##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049407_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10899923 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.04773180507789 33.0 31.0 34.0 30.0 34.0 2 32.233652659748145 34.0 31.0 34.0 30.0 34.0 3 32.29839862171504 34.0 31.0 34.0 30.0 34.0 4 35.94295574381581 37.0 35.0 37.0 35.0 37.0 5 35.82943888686186 37.0 35.0 37.0 35.0 37.0 6 35.860204792272384 37.0 35.0 37.0 35.0 37.0 7 35.788759241693725 37.0 35.0 37.0 35.0 37.0 8 35.78178845850562 37.0 35.0 37.0 33.0 37.0 9 37.37824955277207 39.0 37.0 39.0 34.0 39.0 10-11 37.37219226227562 39.0 37.0 39.0 34.0 39.0 12-13 37.35577049489248 39.0 37.0 39.0 34.0 39.0 14-15 38.56451059333172 40.0 38.0 41.0 34.0 41.0 16-17 38.56133818560002 40.0 38.0 41.0 34.0 41.0 18-19 38.61518439166956 40.0 38.0 41.0 34.0 41.0 20-21 38.61688651378546 40.0 38.0 41.0 34.0 41.0 22-23 38.540263403695604 40.0 38.0 41.0 34.0 41.0 24-25 38.44670966941693 40.0 38.0 41.0 34.0 41.0 26-27 38.27065172845717 40.0 38.0 41.0 34.0 41.0 28-29 38.091816153196675 40.0 38.0 41.0 33.0 41.0 30-31 37.905580388044946 40.0 37.0 41.0 33.0 41.0 32-33 37.657138357766385 40.0 37.0 41.0 33.0 41.0 34-35 37.477192132458185 40.0 36.5 41.0 32.5 41.0 36-37 37.28907534484418 39.5 36.0 41.0 32.0 41.0 38-39 37.43207984129796 40.0 37.0 41.0 33.0 41.0 40-41 37.337460640777 40.0 37.0 41.0 32.0 41.0 42-43 37.1453416230555 40.0 36.5 41.0 31.5 41.0 44-45 36.90229944743646 40.0 36.0 41.0 31.0 41.0 46-47 36.5647251361317 40.0 36.0 41.0 30.0 41.0 48-49 36.28482536069292 39.0 36.0 41.0 30.0 41.0 50-51 36.05885018637288 39.0 35.5 41.0 29.5 41.0 52-53 35.885561209927815 39.0 35.0 40.5 29.0 41.0 54-55 35.79185242868229 39.0 35.0 40.0 28.5 41.0 56-57 35.716135058935734 39.0 35.0 40.0 28.5 41.0 58-59 35.49440922656059 39.0 35.0 40.0 28.0 41.0 60-61 35.26982575931959 38.0 35.0 40.0 27.5 41.0 62-63 35.010960306783815 38.0 34.0 40.0 27.0 41.0 64-65 34.772463897222025 38.0 34.0 40.0 26.5 41.0 66-67 34.45145071208301 37.0 34.0 40.0 26.0 41.0 68-69 34.15054629284996 37.0 34.0 40.0 26.0 41.0 70-71 33.83149463532908 36.0 34.0 39.0 26.0 41.0 72-73 33.472473612886986 36.0 33.0 39.0 25.0 40.5 74-75 33.10273017524986 35.5 33.0 38.5 24.5 40.0 76-77 31.0847233049261 33.5 30.0 36.0 22.0 38.5 78-79 32.40718159201675 35.0 32.5 37.0 24.5 39.0 80-81 32.41244763839157 35.0 33.0 37.0 25.0 39.0 82-83 32.12866691810575 35.0 33.0 36.5 24.0 39.0 84-85 31.849265036092454 35.0 33.0 36.0 24.0 37.5 86-87 31.571526193350174 35.0 32.0 36.0 23.0 37.0 88-89 31.294184050657975 35.0 32.0 35.0 22.0 37.0 90-91 31.02285502383824 35.0 32.0 35.0 20.0 36.0 92-93 30.77483634517418 35.0 32.0 35.0 19.5 36.0 94-95 30.515811441970737 34.0 32.0 35.0 18.0 36.0 96-97 30.27491804299902 34.0 31.5 35.0 11.0 35.5 98-99 29.9943313361021 34.0 31.0 35.0 2.0 35.0 100-101 29.38923719919856 34.0 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.31522948983652554 1101 2 0.3494464689146284 1101 3 0.33553976606097535 1101 4 0.2810955155521597 1101 5 0.25669274149149146 1101 6 0.2895619458304637 1101 7 0.2853402946306147 1101 8 0.31300345418289766 1101 9 0.3509443241483936 1101 10-11 0.3352656451881515 1101 12-13 0.2693050140486619 1101 14-15 0.40388361597512557 1101 16-17 0.3758815790425487 1101 18-19 0.34024705822961465 1101 20-21 0.32061097242122827 1101 22-23 0.2617362924673401 1101 24-25 0.2412820814469896 1101 26-27 0.21999289569180291 1101 28-29 0.25086919426096443 1101 30-31 0.16921143171557418 