##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049410_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11339856 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07928125365966 33.0 31.0 34.0 30.0 34.0 2 32.27885168912198 34.0 31.0 34.0 30.0 34.0 3 32.34670237435114 34.0 31.0 34.0 30.0 34.0 4 35.977335955588856 37.0 35.0 37.0 35.0 37.0 5 35.8641531250485 37.0 35.0 37.0 35.0 37.0 6 35.88772855669419 37.0 35.0 37.0 35.0 37.0 7 35.818734735255894 37.0 35.0 37.0 35.0 37.0 8 35.81184902171597 37.0 35.0 37.0 35.0 37.0 9 37.42680259784604 39.0 37.0 39.0 34.0 39.0 10-11 37.42045260539464 39.0 37.0 39.0 34.5 39.0 12-13 37.39912746687436 39.0 37.0 39.0 34.0 39.0 14-15 38.6340947363 40.0 38.0 41.0 34.0 41.0 16-17 38.61976161778421 40.0 38.0 41.0 34.0 41.0 18-19 38.65466109093449 40.0 38.0 41.0 34.0 41.0 20-21 38.64251609544248 40.0 38.0 41.0 34.0 41.0 22-23 38.56786122328185 40.0 38.0 41.0 34.0 41.0 24-25 38.471601403051324 40.0 38.0 41.0 34.0 41.0 26-27 38.299267777298056 40.0 38.0 41.0 34.0 41.0 28-29 38.12042758744026 40.0 38.0 41.0 33.0 41.0 30-31 37.940132969942475 40.0 37.0 41.0 33.0 41.0 32-33 37.70653617647349 40.0 37.0 41.0 33.0 41.0 34-35 37.53311378028081 40.0 37.0 41.0 32.5 41.0 36-37 37.35456645128474 39.5 36.0 41.0 32.0 41.0 38-39 37.514717382654595 40.0 37.0 41.0 33.0 41.0 40-41 37.432139526286754 40.0 37.0 41.0 32.5 41.0 42-43 37.24908482083018 40.0 36.5 41.0 32.0 41.0 44-45 37.02111539158875 40.0 36.0 41.0 31.0 41.0 46-47 36.70525481099584 39.5 36.0 41.0 30.5 41.0 48-49 36.43999271242951 39.0 36.0 41.0 30.0 41.0 50-51 36.210284901325025 39.0 35.0 41.0 30.0 41.0 52-53 36.03099854178042 39.0 35.0 40.5 29.5 41.0 54-55 35.91988478513308 39.0 35.0 40.0 29.0 41.0 56-57 35.82792572498275 39.0 35.0 40.0 29.0 41.0 58-59 35.59019863215194 38.5 35.0 40.0 28.0 41.0 60-61 35.343319968084245 38.0 35.0 40.0 28.0 41.0 62-63 35.066698554196805 38.0 34.0 40.0 28.0 41.0 64-65 34.80593285311559 37.5 34.0 40.0 27.0 41.0 66-67 34.46598188724795 37.0 34.0 40.0 26.5 41.0 68-69 34.14727241686314 36.5 34.0 39.0 26.0 41.0 70-71 33.8181451775049 36.0 33.5 39.0 26.0 41.0 72-73 33.44881293025237 35.5 33.0 39.0 26.0 40.0 74-75 33.071938920564776 35.0 33.0 38.0 25.5 40.0 76-77 31.059203088645923 33.5 30.0 35.5 22.5 38.5 78-79 32.373384282833925 35.0 32.5 37.0 25.0 39.0 80-81 32.38786780008494 35.0 33.0 36.5 25.0 39.0 82-83 32.12355346487645 35.0 33.0 36.0 25.0 38.0 84-85 31.863402454140513 35.0 33.0 36.0 24.5 37.0 86-87 31.60589517186109 35.0 32.0 35.0 24.0 37.0 88-89 31.34865438326554 35.0 32.0 35.0 23.5 36.5 90-91 31.09212907994599 35.0 32.0 35.0 22.0 36.0 92-93 30.856581908976622 35.0 32.0 35.0 20.0 36.0 94-95 30.610361366140808 34.0 32.0 35.0 19.0 36.0 96-97 30.373835831777757 34.0 31.5 35.0 16.5 35.0 98-99 30.09760882325137 34.0 31.0 35.0 4.5 35.0 100-101 29.488820713419994 34.0 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3133587584233197 1101 2 0.33507728565046335 1101 3 0.33214015895630666 1101 4 0.25893074301809804 1101 5 0.28241215115517093 1101 6 0.2677696279911572 1101 7 0.2667030002465651 1101 8 0.2608733996902899 1101 9 0.3416249605480317 1101 10-11 0.3262294217783719 1101 12-13 0.2658787036599506 1101 14-15 0.35446466571914215 1101 16-17 0.35259607616997357 1101 18-19 0.33254117322504584 1101 20-21 0.26155049236530914 1101 22-23 0.20250178536966246 1101 24-25 0.2054932270360581 1101 26-27 0.12542932445136756 1101 28-29 0.1825518253785603 