##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049407_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10899923 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.50935846060564 31.0 31.0 34.0 30.0 34.0 2 31.551716833229005 33.0 31.0 34.0 30.0 34.0 3 31.570728160189756 33.0 31.0 34.0 28.0 34.0 4 35.19121869026047 37.0 35.0 37.0 33.0 37.0 5 35.21323499257747 37.0 35.0 37.0 33.0 37.0 6 35.30682317664079 37.0 35.0 37.0 33.0 37.0 7 35.257714114127225 37.0 35.0 37.0 33.0 37.0 8 35.23085612623135 37.0 35.0 37.0 33.0 37.0 9 36.81936606341164 39.0 37.0 39.0 33.0 39.0 10-11 36.75766796701224 39.0 37.0 39.0 33.0 39.0 12-13 36.722053999831004 39.0 37.0 39.0 33.0 39.0 14-15 37.89438416216335 40.0 38.0 41.0 33.0 41.0 16-17 37.89043830860089 40.0 38.0 41.0 33.0 41.0 18-19 37.9345119226989 40.0 38.0 41.0 33.0 41.0 20-21 37.91402329172417 40.0 38.0 41.0 33.0 41.0 22-23 37.816357464176576 40.0 38.0 41.0 33.0 41.0 24-25 37.716380565257204 40.0 38.0 41.0 32.0 41.0 26-27 37.5802431815344 40.0 37.5 41.0 32.0 41.0 28-29 37.431540020970786 40.0 37.0 41.0 32.0 41.0 30-31 37.24699775402083 40.0 37.0 41.0 32.0 41.0 32-33 37.00144478084845 40.0 36.5 41.0 31.0 41.0 34-35 36.805896105871575 40.0 36.0 41.0 31.0 41.0 36-37 36.62525950871396 39.5 36.0 41.0 30.0 41.0 38-39 36.43045166465855 39.0 36.0 41.0 30.0 41.0 40-41 36.22249767268998 39.0 35.0 41.0 30.0 41.0 42-43 35.99324137427393 39.0 35.0 40.0 29.5 41.0 44-45 35.708200369855824 39.0 35.0 40.0 28.5 41.0 46-47 35.34196328726359 39.0 35.0 40.0 26.5 41.0 48-49 35.04519054859378 39.0 35.0 40.0 25.5 41.0 50-51 33.70971877507758 37.5 32.5 39.0 23.5 40.0 52-53 33.99444119926352 37.5 33.5 39.5 24.5 40.0 54-55 35.0287585517806 39.0 35.0 40.0 26.0 41.0 56-57 35.15742583686142 39.0 35.0 41.0 26.0 41.0 58-59 35.01703681759954 39.0 35.0 41.0 26.0 41.0 60-61 34.78377746338208 38.5 34.5 40.0 25.5 41.0 62-63 34.53576846368548 38.0 34.0 40.0 25.0 41.0 64-65 34.277300812125006 38.0 34.0 40.0 24.0 41.0 66-67 33.97449848957649 37.5 34.0 40.0 23.0 41.0 68-69 33.64573566253633 37.0 34.0 40.0 22.0 41.0 70-71 33.321444931308235 36.5 33.0 39.0 22.0 41.0 72-73 33.06865053083403 36.0 33.0 39.0 21.5 41.0 74-75 32.86957761077762 36.0 33.0 39.0 21.0 40.5 76-77 32.52245997517598 35.0 33.0 38.5 20.0 40.0 78-79 32.14216403180096 35.0 33.0 37.0 18.5 39.5 80-81 31.789875396367478 35.0 32.5 37.0 18.0 39.0 82-83 31.433379254147024 35.0 32.0 36.5 15.5 39.0 84-85 31.09786477390712 35.0 32.0 36.0 11.0 37.5 86-87 30.789665073780796 35.0 32.0 36.0 7.0 37.0 88-89 30.487988722489142 35.0 32.0 35.0 4.0 37.0 90-91 30.214079493956056 35.0 31.0 35.0 2.0 36.0 92-93 29.960278113891263 35.0 31.0 35.0 2.0 36.0 94-95 29.701617204084837 34.0 31.0 35.0 2.0 36.0 96-97 29.451816815586678 34.0 31.0 35.0 2.0 35.5 98-99 29.14837545182659 34.0 31.0 35.0 2.0 35.0 100-101 28.63223662222201 34.0 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2518114887748233 1101 2 0.2755025962223847 1101 3 0.2439536311027517 1101 4 0.24108692215725114 1101 5 0.24490365940946646 1101 6 0.22849915876476956 1101 7 0.26127545224165516 1101 8 0.23369763882250538 1101 9 0.3333768986673533 1101 10-11 0.2924977750200881 1101 12-13 0.2866400440684842 1101 14-15 0.37204414464288504 1101 16-17 0.33626814713282016 1101 18-19 0.3323851029046807 1101 20-21 0.28055103891122 1101 22-23 0.32829925531552107 1101 24-25 0.2676337271731839 1101 26-27 0.263814122225547 1101 28-29 0.2364047393924693 1101 30-31 0.22975058007690308 1101 32-33 0.18614477195260548 1101 