FastQCFastQC Report
Wed 25 May 2016
SRR2049408_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049408_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10509883
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT446240.4245908351215708No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT382780.36420957302759693No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258250.2457210988932988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA198300.060.4876751
GTATCAA389300.043.7749981
ATCAACG502350.033.6606673
TCAACGC506700.033.2873274
CAACGCA525800.032.1865435
TATCAAC529700.032.040152
GTGGTAT77500.031.8248181
AACGCAG550550.030.8419556
TGGTATC82350.028.6671352
ACGCAGA630050.026.7463787
CGCAGAG635500.026.4050228
GCAGAGT676650.024.3359459
TATAACG11100.023.5359232
GTACATG358850.021.8907261
AGAGTAC634500.021.64087910-11
TACATGG352250.020.94172
AGTACTT471450.020.71323812-13
GAGTACT462800.020.67447312-13
CAGAGTA659100.020.6566110-11
GTACTTT475450.019.98468614-15
Produced by FastQC (version 0.11.5)