1101 30-31 0.13441837230673315 1101 32-33 0.11750880188830592 1101 34-35 0.10777566856705789 1101 36-37 0.07727735555805282 1101 38-39 0.26470657470306236 1101 40-41 0.25837265101438334 1101 42-43 0.3100004228893596 1101 44-45 0.22024829946285962 1101 46-47 0.28411725895345796 1101 48-49 0.2317916628685026 1101 50-51 0.2783494980614165 1101 52-53 0.2139667457198513 1101 54-55 0.2114447414902756 1101 56-57 0.11247308760331265 1101 58-59 0.10984801552936574 1101 60-61 0.1261600987117646 1101 62-63 0.17367691728343004 1101 64-65 0.0534777028978084 1101 66-67 0.08468493293793955 1101 68-69 0.014389684475105469 1101 70-71 -0.03246518073581228 1101 72-73 -0.030948765048272264 1101 74-75 -0.07308392688059229 1101 76-77 -0.07328404507884656 1101 78-79 -0.061119114231495075 1101 80-81 -0.06720047626389913 1101 82-83 -0.05543882815676682 1101 84-85 -0.056960887130824034 1101 86-87 -0.09972061722438852 1101 88-89 -0.12038878879590342 1101 90-91 -0.11955077966820227 1101 92-93 -0.15826098911398034 1101 94-95 -0.2395229501229892 1101 96-97 -0.2729439586952118 1101 98-99 -0.3726011561396483 1101 100-101 -0.4152522034541555 1102 1 0.31633479623493344 1102 2 0.3125980127748349 1102 3 0.31531052691620687 1102 4 0.24341835803877387 1102 5 0.23612769563020208 1102 6 0.25057590070118607 1102 7 0.2429526999220215 1102 8 0.25761463595064527 1102 9 0.3391144208215806 1102 10-11 0.2898993775784291 1102 12-13 0.29265337165751504 1102 14-15 0.36328675198198823 1102 16-17 0.3425348497936582 1102 18-19 0.28655107177612393 1102 20-21 0.32179809693118244 1102 22-23 0.27187280637141953 1102 24-25 0.24144325211437945 1102 26-27 0.24407777225554383 1102 28-29 0.22954644520035572 1102 30-31 0.21178842682211751 1102 32-33 0.1845419079106776 1102 34-35 0.16400611025567002 1102 36-37 0.12773980427325426 1102 38-39 0.2837385866897222 1102 40-41 0.22201684434959645 1102 42-43 0.31563518112071165 1102 44-45 0.24809060723745802 1102 46-47 0.29675286703339054 1102 48-49 0.12496301967628654 1102 50-51 0.19722804541662953 1102 52-53 0.25437269533444606 1102 54-55 0.1991884477957413 1102 56-57 0.20641740229094552 1102 58-59 0.18041379805716673 1102 60-61 0.19809626066211194 1102 62-63 0.15900110965449699 1102 64-65 0.14033115945725427 1102 66-67 0.13279570056183587 1102 68-69 0.10994881120458899 1102 70-71 0.01339453263037882 1102 72-73 -0.03922268528901185 1102 74-75 -0.046267790302017886 1102 76-77 -0.032996423846991974 1102 78-79 0.043833291445857014 1102 80-81 0.07363875390375796 1102 82-83 0.07846929333615549 1102 84-85 0.0257595249495175 1102 86-87 -0.04697789145251363 1102 88-89 0.007893779587938354 1102 90-91 -0.012415964210674701 1102 92-93 -0.015454800497202825 1102 94-95 -0.055955755477956615 1102 96-97 -0.05683692202417845 1102 98-99 -0.12237537923484965 1102 100-101 -0.09459697881124285 1103 1 0.3121204591834399 1103 2 0.3375394819470614 1103 3 0.29753848115733206 1103 4 0.2663086999551183 1103 5 0.271428494209907 1103 6 0.2916097715245485 1103 7 0.24139377944802476 1103 8 0.2837361597664625 1103 9 0.357370032268328 1103 10-11 0.3209926764771396 1103 12-13 0.30716969648781145 1103 14-15 0.39011407700046874 1103 16-17 0.4141067243581986 1103 18-19 0.33754225140040006 1103 20-21 0.30956184640086803 1103 22-23 0.25078304193183953 1103 24-25 0.25022264557165386 1103 26-27 0.24342117495225324 1103 28-29 0.2186353022411751 1103 30-31 0.22127303422324474 1103 32-33 0.28862094892038925 