##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049408_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10509883 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06065424324895 33.0 31.0 34.0 30.0 34.0 2 32.24630435943007 34.0 31.0 34.0 30.0 34.0 3 32.31145161178293 34.0 31.0 34.0 30.0 34.0 4 35.952502515965215 37.0 35.0 37.0 35.0 37.0 5 35.841761321224986 37.0 35.0 37.0 35.0 37.0 6 35.87095679371502 37.0 35.0 37.0 35.0 37.0 7 35.799124309947125 37.0 35.0 37.0 35.0 37.0 8 35.793259924967764 37.0 35.0 37.0 35.0 37.0 9 37.39984898024079 39.0 37.0 39.0 34.0 39.0 10-11 37.38735945014802 39.0 37.0 39.0 34.0 39.0 12-13 37.36957081253902 39.0 37.0 39.0 34.0 39.0 14-15 38.58642298872404 40.0 38.0 41.0 34.0 41.0 16-17 38.57704819359074 40.0 38.0 41.0 34.0 41.0 18-19 38.621140311457324 40.0 38.0 41.0 34.0 41.0 20-21 38.61133920330036 40.0 38.0 41.0 34.0 41.0 22-23 38.53202357247935 40.0 38.0 41.0 34.0 41.0 24-25 38.43868109664018 40.0 38.0 41.0 34.0 41.0 26-27 38.263085849766355 40.0 38.0 41.0 34.0 41.0 28-29 38.08452548900877 40.0 38.0 41.0 33.0 41.0 30-31 37.897670602041906 40.0 37.0 41.0 33.0 41.0 32-33 37.65381841072826 40.0 37.0 41.0 33.0 41.0 34-35 37.47924786603238 40.0 36.5 41.0 32.5 41.0 36-37 37.2949732646881 39.0 36.0 41.0 32.0 41.0 38-39 37.447771920962396 40.0 37.0 41.0 33.0 41.0 40-41 37.36189313430035 40.0 37.0 41.0 32.0 41.0 42-43 37.17210905202275 40.0 36.0 41.0 31.5 41.0 44-45 36.933948741389415 40.0 36.0 41.0 31.0 41.0 46-47 36.6052545018817 39.5 36.0 41.0 30.5 41.0 48-49 36.330532033515496 39.0 36.0 41.0 30.0 41.0 50-51 36.104654067033856 39.0 35.0 41.0 29.5 41.0 52-53 35.925183277492245 39.0 35.0 40.0 29.0 41.0 54-55 35.81685581085917 39.0 35.0 40.0 29.0 41.0 56-57 35.72676793833004 39.0 35.0 40.0 28.5 41.0 58-59 35.49318755498991 38.5 35.0 40.0 28.0 41.0 60-61 35.250583807640865 38.0 35.0 40.0 28.0 41.0 62-63 34.97659469662983 38.0 34.0 40.0 27.5 41.0 64-65 34.722207278615755 37.5 34.0 40.0 27.0 41.0 66-67 34.38358628730691 37.0 34.0 40.0 26.0 41.0 68-69 34.06254936425077 36.5 34.0 39.5 26.0 41.0 70-71 33.73406516514028 36.0 33.5 39.0 26.0 41.0 72-73 33.37019151402542 35.5 33.0 39.0 25.5 40.0 74-75 32.992788977765024 35.0 33.0 38.0 25.0 40.0 76-77 30.988260858850666 33.5 30.0 35.5 22.0 38.5 78-79 32.30871804186593 35.0 32.5 37.0 24.5 39.0 80-81 32.32180305908258 35.0 33.0 36.5 25.0 39.0 82-83 32.053522241874624 35.0 33.0 36.0 24.0 38.5 84-85 31.789091943269014 35.0 33.0 36.0 24.0 37.0 86-87 31.527841699094083 35.0 32.0 35.5 23.5 37.0 88-89 31.265798772450655 35.0 32.0 35.0 22.5 36.5 90-91 31.000477645659807 35.0 32.0 35.0 20.5 36.0 92-93 30.76534377214285 34.5 32.0 35.0 19.5 36.0 94-95 30.513227407003484 34.0 32.0 35.0 18.0 36.0 96-97 30.272630532613924 34.0 31.0 35.0 12.0 35.0 98-99 29.99280234613459 34.0 31.0 35.0 2.0 35.0 100-101 29.38610444093431 34.0 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.35645675133469723 1101 2 0.36140816145088195 1101 3 0.3711511718186742 1101 4 0.29551900976886714 1101 5 0.2773502036554092 1101 6 0.30515336211364286 1101 7 0.33902378585815285 1101 8 0.35239622084865374 1101 9 0.411248182615374 1101 10-11 0.38079707481625746 1101 12-13 0.3525993001149885 1101 14-15 0.4026515235251864 1101 16-17 0.4272196067315761 1101 18-19 0.3694917211483997 1101 20-21 0.31684209227083926 1101 22-23 0.31092332198117134 1101 24-25 0.3122029190339006 1101 26-27 0.2571616943259585 1101 28-29 0.281473634311304 1101 30-31 0.2975658661907943 1101 32-33 