FastQCFastQC Report
Wed 25 May 2016
SRR2049409_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049409_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9784483
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT367960.3760648365376075No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT317110.3240947937668245No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162470.16604863026487962No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA193000.058.949221
GTATCAA370650.042.781121
ATCAACG446550.035.1142273
TCAACGC447650.035.0282974
TATCAAC464350.033.8380662
CAACGCA464700.033.7323575
GTGGTAT76500.033.4701161
AACGCAG486550.032.3836366
TGGTATC82200.030.2792382
ACGCAGA539600.028.7967137
CGCAGAG537400.028.7112858
GCAGAGT567900.026.3746329
TATAACG10150.023.3985772
GAGTACT397900.022.76657512-13
CAGAGTA558000.022.55103710-11
GTACATG293000.021.7902151
AGAGTAC531350.021.66611710-11
GTACTTT431050.020.75471514-15
TACATGG292000.020.6913932
AGTACTT414550.019.96145412-13
Produced by FastQC (version 0.11.5)