FastQCFastQC Report
Wed 25 May 2016
SRR2049410_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049410_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11339856
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT455890.4020245054258184No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT384980.3394928471754844No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195730.17260360272652492No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA223550.059.819421
GTATCAA424900.043.099891
ATCAACG519250.034.936633
TCAACGC521200.034.7524644
GTGGTAT83950.034.5181581
TATCAAC538750.033.6978072
CAACGCA546700.033.243425
AACGCAG565750.032.115936
TGGTATC96350.029.1362782
ACGCAGA627000.028.4885467
CGCAGAG634250.027.9381988
GCAGAGT676400.025.2982319
TATAACG9150.023.880042
GTACATG390850.022.813541
GAGTACT441000.022.45980612-13
CAGAGTA647600.022.36985610-11
TACATGG382300.021.917582
GTACTTT454900.021.64007214-15
AGAGTAC625800.020.92871710-11
ACATGGG395750.020.3449523
Produced by FastQC (version 0.11.5)