##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049406_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10904028 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01511762442283 33.0 31.0 34.0 30.0 34.0 2 32.21109575287224 33.0 31.0 34.0 30.0 34.0 3 32.27472224025837 34.0 31.0 34.0 30.0 34.0 4 35.931105367667804 37.0 35.0 37.0 35.0 37.0 5 35.81362281901697 37.0 35.0 37.0 35.0 37.0 6 35.840969043733196 37.0 35.0 37.0 35.0 37.0 7 35.76593677125554 37.0 35.0 37.0 33.0 37.0 8 35.759674681686434 37.0 35.0 37.0 33.0 37.0 9 37.35112547399915 39.0 37.0 39.0 34.0 39.0 10-11 37.3412782413985 39.0 37.0 39.0 34.0 39.0 12-13 37.32142213868123 39.0 37.0 39.0 34.0 39.0 14-15 38.516440438340766 40.0 38.0 41.0 34.0 41.0 16-17 38.51086768119084 40.0 38.0 41.0 34.0 41.0 18-19 38.556494627489954 40.0 38.0 41.0 34.0 41.0 20-21 38.55176000098312 40.0 38.0 41.0 34.0 41.0 22-23 38.46908179252658 40.0 38.0 41.0 34.0 41.0 24-25 38.37145525488379 40.0 38.0 41.0 34.0 41.0 26-27 38.19341682724953 40.0 38.0 41.0 33.5 41.0 28-29 38.00369478141472 40.0 37.5 41.0 33.0 41.0 30-31 37.80837260322515 40.0 37.0 41.0 33.0 41.0 32-33 37.556439051697225 40.0 36.5 41.0 32.5 41.0 34-35 37.37255494024777 39.0 36.0 41.0 32.0 41.0 36-37 37.18381432072625 39.0 36.0 41.0 31.5 41.0 38-39 37.33956662620456 39.5 36.0 41.0 32.0 41.0 40-41 37.24009480716667 40.0 36.0 41.0 32.0 41.0 42-43 37.042613839582955 40.0 36.0 41.0 31.0 41.0 44-45 36.788579046202 39.5 36.0 41.0 31.0 41.0 46-47 36.427817454247176 39.0 36.0 41.0 30.0 41.0 48-49 36.121413206202334 39.0 35.0 41.0 29.5 41.0 50-51 35.879107381235634 39.0 35.0 40.0 29.0 41.0 52-53 35.6866028773954 39.0 35.0 40.0 28.0 41.0 54-55 35.56919103656007 39.0 35.0 40.0 28.0 41.0 56-57 35.460455118053616 38.5 35.0 40.0 28.0 41.0 58-59 35.20987574499992 38.0 35.0 40.0 27.5 41.0 60-61 34.95140130784697 38.0 34.0 40.0 27.0 41.0 62-63 34.66303608171219 37.5 34.0 40.0 26.0 41.0 64-65 34.394004536672135 37.0 34.0 40.0 26.0 41.0 66-67 34.04738290290524 36.5 34.0 39.5 26.0 41.0 68-69 33.71985558914559 36.0 33.0 39.0 26.0 41.0 70-71 33.38905122950895 35.5 33.0 39.0 25.0 40.5 72-73 33.03350115205133 35.0 33.0 38.5 24.5 40.0 74-75 32.66731427138669 35.0 32.5 37.5 24.0 40.0 76-77 30.69286524209219 33.5 30.0 35.5 21.0 38.5 78-79 32.0196951071659 35.0 32.0 36.0 23.5 39.0 80-81 32.05229503262464 35.0 32.5 36.0 24.0 39.0 82-83 31.80089302778753 35.0 32.0 36.0 23.0 37.5 84-85 31.54447677500461 35.0 32.0 35.5 23.0 37.0 86-87 31.28946995550635 35.0 32.0 35.0 21.5 37.0 88-89 31.036288287227435 35.0 32.0 35.0 20.0 36.0 90-91 30.770344225088195 34.5 32.0 35.0 19.5 36.0 92-93 30.53719066018539 34.0 31.5 35.0 18.0 36.0 94-95 30.279675409857717 34.0 31.0 35.0 15.0 35.5 96-97 30.03715044568851 34.0 31.0 35.0 4.5 35.0 98-99 29.75155011524182 34.0 31.0 35.0 2.0 35.0 100-101 29.1384215997978 34.0 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3633465081397773 1101 2 0.38920923048465284 1101 3 0.38152075804532615 1101 4 0.31172090913175765 1101 5 0.3215431577635002 1101 6 0.32040513113658164 1101 7 0.3407786592620923 1101 8 0.3503837500443936 1101 9 0.36753586009703554 1101 10-11 0.3491366369394697 1101 12-13 0.35076695097451704 1101 14-15 0.45425504305699604 1101 16-17 0.4594259217992587 1101 18-19 0.39154279979074147 