##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049409_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9784483 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.413614495523166 31.0 31.0 34.0 30.0 34.0 2 31.45336089806687 31.0 31.0 34.0 29.0 34.0 3 31.472844298467276 33.0 31.0 34.0 28.0 34.0 4 35.092559923707775 37.0 35.0 37.0 32.0 37.0 5 35.11519709319337 37.0 35.0 37.0 32.0 37.0 6 35.201185489309964 37.0 35.0 37.0 32.0 37.0 7 35.14243317710297 37.0 35.0 37.0 32.0 37.0 8 35.11278674611627 37.0 35.0 37.0 32.0 37.0 9 36.6873626332633 39.0 37.0 39.0 33.0 39.0 10-11 36.62772064706945 39.0 37.0 39.0 32.5 39.0 12-13 36.59044570878196 39.0 37.0 39.0 32.0 39.0 14-15 37.75461427037075 40.0 38.0 41.0 32.5 41.0 16-17 37.743184642458885 40.0 38.0 41.0 32.5 41.0 18-19 37.781800377189064 40.0 38.0 41.0 33.0 41.0 20-21 37.74614703709946 40.0 38.0 41.0 32.0 41.0 22-23 37.64689478227925 40.0 38.0 41.0 32.0 41.0 24-25 37.541998999844964 40.0 37.5 41.0 32.0 41.0 26-27 37.400730370730884 40.0 37.0 41.0 32.0 41.0 28-29 37.242368196664046 40.0 37.0 41.0 32.0 41.0 30-31 37.05200295202107 40.0 37.0 41.0 31.0 41.0 32-33 36.798425885148966 40.0 36.0 41.0 30.5 41.0 34-35 36.59702336853158 39.5 36.0 41.0 30.0 41.0 36-37 36.407243131803696 39.0 36.0 41.0 30.0 41.0 38-39 36.20067958623874 39.0 35.0 41.0 30.0 41.0 40-41 35.9865076672932 39.0 35.0 40.5 29.5 41.0 42-43 35.75144808366471 39.0 35.0 40.0 28.5 41.0 44-45 35.47139358308456 39.0 35.0 40.0 27.0 41.0 46-47 35.11000346160344 38.5 35.0 40.0 25.5 41.0 48-49 34.814055632781006 38.0 34.5 40.0 24.0 41.0 50-51 33.46006099658 37.0 32.5 39.0 23.0 40.0 52-53 33.74561430583506 37.5 33.5 39.5 23.5 40.0 54-55 34.793010780436745 39.0 35.0 40.0 24.5 41.0 56-57 34.92224489531026 39.0 35.0 40.5 25.0 41.0 58-59 34.77874528475342 39.0 35.0 40.5 25.0 41.0 60-61 34.53335163441952 38.0 34.0 40.0 24.0 41.0 62-63 34.27313073158797 38.0 34.0 40.0 23.0 41.0 64-65 34.004220815754906 38.0 34.0 40.0 22.5 41.0 66-67 33.69659362686818 37.0 34.0 40.0 22.0 41.0 68-69 33.36025367921841 36.5 33.0 40.0 21.5 41.0 70-71 33.02677785837024 36.0 33.0 39.0 20.0 41.0 72-73 32.770783596844105 36.0 33.0 39.0 19.5 41.0 74-75 32.573389467793035 35.5 33.0 39.0 19.0 40.0 76-77 32.22818901111076 35.0 33.0 38.0 18.0 40.0 78-79 31.85004435083591 35.0 32.0 37.0 16.0 39.0 80-81 31.500356278405306 35.0 32.0 37.0 12.0 39.0 82-83 31.150614958398926 35.0 32.0 36.0 7.5 39.0 84-85 30.81630572611757 35.0 32.0 36.0 7.0 37.0 86-87 30.510967212064244 35.0 31.0 35.5 2.0 37.0 88-89 30.213472035262363 35.0 31.0 35.0 2.0 37.0 90-91 29.94104890365694 35.0 31.0 35.0 2.0 36.0 92-93 29.682669130295388 34.0 31.0 35.0 2.0 36.0 94-95 29.42271318780972 34.0 31.0 35.0 2.0 36.0 96-97 29.16372336688612 34.0 30.5 35.0 2.0 35.5 98-99 28.853354081150737 34.0 30.0 35.0 2.0 35.0 100-101 28.32070820706623 34.0 29.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3261606173375995 1101 2 0.3270474870063964 1101 3 0.3472012192783005 1101 4 0.3176889779345444 1101 5 0.32775769449246894 1101 6 0.33372364502116625 1101 7 0.31796454523796314 1101 8 0.2946318711325091 1101 9 0.3861117720555427 1101 10-11 0.3682297576700009 1101 12-13 0.3346892341344301 1101 14-15 0.45356404417034923 1101 16-17 0.46841733330057167 1101 18-19 0.378399563168756 1101 20-21 0.2879397485292259 1101 22-23 0.3664209121633277 1101 24-25 0.3159139856495514 1101 26-27 0.35656074323633646 1101 28-29 0.3542386383098588 1101 30-31 0.31552310341040624 1101 32-33 0.27980086812829086 1101 34-35 0.3644062215233461 