##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049410_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11339856 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.343295011859055 31.0 31.0 34.0 29.0 34.0 2 31.38431837229679 31.0 31.0 34.0 28.0 34.0 3 31.40190236983609 33.0 31.0 34.0 28.0 34.0 4 35.01773981962381 37.0 35.0 37.0 32.0 37.0 5 35.03534630422115 37.0 35.0 37.0 32.0 37.0 6 35.12376841469592 37.0 35.0 37.0 32.0 37.0 7 35.065482224818375 37.0 35.0 37.0 32.0 37.0 8 35.03856010164503 37.0 35.0 37.0 32.0 37.0 9 36.60280333365785 39.0 37.0 39.0 32.0 39.0 10-11 36.534481125686256 39.0 37.0 39.0 32.0 39.0 12-13 36.48859734197683 39.0 37.0 39.0 32.0 39.0 14-15 37.6392238578691 40.0 38.0 41.0 32.0 41.0 16-17 37.62336413266624 40.0 38.0 41.0 32.0 41.0 18-19 37.651215676812825 40.0 38.0 41.0 32.0 41.0 20-21 37.610293331767174 40.0 38.0 41.0 32.0 41.0 22-23 37.51372089733768 40.0 37.5 41.0 32.0 41.0 24-25 37.402936245398536 40.0 37.0 41.0 32.0 41.0 26-27 37.25703580362925 40.0 37.0 41.0 31.5 41.0 28-29 37.10274879151905 40.0 37.0 41.0 31.0 41.0 30-31 36.91712950323179 40.0 36.5 41.0 31.0 41.0 32-33 36.677155468288134 39.5 36.0 41.0 30.0 41.0 34-35 36.48276358183031 39.0 36.0 41.0 30.0 41.0 36-37 36.30109363822609 39.0 35.0 41.0 30.0 41.0 38-39 36.10613767934972 39.0 35.0 41.0 30.0 41.0 40-41 35.89833380600247 39.0 35.0 40.0 29.0 41.0 42-43 35.66627243767469 39.0 35.0 40.0 28.0 41.0 44-45 35.38545286642088 38.5 35.0 40.0 27.0 41.0 46-47 35.03569873374053 38.0 34.5 40.0 25.5 41.0 48-49 34.743247136471574 38.0 34.0 40.0 24.0 41.0 50-51 33.37302338759858 36.5 32.5 39.0 23.0 40.0 52-53 33.67397615101991 37.5 33.5 39.0 23.5 40.0 54-55 34.719286735210744 38.5 34.5 40.0 25.5 41.0 56-57 34.83247609140716 39.0 34.5 40.0 25.5 41.0 58-59 34.67490746796079 38.0 34.0 40.0 25.0 41.0 60-61 34.41741830760461 38.0 34.0 40.0 24.0 41.0 62-63 34.149358819018516 38.0 34.0 40.0 23.5 41.0 64-65 33.86710505847694 37.0 34.0 40.0 23.0 41.0 66-67 33.53914979167284 37.0 33.0 40.0 22.0 41.0 68-69 33.19342957265066 36.0 33.0 39.0 21.5 41.0 70-71 32.85680250260674 36.0 33.0 39.0 20.5 41.0 72-73 32.59616294951188 35.0 32.5 39.0 20.0 40.0 74-75 32.391773008405046 35.0 33.0 38.5 20.0 40.0 76-77 32.050331371050916 35.0 32.0 37.0 18.5 39.5 78-79 31.67817907035151 35.0 32.0 37.0 17.5 39.0 80-81 31.33798802207012 35.0 32.0 36.5 14.0 39.0 82-83 31.000349651706337 35.0 32.0 36.0 9.5 38.0 84-85 30.68169803038063 35.0 31.0 36.0 7.0 37.0 86-87 30.390909417191892 35.0 31.0 35.0 4.0 37.0 88-89 30.10518810820878 35.0 31.0 35.0 2.0 36.0 90-91 29.842109547070088 34.0 31.0 35.0 2.0 36.0 92-93 29.589985005100594 34.0 31.0 35.0 2.0 36.0 94-95 29.332629223863158 34.0 30.5 35.0 2.0 35.5 96-97 29.070176861152383 34.0 30.0 35.0 2.0 35.0 98-99 28.74877577810512 34.0 30.0 35.0 2.0 35.0 100-101 28.208360714633415 34.0 29.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21149416513901187 1101 2 0.24321800340424105 1101 3 0.22299684295229127 1101 4 0.23956754579502615 1101 5 0.18771709218919597 1101 6 0.2531428840769152 1101 7 0.22554056444321446 1101 8 0.19925794088514692 1101 9 0.28634397622336394 1101 10-11 0.24688987393934525 1101 12-13 0.25323911482092143 1101 14-15 0.36164152870152577 1101 16-17 0.3376087884878558 1101 18-19 0.31302877871387125 1101 20-21 0.2122975789939403 1101 22-23 0.23895478622881683 1101 24-25 0.2392113224890906 1101 26-27 0.16824420165632858 1101 28-29 0.17051352575841605 1101 30-31 0.17155808423652985 1101 32-33 0.08989603614915609 1101 34-35 