34-35 0.16733779697946005 1101 36-37 0.19350354379934487 1101 38-39 0.1244624965746226 1101 40-41 0.10690611918528248 1101 42-43 0.06494812517588144 1101 44-45 0.06289983302082902 1101 46-47 -0.00446387865428477 1101 48-49 -0.1146054758371946 1101 50-51 -0.14744270777339352 1101 52-53 -0.07198575632506987 1101 54-55 0.1442436376847951 1101 56-57 0.12605733012977538 1101 58-59 0.11109518483043956 1101 60-61 0.1470425405043727 1101 62-63 0.11189366831475667 1101 64-65 0.12981024246133188 1101 66-67 0.10475777011669152 1101 68-69 0.023779783618259387 1101 70-71 -0.05668540903769781 1101 72-73 -0.08259515792603622 1101 74-75 -0.0342897474389261 1101 76-77 -0.03887383317374571 1101 78-79 -0.05686123800246179 1101 80-81 -0.11162678427366757 1101 82-83 -0.139963942410958 1101 84-85 -0.20158030661550086 1101 86-87 -0.279386294768166 1101 88-89 -0.3243133461790748 1101 90-91 -0.29479552375482143 1101 92-93 -0.322274399293768 1101 94-95 -0.4586665249053219 1101 96-97 -0.5168111391995609 1101 98-99 -0.6113924716971688 1101 100-101 -0.7388233814832041 1102 1 0.29738018096434615 1102 2 0.27099272810594144 1102 3 0.3160780706274018 1102 4 0.36247435225946845 1102 5 0.3490630099254659 1102 6 0.3606705460704589 1102 7 0.3562150843341456 1102 8 0.36244632955813216 1102 9 0.3427691831566193 1102 10-11 0.3875148254502889 1102 12-13 0.3511295371607801 1102 14-15 0.4432260515645652 1102 16-17 0.3889397129765726 1102 18-19 0.3905284962584403 1102 20-21 0.38181258945349583 1102 22-23 0.33147679307557354 1102 24-25 0.34280937699755754 1102 26-27 0.3834361671716451 1102 28-29 0.35493717333471864 1102 30-31 0.31953546080831785 1102 32-33 0.29869890880145533 1102 34-35 0.2879884487189628 1102 36-37 0.2979837474020641 1102 38-39 0.21913986699410515 1102 40-41 0.23468958903048076 1102 42-43 0.1095672596581494 1102 44-45 0.2752335833280952 1102 46-47 0.1924714518171058 1102 48-49 0.1103585143935959 1102 50-51 0.06559467715663914 1102 52-53 0.24468113251440826 1102 54-55 0.3510813791933103 1102 56-57 0.37014452069414716 1102 58-59 0.3646267327475172 1102 60-61 0.3650063942321964 1102 62-63 0.35809033195454276 1102 64-65 0.2676006911988651 1102 66-67 0.20569334426722463 1102 68-69 0.22173218567071018 1102 70-71 0.21928550836360472 1102 72-73 0.16363739550963885 1102 74-75 0.15910609021567268 1102 76-77 0.13638634896175006 1102 78-79 0.15196435918472417 1102 80-81 0.0982448824898654 1102 82-83 0.028655223353243997 1102 84-85 0.023507718252211873 1102 86-87 0.006923274428086046 1102 88-89 -0.05034816048082291 1102 90-91 -0.07156527682840164 1102 92-93 -0.2160158800773324 1102 94-95 -0.24167659679866915 1102 96-97 -0.22135471981833987 1102 98-99 -0.20594069306162766 1102 100-101 -0.26944173640551483 1103 1 0.25133479109408796 1103 2 0.24878307337257155 1103 3 0.33471715668152413 1103 4 0.2553011802736478 1103 5 0.3087368552505865 1103 6 0.2682140886785547 1103 7 0.314906160343952 1103 8 0.258285466758835 1103 9 0.35853797340394067 1103 10-11 0.3646803594559671 1103 12-13 0.34726982366324677 1103 14-15 0.39805906341805297 1103 16-17 0.44599078270188386 1103 18-19 0.38281935639158604 1103 20-21 0.4313650058573941 1103 22-23 0.35030257775390083 1103 24-25 0.39846350572370426 1103 26-27 0.3944541745770138 1103 28-29 0.3681092033642912 1103 30-31 0.4152171964457878 1103 32-33 0.28756444430344175 1103 34-35 0.3371200110565695 1103 36-37 0.3356290254571874 1103 38-39 0.37206523719469686 1103 40-41 0.3594244021686208 1103 42-43 0.2642976042718388 1103 