1101 36-37 0.2818776879309368 1101 38-39 0.25482427191725066 1101 40-41 0.22511376306321296 1101 42-43 0.1247246570301499 1101 44-45 0.12075259240523906 1101 46-47 0.1084696427499523 1101 48-49 0.03125562217058331 1101 50-51 -0.013341776236202918 1101 52-53 0.16468407132317253 1101 54-55 0.33537164361418803 1101 56-57 0.37377349886521216 1101 58-59 0.333624995962559 1101 60-61 0.3243400481095762 1101 62-63 0.23022458467549 1101 64-65 0.26071514694127274 1101 66-67 0.2065283476775832 1101 68-69 0.18617911855054814 1101 70-71 0.1586838189978863 1101 72-73 0.17630924346988053 1101 74-75 0.12257055372450765 1101 76-77 0.13915034833911477 1101 78-79 0.08328238203901961 1101 80-81 0.03182712766007256 1101 82-83 -0.025048422014045002 1101 84-85 -0.12319254011718783 1101 86-87 -0.12514332305683595 1101 88-89 -0.18442373734392348 1101 90-91 -0.18759771820764826 1101 92-93 -0.2935891386519458 1101 94-95 -0.3632456210159205 1101 96-97 -0.43046045824052825 1101 98-99 -0.4807237314723629 1101 100-101 -0.6324082651474683 1102 1 0.3691142573617334 1102 2 0.3543465122362015 1102 3 0.3451863330058096 1102 4 0.3656780437328635 1102 5 0.34456016678673507 1102 6 0.39910863063525426 1102 7 0.38581221233352636 1102 8 0.4065977927959423 1102 9 0.4640408764073669 1102 10-11 0.4636934611993766 1102 12-13 0.45510509828035595 1102 14-15 0.49990206348030597 1102 16-17 0.4179180985791717 1102 18-19 0.4914626319597559 1102 20-21 0.4760835316956289 1102 22-23 0.41674190838121916 1102 24-25 0.4629992560081959 1102 26-27 0.4631470169422016 1102 28-29 0.4505314146996753 1102 30-31 0.4032050488333567 1102 32-33 0.3666130999181334 1102 34-35 0.3441751054324129 1102 36-37 0.31034760885064117 1102 38-39 0.3070513214711639 1102 40-41 0.2899968993738966 1102 42-43 0.2400837757422991 1102 44-45 0.31280419285555894 1102 46-47 0.2832047710986956 1102 48-49 0.15602391980273467 1102 50-51 0.08255181794127253 1102 52-53 0.3165560252829849 1102 54-55 0.4356052627199105 1102 56-57 0.43293386862794137 1102 58-59 0.3975603490445607 1102 60-61 0.49914560875117786 1102 62-63 0.48677100494816017 1102 64-65 0.41023246172229477 1102 66-67 0.3675107198165719 1102 68-69 0.30242583349463104 1102 70-71 0.2595470900637622 1102 72-73 0.30156890352678545 1102 74-75 0.25146753548223444 1102 76-77 0.21364175503935456 1102 78-79 0.2152934067243386 1102 80-81 0.16196643667382205 1102 82-83 0.1159676688988256 1102 84-85 0.10043763798778826 1102 86-87 0.08234808687182138 1102 88-89 -3.3740439542739864E-4 1102 90-91 -0.04481916732912339 1102 92-93 -0.03737805691361018 1102 94-95 -0.12385936344777093 1102 96-97 -0.16487999281594057 1102 98-99 -0.23705652688921575 1102 100-101 -0.263766160441687 1103 1 0.2268858353947678 1103 2 0.21027857280048323 1103 3 0.26566576762089156 1103 4 0.2441611894403195 1103 5 0.2513051783609299 1103 6 0.3128069552260655 1103 7 0.309973134829832 1103 8 0.28306609899750157 1103 9 0.37991042623869475 1103 10-11 0.3964255716852918 1103 12-13 0.3400711521826594 1103 14-15 0.35899861703308034 1103 16-17 0.41555571818422266 1103 18-19 0.3987047241185877 1103 20-21 0.38536166229199864 1103 22-23 0.33451660249861703 1103 24-25 0.37552655372973476 1103 26-27 0.40114551299166123 1103 28-29 0.3654461086127867 1103 30-31 0.3935841066657062 1103 32-33 0.3036009990181583 1103 34-35 0.2822909674373264 1103 36-37 0.2450552588779189 1103 38-39 0.22690815245100993 1103 40-41 0.18873221974811827 1103 42-43 0.17063279275787835 1103 44-45 0.18102525823286442 1103 46-47 0.0890309311047659 1103 48-49 