##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049406_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10904028 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.505485037272464 31.0 31.0 34.0 30.0 34.0 2 31.53990672070908 33.0 31.0 34.0 30.0 34.0 3 31.556559649333256 33.0 31.0 34.0 28.0 34.0 4 35.184377186118745 37.0 35.0 37.0 33.0 37.0 5 35.208138405367265 37.0 35.0 37.0 33.0 37.0 6 35.3021930978167 37.0 35.0 37.0 33.0 37.0 7 35.24915755902314 37.0 35.0 37.0 33.0 37.0 8 35.23072437084718 37.0 35.0 37.0 33.0 37.0 9 36.80832633591917 39.0 37.0 39.0 33.0 39.0 10-11 36.745958557699964 39.0 37.0 39.0 33.0 39.0 12-13 36.707679079694216 39.0 37.0 39.0 33.0 39.0 14-15 37.86672947831755 40.0 38.0 41.0 33.0 41.0 16-17 37.85673454800373 40.0 38.0 41.0 33.0 41.0 18-19 37.89767171360896 40.0 38.0 41.0 33.0 41.0 20-21 37.86567821542644 40.0 38.0 41.0 33.0 41.0 22-23 37.76515389542286 40.0 38.0 41.0 32.5 41.0 24-25 37.65962981753165 40.0 38.0 41.0 32.0 41.0 26-27 37.52329189726952 40.0 37.0 41.0 32.0 41.0 28-29 37.367752082074624 40.0 37.0 41.0 32.0 41.0 30-31 37.177031735428415 40.0 37.0 41.0 31.5 41.0 32-33 36.92540352977817 40.0 36.0 41.0 31.0 41.0 34-35 36.72907681454963 39.5 36.0 41.0 30.5 41.0 36-37 36.55061271852934 39.0 36.0 41.0 30.0 41.0 38-39 36.348350398586646 39.0 35.0 41.0 30.0 41.0 40-41 36.12903768222165 39.0 35.0 40.0 30.0 41.0 42-43 35.89439420918582 39.0 35.0 40.0 29.0 41.0 44-45 35.60148098482506 39.0 35.0 40.0 27.5 41.0 46-47 35.21554699786171 38.0 35.0 40.0 26.5 41.0 48-49 34.881593893559334 38.0 34.0 40.0 25.0 41.0 50-51 33.51254994943153 36.5 32.5 39.0 23.0 40.0 52-53 33.8123461806958 37.5 33.5 39.0 23.5 40.0 54-55 34.84936383142083 38.5 35.0 40.0 26.0 41.0 56-57 34.94667158778389 39.0 35.0 40.0 26.0 41.0 58-59 34.77705912897509 38.5 34.5 40.0 26.0 41.0 60-61 34.50729945851202 38.0 34.0 40.0 25.0 41.0 62-63 34.22804742430962 38.0 34.0 40.0 24.5 41.0 64-65 33.936376309745356 37.0 34.0 40.0 23.5 41.0 66-67 33.6072570613355 37.0 33.5 40.0 23.0 41.0 68-69 33.25372958506709 36.0 33.0 39.0 22.0 41.0 70-71 32.91975350760288 36.0 33.0 39.0 21.5 41.0 72-73 32.670148178269535 35.0 33.0 39.0 20.5 40.0 74-75 32.47512400921934 35.0 33.0 38.0 20.0 40.0 76-77 32.14443767936032 35.0 33.0 37.0 19.5 39.5 78-79 31.781947964550348 35.0 32.0 37.0 18.0 39.0 80-81 31.45848318621339 35.0 32.0 36.5 17.5 39.0 82-83 31.129564826869483 35.0 32.0 36.0 14.0 38.0 84-85 30.815562056517095 35.0 32.0 36.0 9.0 37.0 86-87 30.531842957483235 35.0 31.0 35.0 7.0 37.0 88-89 30.255132965542643 35.0 31.0 35.0 2.0 36.5 90-91 29.989745532568328 34.5 31.0 35.0 2.0 36.0 92-93 29.74948097161893 34.0 31.0 35.0 2.0 36.0 94-95 29.488708484607706 34.0 31.0 35.0 2.0 36.0 96-97 29.237304737295247 34.0 31.0 35.0 2.0 35.0 98-99 28.92158351941136 34.0 30.0 35.0 2.0 35.0 100-101 28.38747190487772 34.0 29.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.25302937438901907 1101 2 0.2991060364717697 1101 3 0.2870279988367841 1101 4 0.293338092349849 1101 5 0.2664872292278204 1101 6 0.2759580653699203 1101 7 0.2538333146465348 1101 8 0.2866268369157652 1101 9 0.2825785633480393 1101 10-11 0.3086830812105248 1101 12-13 0.28460222709856 1101 14-15 0.3605886998411947 1101 16-17 0.41860622018332805 1101 18-19 0.3767674504567893 1101 20-21 0.2789198854957746 1101 22-23 0.3106124926844842 1101 24-25 0.27969172668172604 1101 26-27 