0.32122759625322317 1101 34-35 0.26482978673036683 1101 36-37 0.3169396838643195 1101 38-39 0.41973580429131374 1101 40-41 0.437549198393377 1101 42-43 0.4786890365667986 1101 44-45 0.45864444572096374 1101 46-47 0.4874355931556451 1101 48-49 0.4205976834865126 1101 50-51 0.4825604023294616 1101 52-53 0.46960437316936066 1101 54-55 0.38421373396201375 1101 56-57 0.35440551194299985 1101 58-59 0.3184634625278804 1101 60-61 0.3393763252136637 1101 62-63 0.3024528890878315 1101 64-65 0.2287525431284294 1101 66-67 0.3030680035319122 1101 68-69 0.2812943775596253 1101 70-71 0.3098476321294825 1101 72-73 0.22093078754392081 1101 74-75 0.17764109389080573 1101 76-77 0.17514758160131905 1101 78-79 0.20556617032400482 1101 80-81 0.23868536107007543 1101 82-83 0.17040334839470006 1101 84-85 0.11766827740472152 1101 86-87 0.12269678248104654 1101 88-89 0.12354331014718056 1101 90-91 0.12424406988299452 1101 92-93 0.13071829713171113 1101 94-95 0.10044819932899784 1101 96-97 0.027490259941970407 1101 98-99 -0.012080730121233785 1101 100-101 -0.028611375811951945 1102 1 0.3637039944870537 1102 2 0.3570900211143666 1102 3 0.32285094178328677 1102 4 0.2686029832732757 1102 5 0.28955331750317015 1102 6 0.2790882672599224 1102 7 0.31292006321744736 1102 8 0.3143957988332531 1102 9 0.3802507533732111 1102 10-11 0.3280723674095043 1102 12-13 0.2989165520406445 1102 14-15 0.35081373948091965 1102 16-17 0.37671417536628127 1102 18-19 0.35190700247039075 1102 20-21 0.3358359343538737 1102 22-23 0.2974265208994993 1102 24-25 0.3279777992663355 1102 26-27 0.2783603187926218 1102 28-29 0.30098102919770753 1102 30-31 0.2704623871320422 1102 32-33 0.2915605285184668 1102 34-35 0.26876927496049063 1102 36-37 0.2315441346195044 1102 38-39 0.348356681298732 1102 40-41 0.32658597060616046 1102 42-43 0.4299248917994234 1102 44-45 0.40962313855646926 1102 46-47 0.4111105459109794 1102 48-49 0.22204166510359613 1102 50-51 0.22345789801273241 1102 52-53 0.30348370175158124 1102 54-55 0.2606096170175576 1102 56-57 0.24477164772378757 1102 58-59 0.2029287778395954 1102 60-61 0.2709999454341485 1102 62-63 0.23741985112667408 1102 64-65 0.26051024686531576 1102 66-67 0.18855780721303717 1102 68-69 0.1456388900456531 1102 70-71 0.14178302180359026 1102 72-73 0.10062659263874707 1102 74-75 0.07928973028707986 1102 76-77 0.08532621341565161 1102 78-79 0.17916070281353313 1102 80-81 0.17767904630715492 1102 82-83 0.20836567925091742 1102 84-85 0.1678262893429583 1102 86-87 0.09568712384909972 1102 88-89 0.0591439495928654 1102 90-91 0.009224114813431328 1102 92-93 0.02043830950653458 1102 94-95 0.0056293482572122855 1102 96-97 0.01199342822544125 1102 98-99 0.06809456509207834 1102 100-101 0.07841551761027077 1103 1 0.3365249851029475 1103 2 0.38161718679667445 1103 3 0.30432074284777855 1103 4 0.28074764028863797 1103 5 0.253963430750197 1103 6 0.29036023155573787 1103 7 0.2583866481953834 1103 8 0.3183343974416104 1103 9 0.3731567321869065 1103 10-11 0.33947396533397267 1103 12-13 0.29929006300461225 1103 14-15 0.4080749299627726 1103 16-17 0.4259898556089823 1103 18-19 0.3566840804002922 1103 20-21 0.3409884639522005 1103 22-23 0.2992028083248357 1103 24-25 0.3062101795970733 1103 26-27 0.3161194788361996 1103 28-29 0.3136804834010505 1103 30-31 0.3382770334086658 1103 32-33 0.3522989999469033 1103 34-35 0.31076591566894507 1103 36-37 0.32080325291757816 1103 38-39 0.37911050899219134 1103 40-41 0.4376759511100943 1103 42-43 0.43470614970812704 1103 44-45 