1101 32-33 0.20939584628233376 1101 34-35 0.17539820439184695 1101 36-37 0.1683121691549374 1101 38-39 0.2263043290380864 1101 40-41 0.25659075765648964 1101 42-43 0.22169812848346027 1101 44-45 0.21499866835838333 1101 46-47 0.24543465774398499 1101 48-49 0.16873485979282776 1101 50-51 0.23023842582110632 1101 52-53 0.20253585318279477 1101 54-55 0.1738280059574251 1101 56-57 0.06610842198136169 1101 58-59 0.05669929393913975 1101 60-61 0.049758684875214954 1101 62-63 0.014727288346918499 1101 64-65 -0.06409180556764227 1101 66-67 -0.06552183908131326 1101 68-69 -0.04511841634909075 1101 70-71 -0.027614230093483627 1101 72-73 -0.07975931443643702 1101 74-75 -0.11035147369969422 1101 76-77 -0.13605886773540732 1101 78-79 -0.07459986697997323 1101 80-81 -0.043649900641817396 1101 82-83 -0.043608589699232425 1101 84-85 -0.0845125721884159 1101 86-87 -0.10396708260615384 1101 88-89 -0.21313647949616055 1101 90-91 -0.19834370763851084 1101 92-93 -0.19983235510576947 1101 94-95 -0.16551581397091653 1101 96-97 -0.32305683200796054 1101 98-99 -0.3253688900989715 1101 100-101 -0.39031954328838125 1102 1 0.3516699277053661 1102 2 0.3419024844745451 1102 3 0.32513760838973127 1102 4 0.2705434623288028 1102 5 0.2769846108019749 1102 6 0.2965405715618843 1102 7 0.3321457873112692 1102 8 0.28507411011346306 1102 9 0.42552437754451944 1102 10-11 0.35449897214068216 1102 12-13 0.2965869065557669 1102 14-15 0.39997393437720774 1102 16-17 0.42878031658553795 1102 18-19 0.3194585971389188 1102 20-21 0.32432202708266544 1102 22-23 0.24944393295476885 1102 24-25 0.2805873177402134 1102 26-27 0.2521008393295787 1102 28-29 0.3022533886383556 1102 30-31 0.2745340035311514 1102 32-33 0.2914097308388577 1102 34-35 0.3233997688439061 1102 36-37 0.26670988105937 1102 38-39 0.38606162568198243 1102 40-41 0.4069184758830673 1102 42-43 0.43693291360057884 1102 44-45 0.415854402487561 1102 46-47 0.4535581488573399 1102 48-49 0.28195186255363325 1102 50-51 0.3048969328802471 1102 52-53 0.3450421109021846 1102 54-55 0.3230876061778858 1102 56-57 0.2841307693668611 1102 58-59 0.33139781876499796 1102 60-61 0.2619786604859655 1102 62-63 0.24488909147301285 1102 64-65 0.20390566290647882 1102 66-67 0.24191910605249234 1102 68-69 0.21990618440192122 1102 70-71 0.19557901172953507 1102 72-73 0.11936807074421552 1102 74-75 0.09978914213845513 1102 76-77 0.10579673913318288 1102 78-79 0.22837150774184778 1102 80-81 0.26074340673107343 1102 82-83 0.2889068988561121 1102 84-85 0.2598120411286935 1102 86-87 0.22700209651507564 1102 88-89 0.2338537587637397 1102 90-91 0.247184611307798 1102 92-93 0.22974236081074295 1102 94-95 0.2064609561731814 1102 96-97 0.14551564636173708 1102 98-99 0.09650401869944503 1102 100-101 0.08369002365060041 1103 1 0.3150678494674253 1103 2 0.3113354987765433 1103 3 0.2739458658420304 1103 4 0.23920812167352778 1103 5 0.27424122925120287 1103 6 0.2846164466989336 1103 7 0.2795213283632023 1103 8 0.2665236463935301 1103 9 0.3833266116707108 1103 10-11 0.29676060322692166 1103 12-13 0.2884356412220015 1103 14-15 0.36494153587459977 1103 16-17 0.3867338480121205 1103 18-19 0.34888304846941054 1103 20-21 0.3082174853750814 1103 22-23 0.26252208656318743 1103 24-25 0.27685967056456917 1103 26-27 0.2596971438530673 1103 28-29 0.286964641702383 1103 30-31 0.2685520129769472 1103 32-33 0.28954271058970704 1103 34-35 0.2774744238085276 1103 36-37 0.26994121255723513 1103 38-39 0.29142492624160354 1103 40-41 