0.3088216095405443 1101 28-29 0.2687763379154262 1101 30-31 0.2497551948104828 1101 32-33 0.24582780242273827 1101 34-35 0.23810898171638684 1101 36-37 0.2702021835432973 1101 38-39 0.16209696537767115 1101 40-41 0.11902688991074939 1101 42-43 0.10975484228846 1101 44-45 0.0624004646866112 1101 46-47 -0.02929501888613828 1101 48-49 -0.1408307935197115 1101 50-51 -0.17969389752029485 1101 52-53 -0.07365888207163351 1101 54-55 0.18343332045369465 1101 56-57 0.19984824890405406 1101 58-59 0.20499156170814103 1101 60-61 0.17284948516502396 1101 62-63 0.10458828058336422 1101 64-65 0.1276605197925491 1101 66-67 0.10885286866154331 1101 68-69 0.09979662523713273 1101 70-71 0.03482963657153704 1101 72-73 0.03355194425872554 1101 74-75 0.00851237402891769 1101 76-77 -0.01713061367171065 1101 78-79 -0.07689602147823038 1101 80-81 -0.13687120948367593 1101 82-83 -0.17272003035389716 1101 84-85 -0.20715888221486978 1101 86-87 -0.21856039439180464 1101 88-89 -0.22396426834914962 1101 90-91 -0.304398653812882 1101 92-93 -0.3008997715588677 1101 94-95 -0.4874071704833902 1101 96-97 -0.5732837358519625 1101 98-99 -0.7042042588992885 1101 100-101 -0.8198411712083882 1102 1 0.23540332934543784 1102 2 0.26938446766856927 1102 3 0.25458886057797514 1102 4 0.28517861121885346 1102 5 0.308131377826669 1102 6 0.320243696058391 1102 7 0.3621459174824153 1102 8 0.3617947459669608 1102 9 0.37552007871699544 1102 10-11 0.38063584810929996 1102 12-13 0.32791837258440637 1102 14-15 0.4530488893426181 1102 16-17 0.4043083816958415 1102 18-19 0.3710012612610356 1102 20-21 0.3879931451936969 1102 22-23 0.3384043830373642 1102 24-25 0.34287054236339287 1102 26-27 0.38908356080703754 1102 28-29 0.3684214762917577 1102 30-31 0.2867284255164648 1102 32-33 0.2802375982574645 1102 34-35 0.2591354660166516 1102 36-37 0.2522754399112017 1102 38-39 0.18498714354108614 1102 40-41 0.2346004032447908 1102 42-43 0.0573997145758085 1102 44-45 0.11320234435500964 1102 46-47 0.08365527107142157 1102 48-49 -0.01924014316715983 1102 50-51 -0.06665895668250954 1102 52-53 0.07774812543082277 1102 54-55 0.25123009899058246 1102 56-57 0.24286840465022408 1102 58-59 0.2937346743879985 1102 60-61 0.30762957342567887 1102 62-63 0.2877853639969743 1102 64-65 0.2048295551865138 1102 66-67 0.17817833204869515 1102 68-69 0.17006213268176396 1102 70-71 0.1297256450702875 1102 72-73 0.15477460197671888 1102 74-75 0.13869183892470005 1102 76-77 0.11656437110422502 1102 78-79 0.09492595736077547 1102 80-81 0.009111472983260427 1102 82-83 -0.04205499468909579 1102 84-85 -0.06144718836198493 1102 86-87 -0.1047034125547448 1102 88-89 -0.12076622547506943 1102 90-91 -0.21050256459595929 1102 92-93 -0.3048131414705644 1102 94-95 -0.35899427835942177 1102 96-97 -0.40633915749391036 1102 98-99 -0.4144337595198877 1102 100-101 -0.5136902733982787 1103 1 0.2711796245413254 1103 2 0.26140240996999964 1103 3 0.2764589361727161 1103 4 0.2592540515196049 1103 5 0.24411340744547516 1103 6 0.26550705751383674 1103 7 0.24745185989616658 1103 8 0.23761069714560534 1103 9 0.3026822206414863 1103 10-11 0.33830467435505085 1103 12-13 0.27938562388960975 1103 14-15 0.3830098818687233 1103 16-17 0.4034328696579834 1103 18-19 0.35090505797958116 1103 20-21 0.31419430729484077 1103 22-23 0.26120811918384135 1103 24-25 0.37309665467309827 1103 26-27 0.3701198997003843 1103 28-29 