44-45 0.23286154840150886 1103 46-47 0.2366945236862108 1103 48-49 0.32625877583969043 1103 50-51 0.1384881623608578 1103 52-53 0.24726765661451822 1103 54-55 0.43698694233621893 1103 56-57 0.4980575633444104 1103 58-59 0.44033096743958566 1103 60-61 0.3870285171689929 1103 62-63 0.4032613329312227 1103 64-65 0.3773339468147867 1103 66-67 0.36438729387151625 1103 68-69 0.26863961988070173 1103 70-71 0.26070648553535847 1103 72-73 0.32355794487288847 1103 74-75 0.29339907374810537 1103 76-77 0.24853707970492422 1103 78-79 0.2856879459203441 1103 80-81 0.2533371979880954 1103 82-83 0.25325982355293064 1103 84-85 0.2116188586849468 1103 86-87 0.22595029573825798 1103 88-89 0.16162755305479592 1103 90-91 0.15943086065252032 1103 92-93 0.06972209639524252 1103 94-95 0.0692068447677272 1103 96-97 0.054661750469030324 1103 98-99 0.0727962720417139 1103 100-101 0.0895672373709786 1104 1 0.00886723693417224 1104 2 0.015781102402620206 1104 3 0.08968997232901899 1104 4 -0.014736401780503172 1104 5 -0.058979909686186716 1104 6 -0.056504087673147296 1104 7 -0.09202167376051307 1104 8 -0.056728021022820485 1104 9 -0.06263191418396019 1104 10-11 -0.007292990691858847 1104 12-13 -0.05330916963880128 1104 14-15 -0.04687203614933111 1104 16-17 -0.012941740807782764 1104 18-19 -0.11576440565555401 1104 20-21 -0.10286454115357202 1104 22-23 -0.048213557474682034 1104 24-25 -0.0414215975851846 1104 26-27 -0.05263947990192719 1104 28-29 -0.10665601319185924 1104 30-31 -0.05286501220978579 1104 32-33 -0.08768854555775363 1104 34-35 -0.12660265843848606 1104 36-37 -0.16452927850647114 1104 38-39 -0.14833394685715717 1104 40-41 -0.21196721953877784 1104 42-43 -0.23505034708862382 1104 44-45 -0.18607506737627233 1104 46-47 -0.17675586382652142 1104 48-49 -0.2905267057952159 1104 50-51 -0.25756135825916715 1104 52-53 -0.2803701651296322 1104 54-55 -0.13655890359406442 1104 56-57 -0.2083757326852549 1104 58-59 -0.18027725634902225 1104 60-61 -0.2585716473568951 1104 62-63 -0.21108473932654448 1104 64-65 -0.19817370322112993 1104 66-67 -0.15396897147449096 1104 68-69 -0.16475408136702185 1104 70-71 -0.19928048601919812 1104 72-73 -0.1985151438708641 1104 74-75 -0.14854164264936287 1104 76-77 -0.17475091157604083 1104 78-79 -0.20314972466042036 1104 80-81 -0.20562031758758081 1104 82-83 -0.18405118047252245 1104 84-85 -0.19739306735356976 1104 86-87 -0.25680068974671855 1104 88-89 -0.26655756779238615 1104 90-91 -0.2971399648164237 1104 92-93 -0.3049767669564112 1104 94-95 -0.35140620205116235 1104 96-97 -0.36144399707978536 1104 98-99 -0.3377267031207225 1104 100-101 -0.3278204340449449 1105 1 0.2472761784982218 1105 2 0.2552874908010878 1105 3 0.23013697262036814 1105 4 0.23433858698902554 1105 5 0.23846009316781647 1105 6 0.2555392423516949 1105 7 0.3048123492564585 1105 8 0.2604025150441558 1105 9 0.309923500562185 1105 10-11 0.2860636940863941 1105 12-13 0.26848125086969077 1105 14-15 0.33385929846488693 1105 16-17 0.29973635513638897 1105 18-19 0.3164378178027647 1105 20-21 0.35233731509766386 1105 22-23 0.3274399163227102 1105 24-25 0.37360576251988675 1105 26-27 0.4013855381560063 1105 28-29 0.3959838386787382 1105 30-31 0.35364161832803376 1105 32-33 0.3740260305948624 1105 34-35 0.44924182044628225 1105 36-37 0.46653671071678815 1105 38-39 0.4098667097689699 1105 40-41 0.4049404130171368 1105 42-43 0.4570316145147544 1105 44-45 0.41938704663342463 1105 46-47 0.45374123531959043 1105 48-49 0.419096485617672 1105 50-51 0.43023376520626755 