0.36115930622604964 1103 42-43 0.43511399630296665 1103 44-45 0.3770641755654154 1103 46-47 0.4960104063803712 1103 48-49 0.37530154873670796 1103 50-51 0.4281565718343572 1103 52-53 0.4028652253481013 1103 54-55 0.36363254511485366 1103 56-57 0.34088660701455353 1103 58-59 0.32082137571045877 1103 60-61 0.3737383273258459 1103 62-63 0.3859682469286909 1103 64-65 0.3617624953279659 1103 66-67 0.37081944095377395 1103 68-69 0.3297524453963945 1103 70-71 0.34861223524281826 1103 72-73 0.39840518194177577 1103 74-75 0.3650115753554388 1103 76-77 0.3523911555860586 1103 78-79 0.3581010021796587 1103 80-81 0.3051269407965975 1103 82-83 0.3710629823066611 1103 84-85 0.36805827110874745 1103 86-87 0.3749915835801616 1103 88-89 0.3541324770249723 1103 90-91 0.41826387218528893 1103 92-93 0.385049005649428 1103 94-95 0.30705133531081685 1103 96-97 0.28045942253015355 1103 98-99 0.25197530859989925 1103 100-101 0.26256694470803055 1104 1 0.2998751370452979 1104 2 0.27090977761868373 1104 3 0.26674430910983205 1104 4 0.21226492068469582 1104 5 0.259495743273483 1104 6 0.28776227342060423 1104 7 0.26494258143240046 1104 8 0.26507393953355063 1104 9 0.32366075788996085 1104 10-11 0.27614885090100216 1104 12-13 0.24156574611767923 1104 14-15 0.3167880048318281 1104 16-17 0.32939553436064983 1104 18-19 0.3104493849707737 1104 20-21 0.30247365300774476 1104 22-23 0.2904853062595194 1104 24-25 0.2988471766194536 1104 26-27 0.2456356303819902 1104 28-29 0.302900351335083 1104 30-31 0.24658202433507626 1104 32-33 0.30453740734429147 1104 34-35 0.24051247355046002 1104 36-37 0.26251347449579754 1104 38-39 0.32548969682404305 1104 40-41 0.34291933645872774 1104 42-43 0.362601740377535 1104 44-45 0.3564607330492535 1104 46-47 0.3435247919555522 1104 48-49 0.3232273951680895 1104 50-51 0.39172183515928083 1104 52-53 0.3133393438401413 1104 54-55 0.28572075019594934 1104 56-57 0.25339307947943013 1104 58-59 0.3311061015284906 1104 60-61 0.326581409722408 1104 62-63 0.349502651211381 1104 64-65 0.3183660589418622 1104 66-67 0.3983143047397064 1104 68-69 0.3267760302232645 1104 70-71 0.313911388393862 1104 72-73 0.2919758111488804 1104 74-75 0.29729314010514685 1104 76-77 0.2901637298732531 1104 78-79 0.33324773922220174 1104 80-81 0.35540421284056123 1104 82-83 0.3620124511469669 1104 84-85 0.3150808537475136 1104 86-87 0.25053182560858644 1104 88-89 0.2696240294143557 1104 90-91 0.33057516851561175 1104 92-93 0.2332471340631308 1104 94-95 0.1713013019045455 1104 96-97 0.09633698437350802 1104 98-99 0.15123757287690154 1104 100-101 0.19326177425253377 1105 1 0.2941226116509128 1105 2 0.31042890242336796 1105 3 0.2905472417227202 1105 4 0.23955178937197985 1105 5 0.19392323750857798 1105 6 0.21823990710623065 1105 7 0.23448397854563297 1105 8 0.25089639214466786 1105 9 0.283880171806139 1105 10-11 0.2905536894231844 1105 12-13 0.24334309807285592 1105 14-15 0.3237203003353102 1105 16-17 0.3183852269339056 1105 18-19 0.3363033833555491 1105 20-21 0.32339796098847984 1105 22-23 0.2676595340226342 1105 24-25 0.2787708811996126 1105 26-27 0.3046381475239528 1105 28-29 0.2830143475014495 1105 30-31 0.3278476401183781 1105 32-33 0.36401317670051725 1105 34-35 0.4039612225347966 1105 36-37 0.3714099895550831 1105 38-39 0.42861263000281014 1105 40-41 0.4482024682860626 1105 42-43 0.5132008947966185 1105 44-45 0.5082111913405001 1105 46-47 0.4828036349846698 1105 48-49 0.42579225150318933 1105 50-51 0.47029296292376443 