1103 34-35 0.16844377344545336 1103 36-37 0.22928587216859597 1103 38-39 0.2935213118340556 1103 40-41 0.3382856166019934 1103 42-43 0.3886009144849112 1103 44-45 0.3736323220348794 1103 46-47 0.39510034589353893 1103 48-49 0.3068178632234009 1103 50-51 0.3311030886535562 1103 52-53 0.2846952589842786 1103 54-55 0.32332183205839726 1103 56-57 0.31394260387383355 1103 58-59 0.2209162835462095 1103 60-61 0.25218791515224837 1103 62-63 0.2640427098106102 1103 64-65 0.19647406704444847 1103 66-67 0.2197488482984582 1103 68-69 0.23605798580645398 1103 70-71 0.18044109982155732 1103 72-73 0.23315974067212153 1103 74-75 0.23910094153858097 1103 76-77 0.20297029267235445 1103 78-79 0.2664777900430195 1103 80-81 0.23853268195801292 1103 82-83 0.2763389784236452 1103 84-85 0.25233735725474205 1103 86-87 0.29890765163646904 1103 88-89 0.2817953182647628 1103 90-91 0.27544161243897847 1103 92-93 0.29164900932758897 1103 94-95 0.3383656782859177 1103 96-97 0.2803877699942312 1103 98-99 0.23109209463863323 1103 100-101 0.21703228939631813 1104 1 0.32015739956246847 1104 2 0.319244030205887 1104 3 0.2868267151401014 1104 4 0.22363994651676222 1104 5 0.23938185293491898 1104 6 0.28065139509185855 1104 7 0.26025482320334703 1104 8 0.29400530661823154 1104 9 0.3793638924334104 1104 10-11 0.3023898757040442 1104 12-13 0.25665602311185154 1104 14-15 0.3292045864296185 1104 16-17 0.38848882110675476 1104 18-19 0.34344482350216055 1104 20-21 0.32434243500376425 1104 22-23 0.2834513608107301 1104 24-25 0.3019333931576682 1104 26-27 0.31915205119943835 1104 28-29 0.390827078066323 1104 30-31 0.2858614071237824 1104 32-33 0.3497650434359869 1104 34-35 0.3127469689863247 1104 36-37 0.334368414785132 1104 38-39 0.3925638103365685 1104 40-41 0.43221826088300475 1104 42-43 0.48025818716149615 1104 44-45 0.5266427430698144 1104 46-47 0.5658755659006829 1104 48-49 0.5185513923605356 1104 50-51 0.5407075257133513 1104 52-53 0.45812237185516125 1104 54-55 0.4770623212925784 1104 56-57 0.45090627295821406 1104 58-59 0.3824946716330686 1104 60-61 0.4429735596010147 1104 62-63 0.4903973782961657 1104 64-65 0.4821953738436733 1104 66-67 0.4608023450604932 1104 68-69 0.4232427276677484 1104 70-71 0.428458723492561 1104 72-73 0.3792882105733142 1104 74-75 0.3926305905001257 1104 76-77 0.3880039652141072 1104 78-79 0.47857654023173524 1104 80-81 0.4607547564651 1104 82-83 0.45336678615758785 1104 84-85 0.4120894739041283 1104 86-87 0.36461744551818853 1104 88-89 0.36105717706417906 1104 90-91 0.35478248960264835 1104 92-93 0.3145630055239046 1104 94-95 0.26885984550921904 1104 96-97 0.2105264376681646 1104 98-99 0.26580018410005124 1104 100-101 0.23839080369153365 1105 1 0.30501948632597475 1105 2 0.2807221984609356 1105 3 0.2698661602088208 1105 4 0.20405670218142546 1105 5 0.22601671640722998 1105 6 0.22972249842199233 1105 7 0.22738288458061362 1105 8 0.26900941012988966 1105 9 0.28849775179945425 1105 10-11 0.2707868573842731 1105 12-13 0.2757208468849939 1105 14-15 0.30090222738314054 1105 16-17 0.28566631744511284 1105 18-19 0.3390837892180585 1105 20-21 0.30120570062477015 1105 22-23 0.25491378597735803 1105 24-25 0.2732611157290279 1105 26-27 0.32472768017797904 1105 28-29 0.3429824818289333 1105 30-31 0.3364643948979804 1105 32-33 0.33443868381605313 1105 34-35 0.3035169414330312 1105 36-37 0.2300880483979455 1105 38-39 0.3536710382942587 1105 40-41 0.3784787841386361 