1101 20-21 0.3476783099061862 1101 22-23 0.30206570472454075 1101 24-25 0.27663298065078123 1101 26-27 0.24738623443092678 1101 28-29 0.24778855727088 1101 30-31 0.20025833373006918 1101 32-33 0.2665536731809226 1101 34-35 0.23182095340717268 1101 36-37 0.17495760972775543 1101 38-39 0.3109395459751525 1101 40-41 0.35180177758040543 1101 42-43 0.36876655298476635 1101 44-45 0.3761249256568533 1101 46-47 0.4369878549774171 1101 48-49 0.26005752227897005 1101 50-51 0.29813252844707705 1101 52-53 0.2655226126215311 1101 54-55 0.21946429945535328 1101 56-57 0.1959781712095534 1101 58-59 0.224726522254457 1101 60-61 0.21741479730409452 1101 62-63 0.18585746528545855 1101 64-65 0.08223546656957836 1101 66-67 0.09722245608212887 1101 68-69 0.08471031943205531 1101 70-71 0.050462563622026835 1101 72-73 0.03884340800486541 1101 74-75 0.04506042838114155 1101 76-77 0.035566051215003114 1101 78-79 0.034669195763797234 1101 80-81 0.05283032793168019 1101 82-83 0.04650423487651523 1101 84-85 0.027282537444325072 1101 86-87 0.011214371820290836 1101 88-89 -0.05054553280398011 1101 90-91 -0.020802592431266476 1101 92-93 -0.03120755650586915 1101 94-95 -0.09010291855356556 1101 96-97 -0.2144428413267292 1101 98-99 -0.2510507244992084 1101 100-101 -0.26721939642871106 1102 1 0.3577729394789344 1102 2 0.34231749044030124 1102 3 0.3275146671710303 1102 4 0.26294949722428385 1102 5 0.28770856992808547 1102 6 0.2850811508893969 1102 7 0.3112119708277632 1102 8 0.31205580173787695 1102 9 0.3889996603055934 1102 10-11 0.334844829767583 1102 12-13 0.2965187055973928 1102 14-15 0.3762210320217534 1102 16-17 0.38307215030444297 1102 18-19 0.30930682624604344 1102 20-21 0.30176268907802495 1102 22-23 0.2858559045053042 1102 24-25 0.3085682113802122 1102 26-27 0.23544975789445033 1102 28-29 0.2783975532985181 1102 30-31 0.21585462210961737 1102 32-33 0.19025430493326922 1102 34-35 0.17134610528532335 1102 36-37 0.15198265060542582 1102 38-39 0.27388384103914376 1102 40-41 0.25284594790065995 1102 42-43 0.3530946238427646 1102 44-45 0.34345494974188284 1102 46-47 0.34906995721705414 1102 48-49 0.13214264511939433 1102 50-51 0.1654475570598848 1102 52-53 0.19607225200523004 1102 54-55 0.2179357706995333 1102 56-57 0.23883598874154188 1102 58-59 0.24939043618994816 1102 60-61 0.299585705484418 1102 62-63 0.2482568971086394 1102 64-65 0.17796085250613203 1102 66-67 0.1896998289794496 1102 68-69 0.17024275599475658 1102 70-71 0.14722850108948649 1102 72-73 0.1166762913584023 1102 74-75 0.06952379565311162 1102 76-77 0.07961402812356866 1102 78-79 0.06813413029328075 1102 80-81 0.10897493860475294 1102 82-83 0.2237382159624488 1102 84-85 0.19615626093003868 1102 86-87 0.06508934336774175 1102 88-89 0.041559294704381244 1102 90-91 -0.002182256177484021 1102 92-93 0.039022786330932036 1102 94-95 0.0010519572365410568 1102 96-97 -0.07709441856388821 1102 98-99 -0.07571699372631358 1102 100-101 -0.1616050637057178 1103 1 0.31678341857990233 1103 2 0.3388704318291431 1103 3 0.2998703722773399 1103 4 0.25029217532520676 1103 5 0.22303487017164514 1103 6 0.26902711338686913 1103 7 0.25859224572867134 1103 8 0.25641140121078365 1103 9 0.3475266827339709 1103 10-11 0.3192697026899296 1103 12-13 0.32361407450868285 1103 14-15 0.35808142009747 