0.43260299279572223 1103 46-47 0.5403287145308866 1103 48-49 0.5173816102948479 1103 50-51 0.4883361797439534 1103 52-53 0.44649429403503405 1103 54-55 0.42380836697589785 1103 56-57 0.4044127742856176 1103 58-59 0.3889648963308119 1103 60-61 0.36177231106221797 1103 62-63 0.40370133545995657 1103 64-65 0.38668256806618473 1103 66-67 0.32700496191231565 1103 68-69 0.28762465466637366 1103 70-71 0.31238421567068286 1103 72-73 0.3546015760626844 1103 74-75 0.3129169704164454 1103 76-77 0.2785367091144515 1103 78-79 0.40417359017678933 1103 80-81 0.37058335855787305 1103 82-83 0.4222641522340922 1103 84-85 0.3912903859849415 1103 86-87 0.38109496438193347 1103 88-89 0.33502674483263917 1103 90-91 0.38914762771539557 1103 92-93 0.301230557719542 1103 94-95 0.33232305667253215 1103 96-97 0.31819932334185097 1103 98-99 0.2939944901021718 1103 100-101 0.30817737661730504 1104 1 0.32246312798286425 1104 2 0.29164597827073635 1104 3 0.28908188678913405 1104 4 0.24522248386546863 1104 5 0.2608509137247381 1104 6 0.25191668205405904 1104 7 0.31080582040656424 1104 8 0.2747100478988642 1104 9 0.32301847604609435 1104 10-11 0.3009631678417506 1104 12-13 0.2690112044681925 1104 14-15 0.3452438144872545 1104 16-17 0.3580423639855468 1104 18-19 0.3261884558154904 1104 20-21 0.3213500619129519 1104 22-23 0.3388466787712616 1104 24-25 0.30425343808678207 1104 26-27 0.2866004218269751 1104 28-29 0.3131519661693858 1104 30-31 0.28515454161816933 1104 32-33 0.3327526528757758 1104 34-35 0.3209029270855126 1104 36-37 0.2623567541566203 1104 38-39 0.35741973063276333 1104 40-41 0.3638597597032174 1104 42-43 0.3817062078341493 1104 44-45 0.47532690606448114 1104 46-47 0.540625218239974 1104 48-49 0.5423301507323757 1104 50-51 0.5614015192280419 1104 52-53 0.5340130457765611 1104 54-55 0.5182668143470792 1104 56-57 0.45527545781349943 1104 58-59 0.51967518148669 1104 60-61 0.5785089782199861 1104 62-63 0.5814496277163244 1104 64-65 0.561237548654951 1104 66-67 0.5764559335131878 1104 68-69 0.5783940141059318 1104 70-71 0.5613622371393348 1104 72-73 0.5259226867024935 1104 74-75 0.5087756556588303 1104 76-77 0.4239984482429513 1104 78-79 0.517559125027951 1104 80-81 0.5477517694825522 1104 82-83 0.5126269954494234 1104 84-85 0.47200127308458306 1104 86-87 0.4548897960662579 1104 88-89 0.4451007404271543 1104 90-91 0.4557691989827255 1104 92-93 0.3831892052966879 1104 94-95 0.3696829482950754 1104 96-97 0.2807260965806684 1104 98-99 0.2977975283748684 1104 100-101 0.355391763847571 1105 1 0.33666364659445946 1105 2 0.3135305609902943 1105 3 0.28994067183082706 1105 4 0.21179079831247094 1105 5 0.21023380789790025 1105 6 0.24370545776170616 1105 7 0.25585645013289593 1105 8 0.2660695353155802 1105 9 0.3167533719908917 1105 10-11 0.3071286758669274 1105 12-13 0.28347958094155246 1105 14-15 0.36445970305977227 1105 16-17 0.30432556026999436 1105 18-19 0.3640636278241729 1105 20-21 0.3036159492907302 1105 22-23 0.2362795879487578 1105 24-25 0.2640389428163914 1105 26-27 0.315542759760163 1105 28-29 0.32646267830978815 1105 30-31 0.2916194413983675 1105 32-33 0.35259929314292293 1105 34-35 0.3402081754414823 1105 36-37 0.30618179117496425 1105 38-39 0.296879120098545 1105 40-41 0.323961480633848 1105 42-43 0.3665608971264547 1105 44-45 0.4202653539981327 1105 46-47 0.4162004273310558 1105 48-49 0.34274713294426107 1105 50-51 0.37865471240062476 1105 52-53 0.413937734622678 1105 54-55 0.4120679405878249 1105 56-57 0.4145904122331956 1105 