FastQCFastQC Report
Wed 25 May 2016
SRR2049408_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049408_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10509883
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT454780.4327165202505109No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT385290.366597801326618No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212350.20204792003869118No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA209900.060.267221
GTATCAA408050.043.2947461
ATCAACG500750.034.917283
TCAACGC503750.034.6812134
CAACGCA521000.033.5316625
GTGGTAT79600.033.478981
TATCAAC527900.033.056922
AACGCAG544700.032.238536
TGGTATC86300.030.0508482
ACGCAGA612000.028.4239987
CGCAGAG616850.028.0155628
GCAGAGT647800.025.9364369
GTACATG374000.023.3705331
CAGAGTA628550.022.31337410-11
TACATGG366100.022.2893962
GAGTACT442750.021.97864212-13
AGAGTAC607850.021.68220710-11
GTACTTT456300.021.09473814-15
AGTACTT446750.020.80361212-13
ACATGGG381900.020.3856623
Produced by FastQC (version 0.11.5)