0.12387337791768971 1101 36-37 0.07475630217643214 1101 38-39 -0.03848104636901439 1101 40-41 -0.06633463638925008 1101 42-43 -0.15354932567652924 1101 44-45 -0.2213951704706929 1101 46-47 -0.2413332858470909 1101 48-49 -0.26599006100613565 1101 50-51 -0.28974445205014376 1101 52-53 -0.22717717724042785 1101 54-55 0.013814745691185237 1101 56-57 0.011210769122627084 1101 58-59 0.007686855883314081 1101 60-61 0.044891025800929185 1101 62-63 0.01989746280093385 1101 64-65 -0.02438311574434948 1101 66-67 -0.04428587212407109 1101 68-69 -0.05468466129840266 1101 70-71 -0.20522359701811865 1101 72-73 -0.21618330903044836 1101 74-75 -0.2218043447725293 1101 76-77 -0.31035335879747095 1101 78-79 -0.28996977600101914 1101 80-81 -0.3562256067330729 1101 82-83 -0.34066992242375704 1101 84-85 -0.47244974837904863 1101 86-87 -0.5167033211553971 1101 88-89 -0.514475648204705 1101 90-91 -0.5163206463660082 1101 92-93 -0.5878178501875624 1101 94-95 -0.7055515874596097 1101 96-97 -0.748091317012257 1101 98-99 -0.9171273253177077 1101 100-101 -0.9984228222854981 1102 1 0.3127532738351704 1102 2 0.32111128894811714 1102 3 0.26178708574754594 1102 4 0.3352745444008036 1102 5 0.2995761624875257 1102 6 0.29491279130400727 1102 7 0.3166702061231632 1102 8 0.2833407823297378 1102 9 0.3938428908899567 1102 10-11 0.3607957675155262 1102 12-13 0.36835542852902137 1102 14-15 0.4561395404030293 1102 16-17 0.3710316848236346 1102 18-19 0.387418594714525 1102 20-21 0.3647864278890083 1102 22-23 0.30711561673181365 1102 24-25 0.2675205417805344 1102 26-27 0.36007725958560144 1102 28-29 0.3407798650642775 1102 30-31 0.2105982193773812 1102 32-33 0.3039735116211375 1102 34-35 0.29376939020487214 1102 36-37 0.24120930574726174 1102 38-39 0.2014213145623316 1102 40-41 0.2570066429376183 1102 42-43 0.17826445906259636 1102 44-45 0.14657354338930872 1102 46-47 0.09233678207728957 1102 48-49 0.03794332060927985 1102 50-51 -0.01885863137346888 1102 52-53 0.15469391601659055 1102 54-55 0.30989412296913343 1102 56-57 0.30307136561043535 1102 58-59 0.25157684807594904 1102 60-61 0.318713735794951 1102 62-63 0.312291176420608 1102 64-65 0.2336939019816313 1102 66-67 0.20761758087922288 1102 68-69 0.19713406102872 1102 70-71 0.2019006941142223 1102 72-73 0.15563556501292197 1102 74-75 0.09605640666222115 1102 76-77 0.07773293404932247 1102 78-79 0.05217261125357098 1102 80-81 0.04502150877752342 1102 82-83 -0.03413795656168617 1102 84-85 -0.06565493727758209 1102 86-87 -0.1150876879770415 1102 88-89 -0.17846322308708906 1102 90-91 -0.2169846155979549 1102 92-93 -0.26345578020712423 1102 94-95 -0.29990023071010086 1102 96-97 -0.471185338440133 1102 98-99 -0.45310028995668716 1102 100-101 -0.5157062807116262 1103 1 0.26831598649102517 1103 2 0.2613374859430806 1103 3 0.2609748229109705 1103 4 0.31460185956172637 1103 5 0.3348173962444747 1103 6 0.3059038434092187 1103 7 0.31418358450819284 1103 8 0.3106121589071904 1103 9 0.40800396608366185 1103 10-11 0.3902929808466098 1103 12-13 0.35906142856073586 1103 14-15 0.3935465595049621 1103 16-17 0.44461341073871097 1103 18-19 0.4164073258051104 1103 20-21 0.418859709567613 1103 22-23 0.36094648636061066 1103 24-25 0.4032714994109057 1103 26-27 0.34900680169906906 1103 28-29 0.3620568811720304 1103 30-31 0.40401979645758246 1103 32-33 0.26892674442638054 1103 34-35 0.30194403292290417 1103 36-37 0.40136086718036523 1103 38-39 0.33380904095375286 1103 40-41 0.3054523492241472 1103 42-43 0.34818410611668327 1103 44-45 0.31892578424730544 