1105 42-43 0.4192978091632682 1105 44-45 0.41760305654565855 1105 46-47 0.4787839100657578 1105 48-49 0.4781500012281228 1105 50-51 0.4294529685403532 1105 52-53 0.42465522730514493 1105 54-55 0.40754890845051506 1105 56-57 0.3504020771571703 1105 58-59 0.36506745950089226 1105 60-61 0.3774572710746753 1105 62-63 0.41631485580965233 1105 64-65 0.41206805477217756 1105 66-67 0.4798691940159401 1105 68-69 0.43514474643883716 1105 70-71 0.4010127927831135 1105 72-73 0.4568035438700164 1105 74-75 0.4545622727839955 1105 76-77 0.46064442363481817 1105 78-79 0.531748328166735 1105 80-81 0.42227917943639426 1105 82-83 0.528130519550956 1105 84-85 0.5070370356685494 1105 86-87 0.49736888019947756 1105 88-89 0.47562292595030087 1105 90-91 0.5359626494846346 1105 92-93 0.516348802327407 1105 94-95 0.44483642067680407 1105 96-97 0.5148696667583152 1105 98-99 0.5175781841381699 1105 100-101 0.6134590081391735 1106 1 0.28777394757289443 1106 2 0.2501255123853241 1106 3 0.22653981992079508 1106 4 0.18695155723804646 1106 5 0.21041854007815886 1106 6 0.2611308190876116 1106 7 0.22605596980757525 1106 8 0.2030619230439754 1106 9 0.27033582163188186 1106 10-11 0.2683173073792702 1106 12-13 0.24336584186631427 1106 14-15 0.2980753355399699 1106 16-17 0.2907867247455229 1106 18-19 0.3458265087238388 1106 20-21 0.24768427035973417 1106 22-23 0.21813605320866714 1106 24-25 0.2660279598525719 1106 26-27 0.25372421457874594 1106 28-29 0.3337343351510711 1106 30-31 0.3611535563982997 1106 32-33 0.3960188388489456 1106 34-35 0.33190181516904715 1106 36-37 0.3903738333014175 1106 38-39 0.41689825552382587 1106 40-41 0.4140437560745127 1106 42-43 0.42503590043943973 1106 44-45 0.4102506090886351 1106 46-47 0.4277403686200927 1106 48-49 0.41804432645854206 1106 50-51 0.47163394152762095 1106 52-53 0.45616558773782145 1106 54-55 0.47562027879391877 1106 56-57 0.38946049817002404 1106 58-59 0.47413418255025164 1106 60-61 0.45634410615836885 1106 62-63 0.445557906879003 1106 64-65 0.4464043500733865 1106 66-67 0.4590670796348775 1106 68-69 0.5196587688194327 1106 70-71 0.4530293072382747 1106 72-73 0.401596265788875 1106 74-75 0.38880959946595084 1106 76-77 0.4181200956505613 1106 78-79 0.4447825367453291 1106 80-81 0.5075430083392405 1106 82-83 0.46894804385079425 1106 84-85 0.39645818760032014 1106 86-87 0.4203612505971499 1106 88-89 0.47596429569400556 1106 90-91 0.48995949349484036 1106 92-93 0.4676298873970488 1106 94-95 0.5587532000600746 1106 96-97 0.5175582663831655 1106 98-99 0.5533178733743505 1106 100-101 0.5891199004583605 1107 1 0.199008833363564 1107 2 0.20991887549482158 1107 3 0.15594224725899153 1107 4 0.17570239748860672 1107 5 0.17353813289656728 1107 6 0.15932305899842447 1107 7 0.17215232167802696 1107 8 0.18504840781233867 1107 9 0.2257325405638042 1107 10-11 0.17811058955394543 1107 12-13 0.184900052572317 1107 14-15 0.18545785497315848 1107 16-17 0.2018740516480264 1107 18-19 0.17917218339059815 1107 20-21 0.16761413813284776 1107 22-23 0.17445197093886122 1107 24-25 0.2012971166824684 1107 26-27 0.1962401057623211 1107 28-29 0.22177310351738555 1107 30-31 0.26938413439651043 1107 32-33 0.21899660885043915 1107 34-35 0.23578094004881933 1107 36-37 0.22725824307764242 1107 38-39 0.2531233849901042 1107 40-41 0.28758194082722355 1107 42-43 0.2344724292952094 1107 44-45 0.13094795966529915 1107 46-47 0.17281304955797339 1107 48-49 0.09708523268128033 