1105 52-53 0.46371133834453815 1105 54-55 0.4622001549227335 1105 56-57 0.46325460899223714 1105 58-59 0.43754546202640654 1105 60-61 0.4556755687105678 1105 62-63 0.5592904358660604 1105 64-65 0.5486538890104811 1105 66-67 0.5649900637624725 1105 68-69 0.4830963200851741 1105 70-71 0.4472400005910018 1105 72-73 0.5132564268593995 1105 74-75 0.46968244439432993 1105 76-77 0.4923459978515865 1105 78-79 0.5728280529043843 1105 80-81 0.5387372967218553 1105 82-83 0.620036544933825 1105 84-85 0.5518370805318149 1105 86-87 0.5716042629962992 1105 88-89 0.5384239839799143 1105 90-91 0.5812403504983941 1105 92-93 0.6585051088228226 1105 94-95 0.6592020440482926 1105 96-97 0.626820002122674 1105 98-99 0.6266755078076471 1105 100-101 0.7119287695124683 1106 1 0.3158178164603882 1106 2 0.25749189688719554 1106 3 0.2377702899703067 1106 4 0.24175003387211547 1106 5 0.2575225933443974 1106 6 0.2849873043160116 1106 7 0.26555510870609567 1106 8 0.27535158004534566 1106 9 0.37162400101630766 1106 10-11 0.2901510454673044 1106 12-13 0.27974200915478775 1106 14-15 0.3111984875603824 1106 16-17 0.3332053622671225 1106 18-19 0.348961928547439 1106 20-21 0.29723776247251266 1106 22-23 0.33316300235347995 1106 24-25 0.30718444631880715 1106 26-27 0.34385014628076505 1106 28-29 0.38738679494664296 1106 30-31 0.36904575443890053 1106 32-33 0.417222444000636 1106 34-35 0.3610441279307679 1106 36-37 0.43050162823032423 1106 38-39 0.4642194331973286 1106 40-41 0.4779559970350036 1106 42-43 0.46895528433179123 1106 44-45 0.4847950631448015 1106 46-47 0.5191917018997785 1106 48-49 0.44738308689149875 1106 50-51 0.5073208976959691 1106 52-53 0.49473040575512073 1106 54-55 0.5172959723767789 1106 56-57 0.49330056643071885 1106 58-59 0.5419591652629947 1106 60-61 0.5945130563313512 1106 62-63 0.584473381880315 1106 64-65 0.5389347455724902 1106 66-67 0.5378850212174555 1106 68-69 0.5699395211886298 1106 70-71 0.5379948824339849 1106 72-73 0.5159739510892294 1106 74-75 0.5134132938768303 1106 76-77 0.5035539631240589 1106 78-79 0.48093964762481534 1106 80-81 0.515403849613655 1106 82-83 0.4774154725190698 1106 84-85 0.4678051273570496 1106 86-87 0.48115586633054974 1106 88-89 0.47531031286819214 1106 90-91 0.5504544884872615 1106 92-93 0.47103224817973555 1106 94-95 0.5444542508424455 1106 96-97 0.5754900954964448 1106 98-99 0.6000191028731123 1106 100-101 0.6857786875333787 1107 1 0.2063010012002522 1107 2 0.20378543972240237 1107 3 0.17888851261418637 1107 4 0.14839328602495527 1107 5 0.16378821647079178 1107 6 0.16265963011841222 1107 7 0.17079345136908586 1107 8 0.1606795639751013 1107 9 0.21965812645080973 1107 10-11 0.18750276788285447 1107 12-13 0.15564680946195608 1107 14-15 0.19052692516688552 1107 16-17 0.20408590688024475 1107 18-19 0.12952816557753266 1107 20-21 0.1524340815632499 1107 22-23 0.16080378309243315 1107 24-25 0.1634571200882462 1107 26-27 0.2023916386948983 1107 28-29 0.22124856406944815 1107 30-31 0.25335120182661086 1107 32-33 0.21880137727846005 1107 34-35 0.23167494414748546 1107 36-37 0.21949915855613966 1107 38-39 0.2840875847226556 1107 40-41 0.30880734132217214 1107 42-43 0.35176563134456984 1107 44-45 0.23658745194261144 1107 46-47 0.2424828850489007 1107 48-49 0.2303242243797783 1107 50-51 0.26030042663634134 1107 52-53 0.22225775764313482 1107 54-55 0.29872420166311286 1107 56-57 0.38026062038996145 1107 58-59 0.42073885439809544 1107 60-61 0.40484643335064163 1107 62-63 