##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049409_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9784483 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.059728245222566 33.0 31.0 34.0 30.0 34.0 2 32.25614444830657 34.0 31.0 34.0 30.0 34.0 3 32.32342792153658 34.0 31.0 34.0 30.0 34.0 4 35.961462552492556 37.0 35.0 37.0 35.0 37.0 5 35.846294382646484 37.0 35.0 37.0 35.0 37.0 6 35.87274636789701 37.0 35.0 37.0 35.0 37.0 7 35.802001189025525 37.0 35.0 37.0 35.0 37.0 8 35.79320706060811 37.0 35.0 37.0 35.0 37.0 9 37.40324542441333 39.0 37.0 39.0 34.0 39.0 10-11 37.397047140865794 39.0 37.0 39.0 34.0 39.0 12-13 37.3801337791685 39.0 37.0 39.0 34.0 39.0 14-15 38.605276844979954 40.0 38.0 41.0 34.0 41.0 16-17 38.59384721706809 40.0 38.0 41.0 34.0 41.0 18-19 38.6379230767737 40.0 38.0 41.0 34.0 41.0 20-21 38.632807016988025 40.0 38.0 41.0 34.0 41.0 22-23 38.55234149826823 40.0 38.0 41.0 34.0 41.0 24-25 38.45790140368173 40.0 38.0 41.0 34.0 41.0 26-27 38.28219804766384 40.0 38.0 41.0 34.0 41.0 28-29 38.10029528386937 40.0 38.0 41.0 33.0 41.0 30-31 37.91119612553878 40.0 37.0 41.0 33.0 41.0 32-33 37.66090185858568 40.0 37.0 41.0 33.0 41.0 34-35 37.48151465948686 40.0 36.5 41.0 32.5 41.0 36-37 37.288414727686686 39.5 36.0 41.0 32.0 41.0 38-39 37.43106728275781 40.0 37.0 41.0 33.0 41.0 40-41 37.33867604450843 40.0 37.0 41.0 32.0 41.0 42-43 37.14751382367366 40.0 36.5 41.0 31.5 41.0 44-45 36.91108784184101 40.0 36.0 41.0 31.0 41.0 46-47 36.58545193445581 40.0 36.0 41.0 30.5 41.0 48-49 36.3154497279008 39.0 36.0 41.0 30.0 41.0 50-51 36.092184175699416 39.0 35.5 41.0 29.5 41.0 52-53 35.91761128308977 39.0 35.0 40.5 29.0 41.0 54-55 35.81785818422905 39.0 35.0 40.0 29.0 41.0 56-57 35.73799545668381 39.0 35.0 40.0 28.5 41.0 58-59 35.51240070630201 39.0 35.0 40.0 28.0 41.0 60-61 35.277025316513914 38.0 35.0 40.0 28.0 41.0 62-63 35.00835501477186 38.0 34.0 40.0 27.0 41.0 64-65 34.760858238498656 38.0 34.0 40.0 26.5 41.0 66-67 34.432660315317634 37.0 34.0 40.0 26.0 41.0 68-69 34.122785690363 37.0 34.0 39.5 26.0 41.0 70-71 33.7974348772439 36.0 33.5 39.0 26.0 41.0 72-73 33.43442428179394 36.0 33.0 39.0 25.5 40.5 74-75 33.062819261886396 35.0 33.0 38.5 25.0 40.0 76-77 31.04309410113953 33.5 30.0 36.0 22.0 38.5 78-79 32.364082394542464 35.0 32.5 37.0 24.5 39.0 80-81 32.37350506919988 35.0 33.0 37.0 25.0 39.0 82-83 32.095222558003314 35.0 33.0 36.0 24.0 39.0 84-85 31.821964430823783 35.0 33.0 36.0 24.0 37.0 86-87 31.547436333631524 35.0 32.0 35.5 23.0 37.0 88-89 31.27661328656813 35.0 32.0 35.0 22.0 37.0 90-91 31.009221386556653 35.0 32.0 35.0 20.0 36.0 92-93 30.76420629480372 35.0 32.0 35.0 19.5 36.0 94-95 30.508250665875753 34.0 32.0 35.0 18.0 36.0 96-97 30.26967975722376 34.0 31.5 35.0 11.0 35.5 98-99 29.988336685750284 34.0 31.0 35.0 2.0 35.0 100-101 29.38330165221811 34.0 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3589531561027144 1101 2 0.40578724030336843 1101 3 0.37765081042478243 1101 4 0.27451992356232324 1101 5 0.3019425794880206 1101 6 0.3075928226930742 1101 7 0.3056283098532333 1101 8 0.31018546616584075 1101 9 0.34780388418825936 1101 10-11 0.3735944349608573 1101 12-13 0.34610321313787296 1101 14-15 0.4562406546067024 1101 16-17 0.4234565999984241 1101 18-19 0.37236487919881966 1101 20-21 0.37726820689969287 1101 22-23 0.29367971985163166 1101 24-25 0.27611481419534556 1101 26-27 0.25784125300376104 1101 28-29 0.24467682793962098 1101 30-31 0.24068526451697636 1101 32-33 0.23234769392941956 1101 34-35 0.16913441148502528 1101 36-37 0.14846040492203372 