67 21.096954794189053 28.292114938760214 29.029559026720687 21.581371240330046 68-69 21.41294307475949 28.42433781525169 28.809023265045074 21.353695844943747 70-71 21.45109375860612 28.202900411495087 28.823084340942795 21.522921488956 72-73 21.176463241573217 28.371994560898735 28.973443010427026 21.478099187101023 74-75 21.2398921866549 28.47403500267997 28.760398198214965 21.525674612450167 76-77 21.257846365053013 28.519734578385464 28.640863882934525 21.581555173626995 78-79 21.317743942576982 28.571639109796322 28.749534217315542 21.361082730311153 80-81 21.2066402720534 28.497171251898617 28.729891883915577 21.56629659213241 82-83 21.059377428577765 28.450072109928065 28.94169977104044 21.548850690453733 84-85 21.142036772332638 28.506051392341668 28.71249376314112 21.639418072184576 86-87 21.212123672248183 28.602982457345515 28.61218749551145 21.57270637489485 88-89 20.995246078479983 28.492423738108798 28.7495848764771 21.76274530693412 90-91 20.88373078235908 28.443282566717592 28.725362571253754 21.94762407966957 92-93 20.98494045121196 28.58844518713906 28.62997197410227 21.79664238754671 94-95 20.885993227419366 28.37441766228881 28.790961283187155 21.948627827104673 96-97 20.856039909787334 28.472111032817114 28.675955037544064 21.995894019851484 98-99 20.722499943173574 28.429568576259438 28.74511136605986 22.102820114507125 100-101 20.7191881441601 28.487454460311962 28.585151574778195 22.208205820749743 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3526.0 1 2469.5 2 2588.5 3 3517.5 4 2425.0 5 1750.0 6 3090.5 7 6514.0 8 10820.5 9 12910.5 10 10809.5 11 8112.5 12 7815.5 13 8391.5 14 9079.0 15 9820.0 16 10528.0 17 11237.0 18 12182.0 19 13429.0 20 15148.5 21 17774.0 22 21983.0 23 28924.0 24 37950.0 25 49133.5 26 64990.0 27 84450.5 28 108476.5 29 133780.0 30 154072.5 31 173442.5 32 194222.0 33 212695.0 34 228967.5 35 244570.0 36 257357.0 37 267658.0 38 278961.0 39 286220.5 40 289591.5 41 289336.5 42 284407.0 43 279854.0 44 274870.5 45 270208.0 46 264379.5 47 255589.5 48 247016.0 49 242778.0 50 237249.0 51 225415.5 52 208254.5 53 193211.5 54 182309.5 55 171530.5 56 157929.0 57 141135.5 58 127183.0 59 116007.0 60 102901.0 61 87452.5 62 73300.0 63 60228.5 64 49038.0 65 39599.5 66 31223.0 67 24125.0 68 18329.5 69 14004.5 70 10374.5 71 7597.5 72 5765.0 73 4269.5 74 3201.5 75 2312.0 76 1565.0 77 1109.0 78 742.5 79 468.5 80 298.0 81 199.0 82 129.5 83 105.0 84 77.5 85 54.0 86 42.0 87 25.0 88 22.5 89 18.5 90 7.5 91 6.0 92 5.0 93 3.0 94 2.5 95 1.0 96 0.5 97 0.5 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04990701758110827 2 0.001998278982226557 3 2.248063855004877E-4 4 2.1231714186157173E-4 5 0.0025977626768945244 6 0.0017360048658093216 7 0.0 8 1.2489243638915983E-5 9 1.4987092366699182E-4 10-11 4.3712352736205944E-5 12-13 0.0 14-15 5.932390728485092E-4 16-17 0.0 18-19 