1105 52-53 0.5475417839034264 1105 54-55 0.6275770372443787 1105 56-57 0.5901794561188467 1105 58-59 0.6684534188916942 1105 60-61 0.56064337832197 1105 62-63 0.5997437237119456 1105 64-65 0.5785669044481523 1105 66-67 0.6106311838965439 1105 68-69 0.5760699919989349 1105 70-71 0.5743839351006841 1105 72-73 0.5992906554585602 1105 74-75 0.5769493640577537 1105 76-77 0.6022232119092834 1105 78-79 0.5745198779542733 1105 80-81 0.5686335293217155 1105 82-83 0.5771444383841668 1105 84-85 0.5734093118858432 1105 86-87 0.5660579609528291 1105 88-89 0.5880087311272959 1105 90-91 0.5333714699627023 1105 92-93 0.5563492585939045 1105 94-95 0.5191734500021923 1105 96-97 0.5131998069312793 1105 98-99 0.596738479559157 1105 100-101 0.5877932600110753 1106 1 0.26829730857434697 1106 2 0.29822996976820093 1106 3 0.3078297268383139 1106 4 0.2841048705949021 1106 5 0.31697157164605727 1106 6 0.3431924320217732 1106 7 0.3776569464502799 1106 8 0.3535162532962772 1106 9 0.3878665429463979 1106 10-11 0.4078808091599839 1106 12-13 0.35095894925972715 1106 14-15 0.4167147070821855 1106 16-17 0.463440844404829 1106 18-19 0.46418195079844793 1106 20-21 0.41147161521306685 1106 22-23 0.45052456690604004 1106 24-25 0.39381010316990483 1106 26-27 0.4339276921569706 1106 28-29 0.4766707736100031 1106 30-31 0.5069208613044722 1106 32-33 0.5482425401612616 1106 34-35 0.5543480330864128 1106 36-37 0.5850886194571814 1106 38-39 0.5342738153863351 1106 40-41 0.5126906840675645 1106 42-43 0.5572399928495244 1106 44-45 0.49027484430890667 1106 46-47 0.5300981519003543 1106 48-49 0.5091655100832071 1106 50-51 0.480734153016499 1106 52-53 0.5777231301240064 1106 54-55 0.6366453363620792 1106 56-57 0.6709767055387204 1106 58-59 0.7532641510961184 1106 60-61 0.703555324944098 1106 62-63 0.681026918314366 1106 64-65 0.724488369892228 1106 66-67 0.7346221389270653 1106 68-69 0.7915251151074756 1106 70-71 0.7774684684363748 1106 72-73 0.7634872458242938 1106 74-75 0.7008409645258027 1106 76-77 0.6620140037456324 1106 78-79 0.6919688815956917 1106 80-81 0.7075566085498082 1106 82-83 0.703942159804356 1106 84-85 0.6935640777842984 1106 86-87 0.6847984209386553 1106 88-89 0.740299484680385 1106 90-91 0.6883094735756501 1106 92-93 0.6938676688719561 1106 94-95 0.6101774334785119 1106 96-97 0.6918579847031161 1106 98-99 0.7591843054421403 1106 100-101 0.7863383746721624 1107 1 0.12611497846198105 1107 2 0.11858127639395022 1107 3 0.1194593219745066 1107 4 0.16450241410382915 1107 5 0.14579447476355512 1107 6 0.17063771326495214 1107 7 0.1581570907492491 1107 8 0.1690306287149781 1107 9 0.24223482627058246 1107 10-11 0.1979192879195537 1107 12-13 0.20395909855231054 1107 14-15 0.2806984840631799 1107 16-17 0.23713666713158688 1107 18-19 0.23168164844392436 1107 20-21 0.26083283855134454 1107 22-23 0.3174541901803707 1107 24-25 0.2539316435625665 1107 26-27 0.2803494722876039 1107 28-29 0.2971017362326691 1107 30-31 0.3254432855710334 1107 32-33 0.29424768318412475 1107 34-35 0.2929730439676348 1107 36-37 0.2926017618107082 1107 38-39 0.27725143451579726 1107 40-41 0.2508672534324603 1107 42-43 0.25855347432980125 1107 44-45 0.32352184471697143 1107 46-47 0.28081553912438295 1107 48-49 0.19493925026038994 1107 50-51 0.22301381969650436 1107 52-53 0.2760465880467322 1107 54-55 0.3607138621691064 1107 56-57 0.38291234239783734 1107 58-59 0.35879749801204497 1107 60-61 0.40152502647686106 1107 62-63 0.35365646055059585 1107 64-65 0.3640349321971428 