1101 38-39 0.33041957065626804 1101 40-41 0.36059905881587184 1101 42-43 0.369652091233462 1101 44-45 0.3410202516430729 1101 46-47 0.43364562188167355 1101 48-49 0.3259533326105455 1101 50-51 0.33853144279137837 1101 52-53 0.33126146687295943 1101 54-55 0.3037261841061465 1101 56-57 0.3129988542824478 1101 58-59 0.2867830262543336 1101 60-61 0.3049342587075685 1101 62-63 0.24936754289314678 1101 64-65 0.2070139871907415 1101 66-67 0.19434467525542942 1101 68-69 0.14675313072423535 1101 70-71 0.11206148581564435 1101 72-73 0.09891785592539293 1101 74-75 0.07894226048144759 1101 76-77 0.04496689233549844 1101 78-79 0.14677418062952086 1101 80-81 0.13632760531152144 1101 82-83 0.1220257109039764 1101 84-85 0.07768292538428412 1101 86-87 0.04368187648945465 1101 88-89 -0.009516091432317353 1101 90-91 0.022284605344449204 1101 92-93 0.045370683721241534 1101 94-95 -0.012998661189062233 1101 96-97 -0.056044398453170885 1101 98-99 -0.058624167896052626 1101 100-101 -0.04043889652232835 1102 1 0.3661875979726972 1102 2 0.36337633742835607 1102 3 0.3646532741933868 1102 4 0.2959890239139753 1102 5 0.3080400350725725 1102 6 0.30504859612167934 1102 7 0.2769721397724467 1102 8 0.2839473616629036 1102 9 0.40265678227066815 1102 10-11 0.37087945894484164 1102 12-13 0.29795089793485374 1102 14-15 0.3659431443849641 1102 16-17 0.3901266147795397 1102 18-19 0.3920917466324383 1102 20-21 0.36445149520313436 1102 22-23 0.2926956168835275 1102 24-25 0.3187685020374502 1102 26-27 0.3085266325665046 1102 28-29 0.2799616881306477 1102 30-31 0.28285108739071774 1102 32-33 0.3117051605683585 1102 34-35 0.2609497354216401 1102 36-37 0.23211045846222333 1102 38-39 0.33015855718806364 1102 40-41 0.28009106079746715 1102 42-43 0.33154874788697697 1102 44-45 0.36423871159784227 1102 46-47 0.3419254210721121 1102 48-49 0.19001923799790887 1102 50-51 0.22883300940365814 1102 52-53 0.21504244607334044 1102 54-55 0.2041737783623745 1102 56-57 0.17269926974512373 1102 58-59 0.20388335006060032 1102 60-61 0.30141152949070005 1102 62-63 0.2389652675457583 1102 64-65 0.17191167006493657 1102 66-67 0.14693909169862707 1102 68-69 0.16012918836987922 1102 70-71 0.12613433340685987 1102 72-73 0.0812367808584682 1102 74-75 0.03699756799395004 1102 76-77 0.1074992108640167 1102 78-79 0.12507658711451342 1102 80-81 0.16462953851434747 1102 82-83 0.211992719732784 1102 84-85 0.23387163548085965 1102 86-87 0.1469905998089409 1102 88-89 0.11030812608017371 1102 90-91 0.09473081814384088 1102 92-93 0.10171652898792516 1102 94-95 0.043282906649153574 1102 96-97 -0.03311290561865121 1102 98-99 0.015531872817280856 1102 100-101 0.006059874009192612 1103 1 0.31913638416627776 1103 2 0.34037600410291446 1103 3 0.31663939628371196 1103 4 0.2709615440719375 1103 5 0.26558220154502266 1103 6 0.2551834118111884 1103 7 0.22717628284089386 1103 8 0.25867225080940415 1103 9 0.3468395829102704 1103 10-11 0.279112387053992 1103 12-13 0.2784555621333098 1103 14-15 0.3410102984769168 1103 16-17 0.3563592594891034 1103 18-19 0.33041271490913715 1103 20-21 0.29027272225481937 1103 22-23 0.28580807342017067 1103 24-25 0.34424973946043025 1103 26-27 0.28171630671386083 1103 28-29 0.28796691751059456 1103 30-31 0.3319372558987723 1103 32-33 0.2862373931897153 1103 34-35 0.23124696601224315 1103 36-37 0.2136521099010693 1103 38-39 0.2853003860762513 1103 40-41 0.32771817715130425 1103 42-43 0.3640393829813746 1103 44-45 0.29660526474606996 1103 46-47 0.32109273401543703 1103 48-49 0.24243666794770036 1103 50-51 0.304050281536135 1103 52-53 