6.244621819457991E-5 20-21 3.434542000701895E-4 22-23 0.0 24-25 4.745912582788073E-4 26-27 0.007549747779724712 28-29 0.0076746402161138715 30-31 0.0012114566329748506 32-33 0.0067004792122784245 34-35 0.008236656179865092 36-37 0.004739667960968616 38-39 0.0027288997351031423 40-41 0.002216840745907587 42-43 0.001373816800280758 44-45 8.617578110852029E-4 46-47 6.369514255847152E-4 48-49 0.0020857036876989693 50-51 0.001486219993031002 52-53 0.002023257469504389 54-55 9.304486510992408E-4 56-57 2.7476336005615164E-4 58-59 8.055562147100809E-4 60-61 0.006013570812138046 62-63 0.0028725260369506763 64-65 0.0011927227675164764 66-67 0.0056513827466094825 68-69 0.007599704754280376 70-71 0.002909993767867424 72-73 6.556852910430891E-4 74-75 0.003428297378882437 76-77 0.0019608112513098096 78-79 0.0025665395677972346 80-81 9.928948692938208E-4 82-83 2.185617636810297E-4 84-85 6.86908400140379E-4 86-87 0.005058143673760973 88-89 0.002959950742423088 90-91 0.001486219993031002 92-93 1.2489243638915982E-4 94-95 5.682605855706772E-4 96-97 3.9965579644531146E-4 98-99 6.494406692236311E-4 100-101 6.2446218194579915E-6 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8006890.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.74622340239009 #Duplication Level Percentage of deduplicated Percentage of total 1 69.80379689534547 36.12082868481611 2 17.179605087345863 17.779593656292725 3 5.81407262028231 9.02568902060544 4 2.5415709165348908 5.260667857601272 5 1.2579006779935338 3.254580475073568 6 0.7828349723258669 2.4305252019106907 7 0.49233597967376613 1.7833569315263305 8 0.3601314072169538 1.490835220165249 9 0.26542385862034157 1.236121405604334 >10 1.3716823540382812 12.772716729470822 >50 0.08713608615520478 3.0756253553412067 >100 0.04108826203123068 3.6758678172519934 >500 0.0017189753108220864 0.5930818846610829 >1k 6.050923664336688E-4 0.5313392585996999 >5k 2.4203689759608925E-5 0.07820294911918127 >10k+ 7.261106927882676E-5 0.8909675519603328 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29521 0.36869496146443875 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25761 0.32173540538211465 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15856 0.19802944713865184 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.2489243638915983E-5 0.0 3 0.0 0.0 0.0 1.2489243638915983E-5 0.0 4 0.0 0.0 0.0 2.4978487277831966E-5 0.0 5 0.0 2.4978487277831966E-5 0.0 2.4978487277831966E-5 0.0 6 0.0 2.4978487277831966E-5 0.0 2.4978487277831966E-5 0.0 7 0.0 2.4978487277831966E-5 0.0 2.4978487277831966E-5 0.0 8 0.0 2.4978487277831966E-5 0.0 2.4978487277831966E-5 0.0 9 0.0 2.4978487277831966E-5 0.0 3.746773091674795E-5 0.0 10-11 0.0 2.4978487277831966E-5 0.0 3.746773091674795E-5 0.0 12-13 0.0 3.1223109097289954E-5 0.0 3.746773091674795E-5 0.0 14-15 0.0 4.995697455566393E-5 0.0 4.3712352736205944E-5 0.0 