58-59 0.3424239014542607 1105 60-61 0.3795588966118615 1105 62-63 0.39090462936312065 1105 64-65 0.4159493719957297 1105 66-67 0.48118233123661724 1105 68-69 0.45751806517612437 1105 70-71 0.3851883079447518 1105 72-73 0.4314578738500501 1105 74-75 0.38524018047189656 1105 76-77 0.42552077397445487 1105 78-79 0.4573613874964124 1105 80-81 0.4426884806851845 1105 82-83 0.48863389793680767 1105 84-85 0.48587889175162147 1105 86-87 0.480751931448399 1105 88-89 0.48578546028896596 1105 90-91 0.563358247965585 1105 92-93 0.5391021889839855 1105 94-95 0.531025429124 1105 96-97 0.5637344110620681 1105 98-99 0.5061472985492017 1105 100-101 0.609249936338724 1106 1 0.2762125721161297 1106 2 0.2572986662806471 1106 3 0.24851602084944346 1106 4 0.22535970730845634 1106 5 0.2240717630656448 1106 6 0.21869233396979126 1106 7 0.28117865573783973 1106 8 0.25992169309857616 1106 9 0.3337664945204608 1106 10-11 0.2649419100299255 1106 12-13 0.24507875611090668 1106 14-15 0.2873709881199673 1106 16-17 0.30890030509845445 1106 18-19 0.2905446991228757 1106 20-21 0.23241562948248884 1106 22-23 0.25111870590335883 1106 24-25 0.2660726210222464 1106 26-27 0.2630058382772589 1106 28-29 0.3399174502246751 1106 30-31 0.34080441453806287 1106 32-33 0.3122905951180144 1106 34-35 0.3251428039542503 1106 36-37 0.35659167785867396 1106 38-39 0.3739390377794152 1106 40-41 0.36398092180800745 1106 42-43 0.3960516133504015 1106 44-45 0.416331793070313 1106 46-47 0.46523547939846566 1106 48-49 0.425407661642474 1106 50-51 0.46107973913144207 1106 52-53 0.4341504342454314 1106 54-55 0.4462765244554916 1106 56-57 0.409167086258833 1106 58-59 0.45579413390915846 1106 60-61 0.4712319373202263 1106 62-63 0.5094811125641883 1106 64-65 0.5037491068628341 1106 66-67 0.49333366995806216 1106 68-69 0.4610689512958359 1106 70-71 0.39937433737842554 1106 72-73 0.3729720917580934 1106 74-75 0.3718170110283907 1106 76-77 0.41093082463159547 1106 78-79 0.47545095770166057 1106 80-81 0.4967508986080631 1106 82-83 0.4795116582888568 1106 84-85 0.45392349396315623 1106 86-87 0.41942045419555996 1106 88-89 0.4334882661246908 1106 90-91 0.44445004401047683 1106 92-93 0.440481820513547 1106 94-95 0.5138051723378325 1106 96-97 0.47974488228892653 1106 98-99 0.5754771553009412 1106 100-101 0.642351723638626 1107 1 0.21868096633050982 1107 2 0.20741929470237608 1107 3 0.16027162039722498 1107 4 0.17871964626296943 1107 5 0.15174168826663248 1107 6 0.14569115483981676 1107 7 0.17531879461923694 1107 8 0.1288182392078241 1107 9 0.23946540025524854 1107 10-11 0.19252708199135782 1107 12-13 0.18419248854763026 1107 14-15 0.16672780800201537 1107 16-17 0.17018199185938698 1107 18-19 0.12918900418151935 1107 20-21 0.15127901625923812 1107 22-23 0.14311691630316403 1107 24-25 0.2095904144391767 1107 26-27 0.21447671223265985 1107 28-29 0.2260392941210867 1107 30-31 0.24543120669129337 1107 32-33 0.24395096335471322 1107 34-35 0.2579518786926229 1107 36-37 0.2576400698981658 1107 38-39 0.26245661013980026 1107 40-41 0.30010420578583563 1107 42-43 0.28282432892346066 1107 44-45 0.18957086104962428 1107 46-47 0.17833617713600347 1107 48-49 0.11968119131062593 1107 50-51 0.15087499446034514 1107 52-53 0.0999395536952008 1107 54-55 0.1937372497148786 1107 56-57 0.21730380931650473 1107 58-59 0.277693127169222 1107 60-61 0.31324008185539043 1107 62-63 0.276287754463155 1107 64-65 0.30980570191765366 1107 66-67 0.32172636193183024 1107 68-69 0.3477902828229489 1107 70-71 