1107 66-67 0.43668242155460035 1107 68-69 0.43125199474899034 1107 70-71 0.41826356801876585 1107 72-73 0.40419268609853987 1107 74-75 0.4140141366308896 1107 76-77 0.4443573668724028 1107 78-79 0.41715110938755373 1107 80-81 0.38374691076965917 1107 82-83 0.41893100538744577 1107 84-85 0.42980387078244675 1107 86-87 0.4022167824819718 1107 88-89 0.4477380129635975 1107 90-91 0.48388055665429164 1107 92-93 0.50858420370432 1107 94-95 0.499093320031875 1107 96-97 0.49661181754994743 1107 98-99 0.5691976006643067 1107 100-101 0.5799426070167684 1108 1 0.22952587820844172 1108 2 0.23758518652446625 1108 3 0.20582538179247223 1108 4 0.22224307425869938 1108 5 0.26194253838853143 1108 6 0.2546661319331136 1108 7 0.2324543398045762 1108 8 0.27599017598969056 1108 9 0.2984409853437455 1108 10-11 0.32718259289923424 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-0.7619514138856864 2316 98-99 -0.5939002369676487 2316 100-101 -0.6641490655634428 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 94110.0 3 19999.0 4 15515.0 5 13918.0 6 14329.0 7 15246.0 8 17855.0 9 21196.0 10 25344.0 11 30728.0 12 37310.0 13 44898.0 14 51811.0 15 62892.0 16 79289.0 17 101680.0 18 83155.0 19 50028.0 20 44226.0 21 44756.0 22 46716.0 23 50079.0 24 57972.0 25 67398.0 26 81187.0 27 99324.0 28 120667.0 29 147497.0 30 184494.0 31 235142.0 32 306334.0 33 412290.0 34 580475.0 35 874676.0 36 1384630.0 37 2185502.0 38 2524285.0 39 672586.0 40 384.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.67493552674826 21.293707891437926 15.803833215364058 21.22752336644976 2 14.961227799647927 20.971246852932627 41.39088703054553 22.676638316873916 3 17.500521814124077 27.035971916078527 31.689893320549846 23.773612949247553 4 12.024136664578148 18.190467635948796 40.35474305662151 29.430652642851552 5 12.17949870656326 38.32306816626305 35.9183382503135 13.579094876860198 6 28.071987585828882 39.66054438889173 18.501623565932668 13.765844459346718 7 25.133339981792275 34.42073864134185 23.35406374208481 17.091857634781068 8 24.68437909079599 35.89759610285566 20.92947105308259 18.488553753265755 9 24.866053544816758 17.275629021215078 22.686640074034123 35.17167735993404 10-11 22.974988760539862 27.899266911946857 29.311135781853547 19.814608545659734 12-13 24.15040943545998 25.64077038285405 29.58861573210057 20.6202044495854 14-15 21.464134802182087 27.362140835730337 27.85828028874386 23.31544407334372 16-17 19.813311976518026 30.88565335624377 28.424039702537414 20.876994964700792 18-19 19.725421397957543 28.94276329421842 31.754479153129118 19.577336154694926 20-21 20.98222139305411 27.883362407989825 31.67875303554506 19.455663163411007 22-23 20.59650500265693 27.790117954371308 31.86266675523092 19.750710287740848 24-25 20.25240610408059 28.087152130186865 31.628434543119493 20.032007222613053 26-27 19.961235064976886 28.241695099148156 31.61684449410489 20.180225341770065 28-29 20.00791572632248 28.13547580276777 31.86181681332198 19.994791657587772 30-31 20.312017769035563 28.462255478510734 31.478404309780128 19.747322442673575 32-33 19.984569398462966 28.313465711362063 31.76228634926638 19.939678540908595 34-35 20.358512175572322 28.13610651325835 31.516071500411456 19.98930981075787 36-37 20.54631044459256 28.234717202359132 31.189994637494145 20.02897771555416 38-39 20.243560318000743 28.48991208308688 31.29170805533315 19.97481954357923 40-41 20.259442274616852 28.249543573181484 31.372962238149498 20.118051914052167 42-43 