##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049408_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10509883 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.406229165443612 31.0 31.0 34.0 30.0 34.0 2 31.442687801567345 31.0 31.0 34.0 28.0 34.0 3 31.461879071346466 33.0 31.0 34.0 28.0 34.0 4 35.08012753329414 37.0 35.0 37.0 32.0 37.0 5 35.10480525806044 37.0 35.0 37.0 32.0 37.0 6 35.193400250031324 37.0 35.0 37.0 32.0 37.0 7 35.1415181310772 37.0 35.0 37.0 32.0 37.0 8 35.11579263061254 37.0 35.0 37.0 32.0 37.0 9 36.69107229833101 39.0 37.0 39.0 33.0 39.0 10-11 36.621806541519064 39.0 37.0 39.0 32.5 39.0 12-13 36.579792420143974 39.0 37.0 39.0 32.0 39.0 14-15 37.73604986849045 40.0 38.0 41.0 32.0 41.0 16-17 37.72320938301597 40.0 38.0 41.0 32.5 41.0 18-19 37.76055770554249 40.0 38.0 41.0 33.0 41.0 20-21 37.7248162515225 40.0 38.0 41.0 32.0 41.0 22-23 37.62503374205022 40.0 38.0 41.0 32.0 41.0 24-25 37.5189257102101 40.0 37.5 41.0 32.0 41.0 26-27 37.37803613037367 40.0 37.0 41.0 32.0 41.0 28-29 37.226734541193274 40.0 37.0 41.0 31.5 41.0 30-31 37.0349161831773 40.0 37.0 41.0 31.0 41.0 32-33 36.78921183042665 40.0 36.0 41.0 30.5 41.0 34-35 36.5946681328422 39.0 36.0 41.0 30.0 41.0 36-37 36.41050604464388 39.0 35.5 41.0 30.0 41.0 38-39 36.217618883102695 39.0 35.0 41.0 30.0 41.0 40-41 36.01219509294252 39.0 35.0 40.0 29.5 41.0 42-43 35.78199200695194 39.0 35.0 40.0 28.5 41.0 44-45 35.50337848670627 39.0 35.0 40.0 27.5 41.0 46-47 35.14115799386159 38.0 35.0 40.0 26.0 41.0 48-49 34.844932621990175 38.0 34.0 40.0 24.5 41.0 50-51 33.48190436563375 37.0 32.5 39.0 23.0 40.0 52-53 33.77460686289277 37.5 33.5 39.0 23.5 40.0 54-55 34.81683292763583 38.5 34.5 40.0 25.5 41.0 56-57 34.93388023444219 39.0 35.0 40.0 26.0 41.0 58-59 34.77955563349278 38.5 34.5 40.0 25.5 41.0 60-61 34.52396249320758 38.0 34.0 40.0 24.5 41.0 62-63 34.256770413143514 38.0 34.0 40.0 23.5 41.0 64-65 33.97845304272179 37.5 34.0 40.0 23.0 41.0 66-67 33.654841305083984 37.0 33.5 40.0 22.5 41.0 68-69 33.31089128204377 36.0 33.0 39.5 22.0 41.0 70-71 32.977421775294744 36.0 33.0 39.0 20.5 41.0 72-73 32.717071493564674 35.5 33.0 39.0 20.0 40.0 74-75 32.51155921526434 35.0 33.0 38.5 20.0 40.0 76-77 32.1730306131857 35.0 33.0 37.5 18.5 40.0 78-79 31.799130447027814 35.0 32.0 37.0 18.0 39.0 80-81 31.45592557976145 35.0 32.0 36.5 15.0 39.0 82-83 31.115297667918853 35.0 32.0 36.0 11.0 38.5 84-85 30.795128642250347 35.0 32.0 36.0 7.5 37.0 86-87 30.501029126584946 35.0 31.0 35.0 4.5 37.0 88-89 30.213746480336653 35.0 31.0 35.0 2.0 36.5 90-91 29.944223784413204 34.5 31.0 35.0 2.0 36.0 92-93 29.698240313426894 34.0 31.0 35.0 2.0 36.0 94-95 29.44031998262968 34.0 31.0 35.0 2.0 36.0 96-97 29.184902629268088 34.0 30.5 35.0 2.0 35.0 98-99 28.871144521780117 34.0 30.0 35.0 2.0 35.0 100-101 28.33941957298668 34.0 29.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.33415501181595175 1101 2 0.3540521257684581 1101 3 0.3757653966899923 1101 4 0.4067483896554194 1101 5 0.33772586033305174 1101 6 0.3247384382224965 1101 7 0.3592643318726161 1101 8 0.31354162259083296 1101 9 0.3926212893596599 1101 10-11 0.39252728211805277 1101 12-13 0.3801757462576063 1101 14-15 0.4743672319834644 1101 16-17 0.46005647297944563 1101 18-19 0.4235053752364806 1101 20-21 0.4288978377552084 1101 22-23 0.39787079987461027 1101 24-25 0.42802015117397474 1101 26-27 0.4349786130104576 1101 28-29 0.4249112833105073 1101 30-31 0.34962414948635967 1101 32-33 0.26812725021510886 1101 34-35 0.35301108877437315 