FastQCFastQC Report
Wed 25 May 2016
SRR2049407_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049407_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10899923
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT464990.4265993438669246No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT387590.35558966792701197No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207360.19023987600646353No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA222600.060.2624471
GTATCAA440650.044.380721
ATCAACG534600.036.091943
TCAACGC539300.035.7610974
TATCAAC557000.034.7571682
CAACGCA563850.034.253515
GTGGTAT85300.033.4682271
AACGCAG596350.032.578376
TGGTATC89350.030.3002132
CGCAGAG655450.029.1716798
ACGCAGA666100.028.997677
GCAGAGT697000.026.6420759
GTACATG410750.023.7998941
TACATGG404900.023.0739522
GAGTACT456350.022.88540512-13
CAGAGTA675550.022.84239210-11
AGAGTAC651200.021.89840310-11
ACATGGG412650.021.767523
GTACTTT489750.021.13333914-15
AGTACTT474350.020.51479712-13
Produced by FastQC (version 0.11.5)