1103 16-17 0.3746167730628258 1103 18-19 0.3236893405082242 1103 20-21 0.3128021652165742 1103 22-23 0.30566036236860583 1103 24-25 0.3562182191690013 1103 26-27 0.23559106764167126 1103 28-29 0.23370873225420752 1103 30-31 0.2515818947153363 1103 32-33 0.24771936066826328 1103 34-35 0.20349826385042036 1103 36-37 0.21872776790196014 1103 38-39 0.29394428440526355 1103 40-41 0.3408323629770891 1103 42-43 0.44129108559177155 1103 44-45 0.4273560514715271 1103 46-47 0.4267850448760555 1103 48-49 0.40258052733012306 1103 50-51 0.39229789659104597 1103 52-53 0.34655463897247074 1103 54-55 0.3545352375851323 1103 56-57 0.3778134273187277 1103 58-59 0.2972073932259036 1103 60-61 0.300750907100479 1103 62-63 0.30611450963328934 1103 64-65 0.2532603783592364 1103 66-67 0.2341121479067496 1103 68-69 0.2795645611358992 1103 70-71 0.2146845295142299 1103 72-73 0.2955194585152441 1103 74-75 0.2138047413929769 1103 76-77 0.1772637852001715 1103 78-79 0.3166525466078909 1103 80-81 0.23172825652986973 1103 82-83 0.3538678960068893 1103 84-85 0.31583798681937836 1103 86-87 0.2517108394977505 1103 88-89 0.24003669450474874 1103 90-91 0.2669646922540245 1103 92-93 0.24492624776370775 1103 94-95 0.2185052194701278 1103 96-97 0.2562415277475978 1103 98-99 0.18714421568254735 1103 100-101 0.1673134090358488 1104 1 0.29289991102987045 1104 2 0.3012009687954844 1104 3 0.2717514457350063 1104 4 0.22615780556429854 1104 5 0.21035236443244543 1104 6 0.24122530681858478 1104 7 0.25468230059402686 1104 8 0.2606252802874991 1104 9 0.3187971036796142 1104 10-11 0.27407650275119266 1104 12-13 0.2607681356836764 1104 14-15 0.30624639128067344 1104 16-17 0.312768713825605 1104 18-19 0.3091207817415196 1104 20-21 0.2670800088335099 1104 22-23 0.2843765096885207 1104 24-25 0.29411606263852264 1104 26-27 0.2989747262987166 1104 28-29 0.34388802133871366 1104 30-31 0.3194352906099738 1104 32-33 0.3197323353961252 1104 34-35 0.29243485541677217 1104 36-37 0.3494521642724422 1104 38-39 0.4029682805264443 1104 40-41 0.40872785589308336 1104 42-43 0.3799349226779398 1104 44-45 0.40025965666371377 1104 46-47 0.4949734151234537 1104 48-49 0.42128679741118447 1104 50-51 0.4522805827577514 1104 52-53 0.4235258074269055 1104 54-55 0.4220494002843864 1104 56-57 0.348519346864272 1104 58-59 0.37253497602967656 1104 60-61 0.38675045336993463 1104 62-63 0.4231980787727352 1104 64-65 0.4543946228118969 1104 66-67 0.4792925713176075 1104 68-69 0.4755375387794274 1104 70-71 0.4123084229317797 1104 72-73 0.347705555195887 1104 74-75 0.447156352259249 1104 76-77 0.3647033526328016 1104 78-79 0.5038573453407196 1104 80-81 0.5045389861924647 1104 82-83 0.4671523727712241 1104 84-85 0.4151782347659214 1104 86-87 0.3857988206343528 1104 88-89 0.38023333018287175 1104 90-91 0.36856335530504225 1104 92-93 0.2829393666370166 1104 94-95 0.2761778111150939 1104 96-97 0.20221090175506262 1104 98-99 0.23677971608426773 1104 100-101 0.2904101773114043 1105 1 0.3259859754927348 1105 2 0.3072188084880665 1105 3 0.29140261455723504 1105 4 0.20970142313083073 1105 5 0.2442620268870641 1105 6 0.2523343913620266 1105 7 0.25878882597000086 1105 8 0.2708130212491753 1105 9 0.33877397889452965 1105 10-11 0.31248334022838264 1105 12-13 0.27698707698638003 1105 