0.33579228764319424 1103 30-31 0.3541642381816459 1103 32-33 0.25884143555794736 1103 34-35 0.3487711410909782 1103 36-37 0.3386079985995778 1103 38-39 0.2798797601673826 1103 40-41 0.2028059608320376 1103 42-43 0.26989246063644856 1103 44-45 0.22702064754827944 1103 46-47 0.21033353846967628 1103 48-49 0.26146965145609613 1103 50-51 0.13672768864798712 1103 52-53 0.25782299143351395 1103 54-55 0.3774106609067971 1103 56-57 0.44368714709124646 1103 58-59 0.4165220223143393 1103 60-61 0.4423029689846629 1103 62-63 0.4091575669682115 1103 64-65 0.3983113307502961 1103 66-67 0.377412882662135 1103 68-69 0.3839675082185323 1103 70-71 0.3563480872209581 1103 72-73 0.4051698664580172 1103 74-75 0.3809969135399882 1103 76-77 0.3177838101239985 1103 78-79 0.3699032679075991 1103 80-81 0.33985244784585333 1103 82-83 0.31442766204446215 1103 84-85 0.30241702859030184 1103 86-87 0.17021751282060293 1103 88-89 0.1540547743785119 1103 90-91 0.19564022669316117 1103 92-93 0.2274929209507519 1103 94-95 0.10498740561084219 1103 96-97 0.03390242208648786 1103 98-99 -0.0016038055640166249 1103 100-101 -0.015503123099996685 1104 1 -0.0476023628475879 1104 2 0.025530260425654205 1104 3 0.04891315156695697 1104 4 0.011060415049499284 1104 5 -0.009651368486167655 1104 6 -0.05891953437192399 1104 7 -0.060552471019185816 1104 8 -0.05192876635488375 1104 9 -0.12336305904571532 1104 10-11 -0.018316474566027807 1104 12-13 -0.030814468003114825 1104 14-15 -0.01655698564605501 1104 16-17 -0.020994786938437926 1104 18-19 -0.08423895232681389 1104 20-21 -0.046730852555086244 1104 22-23 -0.06301625957796375 1104 24-25 -0.055488942740147706 1104 26-27 -0.08574935874501932 1104 28-29 -0.07932304099612253 1104 30-31 -0.08975315173751142 1104 32-33 -0.12980010830526112 1104 34-35 -0.11886763370551279 1104 36-37 -0.17314464618264225 1104 38-39 -0.16158359453933002 1104 40-41 -0.10913906897576453 1104 42-43 -0.1623258885870058 1104 44-45 -0.08946914064801348 1104 46-47 -0.07822229618567178 1104 48-49 -0.08850459073608619 1104 50-51 -0.18178517264724547 1104 52-53 -0.052082748913043986 1104 54-55 0.05395049869402868 1104 56-57 0.028982796765411933 1104 58-59 0.02885681812330887 1104 60-61 0.016277017890921286 1104 62-63 0.006197917045078327 1104 64-65 0.015209681185886836 1104 66-67 0.05767875729327443 1104 68-69 0.05225804973856896 1104 70-71 0.032611141187864234 1104 72-73 -0.003955072423501349 1104 74-75 0.00619865573423084 1104 76-77 0.005203967062357151 1104 78-79 0.01652559620048777 1104 80-81 0.02036631815918355 1104 82-83 -0.023449378616835048 1104 84-85 -0.03908203487382522 1104 86-87 -0.02740105704366158 1104 88-89 -0.09143937012238013 1104 90-91 -0.08785024701298738 1104 92-93 -0.12765364466122264 1104 94-95 -0.11788466776573969 1104 96-97 -0.18736349431407007 1104 98-99 -0.17824196528535907 1104 100-101 -0.23558712877080978 1105 1 0.2933610565548328 1105 2 0.29857701820555604 1105 3 0.2936904768990267 1105 4 0.2999147961676627 1105 5 0.28880298126875203 1105 6 0.31164365804779237 1105 7 0.3092374633496888 1105 8 0.3413675072468223 1105 9 0.4043708250579314 1105 10-11 0.3568897456195401 1105 12-13 0.2977642676650589 1105 14-15 0.41359609379300366 1105 16-17 0.40708720759752737 1105 18-19 0.38380555141989703 1105 20-21 0.4339326533608343 1105 22-23 0.3459447028761744 1105 24-25 0.3781685950987921 1105 26-27 0.41205365438785435 