0.049234523692319954 1103 50-51 -0.019107711610764966 1103 52-53 0.12326569966548817 1103 54-55 0.28057305609763006 1103 56-57 0.3206765596539469 1103 58-59 0.309929225722577 1103 60-61 0.3446316182069822 1103 62-63 0.2853769780056652 1103 64-65 0.28842965974584445 1103 66-67 0.31289624527908444 1103 68-69 0.2758757364225559 1103 70-71 0.2551752571233479 1103 72-73 0.2866502796172057 1103 74-75 0.20573467319021432 1103 76-77 0.15534030521639863 1103 78-79 0.21184613267539376 1103 80-81 0.20945183209014573 1103 82-83 0.19116927708255815 1103 84-85 0.14403164438054006 1103 86-87 0.11957395534553328 1103 88-89 0.016626673237784217 1103 90-91 -0.006502330993825467 1103 92-93 -0.02248015614918586 1103 94-95 -0.05101631879398383 1103 96-97 -0.1271197791019496 1103 98-99 -0.07901811087254274 1103 100-101 -0.06552773985323057 1104 1 -0.009932024477716794 1104 2 0.027238198735414443 1104 3 0.057090282840832174 1104 4 -0.02220793301378876 1104 5 0.04385364806834957 1104 6 -0.0305466493700024 1104 7 -0.044995502906459706 1104 8 -2.2795111407702962E-4 1104 9 -0.030187789076926208 1104 10-11 -0.0068036346587732055 1104 12-13 -0.026352196054858723 1104 14-15 0.04050295177633245 1104 16-17 0.03624075139084226 1104 18-19 0.022458314275183966 1104 20-21 -0.04942118769277215 1104 22-23 -0.02332283793901979 1104 24-25 -0.018866847767817774 1104 26-27 0.0016638139731242063 1104 28-29 -0.025807503327484937 1104 30-31 0.00989411238340665 1104 32-33 -0.06932647649907153 1104 34-35 -0.008122406782923974 1104 36-37 -0.06777150138800181 1104 38-39 -0.10746218739916458 1104 40-41 -0.11052597879614723 1104 42-43 -0.11439629570539722 1104 44-45 -0.18720370226689909 1104 46-47 -0.09731410898134385 1104 48-49 -0.1530692085353209 1104 50-51 -0.2503343356561274 1104 52-53 -0.19637801696918444 1104 54-55 -0.020347817514753785 1104 56-57 -0.054541245946076344 1104 58-59 0.011138001679377396 1104 60-61 -0.10941718842123294 1104 62-63 -0.08300033967329767 1104 64-65 -0.04600061113515608 1104 66-67 3.2971429696715404E-5 1104 68-69 -0.03491263229990693 1104 70-71 -0.015738271242312862 1104 72-73 -0.034311364730527316 1104 74-75 -0.038683867702090424 1104 76-77 -0.08282432498813819 1104 78-79 -0.12103382020548281 1104 80-81 -0.04846914670625324 1104 82-83 -0.05901205642670604 1104 84-85 0.002284907159669558 1104 86-87 -0.021345598798721 1104 88-89 -0.07716300935129183 1104 90-91 -0.08304899244900454 1104 92-93 -0.11183149337619369 1104 94-95 -0.1538382171517867 1104 96-97 -0.13984250677451726 1104 98-99 -0.19785607541453132 1104 100-101 -0.20882921404767174 1105 1 0.2967847820159655 1105 2 0.31026738397539333 1105 3 0.34079037822167635 1105 4 0.2978755738867207 1105 5 0.3250112276010455 1105 6 0.3172658364364196 1105 7 0.3464847007024403 1105 8 0.3302545818636915 1105 9 0.3931016009398789 1105 10-11 0.3987826736484408 1105 12-13 0.3493627952829996 1105 14-15 0.5242000462293177 1105 16-17 0.5000152130081617 1105 18-19 0.4484723034653584 1105 20-21 0.521578332158775 1105 22-23 0.44105091708144784 1105 24-25 0.42528004521746965 1105 26-27 0.5012812767778456 1105 28-29 0.5146824346425305 1105 30-31 0.5098558302767202 1105 32-33 0.49510964915707945 1105 34-35 0.5054065464934396 1105 36-37 0.5088271415437546 1105 38-39 0.491948008618202 1105 40-41 0.5061551653197611 1105 42-43 0.5537127628792007 1105 44-45 0.569678155258778 1105 46-47 0.5960195325553954 1105 48-49 0.5792049189246313 1105 50-51 0.5828388130147104 1105 52-53 0.5994350398245913 1105 54-55 0.741750098705829 1105 56-57 