1107 50-51 0.2061868031473324 1107 52-53 0.17396227859271818 1107 54-55 0.23830883484614418 1107 56-57 0.2843750692625804 1107 58-59 0.2747002848118285 1107 60-61 0.35044817391288063 1107 62-63 0.3224993786448138 1107 64-65 0.35353351556772594 1107 66-67 0.32128728796993045 1107 68-69 0.36030310856202163 1107 70-71 0.39231843760395435 1107 72-73 0.41576515644623413 1107 74-75 0.35399912967906033 1107 76-77 0.3618392742004204 1107 78-79 0.40218958825753504 1107 80-81 0.41824081067934316 1107 82-83 0.44499713567384447 1107 84-85 0.3612102356926705 1107 86-87 0.40077679791076193 1107 88-89 0.38840508083825753 1107 90-91 0.4261097361815729 1107 92-93 0.44789016494166844 1107 94-95 0.45752344700838776 1107 96-97 0.5219886750951694 1107 98-99 0.5877266636607601 1107 100-101 0.692919370762052 1108 1 0.15878542605997836 1108 2 0.15628836451478634 1108 3 0.1285628094047766 1108 4 0.1379428204306805 1108 5 0.08250604962755403 1108 6 0.08627053941561513 1108 7 0.13785194418206714 1108 8 0.12731500225240922 1108 9 0.17916791756553607 1108 10-11 0.1035650159966579 1108 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sequence quality scores pass #Quality Count 2 2.0 3 22.0 4 99.0 5 201.0 6 450.0 7 1357.0 8 3383.0 9 6795.0 10 11265.0 11 16741.0 12 22883.0 13 31694.0 14 40808.0 15 52801.0 16 68251.0 17 90795.0 18 78936.0 19 48244.0 20 38775.0 21 38364.0 22 40055.0 23 44288.0 24 52428.0 25 64763.0 26 83212.0 27 105412.0 28 134881.0 29 172359.0 30 218512.0 31 277567.0 32 358404.0 33 475999.0 34 667433.0 35 995904.0 36 1559123.0 37 2411196.0 38 2542475.0 39 583830.0 40 149.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.273333269807594 22.094941339270875 16.660940526847217 22.97078486407432 2 14.35645629319213 21.583478069543048 39.536905859512615 24.523159777752205 3 16.54105474857143 26.707687890311494 31.643991558596912 25.10726580252016 4 12.28474107905655 18.706026685404158 38.306272967712395 30.702959267826895 5 12.782328720855274 37.98708588451197 35.4785956737184 13.75198972091436 6 29.486849127678617 38.96674564632984 17.587905000058203 13.958500225933337 7 26.046627047115944 33.67762341955665 22.574175545086288 17.70157398824112 8 25.37098354688102 35.90292504596178 19.99424860421508 18.73184280294212 9 24.978172092536393 16.91655994118436 21.621237899191005 36.48403006708824 10-11 23.73542209681591 27.541411258401897 28.45585008127532 20.267316563506878 12-13 24.638244965368166 25.039969643353494 28.805647972954862 21.516137418323478 14-15 21.855583054558696 26.680983491338694 26.801765991566874 24.661667462535732 16-17 20.785158118409967 30.071404786798002 27.505962156838677 21.637474937953357 18-19 20.811756030592296 28.247415662008873 30.53318263172487 20.40764567567396 20-21 21.61802974948878 27.31356215852822 30.529353207243716 20.539054884739283 22-23 21.449968147743675 27.123990816109128 30.568558366173253 20.85748266997394 24-25 21.211250851702918 27.370117316634364 30.43789688540295 20.980734946259766 26-27 20.99180415879467 27.539217032921602 30.389084734895743 21.07989407338798 28-29 21.094894936606995 27.400965033253634 30.632836485620963 20.871303544518415 30-31 21.264042720603296 27.523597899508605 30.37059752979073 20.841761850097367 32-33 21.047561320628255 27.500020283818237 30.422905263633194 21.02951313192031 34-35 21.340409603922485 27.349839330070797 30.292167351424137 21.01758371458258 36-37 21.465315874677877 27.382900196069908 