1103 46-47 0.3045196004447632 1103 48-49 0.29046746386788413 1103 50-51 0.19815275481992956 1103 52-53 0.32178251416016934 1103 54-55 0.4889207280586376 1103 56-57 0.562737121382753 1103 58-59 0.5470653916212314 1103 60-61 0.5129106623004276 1103 62-63 0.45258328063354725 1103 64-65 0.40933525362658685 1103 66-67 0.41350196455151433 1103 68-69 0.37717062544048474 1103 70-71 0.3743600677590706 1103 72-73 0.43168282156205606 1103 74-75 0.37204900619440906 1103 76-77 0.2512994164034197 1103 78-79 0.3328139935198813 1103 80-81 0.2991674930975421 1103 82-83 0.27814946099032056 1103 84-85 0.21256781955523607 1103 86-87 0.17373651668366463 1103 88-89 0.11628986268533126 1103 90-91 0.12153191997436963 1103 92-93 0.03125716223052066 1103 94-95 0.003007740715897711 1103 96-97 0.02288116627173764 1103 98-99 -0.03363402768203372 1103 100-101 -0.03590756450973487 1104 1 0.09950467611312064 1104 2 0.12384760939203332 1104 3 0.16071431157823213 1104 4 0.1149015975713894 1104 5 0.14405061317182088 1104 6 0.0526388519595713 1104 7 0.03566844509645506 1104 8 0.08466151439236569 1104 9 0.07901703053630627 1104 10-11 0.11124619158874793 1104 12-13 0.11676781709162043 1104 14-15 0.11755871985790378 1104 16-17 0.08536967049292343 1104 18-19 0.04961467290104338 1104 20-21 0.09303708145005629 1104 22-23 0.04850052067186539 1104 24-25 0.0786820895501279 1104 26-27 0.05650354229539545 1104 28-29 0.04905230300919783 1104 30-31 0.07932760456044008 1104 32-33 0.04170532508207003 1104 34-35 0.11016043888020022 1104 36-37 0.06529023855562599 1104 38-39 0.018890697833413128 1104 40-41 -0.0014853052864580718 1104 42-43 0.018906771848982373 1104 44-45 -0.04672961907581197 1104 46-47 -0.0207560477654809 1104 48-49 -0.13564488163397215 1104 50-51 -0.18332115287581274 1104 52-53 -0.04401526347118789 1104 54-55 0.08743639592127295 1104 56-57 0.025451255125858552 1104 58-59 0.12773948557099146 1104 60-61 -0.04991051598386065 1104 62-63 -0.041821163937562744 1104 64-65 0.008775918271574312 1104 66-67 0.03639492241993736 1104 68-69 0.014048878248893004 1104 70-71 -0.027517902128707306 1104 72-73 -0.09543165294878975 1104 74-75 -0.06590653862225082 1104 76-77 -0.08542820297434872 1104 78-79 -0.0477565207317987 1104 80-81 -0.12493237680881464 1104 82-83 -0.09807151191269625 1104 84-85 -0.07685447145053459 1104 86-87 -0.10666887328276786 1104 88-89 -0.11100288922018464 1104 90-91 -0.10452807066806358 1104 92-93 -0.10894123285747881 1104 94-95 -0.16109852876397923 1104 96-97 -0.195977600824083 1104 98-99 -0.1764659527962209 1104 100-101 -0.1904917887384272 1105 1 0.32948834672881944 1105 2 0.3581794844076285 1105 3 0.3576024231651047 1105 4 0.35529313878398483 1105 5 0.34910588591669267 1105 6 0.3576116143764878 1105 7 0.3635099837934135 1105 8 0.3787887057617638 1105 9 0.4554673006930159 1105 10-11 0.4398314421226388 1105 12-13 0.38851365589210474 1105 14-15 0.5141422969870106 1105 16-17 0.44817029025092836 1105 18-19 0.4810887583438159 1105 20-21 0.47506204072169567 1105 22-23 0.4190475410599319 1105 24-25 0.4435433938398816 1105 26-27 0.4930089861756386 1105 28-29 0.5299681150619904 1105 30-31 0.5458479023883029 1105 32-33 0.46382963241285324 1105 34-35 0.5087562416208868 1105 36-37 0.46640643848117236 1105 38-39 0.467397155722054 1105 40-41 0.4403797667747824 1105 42-43 0.5117992472375121 1105 44-45 0.4109668736776726 1105 46-47 0.4584848974971294 1105 48-49 0.5040498775187032 1105 50-51 0.3667772810540626 1105 52-53 0.42723951524109083 1105 54-55 0.5682992921609653 