0.7242034253793292 1105 58-59 0.8553168504910147 1105 60-61 0.7958365440841675 1105 62-63 0.7777637452221384 1105 64-65 0.7170609273513406 1105 66-67 0.7509056331391619 1105 68-69 0.7026149032408071 1105 70-71 0.7047726651120598 1105 72-73 0.7313385956896639 1105 74-75 0.7279190900772861 1105 76-77 0.7413662355527393 1105 78-79 0.6522129890782544 1105 80-81 0.6807139845105326 1105 82-83 0.6852427077490297 1105 84-85 0.6903382944772645 1105 86-87 0.6654459794622376 1105 88-89 0.6551369504200544 1105 90-91 0.6487071505314184 1105 92-93 0.6658856845836922 1105 94-95 0.5868928899797154 1105 96-97 0.6316800261383619 1105 98-99 0.6306287723329866 1105 100-101 0.6339548116078326 1106 1 0.31341685361089944 1106 2 0.33488832638001753 1106 3 0.3035131632599182 1106 4 0.3367857826889846 1106 5 0.3599596910193483 1106 6 0.38360376709333366 1106 7 0.4541608227217182 1106 8 0.4717563414626156 1106 9 0.5301838326568458 1106 10-11 0.5006560012636356 1106 12-13 0.47843827342497036 1106 14-15 0.5269373517471152 1106 16-17 0.5377151170685508 1106 18-19 0.5469991505880074 1106 20-21 0.5412054410622815 1106 22-23 0.5735418811930586 1106 24-25 0.568250757891704 1106 26-27 0.5785456381455987 1106 28-29 0.6072912270320217 1106 30-31 0.6206108227048546 1106 32-33 0.6130239394662951 1106 34-35 0.6177225332456899 1106 36-37 0.5923047658456753 1106 38-39 0.6470219723873072 1106 40-41 0.5929971325119325 1106 42-43 0.6511782128877925 1106 44-45 0.7294274845125059 1106 46-47 0.716245017524578 1106 48-49 0.6056711060779136 1106 50-51 0.5150323977376274 1106 52-53 0.6673517864680889 1106 54-55 0.7118788130661713 1106 56-57 0.7596553151191685 1106 58-59 0.7544049150038816 1106 60-61 0.7942404767283051 1106 62-63 0.778032577362687 1106 64-65 0.7932567222048164 1106 66-67 0.7712467694956118 1106 68-69 0.814782593393673 1106 70-71 0.8052236611209906 1106 72-73 0.8232522210370092 1106 74-75 0.8407152026003715 1106 76-77 0.8069708709936307 1106 78-79 0.7708036063864263 1106 80-81 0.7550517030183741 1106 82-83 0.7522485128449716 1106 84-85 0.7572869073123805 1106 86-87 0.7282529805119005 1106 88-89 0.8118204649056615 1106 90-91 0.768500088476678 1106 92-93 0.7639270085177259 1106 94-95 0.8003367904006566 1106 96-97 0.8180137154432785 1106 98-99 0.846948029961144 1106 100-101 0.8933581521246872 1107 1 0.23204429150476358 1107 2 0.21321786998328918 1107 3 0.18626127716499852 1107 4 0.2237456382204357 1107 5 0.21916255803493812 1107 6 0.20648322309915557 1107 7 0.2085232966743007 1107 8 0.29657633506099756 1107 9 0.3310129475909278 1107 10-11 0.3006704516435903 1107 12-13 0.32513355579824577 1107 14-15 0.40585507522675357 1107 16-17 0.38026447819203213 1107 18-19 0.33735956284873225 1107 20-21 0.41945068170542754 1107 22-23 0.41757852833892883 1107 24-25 0.32864832762159324 1107 26-27 0.367043998191356 1107 28-29 0.3912228689417532 1107 30-31 0.39025694752547935 1107 32-33 0.43248374122843813 1107 34-35 0.45486243379761504 1107 36-37 0.4490557272865985 1107 38-39 0.48287771214397424 1107 40-41 0.4090550694551567 1107 42-43 0.39606270609821337 1107 44-45 0.4136974067175174 1107 46-47 0.5037786688976453 1107 48-49 0.48874236565157503 1107 50-51 0.49209669981923554 1107 52-53 0.5206310275392454 1107 54-55 0.6050514754307912 1107 56-57 0.5509826096004602 1107 58-59 0.5873632807985061 1107 60-61 0.7011541156015326 1107 62-63 0.649012959543505 1107 64-65 0.6560324563084805 1107 66-67 0.6556658218398255 1107 68-69 0.6985778127449507 1107 70-71 0.6939789464071211 1107 72-73 