0.20807312019788782 1103 54-55 0.20520136617623308 1103 56-57 0.21495766943323247 1103 58-59 0.16642038660305047 1103 60-61 0.143049552224376 1103 62-63 0.17086295953506436 1103 64-65 0.1688115509893251 1103 66-67 0.12419836334478163 1103 68-69 0.16611805572713223 1103 70-71 0.0823618753591191 1103 72-73 0.12225389058492198 1103 74-75 0.11490672910221633 1103 76-77 0.12290032985887578 1103 78-79 0.19788454515749976 1103 80-81 0.1550299221547391 1103 82-83 0.24685781491260883 1103 84-85 0.24180689252683862 1103 86-87 0.21705780560303012 1103 88-89 0.17930475939378354 1103 90-91 0.22350815321462036 1103 92-93 0.11461431848175252 1103 94-95 0.1439196221118486 1103 96-97 0.18254999533535354 1103 98-99 0.1208981809460461 1103 100-101 0.09600548717206081 1104 1 0.2928550478355092 1104 2 0.28998233165797416 1104 3 0.2728566222305915 1104 4 0.22521885156029242 1104 5 0.24321653211411132 1104 6 0.2555052500045605 1104 7 0.2526415198347962 1104 8 0.2849214302910923 1104 9 0.3643463005797756 1104 10-11 0.32246154674365357 1104 12-13 0.24412839544631026 1104 14-15 0.35100967816092776 1104 16-17 0.380186245060429 1104 18-19 0.3218677272698116 1104 20-21 0.3376121527342022 1104 22-23 0.32777629871893765 1104 24-25 0.2554913693438081 1104 26-27 0.3181893466190644 1104 28-29 0.35374890257985925 1104 30-31 0.2719766615240289 1104 32-33 0.2784827379152901 1104 34-35 0.2467756536559591 1104 36-37 0.29013992432243896 1104 38-39 0.32522511622842387 1104 40-41 0.3660738709052467 1104 42-43 0.3362749260918818 1104 44-45 0.3152890786894531 1104 46-47 0.3389152319428703 1104 48-49 0.28591279532533775 1104 50-51 0.2796197741466955 1104 52-53 0.2536526581931753 1104 54-55 0.2777392977730102 1104 56-57 0.24486548923205476 1104 58-59 0.27744489032951236 1104 60-61 0.30675482204730997 1104 62-63 0.3299071303904242 1104 64-65 0.35931241258541746 1104 66-67 0.3338765168528681 1104 68-69 0.3056115677197653 1104 70-71 0.2552519555728239 1104 72-73 0.21914300069910553 1104 74-75 0.29095589816923706 1104 76-77 0.28179142892603437 1104 78-79 0.36254014322925343 1104 80-81 0.3211422566092068 1104 82-83 0.34148106526882316 1104 84-85 0.30611349024092505 1104 86-87 0.29996469243830504 1104 88-89 0.29884077651699315 1104 90-91 0.28410199374652123 1104 92-93 0.25479401614098407 1104 94-95 0.2136858926476286 1104 96-97 0.13855191043131398 1104 98-99 0.13961291214383564 1104 100-101 0.18829250095012995 1105 1 0.3254535593752905 1105 2 0.33373302106031844 1105 3 0.3107658027988549 1105 4 0.23361504216625661 1105 5 0.25642337228307355 1105 6 0.21649691877998833 1105 7 0.26034595601723254 1105 8 0.2966514895310297 1105 9 0.271240567347661 1105 10-11 0.292885496743331 1105 12-13 0.24519258934842725 1105 14-15 0.2999586234564404 1105 16-17 0.32332194701800887 1105 18-19 0.3226655685483095 1105 20-21 0.29904037540628536 1105 22-23 0.20975475367907137 1105 24-25 0.24362659277505117 1105 26-27 0.26955618815060234 1105 28-29 0.27336917165764163 1105 30-31 0.32369359778756035 1105 32-33 0.38035588463957737 1105 34-35 0.3422610415704881 1105 36-37 0.2177522725728238 1105 38-39 0.3112487408301945 1105 40-41 0.2741452313474966 1105 42-43 0.32172022323241123 1105 44-45 0.34655436720027666 1105 46-47 0.36503206374167974 1105 48-49 0.3544950677901184 1105 50-51 0.34506826686985903 1105 52-53 0.38359976886739844 1105 54-55 0.3343438127906282 1105 56-57 0.3234438118269338 1105 58-59 0.3598229958843078 1105 60-61 0.3819997626451581 1105 62-63 0.3890272340116496 1105 64-65 0.404045743647778 1105 66-67 0.41879458341182385 1105 68-69 