0.3213079922019588 1107 72-73 0.33315938730792993 1107 74-75 0.2937595115190206 1107 76-77 0.32635179107785106 1107 78-79 0.4589630301259362 1107 80-81 0.43496029993119123 1107 82-83 0.43629569197383944 1107 84-85 0.43597892169641383 1107 86-87 0.44674073120507884 1107 88-89 0.38519819870290917 1107 90-91 0.4451079096385264 1107 92-93 0.4757804763288682 1107 94-95 0.4950324149117442 1107 96-97 0.523418541638474 1107 98-99 0.5818621391484875 1107 100-101 0.6912411935547453 1108 1 0.14293173852604468 1108 2 0.15971743858535348 1108 3 0.11732587289543517 1108 4 0.1272125846932326 1108 5 0.0968819702544792 1108 6 0.11746662006125774 1108 7 0.13223345968808076 1108 8 0.13081889582863937 1108 9 0.2112165149033487 1108 10-11 0.11782583384226797 1108 12-13 0.11740971381017573 1108 14-15 0.2026822500823542 1108 16-17 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-1.0025317937484672 2316 98-99 -0.9892843973409633 2316 100-101 -1.2562555902680863 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 25.0 4 77.0 5 170.0 6 410.0 7 1252.0 8 3227.0 9 6616.0 10 10970.0 11 16469.0 12 22910.0 13 31948.0 14 40734.0 15 52698.0 16 68127.0 17 91013.0 18 77533.0 19 46164.0 20 37078.0 21 35982.0 22 37602.0 23 40992.0 24 48837.0 25 60534.0 26 76562.0 27 98112.0 28 124960.0 29 159994.0 30 202903.0 31 257839.0 32 332302.0 33 441379.0 34 616304.0 35 921715.0 36 1437307.0 37 2236618.0 38 2344385.0 39 527964.0 40 166.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.114635961111105 22.420804158790748 16.746095421887873 22.718464458210278 2 14.329155997502866 21.9147851655179 39.62472010245062 24.131338734528622 3 16.389077944287138 27.120236600835423 31.918981169384576 24.57170428549286 4 12.07232295621882 19.01309227964831 38.743520607033894 30.171064157098982 5 12.369360971204488 38.32683656801422 35.62871012367848 13.675092337102813 6 28.870108797473115 39.45463390953377 17.844848445404445 13.830408847588673 7 25.624072123352846 33.91974962994355 22.86781879493806 17.58835945176554 8 25.152699145432837 36.00793044108392 20.196420854201786 18.642949559281448 9 24.827129231045014 16.973048392397473 21.929311426568958 36.270510949988555 10-11 23.421425989504545 27.74581843505174 28.746395891637594 20.086359683806123 12-13 24.376717609510973 25.365458397586348 29.06812568703191 21.18969830587077 14-15 21.67600426876309 27.006891449114907 27.203716594626215 24.11338768749579 16-17 20.335621243357323 30.386023326805827 27.889278120412946 21.3890773094239 18-19 20.33988978463355 28.492677483633848 31.09004838543551 20.07738434629709 20-21 21.325471045759194 27.536306831121536 31.083679869904696 20.05454225321457 22-23 20.987350667937978 27.376998392846048 31.19294953140772 20.44270140780825 24-25 20.70484260841157 27.69242560399337 31.00121158099593 20.601520206599133 26-27 20.4989765348801 27.75255452355026 30.988638806853245 20.759830134716395 28-29 20.622255395457373 27.64941360814297 31.220128685107152 20.508202311292507 30-31 20.840057634008442 27.838057684818324 30.911122958292037 20.4107617228812 32-33 20.56886070283454 27.77590164670704 31.053453464083336 20.601784186375085 34-35 20.828849120142657 27.630261040680022 30.9355948041172 20.605295035060127 36-37 21.06630502294489 27.699476350865137 30.57651360005747 20.657705026132504 38-39 20.83108361061478 27.952343891128027 30.66252532142311 20.554047176834082 40-41 20.718506889384443 27.787021369387578 30.71492590618928 20.779545835038704 42-43 20.685214472775094 27.9411076969832 