0.6694283869033342 1107 74-75 0.6632606947096278 1107 76-77 0.6685823805742146 1107 78-79 0.6447906291074936 1107 80-81 0.6546430403715817 1107 82-83 0.6750102813837309 1107 84-85 0.6678302176350854 1107 86-87 0.6731386045613554 1107 88-89 0.6839744094484459 1107 90-91 0.690952807225603 1107 92-93 0.6655567475798918 1107 94-95 0.6834109265832282 1107 96-97 0.6792446759066486 1107 98-99 0.7769010286471953 1107 100-101 0.8017190074921174 1108 1 0.1894798378375313 1108 2 0.2039834084067529 1108 3 0.18984907277062746 1108 4 0.2317062851241758 1108 5 0.2431058457455606 1108 6 0.293274699182696 1108 7 0.27340784292270115 1108 8 0.2692922291873856 1108 9 0.27709068256750413 1108 10-11 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sequence quality scores pass #Quality Count 2 96428.0 3 20323.0 4 15671.0 5 13745.0 6 14340.0 7 15747.0 8 17909.0 9 21449.0 10 25853.0 11 30904.0 12 37294.0 13 43685.0 14 49958.0 15 58632.0 16 71061.0 17 89452.0 18 72432.0 19 44973.0 20 40797.0 21 41336.0 22 43222.0 23 47678.0 24 55066.0 25 64885.0 26 78153.0 27 94151.0 28 115543.0 29 141334.0 30 175322.0 31 222778.0 32 287365.0 33 382892.0 34 537727.0 35 804844.0 36 1258460.0 37 1949281.0 38 2141962.0 39 561448.0 40 383.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.97903614856847 20.872694474138427 15.511769959826745 21.63649941746636 2 15.28832011970454 20.77728230218651 40.91637546261329 23.01802211549567 3 17.544925284138586 26.490959751777726 31.535913142645022 24.42820182143867 4 12.316540080084227 18.09187680761623 39.8774870964679 29.714096015831643 5 12.440809117933082 37.80140194915597 35.720431898865876 14.037357034045073 6 28.603658230579303 39.10553781705086 18.3613710049908 13.92943294737904 7 25.393879000648994 33.92132333777921 23.22141992058747 17.463377740984328 8 24.65306835812536 35.64075649576965 21.03787180372974 18.66830334237525 9 24.75314894673954 17.20306681807914 22.605354154933394 35.438430080247926 10-11 23.11646382992489 27.55514578401591 29.203738047234644 20.124652338824554 12-13 24.293036049584565 25.288283273026085 29.363005083123632 21.05567559426572 14-15 21.674656449882985 27.059803384285747 27.548761968577377 23.716778197253888 16-17 20.140208057358787 30.353836309561377 28.13700299221521 21.36895264086462 18-19 20.04089500912656 28.535869344472793 31.336301556845562 20.086934089555083 20-21 21.241556136537163 27.71663991404275 31.178656511179508 19.863147438240585 22-23 20.890097435307535 27.62382364275257 31.29466585523808 20.19141306670181 24-25 20.62653171204491 27.81660876046662 31.197162242721564 20.35969728476691 26-27 20.38807991495421 27.794591714658566 31.2153922122682 20.601936158119017 28-29 20.459621943518115 27.79829056649399 31.329223598640738 20.412863891347165 30-31 20.785597138797336 28.135518958360013 30.892545769461787 20.186338133380865 32-33 20.37673352211794 27.986851033329472 31.24434619677581 20.392069247776778 34-35 20.68630744571177 27.76611680975129 31.094028874616825 20.453546869920118 36-37 21.00142033041973 28.03332834917991 30.562915670474755 20.4023356499256 38-39 20.764682787143148 28.196911880971008 30.68045555200622 20.357949779879625 40-41 20.714019542090963 28.00469715809568 30.685377486633058 20.595905813180295 42-43 20.716519850375445 28.07324608198321 30.474162332563353 20.736071735077992 44-45 20.922871495557317 28.238868962370567 30.207107232379 20.631152309693118 46-47 21.09792847666252 28.443396016677514 29.72936685960284 20.72930864705713 48-49 21.09932730488645 28.372154276550347 