FastQCFastQC Report
Wed 25 May 2016
SRR2049410_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049410_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11339856
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT446620.39384979844541235No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT381410.3363446590503442No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237210.2091825504662493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA211200.059.5162161
GTATCAA401850.042.561941
ATCAACG507850.033.2958533
TCAACGC513300.032.886814
GTGGTAT79200.032.5820351
TATCAAC531200.031.9845542
CAACGCA532600.031.6861635
AACGCAG555700.030.4707556
TGGTATC91950.027.2748012
ACGCAGA635500.026.3524677
CGCAGAG639950.025.9317238
GCAGAGT688800.023.6239519
GTACATG371250.021.9277691
TACATGG362300.021.2910522
AGAGTAC633800.021.1213310-11
GAGTACT456550.021.06653612-13
CAGAGTA655000.021.0178110-11
AGTACTT459950.020.3893312-13
GTACTTT467350.020.25975814-15
ACATGGG376500.019.4533753
Produced by FastQC (version 0.11.5)