FastQCFastQC Report
Wed 25 May 2016
SRR2049409_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049409_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9784483
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT355040.362860255365562No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310170.31700193050567926No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192330.19656633876312118No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA184450.060.1102521
GTATCAA346600.042.464411
ATCAACG438500.033.275483
TCAACGC442050.033.008424
CAACGCA456200.032.0678865
TATCAAC460300.031.8348222
GTGGTAT71050.031.2367081
AACGCAG480900.030.627766
TATAACG9550.029.8425622
TGGTATC77400.027.8614122
ACGCAGA549750.026.515377
CGCAGAG548950.026.4155738
GCAGAGT588650.024.0853639
ATAACGC13750.021.7625893
GAGTACT410200.021.60638212-13
AGAGTAC550550.021.5506610-11
GTACATG282950.021.1292151
CAGAGTA579050.021.05184410-11
AGTACTT432600.019.9605912-13
GTACTTT447050.019.64486514-15
Produced by FastQC (version 0.11.5)