29.603603392742734 20.924915025820468 50-51 21.13684565350851 28.46343006387565 29.33549258802558 21.06423169459026 52-53 21.06380677769604 28.594213311279148 29.292882056616172 21.049097854408643 54-55 21.121792034787678 28.970555497749622 29.18069596207731 20.726956505385388 56-57 21.074444172172928 28.622786775371996 29.15805157276834 21.144717479686744 58-59 20.9457895231216 28.323132599253885 29.453274314520584 21.277803563103927 60-61 21.22731940661194 28.629349749743366 29.20464055069133 20.938690292953364 62-63 20.982787844298304 28.660547034376073 29.264574037779912 21.092091083545718 64-65 21.084417912900687 28.480201000700667 29.349956452948412 21.085424633450238 66-67 21.00083660181755 28.53805633752578 29.170467214851826 21.290639845804847 68-69 21.33115855095636 28.78893211698691 28.938488435626393 20.941420896430333 70-71 21.391164398073354 28.4764699046642 29.0063159624691 21.126049734793355 72-73 21.135762223441517 28.64054115832275 29.117241413848273 21.10645520438746 74-75 21.185576877772867 28.75876261981291 28.989049516690844 21.06661098572338 76-77 21.33847108578179 28.82341370257773 28.748865901911614 21.089249309728864 78-79 21.32709663963955 28.833431207545644 28.899826565635202 20.939645587179605 80-81 21.242849248888763 28.77828383608781 28.91508414698692 21.063782768036514 82-83 21.142534250590554 28.817874137158146 29.038016074097467 21.001575538153837 84-85 21.120561075743375 28.780582512562177 28.91242513198955 21.186431279704898 86-87 21.252066338069493 28.86130597318691 28.80948622353558 21.077141465208022 88-89 21.093801981260558 28.798718338541573 28.900801549440807 21.206678130757066 90-91 20.96070080993665 28.722718065881985 28.934395601408063 21.382185522773298 92-93 21.082116485533938 28.88328507027948 28.763425900587976 21.271172543598613 94-95 20.99038310689167 28.655995879131407 28.97191891741348 21.381702096563437 96-97 21.003408217640253 28.735668484171516 28.910949416580227 21.349973881608 98-99 20.977053376610154 28.72823093109901 28.83390011852202 21.460815573768816 100-101 21.013320923204045 28.751530908560884 28.734573623950592 21.500574544284476 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3427.0 1 2233.0 2 1585.0 3 2167.0 4 1799.0 5 1584.0 6 2670.5 7 5276.0 8 9468.5 9 12226.0 10 10997.0 11 9306.0 12 9448.5 13 10165.0 14 11036.5 15 11888.0 16 12775.5 17 13969.0 18 15301.0 19 17163.0 20 20117.0 21 24396.0 22 31149.5 23 41993.0 24 55912.0 25 71890.5 26 92950.5 27 118948.5 28 150591.0 29 181945.5 30 206342.5 31 230391.5 32 254507.0 33 275793.0 34 294895.5 35 312049.0 36 324755.5 37 336129.5 38 349570.5 39 354297.0 40 355793.5 41 353073.5 42 344386.0 43 338226.5 44 328544.5 45 317092.5 46 309344.0 47 300170.0 48 289373.5 49 282143.5 50 273473.0 51 260721.0 52 243664.0 53 227352.0 54 215143.0 55 203330.5 56 186076.5 57 165668.0 58 149211.5 59 134558.5 60 118431.0 61 101011.0 62 84798.0 63 69388.0 64 55725.0 65 44309.0 66 34090.0 67 26055.0 68 19930.0 69 15254.0 70 11498.0 71 8607.0 72 6483.0 73 4761.0 74 3496.5 75 2626.0 76 1954.5 77 1465.5 78 1105.5 79 846.0 80 652.0 81 532.5 82 440.0 83 373.5 84 311.0 85 257.0 86 224.5 87 192.0 88 165.0 89 143.5 90 126.0 91 103.0 92 84.0 93 73.5 94 61.0 95 48.0 96 41.0 97 32.0 98 22.0 99 14.5 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013868898336273874 2 0.004016563777564946 3 0.0035668721587027133 4 0.005457621010737103 5 0.0025346254881325873 6 0.006970220092364615 7 0.0013592951206517503 8 0.004323171699516469 9 0.010761938060498445 