1105 28-29 0.4041318423028386 1105 30-31 0.4094564935523053 1105 32-33 0.3524053960253397 1105 34-35 0.4306989128662977 1105 36-37 0.46237508063639154 1105 38-39 0.4147399815254076 1105 40-41 0.43760480911739563 1105 42-43 0.46919943727773017 1105 44-45 0.4275274120401207 1105 46-47 0.4360893714645684 1105 48-49 0.3974367561375374 1105 50-51 0.3506393453011256 1105 52-53 0.4465284130849625 1105 54-55 0.6096972250545676 1105 56-57 0.6101533418950993 1105 58-59 0.6170778852643153 1105 60-61 0.6240106446427447 1105 62-63 0.5812100851363837 1105 64-65 0.5719073786567108 1105 66-67 0.5642772620711582 1105 68-69 0.5500223879431161 1105 70-71 0.558881755690301 1105 72-73 0.5580994834373669 1105 74-75 0.5646185780684974 1105 76-77 0.5829777567331149 1105 78-79 0.5824588288753141 1105 80-81 0.5319222769081691 1105 82-83 0.5251388943014881 1105 84-85 0.48173404780329676 1105 86-87 0.5085517904901344 1105 88-89 0.49295373294494027 1105 90-91 0.4751332928124441 1105 92-93 0.4678552413124386 1105 94-95 0.45512749182734424 1105 96-97 0.4851574720428289 1105 98-99 0.44605129730000925 1105 100-101 0.39442822334781624 1106 1 0.30829644219517505 1106 2 0.32812896861935315 1106 3 0.31361361125022924 1106 4 0.3100685651145696 1106 5 0.33282610533157 1106 6 0.323741783300612 1106 7 0.38484072660437363 1106 8 0.389751701119188 1106 9 0.4109988936480491 1106 10-11 0.46024686854221386 1106 12-13 0.43646789426638577 1106 14-15 0.47465490892763995 1106 16-17 0.5267880919766696 1106 18-19 0.49586123658490067 1106 20-21 0.5190801026882426 1106 22-23 0.5479657917223406 1106 24-25 0.5452246082986179 1106 26-27 0.5595191345618957 1106 28-29 0.5485238996837651 1106 30-31 0.5892878298081499 1106 32-33 0.6403481295891567 1106 34-35 0.5837617974950362 1106 36-37 0.6108273493261507 1106 38-39 0.6308991852357835 1106 40-41 0.5518482432815048 1106 42-43 0.6235081134115035 1106 44-45 0.6186087478607689 1106 46-47 0.6519150548824015 1106 48-49 0.576524834213501 1106 50-51 0.5354077656266796 1106 52-53 0.6491748529856807 1106 54-55 0.6687985147200664 1106 56-57 0.7066446671247917 1106 58-59 0.7201708840813055 1106 60-61 0.7038817410029878 1106 62-63 0.7084071057850636 1106 64-65 0.7410439212774733 1106 66-67 0.7464519037652693 1106 68-69 0.7453981259496203 1106 70-71 0.7159643401565177 1106 72-73 0.7257568897735425 1106 74-75 0.7303465218434511 1106 76-77 0.7179316128839304 1106 78-79 0.7575484931452117 1106 80-81 0.7280339011160031 1106 82-83 0.7167361997992039 1106 84-85 0.7378678962796421 1106 86-87 0.7574308226537134 1106 88-89 0.785825113893516 1106 90-91 0.7720939802976616 1106 92-93 0.6933915045769083 1106 94-95 0.7131702252883336 1106 96-97 0.6981311227778519 1106 98-99 0.7583282843220971 1106 100-101 0.8127935724026614 1107 1 0.13167613495037855 1107 2 0.11921159747127774 1107 3 0.12268096876318424 1107 4 0.13502084218029609 1107 5 0.1188955535635472 1107 6 0.13328637015635536 1107 7 0.1031890585741877 1107 8 0.1764620001829087 1107 9 0.21109888455796977 1107 10-11 0.1896532861406115 1107 12-13 0.1927127810948548 1107 14-15 0.25102523889764683 1107 16-17 0.23877336604266475 1107 18-19 0.207064763536998 1107 20-21 0.24597083989394974 1107 22-23 0.28475562900985807 1107 24-25 0.2386365708474827 1107 26-27 0.31139623664353167 1107 28-29 0.3158336897028491 1107 30-31 0.30755435103348816 1107 32-33 0.32199525257797745 1107 34-35 