30.524596635670243 20.849081194571465 44-45 20.80738115288553 28.05648102824465 30.254178614053778 20.88195920481604 46-47 21.06528039550042 28.054945161072858 29.85149752024879 21.028276923177927 48-49 21.048202075193213 28.19953848226662 29.596932235373284 21.155327207166884 50-51 21.141413718525595 28.30080791799599 29.256736376671906 21.301041986806514 52-53 21.107193179910304 28.44369366401271 29.16955944278483 21.279553713292152 54-55 21.119962359003853 28.74148861290883 29.134773272704308 21.003775755383003 56-57 21.215262273161887 28.409699536521078 29.133334088179836 21.241704102137202 58-59 21.141888631742624 28.136998623855696 29.26780997429161 21.453302770110067 60-61 21.24848116285108 28.370381644363903 29.267436992671282 21.11370020011374 62-63 21.010486688895845 28.452642719629246 29.2249978091478 21.311872782327107 64-65 21.12518671275511 28.43561103270012 29.18381793134185 21.255384323202925 66-67 21.075996558482675 28.37662729398806 29.193768805930127 21.35360734159914 68-69 21.222173412586056 28.55142478436389 28.973469279879506 21.252932523170557 70-71 21.304085623549902 28.33631091935474 29.00849430313069 21.351109153964668 72-73 21.082760512514426 28.531990666643452 28.970855953820763 21.414392867021363 74-75 21.12695964503437 28.645763183672106 28.840186822914777 21.38709034837875 76-77 21.184738697885848 28.664305417865204 28.712589416265537 21.438366467983414 78-79 21.165735615811403 28.690577118319105 28.818312347818143 21.32537491805135 80-81 21.140767689861768 28.7094338983525 28.729448568482475 21.42034984330326 82-83 21.014201274583268 28.65495008904252 28.906889540414237 21.423959095959972 84-85 20.997639685108027 28.685116733373285 28.756335796789795 21.56090778472889 86-87 21.04554967275844 28.767935674877315 28.693202308570203 21.493312343794038 88-89 20.945730345177186 28.695875985794896 28.762619708975674 21.59577396005225 90-91 20.816830416076186 28.63480776626826 28.835012185818172 21.713349631837385 92-93 20.875317861818225 28.771478553000662 28.710288473024914 21.642915112156203 94-95 20.832263501820794 28.515920686652237 28.853152512183232 21.798663299343733 96-97 20.916083047768023 28.69008659015752 28.712969874465443 21.68086048760901 98-99 20.700951828544028 28.663392872392198 28.700496254765838 21.935159044297937 100-101 20.604544313195493 28.7150675226356 28.701708667927132 21.978679496241778 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5001.0 1 3399.0 2 2525.5 3 3298.0 4 2632.0 5 2174.0 6 4116.0 7 9542.5 8 15810.0 9 18611.5 10 15740.5 11 11752.0 12 11304.0 13 12218.5 14 13193.5 15 14054.5 16 14944.0 17 15997.0 18 17254.0 19 19083.5 20 21875.0 21 25752.5 22 32101.5 23 42209.0 24 55138.0 25 70217.0 26 90250.5 27 115493.5 28 143372.5 29 172572.0 30 197732.5 31 220460.0 32 245742.0 33 268072.5 34 286574.0 35 306202.0 36 324920.0 37 342890.0 38 363471.0 39 375516.0 40 383737.0 41 390466.0 42 392184.0 43 394069.5 44 387396.0 45 376286.5 46 368182.5 47 358728.5 48 347724.0 49 336944.5 50 323790.5 51 303004.0 52 272630.0 53 246652.0 54 228231.0 55 211362.5 56 190537.5 57 166416.5 58 147920.5 59 134796.5 60 120008.0 61 102085.0 62 85738.5 63 70742.5 64 58148.0 65 47815.5 66 38217.5 67 30037.0 68 23174.0 69 17394.5 70 12780.5 71 9159.0 72 6781.5 73 4961.0 74 3587.0 75 2519.5 76 1595.5 77 1067.5 78 755.0 79 494.0 80 330.0 81 206.5 82 144.5 83 111.5 84 80.5 85 53.5 86 32.5 87 26.0 88 20.5 89 17.0 90 12.5 91 6.5 92 3.0 93 2.0 94 2.0 95 1.