0.354927396722033 1105 70-71 0.29182454720656636 1105 72-73 0.3396676034411428 1105 74-75 0.3948465200263698 1105 76-77 0.4619351814808823 1105 78-79 0.47017271935548877 1105 80-81 0.48034521017106613 1105 82-83 0.5258326053125515 1105 84-85 0.42225024982280956 1105 86-87 0.4762391540556301 1105 88-89 0.4276522387637023 1105 90-91 0.49137863549738015 1105 92-93 0.46357469049002376 1105 94-95 0.47465846092279307 1105 96-97 0.49634771270098454 1105 98-99 0.4617492564621877 1105 100-101 0.5719101924742134 1106 1 0.2733407778663519 1106 2 0.25070692639881287 1106 3 0.24034775018392907 1106 4 0.19176953426801902 1106 5 0.2139405209995715 1106 6 0.25621808451143124 1106 7 0.3028227784869131 1106 8 0.2526558133311312 1106 9 0.31363840420926437 1106 10-11 0.30138522805376056 1106 12-13 0.2787280495277855 1106 14-15 0.3415672560008929 1106 16-17 0.31817576376227663 1106 18-19 0.31617003084817696 1106 20-21 0.3073138021547308 1106 22-23 0.3183059906124228 1106 24-25 0.3580116032537859 1106 26-27 0.2767726782925166 1106 28-29 0.3382323444691977 1106 30-31 0.32971758448245936 1106 32-33 0.37831177050532716 1106 34-35 0.3580564319394455 1106 36-37 0.3970441443956858 1106 38-39 0.42420649074000494 1106 40-41 0.4377810883857478 1106 42-43 0.46015170755570267 1106 44-45 0.4310185783690912 1106 46-47 0.4685927259922167 1106 48-49 0.5036721387772829 1106 50-51 0.5438322907869804 1106 52-53 0.4941212873333285 1106 54-55 0.4697510322788858 1106 56-57 0.43927966857189205 1106 58-59 0.5568538784016326 1106 60-61 0.5167145036163632 1106 62-63 0.5341412918367752 1106 64-65 0.5383429485267399 1106 66-67 0.5730093801088145 1106 68-69 0.5554900439187804 1106 70-71 0.5405466627205939 1106 72-73 0.5101723365488127 1106 74-75 0.4971205804520338 1106 76-77 0.47127344747018896 1106 78-79 0.46703044456346277 1106 80-81 0.5181395792263714 1106 82-83 0.5054669463485411 1106 84-85 0.4614936727942833 1106 86-87 0.4779549829833165 1106 88-89 0.4728874879671636 1106 90-91 0.52304013259198 1106 92-93 0.46770369472225326 1106 94-95 0.5323577124048207 1106 96-97 0.5176807111364283 1106 98-99 0.5749191554420783 1106 100-101 0.592428791853667 1107 1 0.22070246902482182 1107 2 0.21892116046569754 1107 3 0.17033210935329635 1107 4 0.15403877329573845 1107 5 0.16087118622879615 1107 6 0.15784567478139877 1107 7 0.14599163540987092 1107 8 0.17910634015358085 1107 9 0.23812275646684355 1107 10-11 0.17143053409940734 1107 12-13 0.21145830766288753 1107 14-15 0.19580228380112175 1107 16-17 0.19158172138666174 1107 18-19 0.15638250124188602 1107 20-21 0.16044224653494865 1107 22-23 0.13366008918696792 1107 24-25 0.19953701538519653 1107 26-27 0.1539178215012953 1107 28-29 0.11546107668457495 1107 30-31 0.1811347931431655 1107 32-33 0.24352449241664686 1107 34-35 0.16150494931441983 1107 36-37 0.1890827633603962 1107 38-39 0.23148420036596917 1107 40-41 0.21943856053234612 1107 42-43 0.29377439671783634 1107 44-45 0.20406826129649147 1107 46-47 0.24481183779757743 1107 48-49 0.19479982729755818 1107 50-51 0.15935767681600765 1107 52-53 0.24915792255686142 1107 54-55 0.29438490284459107 1107 56-57 0.3491461044021662 1107 58-59 0.34408255971934665 1107 60-61 0.39918552235101146 1107 62-63 0.39892910246943813 1107 64-65 0.47762521074031383 1107 66-67 0.4149644169985294 1107 68-69 0.4322976930723854 1107 70-71 0.4875295327076543 1107 72-73 0.5177202315424623 1107 74-75 0.472997080710023 1107 76-77 0.4261803005963003 1107 78-79 0.5152039692175947 1107 80-81 0.49460051630335755 1107 82-83 0.5255903406908047 1107 84-85 0.5553707547907294 