1101 36-37 0.3810351208829772 1101 38-39 0.28692224661621424 1101 40-41 0.1965843649788681 1101 42-43 0.16309736261452912 1101 44-45 0.1470814534918361 1101 46-47 0.1404773987017478 1101 48-49 0.05526144937380906 1101 50-51 0.04552033824828072 1101 52-53 0.1329242490712801 1101 54-55 0.35737760693964304 1101 56-57 0.33747423628538087 1101 58-59 0.32688754412096444 1101 60-61 0.40794251422583017 1101 62-63 0.3169295043213012 1101 64-65 0.32959291707957306 1101 66-67 0.32092415547039366 1101 68-69 0.27100187798991726 1101 70-71 0.20758642093641555 1101 72-73 0.1979379521052138 1101 74-75 0.2023801703170065 1101 76-77 0.18399407760492892 1101 78-79 0.06969412695309529 1101 80-81 -0.01855667223666657 1101 82-83 -0.010917017655888372 1101 84-85 -0.03462732607628283 1101 86-87 -0.09246835408592702 1101 88-89 -0.1459441554255072 1101 90-91 -0.14287920775011642 1101 92-93 -0.17786339938738038 1101 94-95 -0.3063562081512181 1101 96-97 -0.31390228662467123 1101 98-99 -0.4793228903963289 1101 100-101 -0.532933766331201 1102 1 0.29898629937819265 1102 2 0.29490902979529565 1102 3 0.29454355958765177 1102 4 0.31542929799079644 1102 5 0.31789712424770045 1102 6 0.3562905334890303 1102 7 0.32296007735468635 1102 8 0.3593564186111209 1102 9 0.39905979676125725 1102 10-11 0.42665037529001637 1102 12-13 0.3787930541252962 1102 14-15 0.44067998414381293 1102 16-17 0.35549844401222686 1102 18-19 0.3481146628910352 1102 20-21 0.32406763022702734 1102 22-23 0.33290633729741614 1102 24-25 0.3209961715465468 1102 26-27 0.3420823440018381 1102 28-29 0.35111155941398664 1102 30-31 0.3306526057005428 1102 32-33 0.32197723316493665 1102 34-35 0.2508831740863755 1102 36-37 0.2441484209082958 1102 38-39 0.1782234375072136 1102 40-41 0.22178538080964216 1102 42-43 0.1325289955410014 1102 44-45 0.17857860089613808 1102 46-47 0.1717977649044684 1102 48-49 0.15064206502015054 1102 50-51 0.07206744725404235 1102 52-53 0.24056692289253334 1102 54-55 0.3622873135225788 1102 56-57 0.4211617133686687 1102 58-59 0.4308839968040985 1102 60-61 0.46149913593843195 1102 62-63 0.4103781704542868 1102 64-65 0.32711610206008146 1102 66-67 0.3479047608850294 1102 68-69 0.34760241770756295 1102 70-71 0.3045754418988196 1102 72-73 0.25680994748027075 1102 74-75 0.1903049857120962 1102 76-77 0.2300596576831495 1102 78-79 0.20079738672878022 1102 80-81 0.16617908089074618 1102 82-83 0.14474971588213492 1102 84-85 0.1136605963534727 1102 86-87 0.03827852834427503 1102 88-89 0.030422764792145784 1102 90-91 -0.07180701702935721 1102 92-93 -0.17000522431523635 1102 94-95 -0.2218335358008936 1102 96-97 -0.2741765591637879 1102 98-99 -0.3245631378615812 1102 100-101 -0.34636161638944785 1103 1 0.2935904352989418 1103 2 0.24313624649505883 1103 3 0.2842442817902793 1103 4 0.29984487126728965 1103 5 0.2837522615990906 1103 6 0.31549366884227936 1103 7 0.31994340835917257 1103 8 0.30822246635864303 1103 9 0.40583081958470046 1103 10-11 0.3813822090206642 1103 12-13 0.3778907079846192 1103 14-15 0.378270308328851 1103 16-17 0.45667407119633197 1103 18-19 0.47156447431970605 1103 20-21 0.4535064284429424 1103 22-23 0.430282947308541 1103 24-25 0.43313615458560406 1103 26-27 0.4516303601784628 1103 28-29 0.44866684994802597 1103 30-31 0.4763680107030339 1103 32-33 0.4084983839120184 1103 34-35 0.3999934547388335 1103 36-37 0.3974871165573006 1103 38-39 0.36707277673942684 1103 40-41 0.33670249834401744 1103 42-43 0.30979296706590276 1103 44-45 0.3060060421639079 1103 46-47 0.3259499414302951 1103 48-49 