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05034309135506076 2 0.002226475784744702 3 2.854456134288079E-4 4 2.3787134452400659E-4 5 0.0027973670116023175 6 0.0016365548503251654 7 0.0 8 9.514853780960265E-6 9 8.563368402864237E-5 10-11 1.902970756192053E-5 12-13 0.0 14-15 4.471981277051324E-4 16-17 0.0 18-19 5.233169579528145E-5 20-21 4.7098526215753303E-4 22-23 0.0 24-25 5.851635075290562E-4 26-27 0.006960115540772433 28-29 0.007055264078582036 30-31 0.0011037230385913907 32-33 0.006346407471900496 34-35 0.00754527904830149 36-37 0.004381590166132201 38-39 0.0024215302872543872 40-41 0.0018982133293015727 42-43 0.0011655695881676322 44-45 8.420645596149834E-4 46-47 5.566189461861755E-4 48-49 0.002007634147782616 50-51 0.0014557726284869204 52-53 0.0021551143813874998 54-55 6.232229226528973E-4 56-57 1.9029707561920526E-4 58-59 7.992477176006622E-4 60-61 0.005799303379495281 62-63 0.0026070699359831122 64-65 8.515794133959435E-4 66-67 0.0053758923862425485 68-69 0.007626155305439652 70-71 0.0023882282990210263 72-73 4.947723966099338E-4 74-75 0.0034110750804742544 76-77 0.0018078222183824503 78-79 0.0024072580065829467 80-81 8.753665478483443E-4 82-83 1.5699508738584435E-4 84-85 5.375892386242549E-4 86-87 0.004957238819880297 88-89 0.002668916485559354 90-91 0.001189356722620033 92-93 9.039111091912252E-5 94-95 4.8050011593849337E-4 96-97 2.6641590586688735E-4 98-99 5.661337999671357E-4 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.0509883E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.053667485658686 #Duplication Level Percentage of deduplicated Percentage of total 1 65.22627253782309 28.08230249172386 2 18.26862266462185 15.730624112471947 3 6.86105446656085 8.861806728129126 4 3.1925196055613156 5.497987101571325 5 1.753865796082431 3.775517739950153 6 1.0714586110897222 2.7678133659901554 7 0.6789294130671893 2.0461280837499727 8 0.47027928658927515 1.6197798424165952 9 0.3485130795971016 1.3504289619038858 >10 1.8922280788588424 14.76966771250928 >50 0.13840688461092743 4.137200224084413 >100 0.09007613191591411 7.276197281534763 >500 0.005821107530442444 1.6637610834583656 >1k 0.0018414082921851887 1.2323249047750764 >5k 4.4371119600803334E-5 0.1499329901054224 >10k+ 6.6556679401205E-5 1.0385273756254227 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 44624 0.4245908351215708 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 38278 0.36420957302759693 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25825 0.2457210988932988 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.9029707561920527E-5 0.0 0.0 0.0 6 0.0 1.9029707561920527E-5 0.0 0.0 0.0 7 0.0 1.9029707561920527E-5 0.0 0.0 0.0 8 0.0 1.9029707561920527E-5 0.0 1.9029707561920527E-5 0.0 9 0.0 1.9029707561920527E-5 0.0 2.854456134288079E-5 0.0 10-11 4.757426890480132E-6 1.9029707561920527E-5 0.0 3.805941512384106E-5 0.0 12-13 1.4272280671440396E-5 1.9029707561920527E-5 0.0 4.757426890480132E-5 0.0 14-15 2.854456134288079E-5 2.3787134452400657E-5 0.0 5.708912268576158E-5 0.0 16-17 3.330198823336092E-5 4.757426890480132E-5 0.0 7.136140335720198E-5 0.0 18-19 4.281684201432119E-5 5.708912268576158E-5 0.0 8.087625713816223E-5 0.0 20-21 4.757426890480132E-5 5.708912268576158E-5 0.0 9.039111091912252E-5 0.0 22-23 4.757426890480132E-5 6.184654957624172E-5 0.0 1.474802336048841E-4 0.0 24-25 7.61188302476821E-5 6.660397646672185E-5 0.0 2.7117333275736755E-4 0.0 26-27 7.61188302476821E-5 6.660397646672185E-5 0.0 4.805001159384933E-4 0.0 