30.046983404564802 21.10480052468741 38-39 21.222890032025944 27.61579024787214 30.136620869459087 21.024698850642828 40-41 21.178611029011975 27.516355169342017 30.154563068987244 21.150470732658764 42-43 21.203944377643648 27.69223748278722 29.94046130911044 21.163356830458692 44-45 21.199296528073443 27.788948898018617 29.74615150449445 21.265603069413487 46-47 21.463606103325052 27.735655681685706 29.38669653907049 21.414041675918753 48-49 21.501307915063236 27.895203650036542 29.130871976445405 21.47261645845482 50-51 21.53729952053363 27.9924627569184 28.83711106741013 21.63312665513784 52-53 21.472978347579854 28.181595310597103 28.74658828654601 21.598838055277035 54-55 21.489982957332305 28.401372902140732 28.722552142573566 21.3860919979534 56-57 21.56211405378831 28.1153501046092 28.708705822179798 21.613830019422696 58-59 21.54052392118739 27.87814979136682 28.83511016415781 21.746216123287983 60-61 21.57674592071796 28.054625423387865 28.82955738721702 21.53907126867715 62-63 21.40201425109525 28.09953340738977 28.854536946110603 21.643915395404374 64-65 21.475902771117937 28.07507669379472 28.83380677903699 21.61521375605035 66-67 21.407431819516685 28.09411334549523 28.822887808550167 21.67556702643792 68-69 21.54641992379652 28.188884832577806 28.61601306677975 21.648682176845924 70-71 21.592004460485615 28.00897035580486 28.643548952132015 21.755476231577514 72-73 21.39780743696443 28.21594636513487 28.597152786277842 21.78909341162286 74-75 21.43887205265539 28.36862548768282 28.46342272481891 21.729079734842884 76-77 21.51094587362728 28.304941114304484 28.371142519225813 21.81297049284243 78-79 21.474000475767074 28.298934286160886 28.49634455236778 21.73072068570426 80-81 21.393837114985033 28.377573270107977 28.41417459320546 21.81441502170153 82-83 21.377505872990838 28.336367842373022 28.551878023274675 21.734248261361465 84-85 21.36278846351415 28.322730099061012 28.345358406014793 21.96912303141005 86-87 21.371638581891172 28.38994279981505 28.360015905745144 21.878402712548635 88-89 21.293581200878236 28.328568741390203 28.421279643259247 21.956570414472317 90-91 21.24366722316221 28.320935974264255 28.40294421900518 22.03245258356836 92-93 21.276324436209176 28.396096101498397 28.342933994506446 21.984645467785978 94-95 21.19819480069336 28.23298787311117 28.484041672514433 22.08477565368103 96-97 21.334824009946544 28.357909126752222 28.27313703563294 22.034129827668295 98-99 21.044841126260614 28.3463910401876 28.349605385143338 22.25916244840844 100-101 21.015594996585055 28.386504942082592 28.290454331206178 22.30744573012618 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4219.0 1 2924.5 2 2156.0 3 2776.0 4 2339.5 5 2096.0 6 4004.5 7 9112.0 8 15766.5 9 19075.5 10 16002.5 11 12006.5 12 11496.5 13 12427.5 14 13486.0 15 14235.0 16 15110.5 17 16088.5 18 17330.5 19 18838.0 20 20913.0 21 24508.0 22 29982.0 23 38927.5 24 50721.0 25 65393.5 26 86453.5 27 110364.5 28 139016.5 29 172447.5 30 198468.5 31 222164.0 32 248927.0 33 271897.5 34 292704.5 35 313368.5 36 333540.5 37 354365.5 38 375734.5 39 390749.5 40 401429.0 41 408618.0 42 413335.5 43 419485.0 44 418441.5 45 413564.0 46 405913.5 47 393842.5 48 380651.5 49 370591.0 50 357649.5 51 335566.5 52 306467.5 53 281238.0 54 263739.0 55 245684.0 56 222987.0 57 196641.5 58 176139.5 59 161967.0 60 145889.0 61 125512.0 62 107135.0 63 91029.5 64 75685.5 65 62180.5 66 50118.0 67 39296.0 68 30596.5 69 23588.0 70 17460.0 71 12859.5 72 9852.0 73 7421.0 74 5520.5 75 3986.0 76 2571.0 77 1741.0 78 1189.0 79 739.0 80 470.5 81 315.0 82 226.5 83 157.0 84 102.5 85 71.5 86 48.0 87 38.0 88 21.5 89 15.0 90 13.5 91 9.5 92 6.5 93 2.0 94 3.0 95 2.0 96 1.0 97 0.5 98 2.0 99 2.