1107 86-87 0.5180705319565426 1107 88-89 0.4683297412861158 1107 90-91 0.5767220216722357 1107 92-93 0.5504347489997166 1107 94-95 0.6407242932637125 1107 96-97 0.6704215738085644 1107 98-99 0.7419277012183407 1107 100-101 0.8050074619085485 1108 1 0.14370443722246762 1108 2 0.1578831175554427 1108 3 0.13654594655196917 1108 4 0.12145876686285817 1108 5 0.09708237174989875 1108 6 0.0974905448096024 1108 7 0.1604713583861539 1108 8 0.1592813694635069 1108 9 0.16416030432540651 1108 10-11 0.10954976466971544 1108 12-13 0.10993562308546956 1108 14-15 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-1.0469111502833073 2316 54-55 -1.6651851105255844 2316 56-57 -1.2609867311820864 2316 58-59 -0.8706709250311988 2316 60-61 -0.7876106689542652 2316 62-63 -1.267091150506154 2316 64-65 -1.5588264049184133 2316 66-67 -1.1947206650524294 2316 68-69 -1.099719069524788 2316 70-71 -0.9533809703185341 2316 72-73 -1.1112784571037295 2316 74-75 -0.9279664811261767 2316 76-77 -0.86769295728487 2316 78-79 -0.8534233284608383 2316 80-81 -0.7795914398500443 2316 82-83 -0.8853481248650326 2316 84-85 -1.0223998174719462 2316 86-87 -1.104898887189126 2316 88-89 -0.8040184004236508 2316 90-91 -0.7925212967245088 2316 92-93 -0.8039047585630676 2316 94-95 -1.031759782668093 2316 96-97 -1.0771594501888337 2316 98-99 -1.058358407083393 2316 100-101 -1.314101132700685 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 33.0 4 75.0 5 155.0 6 374.0 7 1175.0 8 3058.0 9 6422.0 10 10908.0 11 16461.0 12 23478.0 13 33412.0 14 42422.0 15 52908.0 16 65591.0 17 85989.0 18 72678.0 19 42782.0 20 34103.0 21 33289.0 22 33970.0 23 37284.0 24 44878.0 25 55927.0 26 70455.0 27 89746.0 28 114664.0 29 145478.0 30 185438.0 31 235292.0 32 302580.0 33 399510.0 34 555477.0 35 824156.0 36 1296903.0 37 2053945.0 38 2264373.0 39 548836.0 40 254.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.86336397059763 22.608419564015897 16.802755973346244 22.725460492040224 2 14.21467314372968 21.89182228209156 39.8149989728411 24.078505601337657 3 16.19245045792507 26.930711870587455 32.15943116335177 24.717406508135706 4 11.970511634305346 19.118809615524356 39.042006265235244 29.868672484935054 5 12.237884383771766 37.94451112770016 35.881508124500726 13.936096364027344 6 28.726883234816615 39.634577657358044 17.91266514347738 13.725873964347969 7 25.248610478448374 33.88041044171675 23.119433086040416 17.75154599379446 8 24.762258772384808 35.90771224192428 20.752195082765233 18.577833902925683 9 24.45054799051068 16.975911637986542 22.443859027299983 36.129681344202794 10-11 23.045208018698183 27.728251503580005 29.129490874375453 20.09704960334636 12-13 24.139001519037848 25.319677084624708 29.3663190993331 21.17500229700435 14-15 21.394769891411084 27.154240249604882 27.523991192532133 23.926998666451905 16-17 19.999416422921886 30.42348788382585 28.217975339116023 21.359120354136238 18-19 19.91711874315242 28.554859328242276 31.47240834464376 20.05561358396154 20-21 21.080328065635204 27.71113883879662 31.3108424295319 19.897690666036276 22-23 20.687189093179477 27.64795033115189 31.404081339811206 20.260779235857427 24-25 20.486755151157812 27.778894348074463 31.294486191909154 20.43986430885857 26-27 20.290294796108636 27.804982576833797 31.316425405923027 20.588297221134543 28-29 20.370409929411757 27.771714455616447 31.472364163469706 20.38551145150209 30-31 20.679521314242617 28.07454805739724 31.069706766085066 20.176223862275076 32-33 20.261661699895477 27.91378387730521 31.41135224363772 20.413202179161598 