14-15 0.34381873540383623 1105 16-17 0.29235209168774645 1105 18-19 0.3112590867807228 1105 20-21 0.2856057204052931 1105 22-23 0.24517420253342692 1105 24-25 0.2690891068388126 1105 26-27 0.26504618335980723 1105 28-29 0.2743613113861585 1105 30-31 0.2665808860816057 1105 32-33 0.38894713253273494 1105 34-35 0.3905846561454851 1105 36-37 0.34252975296121235 1105 38-39 0.40487835217847845 1105 40-41 0.3672345522917695 1105 42-43 0.3940084396760213 1105 44-45 0.3839868638905344 1105 46-47 0.37625897086349624 1105 48-49 0.36822318829797496 1105 50-51 0.335439884258399 1105 52-53 0.3543423935836003 1105 54-55 0.3167646966113793 1105 56-57 0.2732983710390755 1105 58-59 0.3437754126234367 1105 60-61 0.39830132521137074 1105 62-63 0.37127345191083094 1105 64-65 0.3641682678525342 1105 66-67 0.41192101453068375 1105 68-69 0.35892909027982256 1105 70-71 0.37603777395541726 1105 72-73 0.43941968830866784 1105 74-75 0.41329898594009506 1105 76-77 0.44879966731459575 1105 78-79 0.5017148513193419 1105 80-81 0.38868970953949855 1105 82-83 0.5093538949631693 1105 84-85 0.4782955494367336 1105 86-87 0.5218680056235705 1105 88-89 0.5167795580665526 1105 90-91 0.5457694276536174 1105 92-93 0.5826383131059956 1105 94-95 0.5348378483132556 1105 96-97 0.5053549593691606 1105 98-99 0.5128469464823802 1105 100-101 0.6332499961645794 1106 1 0.28744987052541404 1106 2 0.2578690190201982 1106 3 0.23684321261553265 1106 4 0.20502094140065452 1106 5 0.2576272337246621 1106 6 0.24500455575520164 1106 7 0.2621996190016276 1106 8 0.24239447711445905 1106 9 0.35991491876082193 1106 10-11 0.2925592059437889 1106 12-13 0.28182968961654353 1106 14-15 0.37928800823211617 1106 16-17 0.3485900093448464 1106 18-19 0.32616644594797606 1106 20-21 0.3116589322289869 1106 22-23 0.2840156636775575 1106 24-25 0.3258474918490677 1106 26-27 0.29669507100839354 1106 28-29 0.3964530364985279 1106 30-31 0.359917397178954 1106 32-33 0.4147558464737102 1106 34-35 0.4533929255897533 1106 36-37 0.436071137050547 1106 38-39 0.4078769742864665 1106 40-41 0.4234589485823861 1106 42-43 0.41779599675916046 1106 44-45 0.4397204953027938 1106 46-47 0.5025484608745572 1106 48-49 0.5055322235900164 1106 50-51 0.5217789165562223 1106 52-53 0.5249483808268636 1106 54-55 0.49545758238975424 1106 56-57 0.43137633859016944 1106 58-59 0.5095837559008487 1106 60-61 0.515241259908997 1106 62-63 0.5500205810393268 1106 64-65 0.47726851096427225 1106 66-67 0.5266200256338109 1106 68-69 0.5483108551075233 1106 70-71 0.6002734275042343 1106 72-73 0.5201680986959403 1106 74-75 0.46594424413815716 1106 76-77 0.45027267040450525 1106 78-79 0.5210266174901932 1106 80-81 0.5445067912631316 1106 82-83 0.49306546585309263 1106 84-85 0.4692398530902828 1106 86-87 0.49769407751755423 1106 88-89 0.49201222009804724 1106 90-91 0.518856847975826 1106 92-93 0.506939991406341 1106 94-95 0.5712520662908176 1106 96-97 0.5393392137136104 1106 98-99 0.6188655398834477 1106 100-101 0.7161587079866685 1107 1 0.17521461918462933 1107 2 0.18912981349343028 1107 3 0.20529153549527734 1107 4 0.16740617910631528 1107 5 0.18813809637351397 1107 6 0.16054405216462442 1107 7 0.1581681673154165 1107 8 0.13622685598910778 1107 9 0.169554951485118 1107 10-11 0.14299098487297357 1107 12-13 0.1360429429571539 