1105 56-57 0.5644487026129354 1105 58-59 0.6113581439671592 1105 60-61 0.5681097873824754 1105 62-63 0.623073964253301 1105 64-65 0.5598223053519362 1105 66-67 0.5745504050266632 1105 68-69 0.5991010822644967 1105 70-71 0.5170953908024316 1105 72-73 0.5513549179368269 1105 74-75 0.5648394769994027 1105 76-77 0.5553622432076253 1105 78-79 0.6263549025607311 1105 80-81 0.5976385708657332 1105 82-83 0.621241420935057 1105 84-85 0.6008253939222072 1105 86-87 0.5860564965936597 1105 88-89 0.5322110027397322 1105 90-91 0.4997461493094768 1105 92-93 0.5019466544301991 1105 94-95 0.5145681711335826 1105 96-97 0.4631349605612236 1105 98-99 0.4912447456973581 1105 100-101 0.35852785705905177 1106 1 0.24179248122706554 1106 2 0.2773398280988779 1106 3 0.27165799389465306 1106 4 0.26832997810463866 1106 5 0.3112763104163889 1106 6 0.37026101782100795 1106 7 0.35971337640738454 1106 8 0.3486870195123899 1106 9 0.38697098106739247 1106 10-11 0.3882485197442378 1106 12-13 0.4001615534713565 1106 14-15 0.448398097585617 1106 16-17 0.45528714931452896 1106 18-19 0.46420388644669686 1106 20-21 0.45366841843606664 1106 22-23 0.46461864739092107 1106 24-25 0.4630470850224384 1106 26-27 0.4878681183820319 1106 28-29 0.5135807911706536 1106 30-31 0.5334940089613482 1106 32-33 0.5150639795913889 1106 34-35 0.5786749295493507 1106 36-37 0.5768183226201415 1106 38-39 0.56719583827946 1106 40-41 0.5374319696290897 1106 42-43 0.5381501895798309 1106 44-45 0.5444723010749399 1106 46-47 0.5254808497570806 1106 48-49 0.47583458344264784 1106 50-51 0.44767294852587014 1106 52-53 0.5547981032929314 1106 54-55 0.6267547673581717 1106 56-57 0.6387698191704203 1106 58-59 0.6769769721843559 1106 60-61 0.6016797899276511 1106 62-63 0.6370599535440462 1106 64-65 0.66921248958419 1106 66-67 0.6803089230204478 1106 68-69 0.6914700501061688 1106 70-71 0.7349218844691023 1106 72-73 0.6986042838379163 1106 74-75 0.6630021307148581 1106 76-77 0.7142361302367348 1106 78-79 0.7061647415660666 1106 80-81 0.7156499820565685 1106 82-83 0.6993096223788946 1106 84-85 0.7181200798907881 1106 86-87 0.6613330941652542 1106 88-89 0.7198316725514324 1106 90-91 0.6760447920492254 1106 92-93 0.6632110491114602 1106 94-95 0.7202086059618438 1106 96-97 0.6945570166276269 1106 98-99 0.7298765436605734 1106 100-101 0.7252014139431608 1107 1 0.18115180415286858 1107 2 0.20415899263965898 1107 3 0.16792907667890233 1107 4 0.19087344451639154 1107 5 0.19503226574742882 1107 6 0.2309803929688954 1107 7 0.23433814787689045 1107 8 0.2670190058941557 1107 9 0.3375468396533634 1107 10-11 0.25045346082957565 1107 12-13 0.25733207665755486 1107 14-15 0.3958664859173453 1107 16-17 0.3013119915946447 1107 18-19 0.2897273287016162 1107 20-21 0.33640357234171603 1107 22-23 0.35264285484775115 1107 24-25 0.3134103646307551 1107 26-27 0.36436493425914307 1107 28-29 0.3760036235007931 1107 30-31 0.3218987498581001 1107 32-33 0.394749428629531 1107 34-35 0.35004429663318604 1107 36-37 0.355812007055583 1107 38-39 0.4427374467221057 1107 40-41 0.42311538764476353 1107 42-43 0.4030554252090397 1107 44-45 0.4532812760693119 1107 46-47 0.4175104256939477 1107 48-49 0.3742854983886943 1107 50-51 0.4289740183958628 1107 52-53 0.47342731911400904 1107 54-55 0.519751941114329 1107 56-57 0.5016753246804626 1107 58-59 0.5612741975033586 1107 60-61 0.5634480214518547 1107 62-63 0.5537859135487082 1107 64-65 0.5634265109674672 1107 66-67 0.6707576653816787 1107 68-69 0.5795259766538479 1107 70-71 0.6159983970083971 