34-35 20.51866288889994 27.763376040100024 31.24279895371241 20.475162117287628 36-37 20.878038494966074 28.015204528877252 30.681209643217077 20.425547332939598 38-39 20.647059602091954 28.151276457648166 30.83033842517545 20.37132551508443 40-41 20.525410258622063 28.002739281928346 30.863598436518195 20.608252022931396 42-43 20.512165359418773 28.05495341825901 30.666866850098874 20.766014372223342 44-45 20.75201283266258 28.211207474750005 30.386362178803367 20.65041751378405 46-47 20.8754653183205 28.46141487430388 29.88235084901718 20.780768958358436 48-49 20.85495856775256 28.3814344366636 29.770595445534255 20.993011550049584 50-51 20.914526635699158 28.48011064210204 29.467913550228552 21.137449171970253 52-53 20.893040107723873 28.578433251313974 29.418096895273283 21.110429745688876 54-55 21.032526813461157 28.92833692638116 29.250599768233208 20.788536491924468 56-57 20.967740039786534 28.591201165643522 29.24600436825043 21.195054426319512 58-59 20.823864614844982 28.309624360628348 29.523432733968352 21.343078290558317 60-61 21.058803099702715 28.626169343818308 29.262944676696605 21.05208287978237 62-63 20.86508445141166 28.644455665442642 29.301068029280366 21.189391853865335 64-65 20.883841794501855 28.518314735569987 29.43051398630398 21.167329483624172 66-67 20.846708778184254 28.566076507501958 29.240329243553916 21.34688547075987 68-69 21.142713610726332 28.751583297751456 29.060646085137403 21.045057006384805 70-71 21.182916597550534 28.45539523338359 29.104289676773615 21.25739849229226 72-73 20.959341329164534 28.637150322062638 29.18806630778363 21.215442040989196 74-75 21.008660164495343 28.76401083689542 29.015887146249288 21.211441852359947 76-77 21.066489702972756 28.822692507167986 28.88960579671248 21.22121199314678 78-79 21.0638531312519 28.832355309773384 29.01111155570212 21.092680003272598 80-81 20.99793814517281 28.757938406813956 28.994928045620405 21.249195402392832 82-83 20.88612255512447 28.787914481422373 29.123206002818446 21.202756960634712 84-85 20.816890983734478 28.821427574948345 28.974722005012243 21.386959436304934 86-87 20.92854007276154 28.88311189220376 28.910687483614854 21.277660551419842 88-89 20.75114712596335 28.801873626297812 28.98592991685093 21.461049330887903 90-91 20.62846646527409 28.73374352826408 29.017553246260807 21.620236760201024 92-93 20.73651371177299 28.859386936958803 28.86656667833481 21.537532672933395 94-95 20.590550497065234 28.67604960125424 29.04001050648837 21.69338939519216 96-97 20.58073209967643 28.800199500023503 28.991794130692956 21.62727426960711 98-99 20.4792268366865 28.75897076037258 28.936988338714166 21.824814064226754 100-101 20.41353954218218 28.843797190479638 28.83862573606992 21.904037531268266 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3333.0 1 2254.0 2 1660.5 3 2239.0 4 1840.5 5 1490.5 6 2627.5 7 5876.5 8 10125.5 9 12419.5 10 11001.0 11 9069.5 12 9277.5 13 10261.0 14 11138.5 15 11926.5 16 13015.0 17 14183.5 18 15487.0 19 17226.0 20 19983.0 21 24501.0 22 31295.0 23 42120.0 24 56114.5 25 71757.0 26 92669.0 27 118817.0 28 150128.0 29 181127.5 30 206496.0 31 231580.5 32 256624.0 33 277885.5 34 296656.5 35 315113.5 36 328149.0 37 339819.5 38 352343.5 39 357196.0 40 358924.0 41 355563.5 42 347731.0 43 341418.5 44 331957.0 45 320937.5 46 312318.0 47 301770.5 48 291036.0 49 284153.0 50 275455.5 51 261553.0 52 242486.5 53 225992.5 54 213927.5 55 201251.0 56 183332.5 57 162507.5 58 146044.5 59 132082.0 60 116173.0 61 98235.5 62 81787.5 63 66320.5 64 52497.0 65 41433.5 66 31919.5 