0.2646951212198019 1103 50-51 0.19701124825317606 1103 52-53 0.29687019251538516 1103 54-55 0.4395862502988237 1103 56-57 0.5022219660804197 1103 58-59 0.468719455032371 1103 60-61 0.44412555614052707 1103 62-63 0.4469911884500206 1103 64-65 0.4334100047304119 1103 66-67 0.4312853496931268 1103 68-69 0.4278909486165574 1103 70-71 0.3678247234581775 1103 72-73 0.40762059737142664 1103 74-75 0.3368000246201035 1103 76-77 0.29884594546350485 1103 78-79 0.2965583942144221 1103 80-81 0.27000614463586103 1103 82-83 0.29468284164966363 1103 84-85 0.22244199355861838 1103 86-87 0.21374825753484927 1103 88-89 0.2331134931539971 1103 90-91 0.14403947536585093 1103 92-93 0.14722334278911475 1103 94-95 0.12930839369687064 1103 96-97 0.1448955224537194 1103 98-99 0.20423312975390928 1103 100-101 0.2622395803004878 1104 1 0.08416205488618189 1104 2 0.13777275254403776 1104 3 0.1776997847813817 1104 4 0.11011988690987096 1104 5 0.11212343041265882 1104 6 0.038918010769251055 1104 7 -0.00987958517133336 1104 8 0.07196404696856007 1104 9 0.040699263316639644 1104 10-11 0.09969567714909289 1104 12-13 0.09770963216314499 1104 14-15 0.1337917369434649 1104 16-17 0.13338407675330188 1104 18-19 0.08409444024704271 1104 20-21 0.09143290559968165 1104 22-23 0.10380778202326724 1104 24-25 0.09212917850818059 1104 26-27 0.03923630095504649 1104 28-29 0.04242774526273507 1104 30-31 0.09440312359426883 1104 32-33 0.08774529074310777 1104 34-35 0.011325327178518307 1104 36-37 0.07077694128931 1104 38-39 -0.0036230071403835495 1104 40-41 -0.004719739378472809 1104 42-43 -0.06719308937086055 1104 44-45 -0.030036117659108186 1104 46-47 -0.06356114605563334 1104 48-49 -0.08184321421847329 1104 50-51 -0.2435699547030552 1104 52-53 -0.15812553500975923 1104 54-55 -0.01830924186895544 1104 56-57 -0.11195452818000717 1104 58-59 -0.0656562804925187 1104 60-61 -0.14762739266794966 1104 62-63 -0.12802719337452118 1104 64-65 -0.12990315632714555 1104 66-67 -0.025442265503258454 1104 68-69 -0.04395384779439837 1104 70-71 -0.03707308081008165 1104 72-73 -0.17126373513021065 1104 74-75 -0.11231247161575908 1104 76-77 -0.05476757598599846 1104 78-79 -0.08401779272056586 1104 80-81 -0.11831872913361607 1104 82-83 -0.11438811675406058 1104 84-85 -0.12401493362612825 1104 86-87 -0.12375415952127966 1104 88-89 -0.09733477940529056 1104 90-91 -0.14350578576690154 1104 92-93 -0.18498471368986458 1104 94-95 -0.22472609062718973 1104 96-97 -0.24103565697102525 1104 98-99 -0.24036384688674772 1104 100-101 -0.29673746426912473 1105 1 0.32400353994155395 1105 2 0.35066530554306397 1105 3 0.3068580254362949 1105 4 0.3162438966615895 1105 5 0.31509909730412033 1105 6 0.3479559667976204 1105 7 0.3252360797680751 1105 8 0.33724697808342086 1105 9 0.46129272050412595 1105 10-11 0.38661100669573756 1105 12-13 0.32346546866291703 1105 14-15 0.4487582950781217 1105 16-17 0.42476687432270666 1105 18-19 0.4287315256116244 1105 20-21 0.4621063466551547 1105 22-23 0.40966408504660023 1105 24-25 0.40540081050922794 1105 26-27 0.4457929065604418 1105 28-29 0.5054147370822832 1105 30-31 0.49120479467085687 1105 32-33 0.47544039818465933 1105 34-35 0.556979938013491 1105 36-37 0.5190648523967525 1105 38-39 0.4950401746684463 1105 40-41 0.4725979368491906 1105 42-43 0.5052252108978337 1105 44-45 0.4646497460044614 1105 46-47 0.4811067809418361 1105 48-49 0.46095272607136195 1105 50-51 0.3707873118086127 1105 52-53 0.4961350222932026 1105 54-55 0.647596937254967 1105 56-57 0.6424653827709861 1105 58-59 0.6466801233760364 