16-17 0.0 8.742470547241189E-5 0.0 6.244621819457991E-5 0.0 18-19 0.0 1.623601673059078E-4 0.0 9.991394911132786E-5 0.0 20-21 0.0 1.8733865458373975E-4 0.0 1.1240319275024385E-4 0.0 22-23 0.0 2.0607252004211372E-4 0.0 1.623601673059078E-4 0.0 24-25 0.0 2.310510073199457E-4 0.0 2.9974184733398365E-4 0.0 26-27 0.0 2.3729562913940368E-4 0.0 4.683466364593494E-4 0.0 28-29 0.0 2.497848727783197E-4 0.0 0.0010490964656689424 0.0 30-31 0.0 2.6227411641723566E-4 0.0 0.0024728702405053646 0.0 32-33 0.0 2.934972255145256E-4 0.0 0.005532734932039781 0.0 34-35 0.0 3.1223109097289957E-4 0.0 0.01029738138028623 0.0 36-37 0.0 3.372095782507316E-4 0.0 0.018465346720137284 0.0 38-39 0.0 3.746773091674795E-4 0.0 0.030167768009801556 0.0 40-41 0.0 3.9341117462585347E-4 0.0 0.04363117265255299 0.0 42-43 0.0 3.9965579644531146E-4 0.0 0.05784393191363938 0.0 44-45 0.0 4.1214504008422743E-4 0.0 0.0749354618334959 0.0 46-47 0.0 4.4336814918151743E-4 0.0 0.09684159517615454 0.0 48-49 0.0 4.7459125827880737E-4 0.0 0.121076972457471 0.0 50-51 0.0 5.120589891955554E-4 0.0 0.1467049004045266 0.0 52-53 0.0 5.245482328344713E-4 0.0 0.17201435263878984 0.0 54-55 0.0 5.245482328344713E-4 0.0 0.2020509835903828 0.0 56-57 0.0 5.307928546539293E-4 0.0 0.23791384669953003 0.0 58-59 0.0 5.370374764733873E-4 0.0 0.27892852280973013 0.0 60-61 0.0 5.432820982928453E-4 0.0 0.32378988596071634 0.0 62-63 0.0 5.495267201123033E-4 0.0 0.36890727860630035 0.0 64-65 0.0 5.557713419317613E-4 0.0 0.4196323416457576 0.0 66-67 0.0 5.807498292095932E-4 0.0 0.48079841236734866 0.0 68-69 0.0 5.869944510290512E-4 0.0 0.5497515264978038 0.0 70-71 0.0 5.994836946679672E-4 0.0 0.6292268283940456 0.0 72-73 0.0 6.119729383068832E-4 0.0 0.7146032979096753 0.0 74-75 0.0 6.244621819457991E-4 0.0 0.8029896751422837 0.0 76-77 0.0 6.556852910430892E-4 0.0 0.9002559046021614 0.0 78-79 0.0 6.619299128625472E-4 0.0 1.0166618999386778 0.0 80-81 0.0 6.619299128625472E-4 0.0 1.1490291486457287 0.0 82-83 0.0 6.619299128625472E-4 0.0 1.2909506687365506 0.0 84-85 0.0 6.619299128625472E-4 0.0 1.4357384702425036 0.0 86-87 0.0 6.619299128625472E-4 0.0 1.5931966094201369 0.0 88-89 0.0 6.619299128625472E-4 0.0 1.776107827133881 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 14710 0.0 57.504128 1 GTATCAA 27340 0.0 41.12519 1 ATCAACG 33880 0.0 32.83296 3 TCAACGC 34040 0.0 32.622818 4 TATCAAC 35570 0.0 31.353315 2 CAACGCA 35445 0.0 31.329689 5 TATAACG 825 0.0 30.513441 2 AACGCAG 37200 0.0 30.106619 6 GTGGTAT 5960 0.0 28.62463 1 ACGCAGA 42865 0.0 25.939062 7 CGCAGAG 43165 0.0 25.648752 8 ATAACGC 1025 0.0 25.486216 3 TGGTATC 6520 0.0 25.27849 2 GCAGAGT 46670 0.0 23.26481 9 TACCTGG 5865 0.0 21.217907 2 AGAGTAC 43350 0.0 21.047445 10-11 GTACATG 23275 0.0 20.887077 1 GTACCTG 6510 0.0 20.877415 1 GAGTACT 32100 0.0 20.870321 12-13 CAGAGTA 45365 0.0 20.667473 10-11 >>END_MODULE