67 23945.5 68 18029.0 69 13785.5 70 10090.5 71 7242.0 72 5260.5 73 3705.5 74 2637.0 75 1888.0 76 1283.0 77 902.5 78 576.5 79 359.5 80 250.5 81 173.0 82 116.0 83 78.5 84 50.0 85 40.0 86 33.0 87 20.5 88 15.5 89 11.5 90 7.5 91 3.5 92 1.0 93 0.5 94 1.5 95 1.5 96 1.5 97 1.0 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05108087979712367 2 0.0021769162458558106 3 2.248458094311166E-4 4 1.4308369691071058E-4 5 0.0025652862803277393 6 0.001921409644229542 7 0.0 8 0.0 9 7.154184845535529E-5 10-11 2.044052813010151E-5 12-13 0.0 14-15 6.029955798379945E-4 16-17 0.0 18-19 3.066079219515226E-5 20-21 3.934801665044541E-4 22-23 0.0 24-25 5.774449196753676E-4 26-27 0.006847576923584005 28-29 0.007164405109600578 30-31 0.001124229047155583 32-33 0.006423435964884399 34-35 0.0076498676526904905 36-37 0.0043385020956140455 38-39 0.002427312715449554 40-41 0.001706784098863476 42-43 0.001262202612033768 44-45 7.71629936911332E-4 46-47 5.314537313826392E-4 48-49 0.0020031717567499476 50-51 0.0014563876292697324 52-53 0.002125814925530557 54-55 8.022907291064843E-4 56-57 1.6863435707333745E-4 58-59 7.358590126836543E-4 60-61 0.005897092365534285 62-63 0.0025295153561000617 64-65 0.001098678386992956 66-67 0.005524052727159933 68-69 0.007261497618218562 70-71 0.0023097796787014705 72-73 5.212334673175885E-4 74-75 0.0032244933125235133 76-77 0.0018243171356115597 78-79 0.002141145321628133 80-81 9.096035017895172E-4 82-83 1.2775330081313442E-4 84-85 4.496916188622332E-4 86-87 0.0047830835824437526 88-89 0.0025346254881325873 90-91 0.0011293391791881084 92-93 8.176211252040605E-5 94-95 5.4167399544769E-4 96-97 2.3506607349616735E-4 98-99 5.723347876428423E-4 100-101 1.533039609757613E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9784483.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.76606480119645 #Duplication Level Percentage of deduplicated Percentage of total 1 68.2688096888012 34.6573881656223 2 17.891423247556485 18.165543039421703 3 6.266681719901065 9.54404310842912 4 2.7492321100766866 5.582707818147325 5 1.4153235690462824 3.59252040104321 6 0.8185090647980965 2.4931490534344123 7 0.5317799287012089 1.889746202429484 8 0.3564636416579029 1.4477005065340456 9 0.26003892121439376 1.188103745268282 >10 1.3084184213833223 11.836867863217668 >50 0.0853168333622216 2.9702919931653273 >100 0.044506490058011765 4.090623518191896 >500 0.0025069268975150805 0.8323340045251957 >1k 9.086663018283499E-4 0.7475012349789445 >5k 2.0192560926677308E-5 0.08241778726306695 >10k+ 6.057768278003193E-5 0.8790615583279904 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 35504 0.362860255365562 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31017 0.31700193050567926 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19233 0.19656633876312118 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.0220264065050754E-5 0.0 3 0.0 0.0 0.0 2.0440528130101508E-5 0.0 4 0.0 0.0 0.0 5.110132032525377E-5 0.0 5 1.0220264065050754E-5 2.0440528130101508E-5 0.0 8.176211252040603E-5 0.0 6 1.0220264065050754E-5 2.0440528130101508E-5 0.0 8.176211252040603E-5 0.0 7 1.0220264065050754E-5 2.0440528130101508E-5 0.0 9.198237658545679E-5 0.0 8 1.0220264065050754E-5 2.0440528130101508E-5 0.0 1.0220264065050755E-4 0.0 9 1.0220264065050754E-5 2.0440528130101508E-5 0.0 1.124229047155583E-4 0.0 10-11 1.0220264065050754E-5 2.0440528130101508E-5 0.0 1.124229047155583E-4 0.0 12-13 1.0220264065050754E-5 4.0881056260203016E-5 0.0 1.2264316878060906E-4 0.0 14-15 1.0220264065050754E-5 4.0881056260203016E-5 0.0 