0.37635792511979815 1107 36-37 0.368363027900827 1107 38-39 0.36986382481399716 1107 40-41 0.35434347098807706 1107 42-43 0.3055120221470773 1107 44-45 0.41966319745626635 1107 46-47 0.3987585236579676 1107 48-49 0.30675163177735243 1107 50-51 0.3620803408471147 1107 52-53 0.40004295588541794 1107 54-55 0.45936300885696113 1107 56-57 0.39152785956184033 1107 58-59 0.406813007524633 1107 60-61 0.4958165651098909 1107 62-63 0.49524693852753643 1107 64-65 0.4955889626373491 1107 66-67 0.5173954543515791 1107 68-69 0.5136578296129315 1107 70-71 0.5554857473013257 1107 72-73 0.5356332646485527 1107 74-75 0.5802556210162422 1107 76-77 0.5380956888770214 1107 78-79 0.5254514110617627 1107 80-81 0.5223548719368374 1107 82-83 0.5229083659659146 1107 84-85 0.5432401351670144 1107 86-87 0.5661732097094365 1107 88-89 0.5601683554170123 1107 90-91 0.5487370127492568 1107 92-93 0.5009203655895433 1107 94-95 0.5158040767563783 1107 96-97 0.5331461214143403 1107 98-99 0.6147027048293232 1107 100-101 0.632500871739083 1108 1 0.19106376734605135 1108 2 0.1564920741128546 1108 3 0.16488566426860984 1108 4 0.218217090644508 1108 5 0.26539661908311984 1108 6 0.24775618113122988 1108 7 0.2559106641966622 1108 8 0.2876478003353782 1108 9 0.2818335284945448 1108 10-11 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-0.6760848563489823 2316 88-89 -0.6914857232944733 2316 90-91 -0.6381616556914267 2316 92-93 -0.803626814084673 2316 94-95 -1.0788730668956639 2316 96-97 -0.784159596281242 2316 98-99 -0.710908615815427 2316 100-101 -0.6771783983644184 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 91557.0 3 20500.0 4 16324.0 5 14745.0 6 15091.0 7 16183.0 8 18405.0 9 21913.0 10 25935.0 11 31219.0 12 37214.0 13 44069.0 14 50624.0 15 61821.0 16 79268.0 17 101980.0 18 82885.0 19 49812.0 20 44390.0 21 45651.0 22 47639.0 23 52612.0 24 59935.0 25 70940.0 26 85384.0 27 104343.0 28 127822.0 29 157340.0 30 196359.0 31 251850.0 32 328563.0 33 442996.0 34 629017.0 35 962696.0 36 1541386.0 37 2251183.0 38 2197592.0 39 526504.0 40 281.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.48804260470437 20.35397889173249 15.65191996153528 21.506058542027862 2 15.51454802134235 20.515089586639395 40.35321796632096 23.617144425697287 3 18.03755158610714 26.080031813305204 31.167922855669687 24.71449374491797 4 12.390151096182416 17.825802003157552 39.01647616621112 30.767570734448913 5 12.823508501492883 37.98876384709382 35.142628230407716 14.045099421005578 6 29.367362223551496 38.23659756589025 18.265324896181912 14.13071531437634 7 26.174736692086086 33.86013716782548 22.366418745493 17.598707394595444 8 25.90422766379541 34.87027709321712 20.05496906660166 19.170526176385806 9 25.642606736692343 16.81249081662808 21.763440374991585 35.78146207168799 10-11 23.855269526720633 27.2307730757828 28.311282915410636 20.60267448208593 12-13 24.934464195413202 24.94088902701589 28.529989816114533 21.594656961456376 14-15 22.212131299107174 26.607246746120573 26.886382926213116 24.294239028559137 16-17 20.79332712026299 29.84107144601363 27.458865082717406 21.906736351005975 18-19 20.868555978911584 27.977918332589695 30.770817751727293 20.38270793677143 20-21 21.923675966034633 26.92926982021438 30.74350433802268 20.403549875728306 22-23 21.626711416375798 26.94260415097084 30.85206981314129 20.57861461951207 24-25 21.013108145606196 