10-11 0.007271717882283611 12-13 0.008518590098219803 14-15 0.003280704764881292 16-17 0.006075946986672674 18-19 0.004128986682280505 20-21 8.636123134967888E-4 22-23 9.555946900822455E-4 24-25 0.0071592949775680535 26-27 0.0010884581229279053 28-29 7.358590126836543E-4 30-31 6.745374282933498E-4 32-33 0.0014308369691071055 34-35 0.001839647531709136 36-37 0.0014308369691071055 38-39 0.0018140968715465089 40-41 0.002749251033498653 42-43 0.002657268656913196 44-45 0.007757180425373523 46-47 0.013986431373021958 48-49 0.004921057147321939 50-51 0.005636475631875491 52-53 0.013347664868956286 54-55 0.008063788347325046 56-57 0.005524052727159933 58-59 0.0072563874861860354 60-61 0.0012979735362614458 62-63 0.0024017620552869274 64-65 0.002437532979514605 66-67 0.00264193826081562 68-69 0.0021360351895956075 70-71 0.0023097796787014705 72-73 0.0014052863089444789 74-75 0.0016965638347984252 76-77 0.008457268513829498 78-79 0.0068118059993563275 80-81 0.012724228760988188 82-83 0.01509021989204744 84-85 0.008671894059195565 86-87 0.005810220120981354 88-89 0.0031478413320356327 90-91 0.0026674889209782468 92-93 8.53392049431738E-4 94-95 0.001410396440977004 96-97 0.0019418501723596432 98-99 0.006612510850087837 100-101 0.011007224398059663 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9784483.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.13168862553891 #Duplication Level Percentage of deduplicated Percentage of total 1 70.00846364373089 36.49659427827339 2 16.94076566312897 17.663014412569208 3 5.914847574521391 9.250529760674196 4 2.5869950670238833 5.394576852395769 5 1.3308587519723372 3.4689957031197602 6 0.7925066355562855 2.4788825495096227 7 0.4888152656164192 1.7837935655767683 8 0.3456649618160678 1.4416078526523233 9 0.24690694388201598 1.1584508326146599 >10 1.2174116566575486 11.38366827815704 >50 0.07985279070044904 2.8678269678042825 >100 0.04355351137495842 4.086913045184185 >500 0.00239629163701531 0.8209686489688127 >1k 8.827737507826578E-4 0.7577523826784287 >5k 1.9617157749993287E-5 0.07966642608822266 >10k+ 5.885147324997986E-5 0.866758443733148 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 36796 0.3760648365376075 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31711 0.3240947937668245 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16247 0.16604863026487962 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.0220264065050754E-5 0.0 4 0.0 0.0 0.0 1.0220264065050754E-5 0.0 5 0.0 1.0220264065050754E-5 0.0 3.0660792195152265E-5 0.0 6 0.0 1.0220264065050754E-5 0.0 3.0660792195152265E-5 0.0 7 0.0 1.0220264065050754E-5 0.0 5.110132032525377E-5 0.0 8 0.0 1.0220264065050754E-5 0.0 5.110132032525377E-5 0.0 9 1.0220264065050754E-5 1.0220264065050754E-5 0.0 6.132158439030453E-5 0.0 10-11 1.0220264065050754E-5 1.533039609757613E-5 0.0 8.687224455293142E-5 0.0 12-13 1.0220264065050754E-5 2.0440528130101508E-5 0.0 9.709250861798216E-5 0.0 14-15 1.0220264065050754E-5 2.0440528130101508E-5 0.0 1.379735648781852E-4 0.0 16-17 1.0220264065050754E-5 2.0440528130101508E-5 0.0 1.6352422504081206E-4 0.0 18-19 1.0220264065050754E-5 2.0440528130101508E-5 0.0 1.8396475317091358E-4 0.0 20-21 1.0220264065050754E-5 2.0440528130101508E-5 0.0 2.044052813010151E-4 0.0 22-23 1.0220264065050754E-5 2.0440528130101508E-5 0.0 2.503964695937435E-4 0.0 24-25 1.0220264065050754E-5 2.0440528130101508E-5 0.0 3.2704845008162413E-4 0.0 26-27 1.0220264065050754E-5 2.0440528130101508E-5 0.0 4.854625430899108E-4 0.0 28-29 