1105 60-61 0.6354247939232991 1105 62-63 0.6794557260995902 1105 64-65 0.6761549358183743 1105 66-67 0.6657818081405225 1105 68-69 0.6384620594422259 1105 70-71 0.6593935840169323 1105 72-73 0.7017119146501685 1105 74-75 0.698183843374558 1105 76-77 0.6379486444849789 1105 78-79 0.6001134479991137 1105 80-81 0.6258863371161745 1105 82-83 0.6588034722285308 1105 84-85 0.6442409908827855 1105 86-87 0.6169818701236593 1105 88-89 0.5626076895014727 1105 90-91 0.5190335151010181 1105 92-93 0.5372860347975994 1105 94-95 0.5218147767816639 1105 96-97 0.5676553914882341 1105 98-99 0.5375717538069047 1105 100-101 0.5125157829987863 1106 1 0.2251706284142827 1106 2 0.24558977068350885 1106 3 0.2537993695049181 1106 4 0.27514043373816577 1106 5 0.2996154817916903 1106 6 0.2969488591519678 1106 7 0.32656827515529585 1106 8 0.27593748229993054 1106 9 0.35213915189301304 1106 10-11 0.37952276084004666 1106 12-13 0.366082299865667 1106 14-15 0.3815566478082957 1106 16-17 0.4091661894055463 1106 18-19 0.4228648007461544 1106 20-21 0.4364852894962965 1106 22-23 0.4366835625219636 1106 24-25 0.4365354294369439 1106 26-27 0.48461118206714104 1106 28-29 0.4569129252174804 1106 30-31 0.4410967181635641 1106 32-33 0.5001994749250755 1106 34-35 0.5013324260357379 1106 36-37 0.4874210961776768 1106 38-39 0.45801304045339464 1106 40-41 0.476655058618654 1106 42-43 0.4822285307878573 1106 44-45 0.5072481680005794 1106 46-47 0.5043260020611058 1106 48-49 0.43081543029597924 1106 50-51 0.3592251772779349 1106 52-53 0.4903437751749067 1106 54-55 0.514073620324794 1106 56-57 0.5560142581670178 1106 58-59 0.590131086250743 1106 60-61 0.5557250398431322 1106 62-63 0.4895913215655838 1106 64-65 0.463198706258126 1106 66-67 0.4646698824171551 1106 68-69 0.48353645310629645 1106 70-71 0.5643935087583358 1106 72-73 0.5560047316836929 1106 74-75 0.5838477631275296 1106 76-77 0.5563454135044097 1106 78-79 0.5635175143842162 1106 80-81 0.5329717444475328 1106 82-83 0.5233020378313604 1106 84-85 0.6030866791007625 1106 86-87 0.5645068956210686 1106 88-89 0.6453544171181598 1106 90-91 0.5743253699729678 1106 92-93 0.61236830400793 1106 94-95 0.652472670017854 1106 96-97 0.7193902790658804 1106 98-99 0.6943689014528722 1106 100-101 0.7366809839930859 1107 1 0.2181644003409886 1107 2 0.2042670112511793 1107 3 0.19053829147963697 1107 4 0.2817075370155493 1107 5 0.1805993127210428 1107 6 0.2105780373962176 1107 7 0.18465499039616873 1107 8 0.2882820321519759 1107 9 0.32256294456821877 1107 10-11 0.28669528745466977 1107 12-13 0.32561296000775997 1107 14-15 0.40865978454910135 1107 16-17 0.3398749120003828 1107 18-19 0.31472175716562845 1107 20-21 0.3864168811594979 1107 22-23 0.4088338480731153 1107 24-25 0.39283866833797276 1107 26-27 0.44977666836508945 1107 28-29 0.4435793974327211 1107 30-31 0.41423295930690784 1107 32-33 0.44384568376028 1107 34-35 0.4250823627900715 1107 36-37 0.4087100176556291 1107 38-39 0.4620077743612967 1107 40-41 0.423870383448282 1107 42-43 0.38345994044571086 1107 44-45 0.39041985847405414 1107 46-47 0.39905707662652645 1107 48-49 0.32690627715299314 1107 50-51 0.36765932077987173 1107 52-53 0.40639112382582 1107 54-55 0.41299324170606155 1107 56-57 0.40839830634902086 1107 58-59 0.4536667196499309 1107 60-61 0.4907438754524662 1107 62-63 0.5331969739962332 1107 64-65 0.5029604736281996 1107 66-67 0.5282903422731593 1107 68-69 0.5209309190407225 1107 70-71 0.48928051387487415 1107 72-73 0.5073887112202513 1107 74-75 0.5071612994492583 1107 76-77 