0.4061451497524686 1107 64-65 0.5073259734320743 1107 66-67 0.4742457914588911 1107 68-69 0.5001016292771041 1107 70-71 0.4957175949634305 1107 72-73 0.4609240862740762 1107 74-75 0.4688013286021402 1107 76-77 0.4672544917563677 1107 78-79 0.5628610079385439 1107 80-81 0.5180186442951253 1107 82-83 0.51866733199126 1107 84-85 0.5070119298535438 1107 86-87 0.4871415030575825 1107 88-89 0.4782700365409731 1107 90-91 0.5884894054881293 1107 92-93 0.6411865830027104 1107 94-95 0.6947153984777223 1107 96-97 0.703882216727429 1107 98-99 0.7011730291228382 1107 100-101 0.8392823416576007 1108 1 0.11710051876556804 1108 2 0.1554213567396161 1108 3 0.11769851608985249 1108 4 0.13311451775172145 1108 5 0.12679775109427993 1108 6 0.11744980855323206 1108 7 0.12109825884460435 1108 8 0.1360585208236884 1108 9 0.20757944625623992 1108 10-11 0.15968384072561292 1108 12-13 0.10877674934959458 1108 14-15 0.17437991769147487 1108 16-17 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-1.217285683761446 2316 48-49 -0.9581853239714988 2316 50-51 -1.0932870726231414 2316 52-53 -1.0848521515976302 2316 54-55 -1.6130041052157367 2316 56-57 -1.169886468263904 2316 58-59 -0.8316263472353214 2316 60-61 -0.7836989974938646 2316 62-63 -1.3308911832724633 2316 64-65 -1.6114117754493407 2316 66-67 -1.122730825342181 2316 68-69 -1.1305072737642021 2316 70-71 -1.0024626682025968 2316 72-73 -1.1089971381229802 2316 74-75 -0.8618556892432565 2316 76-77 -0.8091458458407388 2316 78-79 -0.803558766314719 2316 80-81 -0.7679377884968304 2316 82-83 -0.807336145305662 2316 84-85 -0.9717404840273112 2316 86-87 -1.0840608315209828 2316 88-89 -0.7571338694834395 2316 90-91 -0.7195331399910145 2316 92-93 -0.7696496881637529 2316 94-95 -0.9401217587808368 2316 96-97 -0.988147764643319 2316 98-99 -1.0139620440796193 2316 100-101 -1.37219312520444 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 21.0 4 74.0 5 156.0 6 379.0 7 1212.0 8 3299.0 9 6970.0 10 11678.0 11 17670.0 12 25559.0 13 35449.0 14 45994.0 15 59194.0 16 76106.0 17 101087.0 18 85755.0 19 49184.0 20 38512.0 21 37420.0 22 38221.0 23 41272.0 24 49134.0 25 61234.0 26 78526.0 27 100081.0 28 128133.0 29 162102.0 30 206215.0 31 261483.0 32 334680.0 33 440383.0 34 612036.0 35 905930.0 36 1425033.0 37 2276042.0 38 2575164.0 39 608319.0 40 209.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.62206337005691 22.99264237353259 17.16353301918226 22.221761237228243 2 13.905411272171728 22.213490069260672 40.19626426141215 23.684834397155445 3 16.132586598311267 27.524613769215208 32.413605819138624 23.929193813334898 4 11.64726219305659 19.249943026958434 39.527666879635134 29.57512790034984 5 11.930460184290236 38.59144165572213 36.032177165240554 13.445920994747082 6 28.08429664324288 40.278039506608685 18.075283589177214 13.562380260971219 7 24.89929516015847 34.43444508736438 23.249063319071155 17.417196433405998 8 24.77186533995381 36.23771803137674 20.605991492416184 18.38442513625327 9 24.47408281352782 17.06326015535908 22.525736451034916 35.93692058007819 10-11 22.850763388455285 28.17691134959904 29.200111285194918 19.772213976750752 12-13 23.96684361898703 25.726333112628406 29.622296414387517 20.684526853997042 14-15 21.158502620379018 27.50299098029244 27.870339920592517 23.468166478736023 16-17 19.619578963998187 30.987296882739447 28.519141832469824 20.873982320792543 18-19 19.593776741888185 28.94714570087342 31.93753989422397 19.52153766301442 20-21 