1107 72-73 0.585149929897895 1107 74-75 0.594726033503612 1107 76-77 0.5952612418090553 1107 78-79 0.5487755545754389 1107 80-81 0.5159102537150204 1107 82-83 0.5597604631592112 1107 84-85 0.5723349297522873 1107 86-87 0.5966241843307607 1107 88-89 0.6391932233920343 1107 90-91 0.6080002567092571 1107 92-93 0.6061371032359588 1107 94-95 0.5720570321180922 1107 96-97 0.5647388639192279 1107 98-99 0.6682259324996878 1107 100-101 0.7130044633316501 1108 1 0.18560116705208785 1108 2 0.1848709512317157 1108 3 0.16958307870243416 1108 4 0.23299620122530484 1108 5 0.24033168312201525 1108 6 0.25925828563299547 1108 7 0.25923282050826657 1108 8 0.2669947807381945 1108 9 0.3060923002062026 1108 10-11 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-0.813029910426728 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 118306.0 3 25309.0 4 19891.0 5 17425.0 6 18158.0 7 19394.0 8 22213.0 9 26075.0 10 30539.0 11 35105.0 12 39963.0 13 45670.0 14 52169.0 15 62126.0 16 76079.0 17 94991.0 18 79335.0 19 52579.0 20 48842.0 21 50227.0 22 52930.0 23 58418.0 24 67168.0 25 79865.0 26 96453.0 27 116681.0 28 142382.0 29 174014.0 30 216086.0 31 274028.0 32 352968.0 33 473163.0 34 661441.0 35 987052.0 36 1519363.0 37 2264427.0 38 2324099.0 39 544774.0 40 148.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.567498439785965 20.322118490185556 15.263197258680133 21.84718581134834 2 15.40344159087087 20.44109410513778 40.47887581553403 23.676588488457316 3 17.88183806745126 26.316190961981366 31.005288821583672 24.796682148983702 4 12.74955135719904 17.736111695367548 38.90970605595686 30.604630891476546 5 12.996766007521995 37.917944495828046 35.23120506433063 13.854084432319326 6 29.494970892282442 38.40528105917503 17.951944816730865 14.147803231811656 7 26.249319205735322 33.724255041757736 22.631964736973547 17.3944610155334 8 25.316188899894737 35.51432594812576 20.310564275575196 18.8589208764043 9 25.37100227830813 17.10754261718541 21.71519657893068 35.80625852557578 10-11 23.85626605653824 27.297983553889317 28.510970533776092 20.334779855796352 12-13 24.87594879417395 24.94969514588143 28.717796823774087 21.45655923617053 14-15 22.165085098661887 26.561320906549106 26.776096974421677 24.497497020367327 16-17 21.006028532180267 29.97946541285262 27.427883446231892 21.586622608735222 18-19 20.988283504997355 28.248007522092443 30.350425128388647 20.41328384452155 20-21 21.850540109480484 27.271220904751065 30.38280073137492 20.495438254393534 22-23 21.72864518186314 27.088990431523275 30.402687505247034 20.77967688136655 24-25 21.375333225005118 27.372411008997933 30.271318077281524 20.980937688715425 26-27 21.1506384057964 27.545825381572104 30.23470750761195 21.068828705019545 28-29 21.228889501384355 27.389263262166818 30.459727720143093 20.922119516305738 30-31 21.41037382069076 27.55887562341294 30.184020081491774 20.84673047440452 32-33 21.18619383291379 27.558981861537085 30.270253705670736 20.984570599878392 34-35 21.514318276387794 27.37101553526716 30.111624692489418 21.003041495855626 36-37 21.669112788420946 27.39313910688616 29.85250780905594 21.085240295636957 38-39 21.407729292908627 27.687169092987396 29.899139004623237 21.00596260948074 40-41 21.437361155045394 27.52129562256378 29.93039751093894 21.110945711451887 42-43 21.402211727978624 27.727502414116962 29.702792438853393 21.16749341905102 44-45 21.42787440236617 27.816654705722183 29.526904408352156 21.22856648355949 46-47 21.718165911123137 27.688961106225264 