0.4994963032938031 1107 78-79 0.4939354154535138 1107 80-81 0.4430042650871151 1107 82-83 0.4816027756476906 1107 84-85 0.5590497727300523 1107 86-87 0.4803770670074421 1107 88-89 0.5291540733324531 1107 90-91 0.5896334013440452 1107 92-93 0.5551726930968321 1107 94-95 0.5786049603150758 1107 96-97 0.5421830848431206 1107 98-99 0.6223464711227784 1107 100-101 0.6327535691023449 1108 1 0.1307532992403999 1108 2 0.13738682215365472 1108 3 0.15168662590524917 1108 4 0.13427075221409268 1108 5 0.17339139821033456 1108 6 0.18070458990816718 1108 7 0.20219295388121594 1108 8 0.17735486560978586 1108 9 0.1589174865993357 1108 10-11 0.2415904839384595 1108 12-13 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-0.8338203533218014 2316 100-101 -0.8088209273467655 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 102137.0 3 22300.0 4 17395.0 5 15031.0 6 15640.0 7 16860.0 8 19261.0 9 23043.0 10 27052.0 11 31353.0 12 37186.0 13 43198.0 14 48461.0 15 58158.0 16 72496.0 17 91987.0 18 77030.0 19 49127.0 20 44790.0 21 45382.0 22 47730.0 23 52759.0 24 60254.0 25 70747.0 26 84731.0 27 103319.0 28 126046.0 29 155017.0 30 192949.0 31 244419.0 32 316812.0 33 425259.0 34 598844.0 35 899303.0 36 1392351.0 37 2114168.0 38 2220697.0 39 546341.0 40 250.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.28892010842015 20.69605597590266 15.384037823531107 21.630986092146088 2 15.472753441323514 20.70985664934325 40.67346282030861 23.143927089024626 3 17.841340465303485 26.605071513970476 31.229170601604174 24.32441741912187 4 12.492013019717506 17.940382467285374 39.41400043066654 30.153604082330578 5 12.649383388684212 38.11400774852067 35.482275808476025 13.754333054319096 6 28.954064070263897 38.800892286468375 18.225359331045873 14.019684312221855 7 25.91347964434576 33.856926106195786 22.941165724272626 17.288428525185832 8 25.207046360610352 35.583932680982755 20.47412993333068 18.734891025076212 9 25.263500765501806 17.131101545153662 22.027020610264188 35.57837707908035 10-11 23.577800245025156 27.452918297194106 28.829659938386875 20.13962151939386 12-13 24.61239487602079 25.25479064651128 28.991984980521497 21.140829496946434 14-15 22.000646270021928 26.847680949659164 27.18383270664869 23.967840073670217 16-17 20.625988593476087 30.221184215922563 27.80688705729591 21.345940133305433 18-19 20.55441491627898 28.47401019510284 30.88895406827612 20.082620820342058 20-21 21.56761168596595 27.483345786785918 30.907087411628936 20.0419551156192 22-23 21.32677747170838 27.321598200837784 30.952373363078323 20.39925096437552 24-25 20.95128091173627 27.67223336305826 30.79659597119097 20.579889754014495 26-27 20.66883563220972 27.762700275515016 30.812561870990162 20.755902221285105 28-29 20.850761720484805 27.62352086175623 30.99984790376709 20.52586951399188 30-31 21.04535417833531 27.879097516890265 30.683882429827214 20.39166587494721 32-33 20.757862490072306 27.829928857442088 30.808227185003478 20.60398146748213 34-35 21.08811005138201 27.633800326916756 30.710103391064397 20.567986230636834 36-37 21.321435486779176 27.694201904993886 30.320759447541306 20.663603160685632 38-39 21.058787551413264 27.9894147220884 30.403267803021038 20.548529923477293 40-41 20.98089865001966 27.782505467026684 30.49520688113888 20.74138900181477 42-43 20.93244448897515 27.968410600535137 30.301358238939883 20.79778667154982 44-45 21.077670592099743 28.065203461313526 29.992176284989526 20.86494966159721 46-47 21.373107293836707 28.026494424769442 29.623540673344213 