20.21525540822103 28.382033852587384 31.125898082591945 20.276812656599642 44-45 20.449936421070056 28.5495361018129 30.78271677047846 20.217810706638588 46-47 20.72977337132316 28.65914822382385 30.336800339427338 20.274278065425648 48-49 20.71815348737331 28.65744709517714 30.145839001694924 20.478560415754625 50-51 20.69250652016623 28.868477517101613 29.81128447045314 20.62773149227902 52-53 20.639304131503568 29.011804597398456 29.763168492641245 20.585722778456727 54-55 20.722884778852563 29.323052987184266 29.663616825952914 20.29044540801026 56-57 20.70407892184165 28.9978580713908 29.609099749116673 20.688963257650876 58-59 20.676475719504538 28.77497459778857 29.861972257560097 20.686577425146798 60-61 20.76241672533761 28.979009106060367 29.722570394578824 20.536003774023204 62-63 20.54456531053337 28.954419337253086 29.838244584742323 20.662770767471223 64-65 20.706217700815515 28.845194747430565 29.80995455269414 20.638632999059787 66-67 20.521098001423532 28.95337633529419 29.708794532819287 20.816731130462994 68-69 20.842696946200316 29.118207881907765 29.479259650062318 20.5598355218296 70-71 20.87022359392532 28.882365245920287 29.40219749772241 20.845213662431984 72-73 20.627341764185175 29.048786678359267 29.568935340232226 20.75493621722333 74-75 20.734526972385925 29.21670355818728 29.3632344486449 20.685535020781902 76-77 20.82817230370379 29.232626919123135 29.255752919445367 20.683447857727707 78-79 20.873457689074744 29.140830071890218 29.472359341248204 20.51335289778684 80-81 20.822339445500656 29.18942825054503 29.335054062584874 20.65317824136944 82-83 20.683648527695965 29.223917123401772 29.575091949180397 20.517342399721862 84-85 20.61129426546454 29.175100756048266 29.372761572454507 20.84084340603269 86-87 20.758623771166913 29.230705782583648 29.317363018031585 20.693307428217853 88-89 20.6713977379852 29.174114587036648 29.424170208471427 20.73031746650672 90-91 20.509206292702217 29.1149270478485 29.446810482209912 20.929056177239367 92-93 20.656777095280002 29.28512567900129 29.27717601518996 20.78092121052875 94-95 20.588803168061474 29.090830523913375 29.483416123166062 20.83695018485909 96-97 20.600904537163256 29.23061403973938 29.312547352142502 20.855934070954863 98-99 20.548857423244076 29.131156493343013 29.301920944096572 21.01806513931634 100-101 20.51585346165656 29.244679388364265 29.15875611134985 21.080711038629325 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3525.0 1 2296.0 2 1502.5 3 2094.0 4 1904.5 5 1768.5 6 3023.0 7 6455.5 8 11793.0 9 15179.5 10 13530.0 11 11386.5 12 11901.0 13 12867.5 14 13722.0 15 14633.0 16 15610.5 17 16856.5 18 18489.5 19 20892.0 20 24601.5 21 29603.5 22 38162.5 23 52643.5 24 68688.0 25 86011.0 26 111391.5 27 144064.5 28 185694.0 29 223788.0 30 247043.5 31 272642.0 32 302979.0 33 330146.5 34 352505.0 35 373046.5 36 389472.0 37 400612.0 38 409097.5 39 410007.0 40 411253.5 41 404722.5 42 390786.5 43 380687.5 44 366941.0 45 354373.0 46 345156.0 47 333381.5 48 319639.5 49 308346.5 50 296724.5 51 275447.0 52 248638.0 53 227123.5 54 212138.5 55 200280.0 56 180376.5 57 156934.0 58 139825.0 59 125754.0 60 110319.0 61 92245.5 62 75752.5 63 62286.0 64 50787.0 65 41039.5 66 32775.5 67 25610.5 68 19618.0 69 14893.5 70 11207.0 71 8169.5 72 6231.0 73 4781.5 74 3640.0 75 2770.0 76 1971.5 77 1471.5 78 1121.0 79 816.0 80 653.5 81 541.5 82 449.0 83 390.5 84 323.0 85 270.5 86 221.5 87 185.0 88 172.5 89 151.0 90 123.0 91 108.0 92 97.5 93 80.5 94 69.0 95 50.0 96 35.5 97 34.0 98 29.0 99 19.5 100 13.