28-29 8.087625713816223E-5 7.136140335720198E-5 0.0 0.0011608121612771522 0.0 30-31 8.563368402864237E-5 8.563368402864237E-5 0.0 0.0026070699359831122 0.0 32-33 8.563368402864237E-5 8.563368402864237E-5 0.0 0.005604248876985595 0.0 34-35 8.563368402864237E-5 8.563368402864237E-5 0.0 0.01046633915905629 0.0 36-37 9.039111091912252E-5 8.563368402864237E-5 0.0 0.018558722299762992 0.0 38-39 9.514853780960264E-5 1.1417824537152317E-4 0.0 0.030195388473877396 0.0 40-41 9.514853780960264E-5 1.1417824537152317E-4 0.0 0.04236964388661606 9.514853780960263E-6 42-43 9.990596470008277E-5 1.1417824537152317E-4 0.0 0.055252755906036254 9.514853780960263E-6 44-45 1.046633915905629E-4 1.2369309915248342E-4 0.0 0.07198462627985487 9.514853780960263E-6 46-47 1.046633915905629E-4 1.332079529334437E-4 0.0 0.09306478483157235 9.514853780960263E-6 48-49 1.046633915905629E-4 1.332079529334437E-4 0.0 0.11619539437308674 9.514853780960263E-6 50-51 1.1417824537152317E-4 1.4272280671440396E-4 0.0 0.14092449934980247 9.514853780960263E-6 52-53 1.1417824537152317E-4 1.4272280671440396E-4 0.0 0.16489241602404137 9.514853780960263E-6 54-55 1.1417824537152317E-4 1.4272280671440396E-4 0.0 0.19269957619889774 9.514853780960263E-6 56-57 1.1893567226200329E-4 1.617525142763245E-4 0.0 0.2266628467700354 9.514853780960263E-6 58-59 1.4272280671440396E-4 1.617525142763245E-4 0.0 0.2659972523005252 9.514853780960263E-6 60-61 1.4272280671440396E-4 1.617525142763245E-4 0.0 0.30806242086615043 9.514853780960263E-6 62-63 1.4272280671440396E-4 1.665099411668046E-4 0.0 0.35180696112411525 9.514853780960263E-6 64-65 1.4272280671440396E-4 1.7126736805728475E-4 0.0 0.3997047350574693 9.514853780960263E-6 66-67 1.4272280671440396E-4 1.80782221838245E-4 0.0 0.4566321052289545 9.514853780960263E-6 68-69 1.4272280671440396E-4 1.9029707561920529E-4 0.0 0.5245063146754345 9.514853780960263E-6 70-71 1.617525142763245E-4 1.950545025096854E-4 0.0 0.5997117189601444 9.514853780960263E-6 72-73 1.617525142763245E-4 2.0456935629064568E-4 0.0 0.6785470399622908 9.514853780960263E-6 74-75 1.665099411668046E-4 2.235990638525662E-4 0.0 0.7630151544027655 9.514853780960263E-6 76-77 1.7126736805728475E-4 2.664159058668874E-4 0.0 0.8573073553720817 9.514853780960263E-6 78-79 1.7126736805728475E-4 2.664159058668874E-4 0.0 0.9693733032042318 9.514853780960263E-6 80-81 1.7126736805728475E-4 2.664159058668874E-4 0.0 1.0951596701885262 9.514853780960263E-6 82-83 1.7126736805728475E-4 2.7117333275736755E-4 0.0 1.2291811431202422 9.514853780960263E-6 84-85 1.7126736805728475E-4 2.7593075964784763E-4 0.0 1.3671465229441662 9.514853780960263E-6 86-87 1.7126736805728475E-4 2.7593075964784763E-4 0.0 1.5204498470629977 9.514853780960263E-6 88-89 1.7126736805728475E-4 2.7593075964784763E-4 0.0 1.6949903248209328 9.514853780960263E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 19830 0.0 60.487675 1 GTATCAA 38930 0.0 43.774998 1 ATCAACG 50235 0.0 33.660667 3 TCAACGC 50670 0.0 33.287327 4 CAACGCA 52580 0.0 32.186543 5 TATCAAC 52970 0.0 32.04015 2 GTGGTAT 7750 0.0 31.824818 1 AACGCAG 55055 0.0 30.841955 6 TGGTATC 8235 0.0 28.667135 2 ACGCAGA 63005 0.0 26.746378 7 CGCAGAG 63550 0.0 26.405022 8 GCAGAGT 67665 0.0 24.335945 9 TATAACG 1110 0.0 23.535923 2 GTACATG 35885 0.0 21.890726 1 AGAGTAC 63450 0.0 21.640879 10-11 TACATGG 35225 0.0 20.9417 2 AGTACTT 47145 0.0 20.713238 12-13 GAGTACT 46280 0.0 20.674473 12-13 CAGAGTA 65910 0.0 20.65661 10-11 GTACTTT 47545 0.0 19.984686 14-15 >>END_MODULE