20.976857608049638 48-49 21.359352035304223 28.170328140554616 29.393621177661238 21.076698646479922 50-51 21.38298477176681 28.347260333443952 29.083824864018766 21.185930030770475 52-53 21.344390514069453 28.473141261685285 29.025813146714462 21.156655077530793 54-55 21.315150635386246 28.73958577320595 29.01886064056609 20.926402950841712 56-57 21.377489823422664 28.461854921036878 28.992275368936166 21.168379886604292 58-59 21.322956835594486 28.15658874254476 29.18968057642431 21.33077384543645 60-61 21.445831613103163 28.397247238441835 29.141523712724688 21.015397435730314 62-63 21.17950169606029 28.504269885295898 29.15873030976293 21.157498108880883 64-65 21.357961122475537 28.412575857101935 29.083264511633622 21.146198508788903 66-67 21.26454904017535 28.35899139341564 29.074678000020555 21.30178156638846 68-69 21.449287400931038 28.5940893529254 28.87672928381185 21.07989396233171 70-71 21.51865250813561 28.38267159762272 28.877944565090075 21.220731329151594 72-73 21.313566675978652 28.52611079690558 28.885386638999666 21.274935888116104 74-75 21.339726781428713 28.650670201173345 28.780621363038538 21.228981654359405 76-77 21.46214434127841 28.638761991086977 28.620829748510406 21.278263919124207 78-79 21.451894200655037 28.675772565083594 28.706812048553097 21.16552118570827 80-81 21.44170568028144 28.710975322764565 28.625040217400926 21.22227877955307 82-83 21.33801357957854 28.710210301801087 28.781063959188753 21.170712159431627 84-85 21.27924423593733 28.68897346406326 28.640105798899494 21.391676501099916 86-87 21.399295251166684 28.715351086031042 28.604820160779866 21.280533502022404 88-89 21.332193293142033 28.696951569193452 28.66537558954595 21.305479548118566 90-91 21.16355182804772 28.61703715364985 28.72758355956668 21.491827458735756 92-93 21.273630628944087 28.760211602554662 28.589351983232785 21.37680578526846 94-95 21.26472137355353 28.491364908708917 28.764753605493333 21.479160112244223 96-97 21.350978864250763 28.63694896112931 28.634736720263 21.377335454356928 98-99 21.237662768762842 28.600400477848186 28.591727121262515 21.570209632126456 100-101 21.2211909111935 28.5810321929448 28.582787883678495 21.61498901218321 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5268.0 1 3443.5 2 2428.5 3 3327.0 4 2722.5 5 2291.0 6 3841.0 7 8036.0 8 14227.0 9 17628.5 10 14951.5 11 11557.0 12 11284.0 13 12044.0 14 12812.0 15 13541.0 16 14499.0 17 15575.0 18 16820.5 19 18576.0 20 21460.5 21 25458.5 22 31646.0 23 41645.0 24 54647.0 25 69690.5 26 89714.5 27 114423.5 28 141587.0 29 169943.0 30 195096.5 31 218036.0 32 243073.0 33 265422.0 34 283024.0 35 302273.5 36 321288.5 37 339290.0 38 360257.5 39 372543.0 40 381155.0 41 387851.5 42 388861.5 43 391661.0 44 383955.0 45 372144.0 46 366100.0 47 357735.0 48 347573.0 49 336731.5 50 322950.5 51 303149.0 52 274701.0 53 248982.5 54 230986.0 55 215322.0 56 194915.5 57 170885.5 58 152004.0 59 137720.5 60 122637.0 61 105676.0 62 89476.5 63 74414.5 64 61695.5 65 51161.5 66 41319.5 67 32448.0 68 25234.5 69 19443.0 70 14710.0 71 10972.5 72 8281.5 73 6176.5 74 4604.0 75 3377.0 76 2383.5 77 1688.0 78 1279.5 79 920.5 80 671.0 81 542.0 82 432.5 83 373.0 84 301.0 85 231.0 86 216.0 87 200.0 88 157.0 89 135.0 90 129.0 91 103.0 92 83.0 93 69.5 94 55.5 95 42.0 96 32.0 97 27.5 98 21.5 99 13.5 100 5.