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0517466888468425 2 0.0023016165284638537 3 3.0864589462158956E-4 4 2.1164289916909E-4 5 0.0028748160470468058 6 0.002010607542106355 7 0.0 8 0.0 9 1.3227681198068122E-4 10-11 4.409227066022708E-5 12-13 0.0 14-15 4.497411607343162E-4 16-17 0.0 18-19 7.054763305636333E-5 20-21 5.687902915169293E-4 22-23 0.0 24-25 6.569748328373834E-4 26-27 0.007702919684341671 28-29 0.007658827413681444 30-31 0.0011331713559678358 32-33 0.00677257277341088 34-35 0.008170297753340078 36-37 0.004700236052380206 38-39 0.0026455362396136245 40-41 0.00216493048941715 42-43 0.001507955656579766 44-45 8.818454132045416E-4 46-47 6.172917892431791E-4 48-49 0.0021472935811530586 50-51 0.0014638633859195389 52-53 0.0021693397164831724 54-55 7.231132388277241E-4 56-57 2.733720780934079E-4 58-59 8.553900508084053E-4 60-61 0.005881908906074293 62-63 0.00270285619147192 64-65 0.0010449868146473818 66-67 0.005414530837075885 68-69 0.007694101230209625 70-71 0.002500031746434875 72-73 5.996548809790883E-4 74-75 0.003443606338563735 76-77 0.0019180137737198778 78-79 0.002411847205114421 80-81 8.994823214686324E-4 82-83 1.8077830970693104E-4 84-85 6.790209681674969E-4 86-87 0.0049118789515492966 88-89 0.0026896285102738517 90-91 0.0012566297138164719 92-93 1.05821449584545E-4 94-95 4.894242043285206E-4 96-97 3.439197111497712E-4 98-99 5.73199518582952E-4 100-101 8.818454132045416E-6 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.1339856E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.85891824799377 #Duplication Level Percentage of deduplicated Percentage of total 1 61.768253241253355 23.384792497819873 2 18.32091079229007 13.872197278281934 3 7.518844791059845 8.539659907922655 4 3.7925039083104033 5.743203816796816 5 2.1419666712248273 4.054627054791404 6 1.3789132478456516 3.1322498352758466 7 0.9261563301478646 2.454429375155096 8 0.656494412491024 1.9883334634209906 9 0.49986278865641004 1.7031838005861495 >10 2.677053472424363 18.468803697813918 >50 0.19692979837423508 5.133865259202474 >100 0.11449720425929441 8.058155873187502 >500 0.005836211116430378 1.4518376968888465 >1k 0.0016602150532759557 0.9354502706995135 >5k 4.676619717301832E-5 0.13619706911413437 >10k+ 7.014929575952747E-5 0.9430131030428371 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 44662 0.39384979844541235 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 38141 0.3363446590503442 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23721 0.2091825504662493 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 8.818454132045416E-6 0.0 0.0 0.0 6 0.0 8.818454132045416E-6 0.0 0.0 0.0 7 0.0 8.818454132045416E-6 0.0 0.0 0.0 8 0.0 8.818454132045416E-6 0.0 0.0 0.0 9 2.6455362396136246E-5 8.818454132045416E-6 0.0 0.0 0.0 10-11 2.6455362396136246E-5 1.3227681198068125E-5 0.0 0.0 0.0 12-13 2.6455362396136246E-5 1.763690826409083E-5 0.0 0.0 0.0 14-15 2.6455362396136246E-5 3.0864589462158956E-5 0.0 0.0 0.0 16-17 2.6455362396136246E-5 4.409227066022708E-5 0.0 1.3227681198068123E-5 0.0 18-19 3.0864589462158956E-5 4.8501497726249784E-5 0.0 2.6455362396136246E-5 