1107 14-15 0.17676778279805916 1107 16-17 0.18560709870035907 1107 18-19 0.15124371165109096 1107 20-21 0.10423915220899005 1107 22-23 0.16157679448384243 1107 24-25 0.17772913897205456 1107 26-27 0.21934073958397704 1107 28-29 0.18451775788749103 1107 30-31 0.17592503284844696 1107 32-33 0.2267729358292243 1107 34-35 0.1268794268169131 1107 36-37 0.1873664738381109 1107 38-39 0.20363872321838272 1107 40-41 0.1994702202849581 1107 42-43 0.2173392201024953 1107 44-45 0.19074540212945124 1107 46-47 0.23270253777666028 1107 48-49 0.13418870936403238 1107 50-51 0.17047903472730042 1107 52-53 0.16030198504466142 1107 54-55 0.20364547439956482 1107 56-57 0.23680539049453841 1107 58-59 0.26608316504547247 1107 60-61 0.2591190891972488 1107 62-63 0.24823892482624643 1107 64-65 0.26919651750913687 1107 66-67 0.29262766130329254 1107 68-69 0.3595575599525347 1107 70-71 0.33657921788692136 1107 72-73 0.38945270918589614 1107 74-75 0.39182825468265037 1107 76-77 0.324588641679842 1107 78-79 0.4271510607226219 1107 80-81 0.4366484147519003 1107 82-83 0.4320619207208338 1107 84-85 0.4241644054037117 1107 86-87 0.4388857873621106 1107 88-89 0.405206475165766 1107 90-91 0.47492675071194057 1107 92-93 0.5003610440379163 1107 94-95 0.5151935261581038 1107 96-97 0.4926798191131212 1107 98-99 0.5695677487735509 1107 100-101 0.6613455091865568 1108 1 0.11745441933501155 1108 2 0.15479217557140146 1108 3 0.1391790702661737 1108 4 0.10488796116039367 1108 5 0.10125718770689929 1108 6 0.11425397249713853 1108 7 0.16964828117170327 1108 8 0.1364771378629257 1108 9 0.22996807655028562 1108 10-11 0.12420621863321202 1108 12-13 0.07718330470727608 1108 14-15 0.15791477990034508 1108 16-17 0.1875246871343137 1108 18-19 0.1266486568193841 1108 20-21 0.154646099841095 1108 22-23 0.12471168924673037 1108 24-25 0.17626602778616984 1108 26-27 0.17848398562779266 1108 28-29 0.1824184150983541 1108 30-31 0.2029556989596486 1108 32-33 0.2461002541639914 1108 34-35 0.29770644138873337 1108 36-37 0.28827140849067234 1108 38-39 0.24665258936668977 1108 40-41 0.21522735073417465 1108 42-43 0.2930170224070352 1108 44-45 0.24243283028127394 1108 46-47 0.17921536077928835 1108 48-49 0.132700718404287 1108 50-51 0.24009282844384217 1108 52-53 0.2855164537667747 1108 54-55 0.2208125519255688 1108 56-57 0.14186630268430633 1108 58-59 0.24293628682315926 1108 60-61 0.2676881576421337 1108 62-63 0.2923638899043297 1108 64-65 0.2952064715069085 1108 66-67 0.32957750394766805 1108 68-69 0.3583555558778002 1108 70-71 0.32817825199085604 1108 72-73 0.3436737015398421 1108 74-75 0.35508596722610264 1108 76-77 0.21948653541605978 1108 78-79 0.39856008748643035 1108 80-81 0.3995112578388387 1108 82-83 0.4105774752780178 1108 84-85 0.4432476804530445 1108 86-87 0.44719626575929183 1108 88-89 0.5118598532289944 1108 90-91 0.5378637446237065 1108 92-93 0.5083959394123525 1108 94-95 0.6029204888373982 1108 96-97 0.6033130436645635 1108 98-99 0.6044847252068273 1108 100-101 0.7210467490435413 1109 1 0.09843520889952373 1109 2 0.08914198370593596 1109 3 0.05935394803560001 1109 4 0.12648096176697265 1109 5 0.08975183579652679 1109 6 0.06858045647753386 1109 7 0.10460124059283515 1109 8 0.10075861694856059 1109 9 0.12185534132734688 1109 10-11 