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014310340086564236 2 0.0038915751964127476 3 0.003786911804822185 4 0.005632793438328476 5 0.0025119213981735096 6 0.0066794273542341055 7 0.001189356722620033 8 0.003815456366165066 9 0.010371190621246688 10-11 0.00721225916596788 12-13 0.008211318812968707 14-15 0.003273109700650331 16-17 0.006032417297128807 18-19 0.004048570283798592 20-21 7.61188302476821E-4 22-23 9.752725125484271E-4 24-25 0.007112353201267798 26-27 0.0010561487696865892 28-29 8.515794133959435E-4 30-31 5.471040924052152E-4 32-33 0.001332079529334437 34-35 0.0016555845578870858 36-37 0.0013130498217725165 38-39 0.0018268519259443707 40-41 0.002716490754464155 42-43 0.0026784313393403143 44-45 0.0076975167087968525 46-47 0.014115285584054551 48-49 0.005185595310623344 50-51 0.005903966771085844 52-53 0.014062953888259271 54-55 0.008387343607916473 56-57 0.0055043429122855125 58-59 0.0071313829088297176 60-61 0.0013891686520201986 62-63 0.0022169609309637415 64-65 0.0022597777729780623 66-67 0.0024262877141448674 68-69 0.0021075401124826987 70-71 0.002131327246935099 72-73 0.0012464458453057947 74-75 0.0015747083007489236 76-77 0.008368313900354552 78-79 0.006541461974410181 80-81 0.01305437938747748 82-83 0.015261825464660265 84-85 0.008458705011273675 86-87 0.00577551624504288 88-89 0.0029686343796596023 90-91 0.0025642530939687914 92-93 8.135199982721025E-4 94-95 0.001156054734386672 96-97 0.0016888865461204467 98-99 0.006541461974410181 100-101 0.011403552256480876 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.0509883E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.52239429346036 #Duplication Level Percentage of deduplicated Percentage of total 1 67.45867607284832 30.034217746301735 2 17.052143955460004 15.184045534676741 3 6.393276578092283 8.539319419109088 4 2.9889708365939938 5.323045524739674 5 1.6272341952660136 3.6224181224717564 6 0.9648392402461583 2.5774171850425316 7 0.6728391935837865 2.0969488310981874 8 0.4779967936798778 1.7025249375380271 9 0.3475884015110491 1.3927921077517689 >10 1.79674379475656 14.490123391861163 >50 0.1279532732345528 3.9582239239586565 >100 0.08430174595518518 7.0427817218323225 >500 0.005643016207329948 1.692969793750924 >1k 0.0016861824977458823 1.2012218763773619 >5k 4.268802690053955E-5 0.14184927819034923 >10k+ 6.403204035080932E-5 1.0001006052997075 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 45478 0.4327165202505109 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 38529 0.366597801326618 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21235 0.20204792003869118 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 9.514853780960263E-6 0.0 0.0 1.9029707561920527E-5 0.0 9 9.514853780960263E-6 0.0 9.514853780960263E-6 2.854456134288079E-5 0.0 10-11 9.514853780960263E-6 9.514853780960263E-6 9.514853780960263E-6 4.281684201432119E-5 0.0 12-13 9.514853780960263E-6 9.514853780960263E-6 9.514853780960263E-6 4.757426890480132E-5 0.0 14-15 9.514853780960263E-6 9.514853780960263E-6 9.514853780960263E-6 4.757426890480132E-5 0.0 16-17 1.4272280671440396E-5 1.9029707561920527E-5 9.514853780960263E-6 4.757426890480132E-5 0.0 18-19 1.9029707561920527E-5 2.854456134288079E-5 9.514853780960263E-6 5.708912268576158E-5 0.0 20-21 1.9029707561920527E-5 2.854456134288079E-5 9.514853780960263E-6 8.087625713816223E-5 0.0 22-23 1.9029707561920527E-5 2.854456134288079E-5 9.514853780960263E-6 1.2369309915248342E-4 0.0 24-25 1.9029707561920527E-5 2.854456134288079E-5 9.514853780960263E-6 2.569010520859271E-4 0.0 26-27 1.9029707561920527E-5 2.854456134288079E-5 9.514853780960263E-6 4.614704083765728E-4 0.0 28-29 1.9029707561920527E-5 2.854456134288079E-5 