29.220084958903254 21.372788023748345 48-49 21.771183347705954 27.888170720742828 28.932682726876557 21.407963204674658 50-51 21.742176423261604 28.04483271731501 28.662256192423836 21.550734666999553 52-53 21.684983749360466 28.210935603086984 28.603789114139722 21.50029153341283 54-55 21.63360580160306 28.46490874142942 28.578385007057538 21.32310044990998 56-57 21.68234591663897 28.186995014261758 28.57762072046517 21.553038348634104 58-59 21.70685815016684 27.8860487739144 28.756037343127634 21.65105573279113 60-61 21.75532515775755 28.052734324534374 28.726283235966065 21.46565728174201 62-63 21.547425157954226 28.171676448685552 28.780044549196344 21.500853844163885 64-65 21.68619502620877 28.030120411006738 28.75596046234179 21.5277241004427 66-67 21.578358165026387 28.072437815016784 28.742361540990803 21.606842478966033 68-69 21.739598397108832 28.23421248718378 28.53040369158283 21.495785424124563 70-71 21.79105139668923 28.015745173419663 28.559869208813502 21.6333342210776 72-73 21.582786946361416 28.223308345501845 28.544312758353502 21.649591949783236 74-75 21.633990642862187 28.368264710692376 28.419760847864783 21.577983798580657 76-77 21.758397963694936 28.30170891198309 28.262141822594277 21.677751301727696 78-79 21.725271751040882 28.277903090946786 28.413654042936386 21.583171115075938 80-81 21.671540782365025 28.361924940999522 28.321505014743174 21.645029261892283 82-83 21.659518451189257 28.372887644887573 28.426397282200806 21.541196621722364 84-85 21.648669627523223 28.285321165362028 28.27447800825623 21.791531198858518 86-87 21.71188257679714 28.347319005251155 28.254138025670894 21.686660392280814 88-89 21.655543431325306 28.326010071915103 28.332377166768897 21.686069329990694 90-91 21.594398232348187 28.28264068541968 28.304475741063577 21.818485341168557 92-93 21.621208223018975 28.388088717103834 28.26301544059273 21.72768761928446 94-95 21.598349343414704 28.208947655720195 28.398176147051117 21.79452685381398 96-97 21.756866560267298 28.284942431285014 28.208436650603293 21.749754357844402 98-99 21.562814176711502 28.300147453603262 28.257966198554392 21.879072171130844 100-101 21.620711548720944 28.271999723421743 28.187994369304114 21.919294358553195 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4501.0 1 2994.5 2 2059.0 3 2774.5 4 2442.5 5 2327.5 6 3876.5 7 7803.0 8 14414.0 9 18267.5 10 15471.5 11 11864.5 12 11443.5 13 12279.0 14 13133.5 15 13817.5 16 14650.5 17 15720.5 18 16985.5 19 18716.0 20 21067.5 21 24522.0 22 29761.5 23 38656.0 24 50546.5 25 65732.5 26 86272.5 27 110033.0 28 138479.5 29 171482.0 30 197524.5 31 220194.0 32 246330.5 33 269396.5 34 290272.0 35 310493.5 36 330763.0 37 351370.0 38 372122.5 39 387270.5 40 398196.0 41 405215.0 42 409639.0 43 415589.5 44 413848.5 45 410002.5 46 404106.0 47 391773.5 48 378813.0 49 367529.5 50 354897.5 51 334144.5 52 307223.0 53 283102.5 54 264611.0 55 248662.0 56 226574.5 57 199606.0 58 178665.5 59 163984.5 60 148024.0 61 129088.5 62 111610.0 63 94838.5 64 79545.5 65 66118.0 66 53679.5 67 42629.5 68 33523.0 69 26248.5 70 19979.0 71 15222.5 72 11905.5 73 9117.5 74 6900.5 75 5106.5 76 3520.5 77 2511.5 78 1864.5 79 1351.0 80 998.5 81 754.5 82 587.5 83 485.5 84 382.5 85 330.0 86 293.0 87 251.0 88 236.5 89 192.0 90 148.5 91 125.5 92 105.0 93 100.5 94 87.5 95 66.5 96 58.5 97 45.5 98 25.5 99 18.0 100 12.