FastQCFastQC Report
Wed 25 May 2016
SRR2049407_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049407_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10899923
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT453100.4156910099273178No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380940.3494887073972908No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248500.22798326190010698No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA223500.059.1176381
GTATCAA430200.042.9431531
ATCAACG555750.033.0411533
GTGGTAT87050.032.759121
TCAACGC563650.032.5190664
TATCAAC578200.031.800492
CAACGCA585100.031.3267575
TGGTATC89650.030.7302742
AACGCAG622100.029.6688986
CGCAGAG694750.026.0944678
ACGCAGA705650.025.9606257
GCAGAGT738500.024.047389
AGAGTAC695950.021.69578410-11
GAGTACT489150.021.2795412-13
GTACATG404350.020.9929681
CAGAGTA723750.020.5999210-11
AGTACTT507000.020.53971912-13
TACATGG393750.020.3991362
TATAACG11200.020.3569152
GTACTTT515950.019.9948914-15
Produced by FastQC (version 0.11.5)