9.514853780960263E-6 0.0011417824537152317 0.0 30-31 1.9029707561920527E-5 2.854456134288079E-5 9.514853780960263E-6 0.002564253093968791 0.0 32-33 2.854456134288079E-5 3.805941512384105E-5 9.514853780960263E-6 0.005609006303876075 0.0 34-35 3.805941512384105E-5 3.805941512384105E-5 9.514853780960263E-6 0.010518670854851572 0.0 36-37 3.805941512384105E-5 3.805941512384105E-5 9.514853780960263E-6 0.018592024287996355 0.0 38-39 5.708912268576158E-5 3.805941512384105E-5 9.514853780960263E-6 0.03025247759656316 0.0 40-41 6.660397646672184E-5 3.805941512384105E-5 9.514853780960263E-6 0.04255518353534478 0.0 42-43 8.087625713816223E-5 3.805941512384105E-5 9.514853780960263E-6 0.05548586982366978 0.0 44-45 9.039111091912252E-5 4.281684201432119E-5 9.514853780960263E-6 0.0722891016008456 0.0 46-47 9.514853780960264E-5 4.757426890480132E-5 9.514853780960263E-6 0.09347392354415363 0.0 48-49 9.514853780960264E-5 4.757426890480132E-5 9.514853780960263E-6 0.11677580045372532 0.0 50-51 9.514853780960264E-5 4.757426890480132E-5 9.514853780960263E-6 0.1417047173598412 0.0 52-53 9.514853780960264E-5 4.757426890480132E-5 9.514853780960263E-6 0.1657439954374373 0.0 54-55 9.514853780960264E-5 4.757426890480132E-5 9.514853780960263E-6 0.19372242298035097 0.0 56-57 9.514853780960264E-5 6.660397646672185E-5 9.514853780960263E-6 0.22806153027583656 0.0 58-59 9.514853780960264E-5 7.61188302476821E-5 9.514853780960263E-6 0.26762429229706935 0.0 60-61 9.514853780960264E-5 7.61188302476821E-5 9.514853780960263E-6 0.30983694109629956 0.0 62-63 9.514853780960264E-5 7.61188302476821E-5 9.514853780960263E-6 0.3538098378450074 0.0 64-65 9.514853780960264E-5 7.61188302476821E-5 9.514853780960263E-6 0.40183130487751384 0.0 66-67 9.514853780960264E-5 8.563368402864237E-5 9.514853780960263E-6 0.45922014545737566 0.0 68-69 1.1417824537152317E-4 8.563368402864237E-5 9.514853780960263E-6 0.5276604887038229 0.0 70-71 1.1417824537152317E-4 8.563368402864237E-5 9.514853780960263E-6 0.6035937793027762 0.0 72-73 1.1417824537152317E-4 1.0942081848104302E-4 9.514853780960263E-6 0.6831998034611804 0.0 74-75 1.1417824537152317E-4 1.4272280671440396E-4 9.514853780960263E-6 0.7684624081923652 0.0 76-77 1.1417824537152317E-4 1.4272280671440396E-4 9.514853780960263E-6 0.8633683172305534 0.0 78-79 1.1417824537152317E-4 1.4272280671440396E-4 9.514853780960263E-6 0.9758576760559561 0.0 80-81 1.1417824537152317E-4 1.474802336048841E-4 9.514853780960263E-6 1.102438533330961 0.0 82-83 1.1417824537152317E-4 1.522376604953642E-4 9.514853780960263E-6 1.2373020708222917 0.0 84-85 1.1417824537152317E-4 1.522376604953642E-4 9.514853780960263E-6 1.3757669804697161 0.0 86-87 1.1417824537152317E-4 1.522376604953642E-4 9.514853780960263E-6 1.5299171265750533 0.0 88-89 1.1417824537152317E-4 1.522376604953642E-4 9.514853780960263E-6 1.70546617883377 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 20990 0.0 60.26722 1 GTATCAA 40805 0.0 43.294746 1 ATCAACG 50075 0.0 34.91728 3 TCAACGC 50375 0.0 34.681213 4 CAACGCA 52100 0.0 33.531662 5 GTGGTAT 7960 0.0 33.47898 1 TATCAAC 52790 0.0 33.05692 2 AACGCAG 54470 0.0 32.23853 6 TGGTATC 8630 0.0 30.050848 2 ACGCAGA 61200 0.0 28.423998 7 CGCAGAG 61685 0.0 28.015562 8 GCAGAGT 64780 0.0 25.936436 9 GTACATG 37400 0.0 23.370533 1 CAGAGTA 62855 0.0 22.313374 10-11 TACATGG 36610 0.0 22.289396 2 GAGTACT 44275 0.0 21.978642 12-13 AGAGTAC 60785 0.0 21.682207 10-11 GTACTTT 45630 0.0 21.094738 14-15 AGTACTT 44675 0.0 20.803612 12-13 ACATGGG 38190 0.0 20.385662 3 >>END_MODULE