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014709181492251753 2 0.004470956244947026 3 0.004127036533797254 4 0.005793724364753838 5 0.0027513576891981697 6 0.006763754319278833 7 0.0014109526611272665 8 0.0038095721850436195 9 0.010396957421681544 10-11 0.0072531785236073535 12-13 0.00873026959072496 14-15 0.003271646482988849 16-17 0.0061244163947055405 18-19 0.004038851992476801 20-21 0.001040577587581359 22-23 0.0010582144958454498 24-25 0.00757064287236099 26-27 0.001102306766505677 28-29 7.892516448180646E-4 30-31 5.379257020547703E-4 32-33 0.0012434020326184036 34-35 0.001812192324135333 36-37 0.0012081282160902218 38-39 0.0016755062850886291 40-41 0.0028130868681224878 42-43 0.0026984469644058973 44-45 0.007658827413681444 46-47 0.013673013131736418 48-49 0.005198478710840772 50-51 0.00561735528211293 52-53 0.013518690184425624 54-55 0.008121796255613828 56-57 0.005401303155877817 58-59 0.0065388837389116754 60-61 0.0012786758491465852 62-63 0.0022707519390016946 64-65 0.002085564402228741 66-67 0.0024823948381707843 68-69 0.00195328759024806 70-71 0.0022398873495395356 72-73 0.0012037189890241992 74-75 0.0015035464295137435 76-77 0.007821968815124283 78-79 0.0062522839796202 80-81 0.012248832789411081 82-83 0.01509278424699573 84-85 0.008002747124831216 86-87 0.005573263011452703 88-89 0.002685219283207829 90-91 0.0025793978336232843 92-93 9.127100026667006E-4 94-95 0.0011111252206377222 96-97 0.0016755062850886291 98-99 0.00613764407590361 100-101 0.011402261192734723 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.1339856E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.88393448718989 #Duplication Level Percentage of deduplicated Percentage of total 1 65.08730622098398 25.959378572653918 2 16.831829043985678 13.4263913377981 3 6.797039843831105 8.132780755145378 4 3.3966640476606926 5.418893054075693 5 1.9476972618806647 3.884091499686378 6 1.241494479000001 2.9709410679986394 7 0.8633197621648551 2.4102812184975604 8 0.6164645447556876 1.9669625213368944 9 0.4573991759463996 1.641859089114676 >10 2.465765823335441 17.934029680397252 >50 0.18194291400002818 5.026036999209345 >100 0.10600509335196254 7.820690005092898 >500 0.005522717375144339 1.4685213584258037 >1k 0.001438424386834113 0.8915991971001548 >5k 4.4258936580408764E-5 0.13262984429957983 >10k+ 6.638840487061315E-5 0.9149137991677943 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 45589 0.4020245054258184 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 38498 0.3394928471754844 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19573 0.17260360272652492 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 8.818454132045416E-6 0.0 0.0 0.0 6 0.0 8.818454132045416E-6 0.0 0.0 0.0 7 0.0 1.763690826409083E-5 0.0 0.0 0.0 8 0.0 1.763690826409083E-5 0.0 0.0 0.0 9 3.527381652818166E-5 1.763690826409083E-5 0.0 8.818454132045416E-6 0.0 10-11 3.527381652818166E-5 2.204613533011354E-5 0.0 8.818454132045416E-6 0.0 12-13 3.527381652818166E-5 3.0864589462158956E-5 0.0 8.818454132045416E-6 0.0 14-15 3.527381652818166E-5 6.172917892431791E-5 0.0 8.818454132045416E-6 0.0 16-17 3.527381652818166E-5 7.936608718840874E-5 0.0 2.204613533011354E-5 0.0 18-19 3.527381652818166E-5 8.818454132045415E-5 0.0 3.527381652818166E-5 0.0 20-21 3.968304359420437E-5 1.0141222251852227E-4 0.0 4.409227066022708E-5 0.0 22-23 4.409227066022708E-5 1.4991372024477208E-4 0.0 1.05821449584545E-4 0.0 24-25 4.409227066022708E-5 1.5432294731079478E-4 0.0 2.3368903449920352E-4 0.0 26-27 4.8501497726249784E-5 1.5873217437681748E-4 0.0 3.3510125701772576E-4 0.0 28-29 5.73199518582952E-5 1.6314140144284018E-4 