0.0 20-21 3.527381652818166E-5 5.291072479227249E-5 0.0 3.527381652818166E-5 0.0 22-23 3.527381652818166E-5 5.291072479227249E-5 0.0 7.936608718840874E-5 0.0 24-25 3.527381652818166E-5 5.291072479227249E-5 0.0 2.0282444503704454E-4 0.0 26-27 3.527381652818166E-5 5.291072479227249E-5 0.0 3.0423666755556686E-4 0.0 28-29 3.527381652818166E-5 5.73199518582952E-5 0.0 9.65620727458973E-4 0.0 30-31 3.527381652818166E-5 6.172917892431791E-5 0.0 0.002380982615652262 0.0 32-33 4.409227066022708E-5 6.172917892431791E-5 0.0 0.005313118614557364 0.0 34-35 4.409227066022708E-5 6.172917892431791E-5 0.0 0.010132403797720183 0.0 36-37 4.409227066022708E-5 6.172917892431791E-5 0.0 0.018130741695485376 0.0 38-39 4.409227066022708E-5 6.172917892431791E-5 0.0 0.02958591361301237 0.0 40-41 4.409227066022708E-5 7.936608718840874E-5 0.0 0.04181270026709334 0.0 42-43 4.409227066022708E-5 7.936608718840874E-5 0.0 0.05410121610009863 0.0 44-45 4.409227066022708E-5 7.936608718840874E-5 0.0 0.07092241735697526 0.0 46-47 5.291072479227249E-5 1.0582144958454498E-4 0.0 0.09201615964082789 0.0 48-49 6.172917892431791E-5 1.0582144958454498E-4 0.0 0.11510728178558881 0.0 50-51 6.172917892431791E-5 1.0582144958454498E-4 0.0 0.13826013310927404 0.0 52-53 6.172917892431791E-5 1.0582144958454498E-4 0.0 0.16254615578892712 0.0 54-55 6.172917892431791E-5 1.0582144958454498E-4 0.0 0.19080048282800063 0.0 56-57 7.054763305636333E-5 1.102306766505677E-4 0.0 0.22445170379588594 0.0 58-59 7.936608718840874E-5 1.2345835784863582E-4 0.0 0.26320880970622557 0.0 60-61 7.936608718840874E-5 1.2345835784863582E-4 0.0 0.3047172733057633 0.0 62-63 8.818454132045415E-5 1.2345835784863582E-4 0.0 0.34817902449554916 0.0 64-65 8.818454132045415E-5 1.2345835784863582E-4 0.0 0.3962969194670549 0.0 66-67 8.818454132045415E-5 1.3668603904670395E-4 0.0 0.45409747707554665 0.0 68-69 8.818454132045415E-5 1.8518753677295374E-4 0.0 0.5210383623919034 0.0 70-71 8.818454132045415E-5 1.8518753677295374E-4 0.0 0.5965816497140705 0.0 72-73 8.818454132045415E-5 2.160521262351127E-4 0.0 0.6762519735700347 0.0 74-75 9.700299545249957E-5 2.3809826156522622E-4 0.0 0.7599214663748817 0.0 76-77 9.700299545249957E-5 2.3809826156522622E-4 0.0 0.8529032467431685 0.0 78-79 9.700299545249957E-5 2.4250748863124895E-4 0.0 0.9638746735408281 0.0 80-81 9.700299545249957E-5 2.4691671569727165E-4 0.0 1.0908956868588102 0.0 82-83 9.700299545249957E-5 2.5132594276329435E-4 0.0 1.2247642298103256 0.0 84-85 1.0582144958454498E-4 2.777813051594306E-4 0.0 1.362539347942337 4.409227066022708E-6 86-87 1.146399037165904E-4 2.910089863574987E-4 0.0 1.5148340508027616 8.818454132045416E-6 88-89 1.2345835784863582E-4 2.9541821342352146E-4 0.0 1.6913442287097826 8.818454132045416E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 21120 0.0 59.516216 1 GTATCAA 40185 0.0 42.56194 1 ATCAACG 50785 0.0 33.295853 3 TCAACGC 51330 0.0 32.88681 4 GTGGTAT 7920 0.0 32.582035 1 TATCAAC 53120 0.0 31.984554 2 CAACGCA 53260 0.0 31.686163 5 AACGCAG 55570 0.0 30.470755 6 TGGTATC 9195 0.0 27.274801 2 ACGCAGA 63550 0.0 26.352467 7 CGCAGAG 63995 0.0 25.931723 8 GCAGAGT 68880 0.0 23.623951 9 GTACATG 37125 0.0 21.927769 1 TACATGG 36230 0.0 21.291052 2 AGAGTAC 63380 0.0 21.12133 10-11 GAGTACT 45655 0.0 21.066536 12-13 CAGAGTA 65500 0.0 21.01781 10-11 AGTACTT 45995 0.0 20.38933 12-13 GTACTTT 46735 0.0 20.259758 14-15 ACATGGG 37650 0.0 19.453375 3 >>END_MODULE