0.07794190201052231 1109 12-13 0.06376405089463333 1109 14-15 0.07269503436426561 1109 16-17 0.0987843962893038 1109 18-19 0.032821579702257964 1109 20-21 0.006298345574336395 1109 22-23 0.08308992974587426 1109 24-25 0.07750109027890062 1109 26-27 0.10301032446550096 1109 28-29 0.07510249485932263 1109 30-31 0.15405089920720627 1109 32-33 0.17195337790231235 1109 34-35 0.08810402039991772 1109 36-37 0.1821036258062776 1109 38-39 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2316 54-55 -2.0011356377026743 2316 56-57 -1.3448133149017707 2316 58-59 -0.9732663639250418 2316 60-61 -1.010678788631516 2316 62-63 -1.4219854271866197 2316 64-65 -1.6471827139357558 2316 66-67 -1.2329644097192798 2316 68-69 -1.213165793720968 2316 70-71 -1.1493801463997073 2316 72-73 -1.2046329781565284 2316 74-75 -0.9729426600147697 2316 76-77 -0.9790790772843252 2316 78-79 -0.9946373828560624 2316 80-81 -0.9011878730740044 2316 82-83 -0.9688473991015627 2316 84-85 -1.1576957228117486 2316 86-87 -1.299787830569656 2316 88-89 -1.0539435043210368 2316 90-91 -0.940203053895857 2316 92-93 -0.9438566586022077 2316 94-95 -1.2248635374173311 2316 96-97 -1.2138019874326176 2316 98-99 -1.1877011066354903 2316 100-101 -1.4353315347453837 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 40.0 4 85.0 5 181.0 6 463.0 7 1374.0 8 3547.0 9 7015.0 10 12032.0 11 17793.0 12 25224.0 13 35107.0 14 45538.0 15 59731.0 16 77781.0 17 104068.0 18 86503.0 19 49633.0 20 39680.0 21 38861.0 22 40742.0 23 44730.0 24 53356.0 25 66400.0 26 84162.0 27 108150.0 28 137026.0 29 172962.0 30 219048.0 31 277061.0 32 354992.0 33 469055.0 34 656481.0 35 985694.0 36 1570096.0 37 2358056.0 38 2235450.0 39 465769.0 40 137.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.94829437424544 22.20636813681781 17.2132579506286 22.632079538308155 2 14.278022345773362 21.79701089720315 39.27915268492922 24.645814072094268 3 16.445336634628585 26.632595387216966 32.006741010617844 24.915326967536604 4 11.938329081837363 18.972634366913805 38.40072523811205 30.68831131313678 5 12.530405804164566 38.131930122800796 35.412032751646976 13.925631321387666 6 29.26227936334006 38.913207022514904 17.924111709972344 13.900401904172686 7 25.865845172077695 33.81617325267323 22.37637320813923 17.941608367109843 8 25.85399871075154 35.23389111197175 19.83334230555372 19.078767871722988 9 25.111486467277093 16.645017146896546 21.658684590830497 36.58481179499586 10-11 23.65104145506663 27.519742025489123 28.274711085990923 20.554505433453325 12-13 24.67085099194536 25.086724832328017 28.625288746507255 21.617135429219367 14-15 21.810348393195763 26.806724484735977 26.98587936708941 24.39704775497885 16-17 20.480353682143885 29.9781466078407 27.57810691608642 21.963392793928996 18-19 20.676322858086415 27.99429164813757 31.013493827003114 20.3158916667729 20-21 21.643724032110686 26.96597748811064 30.980909111877263 20.40938936790141 22-23 21.216411953454266 27.000962396648287 31.1608242385291 20.62180141136835 24-25 20.817320608841275 27.01377210854205 31.03139561655522 21.13751166606146 26-27 20.78110465149606 27.414035083696664 30.797005928298653 21.007854336508622 28-29 20.67423499418955 27.072571299356436 31.46990358749217 20.783290118961848 30-31 21.019663682079518 