FastQCFastQC Report
Wed 25 May 2016
SRR2049406_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049406_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10904028
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT476250.4367652027305873No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400650.36743302566721214No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270750.2483027373003811No Hit
GATCAGCACCATGAAGCTTCTCACGGGCCTGGTTTTCTGCTCCTTGGTCC139770.12818198926121613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA228200.062.095221
GTATCAA441800.043.5715181
GTGGTAT77950.035.783941
ATCAACG572850.033.2655683
TGGTATC83000.033.0199662
TCAACGC582250.032.712054
TATCAAC596800.032.010482
CAACGCA606400.031.4014595
AACGCAG651400.029.3701886
CGCAGAG716950.026.0880648
ACGCAGA738150.025.5833117
GTACATG429100.021.849161
TACATGG415200.021.1752222
GAGTACT504100.021.13370112-13
AGAGTAC728200.021.07727410-11
AGTACTT508850.020.81974812-13
GCAGAGT883350.020.754759
GTACTTT509250.020.69166214-15
CAGAGTA792350.019.78443710-11
ACATGGG431850.019.6547073
Produced by FastQC (version 0.11.5)