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014724874661958622 2 0.0044954446008471805 3 0.004110120777917422 4 0.006000042385620522 5 0.002559651109461966 6 0.007027572580099878 7 0.001412854017409114 8 0.004174341415072382 9 0.010688148897932582 10-11 0.007550512054075978 12-13 0.008738593841442733 14-15 0.0033624090738989627 16-17 0.006146832413403288 18-19 0.004197277356913438 20-21 0.0010045942526382984 22-23 0.001055053324688624 24-25 0.007527576112234921 26-27 0.0011376227153164292 28-29 9.357864271151273E-4 30-31 5.779857343946375E-4 32-33 0.0014082668290409024 34-35 0.00192203192628058 36-37 0.0015412952917190333 38-39 0.0018348753472845634 40-41 0.0028578183533957075 42-43 0.0028715799185003417 44-45 0.008000056514160697 46-47 0.014376248345974554 48-49 0.005591782620849707 50-51 0.006064263022775482 52-53 0.014146888927563984 54-55 0.008610152567132814 56-57 0.005811967662523855 58-59 0.007096380405623048 60-61 0.0013669821337269996 62-63 0.0023578148212606643 64-65 0.002449558588624892 66-67 0.002431209835152046 68-69 0.002197263228373265 70-71 0.0023211173143149726 72-73 0.001417441205777325 74-75 0.0015917543637693587 76-77 0.008578042248555334 78-79 0.00673857971290256 80-81 0.013238625630658125 82-83 0.015114785673256591 84-85 0.008711070711233466 86-87 0.006055088646039059 88-89 0.0031926831042751406 90-91 0.0028715799185003417 92-93 8.348682830144763E-4 94-95 0.001233953671048869 96-97 0.0018807472309666775 98-99 0.006412889338759549 100-101 0.011435860601951042 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.0899923E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.121929752759925 #Duplication Level Percentage of deduplicated Percentage of total 1 67.64144603437734 31.87395468407031 2 17.232198992434995 16.240289408142047 3 6.666845590706419 9.424638887932954 4 3.030138538586776 5.711439014256668 5 1.5657673494641489 3.689098952530736 6 0.979365546717632 2.7689756696820953 7 0.6120425144686255 2.018843706074519 8 0.4011051721247204 1.5120679795463805 9 0.2948766029393513 1.2505639112496536 >10 1.3962029592893304 11.663253784479007 >50 0.10421060092030228 3.4138486758212503 >100 0.06886064420789004 6.123675659430053 >500 0.004797867044157561 1.544549642291726 >1k 0.002063711107455499 1.7027944087480416 >5k 1.946890275872368E-5 0.08960365623117658 >10k+ 5.8406708276171047E-5 0.9724019595133951 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 46499 0.4265993438669246 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 38759 0.35558966792701197 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20736 0.19023987600646353 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 9.174376736422817E-6 0.0 2.752313020926845E-5 0.0 4 0.0 9.174376736422817E-6 0.0 2.752313020926845E-5 0.0 5 0.0 9.174376736422817E-6 0.0 2.752313020926845E-5 0.0 6 0.0 9.174376736422817E-6 0.0 2.752313020926845E-5 0.0 7 0.0 9.174376736422817E-6 0.0 2.752313020926845E-5 0.0 8 0.0 9.174376736422817E-6 0.0 2.752313020926845E-5 0.0 9 0.0 9.174376736422817E-6 0.0 3.669750694569127E-5 0.0 10-11 0.0 1.3761565104634225E-5 0.0 4.5871883682114085E-5 0.0 12-13 9.174376736422817E-6 1.8348753472845634E-5 0.0 8.256939062780536E-5 0.0 14-15 9.174376736422817E-6 1.8348753472845634E-5 0.0 1.0091814410065099E-4 0.0 16-17 9.174376736422817E-6 1.8348753472845634E-5 0.0 1.0091814410065099E-4 0.0 18-19 9.174376736422817E-6 1.8348753472845634E-5 0.0 1.1467970920528521E-4 0.0 