0.0 0.0010229406793172682 0.0 30-31 6.172917892431791E-5 1.675506285088629E-4 0.0 0.00244271179457658 0.0 32-33 6.172917892431791E-5 1.675506285088629E-4 0.0 0.005348392431085544 0.0 34-35 7.054763305636333E-5 1.675506285088629E-4 0.0 0.010189723749578477 8.818454132045416E-6 36-37 7.054763305636333E-5 1.675506285088629E-4 0.0 0.018214517009739806 1.763690826409083E-5 38-39 7.054763305636333E-5 1.675506285088629E-4 0.0 0.029788738058049415 1.763690826409083E-5 40-41 7.936608718840874E-5 1.763690826409083E-4 0.0 0.04209489079931879 2.6455362396136246E-5 42-43 8.818454132045415E-5 1.763690826409083E-4 0.0 0.05447159117364453 2.6455362396136246E-5 44-45 8.818454132045415E-5 1.8077830970693104E-4 0.0 0.07134129392824742 2.6455362396136246E-5 46-47 8.818454132045415E-5 1.8518753677295374E-4 0.0 0.09253203920755254 2.6455362396136246E-5 48-49 9.700299545249957E-5 1.8518753677295374E-4 0.0 0.11578630275375631 2.6455362396136246E-5 50-51 1.0582144958454498E-4 1.8518753677295374E-4 0.0 0.139027338618762 2.6455362396136246E-5 52-53 1.0582144958454498E-4 1.8518753677295374E-4 0.0 0.1633750904773394 2.6455362396136246E-5 54-55 1.0582144958454498E-4 1.8518753677295374E-4 0.0 0.19183665118851598 2.6455362396136246E-5 56-57 1.146399037165904E-4 1.9400599090499914E-4 0.0 0.22578769959689082 2.6455362396136246E-5 58-59 1.2345835784863582E-4 1.9400599090499914E-4 0.0 0.2648887252183802 2.6455362396136246E-5 60-61 1.2345835784863582E-4 1.9400599090499914E-4 0.0 0.3066441055336152 2.6455362396136246E-5 62-63 1.2345835784863582E-4 1.9841521797102184E-4 0.0 0.3504233210721547 2.6455362396136246E-5 64-65 1.2345835784863582E-4 2.0282444503704456E-4 0.0 0.3988454527112161 2.6455362396136246E-5 66-67 1.2345835784863582E-4 2.0723367210306726E-4 0.0 0.4570560684368479 2.6455362396136246E-5 68-69 1.2345835784863582E-4 2.1164289916908997E-4 0.0 0.5245260610011273 2.6455362396136246E-5 70-71 1.2345835784863582E-4 2.204613533011354E-4 0.0 0.6005675909817549 2.6455362396136246E-5 72-73 1.2345835784863582E-4 2.4691671569727165E-4 0.0 0.6810271664825374 2.6455362396136246E-5 74-75 1.2345835784863582E-4 2.5573516982931705E-4 0.0 0.7652963141683633 2.6455362396136246E-5 76-77 1.2345835784863582E-4 2.6896285102738515E-4 0.0 0.8589879800942799 2.6455362396136246E-5 78-79 1.2345835784863582E-4 2.910089863574987E-4 0.0 0.970854479986342 2.6455362396136246E-5 80-81 1.2345835784863582E-4 2.9541821342352146E-4 0.0 1.098607424997284 2.6455362396136246E-5 82-83 1.2345835784863582E-4 2.9982744048954416E-4 0.0 1.2331197151004387 2.6455362396136246E-5 84-85 1.2345835784863582E-4 3.0864589462158956E-4 0.0 1.3716620387419383 2.6455362396136246E-5 86-87 1.2345835784863582E-4 3.1746434875363496E-4 0.0 1.5244990765314834 2.6455362396136246E-5 88-89 1.3227681198068122E-4 3.2628280288568036E-4 0.0 1.7018558260351808 2.6455362396136246E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 22355 0.0 59.81942 1 GTATCAA 42490 0.0 43.09989 1 ATCAACG 51925 0.0 34.93663 3 TCAACGC 52120 0.0 34.752464 4 GTGGTAT 8395 0.0 34.518158 1 TATCAAC 53875 0.0 33.697807 2 CAACGCA 54670 0.0 33.24342 5 AACGCAG 56575 0.0 32.11593 6 TGGTATC 9635 0.0 29.136278 2 ACGCAGA 62700 0.0 28.488546 7 CGCAGAG 63425 0.0 27.938198 8 GCAGAGT 67640 0.0 25.298231 9 TATAACG 915 0.0 23.88004 2 GTACATG 39085 0.0 22.81354 1 GAGTACT 44100 0.0 22.459806 12-13 CAGAGTA 64760 0.0 22.369856 10-11 TACATGG 38230 0.0 21.91758 2 GTACTTT 45490 0.0 21.640072 14-15 AGAGTAC 62580 0.0 20.928717 10-11 ACATGGG 39575 0.0 20.344952 3 >>END_MODULE