20.78790080094592 27.90427988904814 31.81790741937834 19.489911890627607 22-23 20.30862511597559 27.849485725724854 32.06627239476829 19.775616763531264 24-25 20.115708014330473 28.068886269077097 31.80610283627756 20.009302880314866 26-27 19.85776420028673 28.183679097083797 31.792551016555564 20.16600568607391 28-29 19.89411311510734 28.098642422548647 32.08895041495921 19.918294047384812 30-31 20.197453254854793 28.408983838686876 31.679806822054914 19.71375608440342 32-33 19.88183962632017 28.21134545107563 31.98058200762076 19.926232914983437 34-35 20.17501774702636 28.12369913631597 31.74075276018214 19.960530356475527 36-37 20.390536233841345 28.1905539228495 31.384663079443463 20.03424676386569 38-39 20.10876139527367 28.39331521010397 31.548534242806838 19.949389151815524 40-41 20.06679359066675 28.2315882407206 31.582497442475127 20.119120726137517 42-43 20.02951534367127 28.338610745774133 31.343485763830735 20.288388146723854 44-45 20.252716750713073 28.492697341238717 31.052732328935544 20.20185357911267 46-47 20.482654149190708 28.679447051334495 30.53753148723224 20.300367312242564 48-49 20.4652014105909 28.66517503368789 30.347723271882792 20.521900283838413 50-51 20.512568331228316 28.844965481568323 29.947898377362836 20.69456780984052 52-53 20.489727041627994 28.971385775954605 29.87097148871472 20.66791569370268 54-55 20.60018178263498 29.288357773620525 29.756809990907197 20.354650452837298 56-57 20.609074395177938 28.956407856952815 29.703969761190468 20.730547986678776 58-59 20.52957706679829 28.726457201626083 29.943957841544318 20.800007890031313 60-61 20.6287230608797 28.987366892803557 29.803685521764795 20.580224524551948 62-63 20.41739469017358 28.928068808021024 29.90937927923345 20.745157222571954 64-65 20.499080442349157 28.88704165927357 29.8617379061609 20.752139992216375 66-67 20.373140083206174 28.923248959349408 29.842095989274185 20.861514968170237 68-69 20.655628837084436 29.089583205455366 29.58898393375651 20.66580402370369 70-71 20.712604694460598 28.851644415383326 29.490486996649935 20.945263893506144 72-73 20.47189972566615 29.051204153301814 29.617271160780263 20.85962496025178 74-75 20.548630101418716 29.21080527426505 29.404230406972165 20.836334217344067 76-77 20.594501919201996 29.225743339560562 29.375160124636313 20.804594616601126 78-79 20.63824591871238 29.147373432800094 29.575529205994556 20.63885144249297 80-81 20.586933464919586 29.151284725167287 29.434023300289823 20.8277585096233 82-83 20.43864873697076 29.166893732845033 29.692384503132413 20.702073027051792 84-85 20.368663247786717 29.18300555254533 29.455008896439644 20.993322303228314 86-87 20.48209197364344 29.226641258476114 29.413642849622768 20.87762391825768 88-89 20.390416524326017 29.168340846518852 29.48712732114266 20.954115308012472 90-91 20.227534898989003 29.14067473939952 29.523329304352373 21.1084610572591 92-93 20.367435926558684 29.246900480132226 29.366745462395393 21.018918130913693 94-95 20.243717462644412 29.079575266708325 29.5558742849861 21.120832985661167 96-97 20.238983079925646 29.20990600549932 29.41207771489133 21.139033199683706 98-99 20.125314373101983 29.11672224991369 29.440010598294457 21.317952778689868 100-101 20.04088745042797 29.2972476911875 29.277788835451705 21.384076022932827 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3458.0 1 2341.5 2 1551.5 3 2009.5 4 1736.0 5 1600.5 6 3223.5 7 7410.5 8 12806.0 9 15692.0 10 13929.5 11 