27.025443815718024 30.782517905083147 21.178930133592633 26-27 20.98147294309591 27.37064912679319 30.588336714763294 21.0595412153476 28-29 20.82345884906793 27.088223574128445 31.19937783985521 20.888939736948416 30-31 21.197084165197133 27.400640967881586 30.485230688963664 20.917044177957614 32-33 20.788054443619462 27.3302938103637 30.722404562622707 21.159247183394132 34-35 21.330883496290234 27.316546639539258 30.468532939429814 20.884036924740695 36-37 21.617638791408467 27.128305205321507 30.174284446325117 21.079771556944905 38-39 21.23869899636766 27.680817319140722 30.27320595714713 20.80727772734449 40-41 21.11387372067411 27.127502713403718 30.588998335020907 21.169625230901268 42-43 21.082104258579687 27.49973644423447 30.031201609315755 21.386957687870094 44-45 21.142842704139618 27.733751044645143 29.84674532963828 21.276660921576955 46-47 21.789376319329083 27.74544158602772 29.28911135858615 21.176070736057046 48-49 21.53673696753586 27.908167956276397 29.180737272942594 21.374357803245154 50-51 21.486746155782384 28.07942901226005 28.839759152665977 21.59406567929159 52-53 21.62511211798825 28.002760264843808 28.80519440495582 21.56693321221212 54-55 21.55443278156027 28.4552616609836 28.789380577399896 21.20092498005623 56-57 21.759014879030065 28.019346201299456 28.840752671234494 21.380886248435985 58-59 21.48829227072181 27.8919016038143 28.933917505713985 21.685888619749903 60-61 21.75845019242412 27.908873226513226 28.818019703668647 21.51465687739401 62-63 21.747930720640777 27.92481168943366 29.01760542842144 21.309652161504122 64-65 21.79447379153683 28.12684253645087 28.680425433842387 21.398258238169912 66-67 21.626775310520134 28.027291315937163 28.78595152691115 21.55998184663155 68-69 21.79096396083422 28.385616615999332 28.618090530607482 21.205328892558963 70-71 21.857328100778197 27.810956872139926 28.719797076006714 21.611917951075164 72-73 21.32685681091815 28.168525436364632 28.68406130395399 21.820556448763227 74-75 21.603610422403445 28.018196097507158 28.741466395001815 21.636727085087585 76-77 21.722603530780336 28.38410625606561 28.369239020888465 21.52405119226559 78-79 21.71679802475178 28.142554686346756 28.628199518890472 21.512447770010994 80-81 21.681010186643928 28.191894664957175 28.5664959605953 21.560599187803597 82-83 21.671316471250744 28.1562747092288 28.791463407307518 21.380945412212938 84-85 21.359898264230424 28.096834248718938 28.83877603589019 21.70449145116045 86-87 21.6940610366842 28.167590252477055 28.578479434882237 21.55986927595651 88-89 21.566726945865927 28.245644187398305 28.638987514887138 21.54864135184863 90-91 21.502048352387686 28.112637439357517 28.721968336895788 21.663345871359006 92-93 21.734808098715632 28.23724096085937 28.38832873644187 21.639622203983127 94-95 21.50561237495352 28.03795671005835 28.83148962680356 21.624941288184576 96-97 21.671529318198264 28.302732087786538 28.582820999427817 21.442917594587385 98-99 21.46028614619783 28.284491659323734 28.344060548405224 21.91116164607321 100-101 21.50661184354894 28.333601536079446 28.189441604634176 21.97034501573744 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4720.0 1 3062.5 2 1845.5 3 2486.0 4 2129.5 5 1928.0 6 4023.0 7 8447.5 8 14455.0 9 18242.5 10 15619.0 11 12106.5 12 12105.0 13 13061.5 14 13745.5 15 14386.0 16 15115.5 17 15568.0 18 16735.0 19 