27.355758379861754 30.812628755430737 20.81194918262799 32-33 20.5725112566693 27.218600693330487 31.021233544223342 21.18765450577687 34-35 21.1100280405024 27.29878603050474 30.716287035030316 20.874898893962545 36-37 21.357902627576017 27.12022534723507 30.444869291624265 21.077002733564644 38-39 21.02388263109722 27.606802803441756 30.531867964569955 20.83744660089107 40-41 20.82325123608102 27.157785861390177 30.82643269108723 21.192530211441575 42-43 20.829276486222966 27.421143856864667 30.373291991514407 21.37628766539796 44-45 20.897637876875798 27.6491466548263 30.14880721331889 21.304408254979016 46-47 21.44744595200003 27.777037985723542 29.570096749210034 21.205419313066393 48-49 21.260639502310337 27.828909181669587 29.422166885871132 21.488284430148948 50-51 21.252746728687402 28.050281184312375 28.98437528659523 21.71259680040499 52-53 21.39911120958843 27.92218553371446 28.98964288439415 21.68906037230296 54-55 21.419210463591014 28.38467240703661 28.925485945772095 21.27063118360028 56-57 21.632519053201314 27.971555412081294 28.94360169873324 21.452323835984156 58-59 21.28953562097253 27.8963945950405 29.05357174054885 21.76049804343812 60-61 21.589182351728383 27.91013290572234 28.950100556085058 21.55058418646422 62-63 21.553142031423707 27.851681560330388 29.174456805746136 21.420719602499766 64-65 21.50494343245541 28.13712308651808 28.849844388410528 21.508089092615982 66-67 21.412849976243727 28.093110827948603 28.930594496514928 21.56344469929274 68-69 21.555645426933552 28.375886370987185 28.70090454334362 21.36756365873564 70-71 21.684338236899066 27.82320805490507 28.783238745218853 21.70921496297701 72-73 21.162301874422027 28.144072603984903 28.738287126405147 21.95533839518792 74-75 21.370244750105986 28.03532479538431 28.775983504640294 21.818446949869415 76-77 21.439582318684682 28.43990711324857 28.498487524848525 21.62202304321822 78-79 21.415730497831525 28.1939083520723 28.759461539329273 21.630899610766903 80-81 21.380345371790472 28.222479040929322 28.665314768834964 21.731860818445238 82-83 21.324285170646032 28.102144177396614 28.923382696021655 21.650187955935696 84-85 21.02768337910951 28.143523800959862 28.880943866135254 21.947848953795372 86-87 21.32918732973376 28.25112220723011 28.633604475328685 21.786085987707445 88-89 21.221582636823154 28.274389625968645 28.67461835430239 21.829409382905812 90-91 21.17101695749973 28.18994604411061 28.760356264960805 21.87868073342885 92-93 21.337632482115083 28.300065324537677 28.459740394803053 21.902561798544188 94-95 21.093825875426162 28.107570862919257 28.825607175364947 21.972996086289633 96-97 21.23552102306686 28.367610818746407 28.63503564151097 21.761832516675764 98-99 20.959658164744827 28.366146776319734 28.43851952583124 22.235675533104192 100-101 20.903281879254333 28.468679507121543 28.287365759457494 22.34067285416663 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4584.0 1 3017.5 2 1858.0 3 2401.0 4 2074.0 5 1930.5 6 4212.0 7 9851.0 8 16292.5 9 19362.0 10 16308.0 11 12387.0 12 12279.5 13 13331.5 14 14250.5 15 14889.5 16 15672.0 17 16531.0 18 17676.0 19 19270.5 20 21807.5 21 25698.0 22 33212.0 23 46380.5 24 58677.0 25 70504.5 26 90616.5 27 118802.0 28 156704.5 29 187669.5 30 201772.5 31 221488.0 