11439.0 12 11718.0 13 12993.0 14 13998.5 15 15105.0 16 16352.5 17 17581.5 18 19254.0 19 21533.5 20 25019.0 21 30337.0 22 39006.5 23 53400.5 24 69878.0 25 87124.0 26 112187.5 27 144771.5 28 186479.0 29 224978.5 30 248554.0 31 273473.0 32 303664.0 33 331338.0 34 354406.0 35 375950.0 36 392545.5 37 403140.5 38 410775.5 39 412795.0 40 413929.5 41 407288.0 42 394585.5 43 382749.0 44 369372.5 45 357932.0 46 347436.5 47 335129.5 48 320966.0 49 310357.0 50 298429.0 51 275836.0 52 247281.5 53 224962.0 54 209957.0 55 196661.0 56 176685.5 57 153592.5 58 136389.0 59 122857.5 60 107555.0 61 89243.0 62 72803.5 63 59222.5 64 47999.0 65 38612.5 66 30519.5 67 23476.5 68 17623.0 69 13158.5 70 9640.5 71 6663.5 72 4801.0 73 3573.5 74 2721.0 75 1969.5 76 1300.5 77 875.5 78 623.5 79 423.5 80 272.0 81 189.5 82 134.0 83 86.5 84 56.5 85 38.5 86 31.5 87 31.0 88 22.0 89 9.5 90 5.5 91 3.0 92 4.5 93 4.0 94 2.0 95 2.0 96 1.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04933062371174549 2 0.0021192810261136705 3 2.568825486198389E-4 4 2.844056788291073E-4 5 0.002458732965361315 6 0.0016238646823468386 7 0.0 8 9.174376736422817E-6 9 1.1926689757349663E-4 10-11 4.5871883682114085E-6 12-13 0.0 14-15 6.28444806444963E-4 16-17 0.0 18-19 5.045907205032549E-5 20-21 5.596369809217918E-4 22-23 0.0 24-25 6.697295017588656E-4 26-27 0.007692714893490532 28-29 0.0077018892702269545 30-31 0.0011743202222621206 32-33 0.006908305682526382 34-35 0.008385380337090454 36-37 0.004578013991474986 38-39 0.0026009358047758687 40-41 0.0020596475773269223 42-43 0.0015458824800872449 44-45 8.394554713826878E-4 46-47 6.28444806444963E-4 48-49 0.0021605657214275736 50-51 0.001527533726614399 52-53 0.0021926760400050533 54-55 8.440426597508991E-4 56-57 2.1101066493772478E-4 58-59 8.53217036487322E-4 60-61 0.00573398546026426 62-63 0.0024174482700474124 64-65 0.0010734020781614697 66-67 0.005610131374322552 68-69 0.007889963993323623 70-71 0.0026559820651944057 72-73 5.963344878674831E-4 74-75 0.0032752524949029456 76-77 0.001931206303017003 78-79 0.0024633201537295263 80-81 9.311992387469159E-4 82-83 1.559644045191879E-4 84-85 5.229394739761006E-4 86-87 0.005050494393400761 88-89 0.0028211208464500163 90-91 0.001325697438413097 92-93 8.256939062780535E-5 94-95 4.357828949800838E-4 96-97 2.752313020926845E-4 98-99 5.137650972396778E-4 100-101 1.3761565104634224E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.0899923E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.76557093514514 #Duplication Level Percentage of deduplicated Percentage of total 1 65.59446230311032 30.019680174856994 2 18.46655538164425 16.902649004928524 3 6.985172802695996 9.59041264188091 4 3.217542159449564 5.890106157404365 5 1.7138018862279714 3.921656089647589 6 0.9885605643655948 2.714522317929645 7 0.6340781039066131 2.0313262509932306 8 0.4357337527061117 1.5953283174646842 9 0.31524293675953047 1.298454568566459 >10 1.4589361515272838 11.935295109799071 >50 0.10917029839903448 3.4616037836904763 >100 0.07346650738428104 6.31105057361596 >500 0.005022318149381156 1.5387608454398463 >1k 0.0021541718812754505 1.6458459442329547 >5k 4.026471708680483E-5 0.14588954893578102 >10k+ 6.039707563020725E-5 0.9974186706134924 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 45310 0.4156910099273178 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 38094 0.3494887073972908 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24850 0.22798326190010698 