18690.0 20 21363.0 21 25265.5 22 32640.5 23 45187.5 24 57665.5 25 70151.0 26 90186.5 27 117595.5 28 154671.5 29 185614.5 30 198574.0 31 217732.0 32 246202.5 33 269071.0 34 288930.5 35 310182.5 36 322665.5 37 331959.0 38 347197.5 39 355126.0 40 364636.0 41 367959.5 42 361258.0 43 361370.5 44 357770.0 45 351079.5 46 347156.0 47 341617.5 48 332973.5 49 327375.0 50 334194.5 51 327374.0 52 297441.5 53 275674.0 54 271744.5 55 278464.5 56 282076.0 57 262502.5 58 226269.5 59 197161.5 60 164717.5 61 127958.0 62 98804.5 63 76601.0 64 60103.5 65 48802.0 66 40331.5 67 32462.5 68 24618.5 69 17686.5 70 13249.5 71 9862.0 72 7362.0 73 5288.0 74 3794.0 75 2798.5 76 1988.0 77 1475.5 78 1159.5 79 913.0 80 697.5 81 506.0 82 401.5 83 340.0 84 299.0 85 263.5 86 233.0 87 199.0 88 164.0 89 148.5 90 125.5 91 98.0 92 84.5 93 72.5 94 63.0 95 56.5 96 44.5 97 27.0 98 17.5 99 13.5 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014398348940409911 2 0.0041177443784993955 3 0.0037967620772800656 4 0.005887732496651696 5 0.002714593176026327 6 0.0075384986172082466 7 0.0014581767398249529 8 0.004044376995363548 9 0.010729979783617576 10-11 0.0072954691605707535 12-13 0.00934058496548248 14-15 0.003572074466426535 16-17 0.006139932876181169 18-19 0.003787591154388085 20-21 8.89579520522141E-4 22-23 0.0011096816699296811 24-25 0.007057025165379253 26-27 0.0010133869795638823 28-29 7.611866000344093E-4 30-31 4.539606831530513E-4 32-33 0.0011371944386056236 34-35 0.0015911551217586749 36-37 0.0011830490530655277 38-39 0.001692035273570464 40-41 0.0026366403314444903 42-43 0.00241653818203695 44-45 0.007373422005152592 46-47 0.013476671189765838 48-49 0.004911029208655737 50-51 0.0055621647339863765 52-53 0.01330700911626419 54-55 0.008015386607591251 56-57 0.0054612845821745875 58-59 0.006781897478619827 60-61 0.0010959252855917098 62-63 0.0021276541109395536 64-65 0.0022101924169673813 66-67 0.0024440509507128922 68-69 0.002054286727803707 70-71 0.0022101924169673813 72-73 0.0012013908988494893 74-75 0.0013893948181350965 76-77 0.007864066379873566 78-79 0.006323351334020786 80-81 0.012559578900567754 82-83 0.014659720242831365 84-85 0.008084168529281106 86-87 0.005516310119526472 88-89 0.0029530371712178285 90-91 0.002425709104928931 92-93 9.308486735360548E-4 94-95 0.0012151472831874606 96-97 0.001549885968744761 98-99 0.006286667642452862 100-101 0.011064718469174877 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.0904028E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.09194277308251 #Duplication Level Percentage of deduplicated Percentage of total 1 64.52345939297975 23.933004633257177 2 16.96106351642844 12.582375946439623 3 7.1728701577681 7.981670682320571 4 3.536672422403866 5.247282043957731 5 2.0659142120976237 3.8314385864611444 6 1.2902137130621578 2.871391992596871 7 0.8576784806692658 2.2269072785882127 8 0.604664443277817 1.7942543141582865 9 0.4525385324514861 1.510698001347474 >10 2.226425096113921 14.607016460514528 >50 0.16851584067500586 4.3702494870519635 >100 0.11830515471537364 8.693583319962976 >500 0.013563480336605586 3.4659848047774298 >1k 0.007842556977260533 5.289447084507086 >5k 1.489091146735417E-4 0.3514954112743124 >10k+ 1.240909288946181E-4 1.243199952784868 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 48238 0.4423869784633715 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 