20-21 1.8348753472845634E-5 1.8348753472845634E-5 0.0 1.3302846267813085E-4 0.0 22-23 1.8348753472845634E-5 1.8348753472845634E-5 0.0 1.9724909983309056E-4 0.0 24-25 2.752313020926845E-5 1.8348753472845634E-5 0.0 2.9358005556553014E-4 0.0 26-27 2.752313020926845E-5 1.8348753472845634E-5 0.0 5.367010390807348E-4 0.0 28-29 2.752313020926845E-5 2.752313020926845E-5 0.0 0.0012614768012581372 0.0 30-31 2.752313020926845E-5 5.50462604185369E-5 0.0 0.0025871742396712345 0.0 32-33 2.752313020926845E-5 5.963344878674831E-5 0.0 0.005399120709384827 0.0 34-35 2.752313020926845E-5 6.422063715495973E-5 0.0 0.009871629368390951 0.0 36-37 2.752313020926845E-5 7.339501389138254E-5 0.0 0.017701959912927827 0.0 38-39 2.752313020926845E-5 7.339501389138254E-5 0.0 0.029358005556553015 0.0 40-41 2.752313020926845E-5 7.339501389138254E-5 0.0 0.041660844760096016 0.0 42-43 2.752313020926845E-5 7.339501389138254E-5 0.0 0.05463800065376609 0.0 44-45 2.752313020926845E-5 7.339501389138254E-5 0.0 0.07091793217254838 0.0 46-47 2.752313020926845E-5 7.339501389138254E-5 0.0 0.0913125716576163 0.0 48-49 2.752313020926845E-5 8.256939062780536E-5 0.0 0.11354208648996877 0.0 50-51 2.752313020926845E-5 8.256939062780536E-5 0.0 0.13662023117044036 0.0 52-53 2.752313020926845E-5 8.256939062780536E-5 0.0 0.16058829039434497 0.0 54-55 2.752313020926845E-5 8.256939062780536E-5 0.0 0.18849215723817497 0.0 56-57 2.752313020926845E-5 8.256939062780536E-5 0.0 0.22292359312996982 0.0 58-59 2.752313020926845E-5 8.256939062780536E-5 0.0 0.2626899290939945 0.0 60-61 2.752313020926845E-5 8.715657899601676E-5 0.0 0.30563059940882153 0.0 62-63 2.752313020926845E-5 9.174376736422817E-5 0.0 0.34880980351879554 0.0 64-65 2.752313020926845E-5 9.174376736422817E-5 0.0 0.3965532600551398 0.0 66-67 2.752313020926845E-5 9.174376736422817E-5 0.0 0.45298026417250836 0.0 68-69 2.752313020926845E-5 9.174376736422817E-5 0.0 0.520930285470824 0.0 70-71 2.752313020926845E-5 1.0091814410065099E-4 0.0 0.5960913668839679 0.0 72-73 2.752313020926845E-5 1.0091814410065099E-4 0.0 0.6770598287712675 0.0 74-75 2.752313020926845E-5 1.055053324688624E-4 0.0 0.7614503331812528 9.174376736422817E-6 76-77 2.752313020926845E-5 1.100925208370738E-4 0.0 0.8549601680672423 9.174376736422817E-6 78-79 2.752313020926845E-5 1.100925208370738E-4 0.0 0.9692866637681753 9.174376736422817E-6 80-81 2.752313020926845E-5 1.100925208370738E-4 0.0 1.0959297602377558 9.174376736422817E-6 82-83 2.752313020926845E-5 1.100925208370738E-4 0.0 1.230183002210199 9.174376736422817E-6 84-85 2.752313020926845E-5 1.1467970920528521E-4 0.0 1.368550952148928 9.174376736422817E-6 86-87 2.752313020926845E-5 1.1926689757349663E-4 0.0 1.5227630507114591 9.174376736422817E-6 88-89 2.752313020926845E-5 1.1926689757349663E-4 0.0 1.6974156606427404 9.174376736422817E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 22260 0.0 60.262447 1 GTATCAA 44065 0.0 44.38072 1 ATCAACG 53460 0.0 36.09194 3 TCAACGC 53930 0.0 35.761097 4 TATCAAC 55700 0.0 34.757168 2 CAACGCA 56385 0.0 34.25351 5 GTGGTAT 8530 0.0 33.468227 1 AACGCAG 59635 0.0 32.57837 6 TGGTATC 8935 0.0 30.300213 2 CGCAGAG 65545 0.0 29.171679 8 ACGCAGA 66610 0.0 28.99767 7 GCAGAGT 69700 0.0 26.642075 9 GTACATG 41075 0.0 23.799894 1 TACATGG 40490 0.0 23.073952 2 GAGTACT 45635 0.0 22.885405 12-13 CAGAGTA 67555 0.0 22.842392 10-11 AGAGTAC 65120 0.0 21.898403 10-11 ACATGGG 41265 0.0 21.76752 3 GTACTTT 48975 0.0 21.133339 14-15 AGTACTT 47435 0.0 20.514797 12-13 >>END_MODULE