1.0220264065050754E-5 3.5770924227677644E-5 0.0 0.0010373568026026516 0.0 30-31 1.0220264065050754E-5 4.0881056260203016E-5 0.0 0.002452863375612181 0.0 32-33 1.0220264065050754E-5 4.0881056260203016E-5 0.0 0.0052992069177288156 0.0 34-35 2.0440528130101508E-5 4.0881056260203016E-5 0.0 0.010005638519684688 0.0 36-37 2.0440528130101508E-5 4.0881056260203016E-5 0.0 0.017911012774001447 0.0 38-39 2.0440528130101508E-5 4.5991188292728395E-5 0.0 0.02940369971515102 1.0220264065050754E-5 40-41 2.0440528130101508E-5 5.110132032525377E-5 0.0 0.042500968114513565 1.0220264065050754E-5 42-43 2.5550660162626887E-5 5.110132032525377E-5 0.0 0.05676334661729189 1.0220264065050754E-5 44-45 3.0660792195152265E-5 5.110132032525377E-5 0.0 0.07356546074023533 1.0220264065050754E-5 46-47 3.0660792195152265E-5 6.132158439030453E-5 0.0 0.09521198003001283 1.0220264065050754E-5 48-49 3.0660792195152265E-5 6.132158439030453E-5 0.0 0.1179009662544255 1.0220264065050754E-5 50-51 3.0660792195152265E-5 6.132158439030453E-5 0.0 0.14231206697379922 1.0220264065050754E-5 52-53 3.0660792195152265E-5 6.132158439030453E-5 0.0 0.16699911482292934 1.0220264065050754E-5 54-55 3.0660792195152265E-5 6.132158439030453E-5 0.0 0.19589691146686034 1.0220264065050754E-5 56-57 3.5770924227677644E-5 6.132158439030453E-5 0.0 0.23004792384022743 1.0220264065050754E-5 58-59 4.0881056260203016E-5 6.132158439030453E-5 0.0 0.26972809907278694 1.0220264065050754E-5 60-61 4.0881056260203016E-5 6.132158439030453E-5 0.0 0.312719639862423 1.0220264065050754E-5 62-63 5.110132032525377E-5 6.132158439030453E-5 0.0 0.3571777885453938 2.0440528130101508E-5 64-65 5.110132032525377E-5 6.132158439030453E-5 0.0 0.407875408440078 2.0440528130101508E-5 66-67 5.110132032525377E-5 6.132158439030453E-5 0.0 0.4693145258671306 2.0440528130101508E-5 68-69 5.110132032525377E-5 7.154184845535527E-5 0.0 0.5381428942132149 2.0440528130101508E-5 70-71 5.110132032525377E-5 1.0220264065050755E-4 0.0 0.6140232447641842 2.0440528130101508E-5 72-73 5.110132032525377E-5 1.124229047155583E-4 0.0 0.6959642119057287 2.0440528130101508E-5 74-75 7.154184845535527E-5 1.124229047155583E-4 0.0 0.7820648265217488 2.0440528130101508E-5 76-77 7.154184845535527E-5 1.124229047155583E-4 0.0 0.8770979519306232 2.0440528130101508E-5 78-79 7.154184845535527E-5 1.4308369691071055E-4 0.0 0.9897968037759379 2.0440528130101508E-5 80-81 7.154184845535527E-5 1.4308369691071055E-4 0.0 1.1182399724134633 2.0440528130101508E-5 82-83 7.154184845535527E-5 1.4308369691071055E-4 0.0 1.254731599002216 2.0440528130101508E-5 84-85 7.154184845535527E-5 1.5330396097576132E-4 0.0 1.3949025206543872 2.0440528130101508E-5 86-87 7.154184845535527E-5 1.7374448910586283E-4 0.0 1.5508126489667364 2.0440528130101508E-5 88-89 7.154184845535527E-5 1.8396475317091358E-4 0.0 1.7270253318443092 2.0440528130101508E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 19300 0.0 58.94922 1 GTATCAA 37065 0.0 42.78112 1 ATCAACG 44655 0.0 35.114227 3 TCAACGC 44765 0.0 35.028297 4 TATCAAC 46435 0.0 33.838066 2 CAACGCA 46470 0.0 33.732357 5 GTGGTAT 7650 0.0 33.470116 1 AACGCAG 48655 0.0 32.383636 6 TGGTATC 8220 0.0 30.279238 2 ACGCAGA 53960 0.0 28.796713 7 CGCAGAG 53740 0.0 28.711285 8 GCAGAGT 56790 0.0 26.374632 9 TATAACG 1015 0.0 23.398577 2 GAGTACT 39790 0.0 22.766575 12-13 CAGAGTA 55800 0.0 22.551037 10-11 GTACATG 29300 0.0 21.790215 1 AGAGTAC 53135 0.0 21.666117 10-11 GTACTTT 43105 0.0 20.754715 14-15 TACATGG 29200 0.0 20.691393 2 AGTACTT 41455 0.0 19.961454 12-13 >>END_MODULE