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 9.174376736422817E-6 0.0 0.0 0.0 4 0.0 9.174376736422817E-6 0.0 2.752313020926845E-5 0.0 5 0.0 9.174376736422817E-6 0.0 2.752313020926845E-5 0.0 6 0.0 9.174376736422817E-6 0.0 3.669750694569127E-5 0.0 7 0.0 9.174376736422817E-6 0.0 3.669750694569127E-5 0.0 8 0.0 9.174376736422817E-6 0.0 3.669750694569127E-5 0.0 9 0.0 9.174376736422817E-6 0.0 4.5871883682114085E-5 0.0 10-11 0.0 9.174376736422817E-6 0.0 4.5871883682114085E-5 0.0 12-13 0.0 9.174376736422817E-6 0.0 6.422063715495973E-5 0.0 14-15 0.0 9.174376736422817E-6 0.0 6.880782552317114E-5 0.0 16-17 0.0 9.174376736422817E-6 0.0 7.798220225959395E-5 0.0 18-19 0.0 9.174376736422817E-6 0.0 8.715657899601676E-5 0.0 20-21 0.0 9.174376736422817E-6 0.0 1.055053324688624E-4 0.0 22-23 0.0 1.8348753472845634E-5 0.0 1.7431315799203351E-4 0.0 24-25 0.0 2.2935941841057042E-5 0.0 2.614697369880503E-4 0.0 26-27 0.0 2.752313020926845E-5 0.0 4.862419670304093E-4 0.0 28-29 0.0 3.2110318577479856E-5 0.0 0.0011926689757349662 0.0 30-31 0.0 3.669750694569127E-5 0.0 0.0024770817188341605 0.0 32-33 0.0 3.669750694569127E-5 0.0 0.0052523306816020625 0.0 34-35 0.0 3.669750694569127E-5 0.0 0.009683554645294284 0.0 36-37 0.0 4.5871883682114085E-5 0.0 0.01748636205962189 0.0 38-39 0.0 5.50462604185369E-5 0.0 0.029078187066092116 0.0 40-41 0.0 5.50462604185369E-5 0.0 0.041335154385953005 0.0 42-43 0.0 5.50462604185369E-5 0.0 0.0542847871494138 0.0 44-45 0.0 5.50462604185369E-5 0.0 0.07042251582878153 0.0 46-47 0.0 5.50462604185369E-5 0.0 0.09061990621401637 0.0 48-49 0.0 5.50462604185369E-5 0.0 0.11270721820695431 0.0 50-51 0.0 6.422063715495973E-5 0.0 0.13563398567127494 0.0 52-53 0.0 6.422063715495973E-5 0.0 0.15928094170940474 0.0 54-55 0.0 6.422063715495973E-5 0.0 0.1870059082068745 0.0 56-57 0.0 6.422063715495973E-5 0.0 0.22118504873841768 0.0 58-59 0.0 6.422063715495973E-5 0.0 0.26064863027014046 0.0 60-61 0.0 6.422063715495973E-5 0.0 0.30334617960145227 0.0 62-63 0.0 6.422063715495973E-5 0.0 0.34630061148138386 0.0 64-65 0.0 6.422063715495973E-5 0.0 0.393800947034213 0.0 66-67 0.0 6.422063715495973E-5 0.0 0.4497095988659736 0.0 68-69 0.0 6.422063715495973E-5 0.0 0.5171687910088906 0.0 70-71 0.0 6.422063715495973E-5 0.0 0.5916509685435393 0.0 72-73 0.0 6.422063715495973E-5 0.0 0.6719175906105025 0.0 74-75 0.0 6.880782552317114E-5 0.0 0.7557805683581434 0.0 76-77 0.0 7.339501389138254E-5 0.0 0.8482995705565994 0.0 78-79 0.0 7.339501389138254E-5 0.0 0.9618554186116728 0.0 80-81 0.0 7.339501389138254E-5 0.0 1.0876774083633434 0.0 82-83 0.0 7.339501389138254E-5 0.0 1.220696696664738 0.0 84-85 0.0 7.339501389138254E-5 0.0 1.3583215220878166 0.0 86-87 0.0 8.256939062780536E-5 0.0 1.5112767310374577 0.0 88-89 0.0 8.256939062780536E-5 0.0 1.685021077671833 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 22350 0.0 59.117638 1 GTATCAA 43020 0.0 42.943153 1 ATCAACG 55575 0.0 33.041153 3 GTGGTAT 8705 0.0 32.75912 1 TCAACGC 56365 0.0 32.519066 4 TATCAAC 57820 0.0 31.80049 2 CAACGCA 58510 0.0 31.326757 5 TGGTATC 8965 0.0 30.730274 2 AACGCAG 62210 0.0 29.668898 6 CGCAGAG 69475 0.0 26.094467 8 ACGCAGA 70565 0.0 25.960625 7 GCAGAGT 73850 0.0 24.04738 9 AGAGTAC 69595 0.0 21.695784 10-11 GAGTACT 48915 0.0 21.27954 12-13 GTACATG 40435 0.0 20.992968 1 CAGAGTA 72375 0.0 20.59992 10-11 AGTACTT 50700 0.0 20.539719 12-13 TACATGG 39375 0.0 20.399136 2 TATAACG 1120 0.0 20.356915 2 GTACTTT 51595 0.0 19.99489 14-15 >>END_MODULE