1.5841409300828668E-4 0.0 16-17 1.0220264065050754E-5 4.0881056260203016E-5 0.0 2.1973567739859122E-4 0.0 18-19 1.0220264065050754E-5 5.110132032525377E-5 0.0 2.248458094311166E-4 0.0 20-21 1.0220264065050754E-5 5.110132032525377E-5 0.0 2.4017620552869274E-4 0.0 22-23 1.0220264065050754E-5 5.110132032525377E-5 0.0 2.8616739382142115E-4 0.0 24-25 1.0220264065050754E-5 5.110132032525377E-5 0.0 3.4748897821172567E-4 0.0 26-27 1.0220264065050754E-5 5.110132032525377E-5 0.0 5.110132032525377E-4 0.0 28-29 1.533039609757613E-5 5.110132032525377E-5 0.0 0.00108334799089538 0.0 30-31 2.0440528130101508E-5 5.110132032525377E-5 0.0 0.002503964695937435 0.0 32-33 2.0440528130101508E-5 5.110132032525377E-5 0.0 0.005340087973989019 0.0 34-35 2.0440528130101508E-5 8.176211252040603E-5 0.0 0.010000528387652162 0.0 36-37 2.0440528130101508E-5 8.176211252040603E-5 0.0 0.017839470925546094 0.0 38-39 2.0440528130101508E-5 8.176211252040603E-5 0.0 0.029321937602630615 0.0 40-41 2.0440528130101508E-5 8.176211252040603E-5 0.0 0.042327223625407695 0.0 42-43 2.0440528130101508E-5 8.176211252040603E-5 0.0 0.05654872107192582 0.0 44-45 2.5550660162626887E-5 1.0220264065050755E-4 0.0 0.07325885281828382 0.0 46-47 3.0660792195152265E-5 1.0220264065050755E-4 0.0 0.09477761880724817 0.0 48-49 5.110132032525377E-5 1.0220264065050755E-4 0.0 0.11733885173084771 0.0 50-51 6.132158439030453E-5 1.0220264065050755E-4 0.0 0.14174995245022143 0.0 52-53 7.154184845535527E-5 1.0731277268303293E-4 0.0 0.1663143571305709 0.0 54-55 7.154184845535527E-5 1.124229047155583E-4 0.0 0.19491576611661546 0.0 56-57 7.154184845535527E-5 1.124229047155583E-4 0.0 0.22869884898364073 0.0 58-59 7.154184845535527E-5 1.4308369691071055E-4 0.0 0.2682461607833546 0.0 60-61 7.154184845535527E-5 1.4308369691071055E-4 0.0 0.31094131391510416 0.0 62-63 7.154184845535527E-5 1.4308369691071055E-4 0.0 0.35508263441205834 0.0 64-65 7.154184845535527E-5 1.4819382894323593E-4 0.0 0.40530501202771774 0.0 66-67 7.154184845535527E-5 1.5330396097576132E-4 0.0 0.4661973453272902 0.0 68-69 7.154184845535527E-5 1.5330396097576132E-4 0.0 0.5344738194138617 0.0 70-71 7.154184845535527E-5 1.5330396097576132E-4 0.0 0.6094241259349115 0.0 72-73 7.154184845535527E-5 1.7374448910586283E-4 0.0 0.6905423618192192 0.0 74-75 1.0220264065050755E-4 1.7374448910586283E-4 0.0 0.7764539015500359 0.0 76-77 1.0731277268303293E-4 1.7374448910586283E-4 0.0 0.8711088771885035 0.0 78-79 1.124229047155583E-4 1.7374448910586283E-4 0.0 0.9830769801531669 0.0 80-81 1.124229047155583E-4 1.7374448910586283E-4 0.0 1.1111062280960577 0.0 82-83 1.124229047155583E-4 1.7374448910586283E-4 0.0 1.2471583833300135 0.0 84-85 1.124229047155583E-4 1.9418501723596432E-4 0.0 1.3868131816468994 0.0 86-87 1.124229047155583E-4 2.1973567739859122E-4 0.0 1.54193634962624 0.0 88-89 1.124229047155583E-4 2.3506607349616735E-4 0.0 1.7171781074176327 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 18445 0.0 60.110252 1 GTATCAA 34660 0.0 42.46441 1 ATCAACG 43850 0.0 33.27548 3 TCAACGC 44205 0.0 33.00842 4 CAACGCA 45620 0.0 32.067886 5 TATCAAC 46030 0.0 31.834822 2 GTGGTAT 7105 0.0 31.236708 1 AACGCAG 48090 0.0 30.62776 6 TATAACG 955 0.0 29.842562 2 TGGTATC 7740 0.0 27.861412 2 ACGCAGA 54975 0.0 26.51537 7 CGCAGAG 54895 0.0 26.415573 8 GCAGAGT 58865 0.0 24.085363 9 ATAACGC 1375 0.0 21.762589 3 GAGTACT 41020 0.0 21.606382 12-13 AGAGTAC 55055 0.0 21.55066 10-11 GTACATG 28295 0.0 21.129215 1 CAGAGTA 57905 0.0 21.051844 10-11 AGTACTT 43260 0.0 19.96059 12-13 GTACTTT 44705 0.0 19.644865 14-15 >>END_MODULE