FastQCFastQC Report
Wed 25 May 2016
SRR2049406_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049406_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10904028
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT482380.4423869784633715No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT405540.37191760696139076No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214940.19711981664023606No Hit
GATCAGCACCATGAAGCTTCTCACGGGCCTGGTTTTCTGCTCCTTGGTCC141690.12994280645647646No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA233000.061.3867951
GTATCAA453000.044.189231
GTGGTAT86250.035.634061
ATCAACG564400.035.0368923
TGGTATC85200.034.733032
TCAACGC571300.034.6239434
TATCAAC588200.033.836332
CAACGCA597350.033.0817575
AACGCAG636100.031.1937226
CGCAGAG688450.028.1330188
ACGCAGA700900.027.9586327
GTACATG450400.023.4877781
TACATGG437200.022.68532
GAGTACT461250.022.56935912-13
GCAGAGT839500.022.3241189
GTACTTT474200.021.7205214-15
CAGAGTA744400.021.37797210-11
ACATGGG454300.021.26743
AGAGTAC687600.021.08515410-11
AGTACTT462500.020.82909412-13
Produced by FastQC (version 0.11.5)