40554 0.37191760696139076 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21494 0.19711981664023606 No Hit GATCAGCACCATGAAGCTTCTCACGGGCCTGGTTTTCTGCTCCTTGGTCC 14169 0.12994280645647646 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.834184578396167E-5 0.0 10-11 0.0 0.0 0.0 1.834184578396167E-5 0.0 12-13 0.0 0.0 0.0 1.834184578396167E-5 0.0 14-15 0.0 0.0 0.0 2.75127686759425E-5 0.0 16-17 0.0 0.0 0.0 5.044007590589459E-5 0.0 18-19 0.0 0.0 0.0 5.502553735188501E-5 0.0 20-21 0.0 4.585461445990417E-6 0.0 7.336738313584667E-5 0.0 22-23 0.0 9.170922891980834E-6 0.0 1.329783819337221E-4 0.0 24-25 0.0 9.170922891980834E-6 0.0 2.2010214940754004E-4 0.0 26-27 0.0 9.170922891980834E-6 0.0 4.7230252893701297E-4 0.0 28-29 0.0 9.170922891980834E-6 0.0 0.001196805437403499 0.0 30-31 0.0 9.170922891980834E-6 0.0 0.0029576226326638194 0.0 32-33 0.0 1.375638433797125E-5 0.0 0.005988612648463485 0.0 34-35 0.0 1.834184578396167E-5 0.0 0.010610757786021826 0.0 36-37 0.0 1.834184578396167E-5 0.0 0.018630729855059064 0.0 38-39 0.0 1.834184578396167E-5 0.0 0.03087849737729947 0.0 40-41 0.0 1.834184578396167E-5 0.0 0.04399291711283207 0.0 42-43 0.0 3.668369156792334E-5 0.0 0.05658917970496774 0.0 44-45 0.0 3.668369156792334E-5 0.0 0.07276210222497595 0.0 46-47 0.0 3.668369156792334E-5 0.0 0.0936534645729083 0.0 48-49 0.0 3.668369156792334E-5 0.0 0.11674584841491603 0.0 50-51 0.0 6.419646024386584E-5 0.0 0.14079200823768978 0.0 52-53 0.0 6.419646024386584E-5 0.0 0.16508119751710104 0.0 54-55 0.0 7.336738313584667E-5 0.0 0.19288285026414093 0.0 56-57 0.0 8.253830602782751E-5 0.0 0.22717293095725727 0.0 58-59 0.0 8.253830602782751E-5 0.0 0.2661631096325138 0.0 60-61 0.0 9.170922891980835E-5 0.0 0.30882624292600863 0.0 62-63 0.0 9.170922891980835E-5 0.0 0.3514251797592596 0.0 64-65 0.0 9.170922891980835E-5 0.0 0.39824274112282176 0.0 66-67 0.0 9.170922891980835E-5 0.0 0.4537864356181037 0.0 68-69 0.0 9.170922891980835E-5 0.0 0.5198812769006096 0.0 70-71 0.0 9.170922891980835E-5 0.0 0.5926846482785995 0.0 72-73 0.0 1.1005107470377002E-4 0.0 0.6734713080340586 0.0 74-75 0.0 1.1005107470377002E-4 0.0 0.7582748320162054 0.0 76-77 0.0 1.1005107470377002E-4 0.0 0.8497502024022683 0.0 78-79 0.0 1.1005107470377002E-4 0.0 0.9584623223638091 0.0 80-81 4.585461445990417E-6 1.1005107470377002E-4 0.0 1.0817057696476935 0.0 82-83 9.170922891980834E-6 1.1005107470377002E-4 0.0 1.213028799999413 0.0 84-85 9.170922891980834E-6 1.1463653614976043E-4 0.0 1.346575779152438 9.170922891980834E-6 86-87 9.170922891980834E-6 1.1922199759575086E-4 0.0 1.4922834020602296 9.170922891980834E-6 88-89 9.170922891980834E-6 1.1922199759575086E-4 0.0 1.6611109215787048 9.170922891980834E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 23300 0.0 61.386795 1 GTATCAA 45300 0.0 44.18923 1 GTGGTAT 8625 0.0 35.63406 1 ATCAACG 56440 0.0 35.036892 3 TGGTATC 8520 0.0 34.73303 2 TCAACGC 57130 0.0 34.623943 4 TATCAAC 58820 0.0 33.83633 2 CAACGCA 59735 0.0 33.081757 5 AACGCAG 63610 0.0 31.193722 6 CGCAGAG 68845 0.0 28.133018 8 ACGCAGA 70090 0.0 27.958632 7 GTACATG 45040 0.0 23.487778 1 TACATGG 43720 0.0 22.6853 2 GAGTACT 46125 0.0 22.569359 12-13 GCAGAGT 83950 0.0 22.324118 9 GTACTTT 47420 0.0 21.72052 14-15 CAGAGTA 74440 0.0 21.377972 10-11 ACATGGG 45430 0.0 21.2674 3 AGAGTAC 68760 0.0 21.085154 10-11 AGTACTT 46250 0.0 20.829094 12-13 >>END_MODULE