32 248924.0 33 272286.5 34 293460.0 35 314754.0 36 327050.0 37 336823.5 38 350395.0 39 357329.0 40 367018.5 41 369639.5 42 363614.0 43 364441.0 44 362346.5 45 355780.0 46 348574.5 47 341933.0 48 333346.5 49 328485.0 50 337569.0 51 330277.5 52 294764.0 53 272234.5 54 270961.5 55 275286.5 56 278654.5 57 257869.0 58 219705.5 59 190529.0 60 158267.0 61 123027.5 62 94602.0 63 72052.5 64 55549.0 65 45010.0 66 37201.0 67 29578.0 68 22017.5 69 15586.0 70 11426.0 71 7983.0 72 5635.0 73 3947.5 74 2808.0 75 1983.0 76 1270.0 77 868.5 78 610.0 79 426.5 80 274.0 81 175.5 82 120.5 83 85.5 84 60.0 85 41.0 86 36.0 87 34.0 88 24.0 89 17.0 90 16.0 91 10.0 92 4.5 93 3.0 94 2.5 95 2.0 96 3.0 97 1.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04888101901425786 2 0.0020267739591277646 3 2.2927307229952086E-4 4 2.1093122651555922E-4 5 0.0024853201037268063 6 0.0016966207350164544 7 0.0 8 9.170922891980834E-6 9 1.2839292048773168E-4 10-11 4.5854614459904174E-5 12-13 0.0 14-15 5.044007590589459E-4 16-17 0.0 18-19 4.5854614459904174E-5 20-21 5.181571433969172E-4 22-23 0.0 24-25 6.37379140992668E-4 26-27 0.007478887618410371 28-29 0.007579767770222161 30-31 0.0010913398241457193 32-33 0.006548038944874316 34-35 0.007987873838915307 36-37 0.004580875984544427 38-39 0.0026412257928904804 40-41 0.0019442356530999369 42-43 0.0012610018976473647 44-45 8.02455753048323E-4 46-47 5.777681421947926E-4 48-49 0.0018800391928560712 50-51 0.0013572965880131636 52-53 0.0020176030362357837 54-55 7.795284458183711E-4 56-57 1.8800391928560712E-4 58-59 7.290883699124764E-4 60-61 0.006052809108707351 62-63 0.002622883947106519 64-65 0.0010684125169157673 66-67 0.005424600890606664 68-69 0.007258785469002831 70-71 0.0024027817976989786 72-73 4.906443747209747E-4 74-75 0.0032602630880991866 76-77 0.0019488211145459275 78-79 0.002558687486862653 80-81 8.941649819681315E-4 82-83 8.712376747381794E-5 84-85 6.098663723167256E-4 86-87 0.004732196212262111 88-89 0.0024119527205909595 90-91 0.0011096816699296811 92-93 8.253830602782751E-5 94-95 4.860589132749842E-4 96-97 2.659567638674442E-4 98-99 5.685972193028118E-4 100-101 9.170922891980834E-6 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.0904028E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.65696661607765 #Duplication Level Percentage of deduplicated Percentage of total 1 62.01036893750464 22.111016550552616 2 18.202770490162283 12.9811115937568 3 7.669612026926427 8.204253000071498 4 3.8490330002073048 5.489793647702923 5 2.147768196537499 3.8291449441505447 6 1.4079701310742132 3.012236637608862 7 0.9197602673304485 2.2957102802897524 8 0.6423425919914161 1.8323190686978172 9 0.4677143632961522 1.5009547894119823 >10 2.352842297969945 14.948987643859274 >50 0.17966640389732064 4.442837805379128 >100 0.12795607127638467 9.022354045947928 >500 0.013607201860000806 3.3788144847538213 >1k 0.008354509125809373 5.4284385211254715 >5k 1.037825956764829E-4 0.2351251283553572 >10k+ 1.2972824459560363E-4 1.2869018583362288 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 47625 0.4367652027305873 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 40065 0.36743302566721214 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27075 0.2483027373003811 No Hit GATCAGCACCATGAAGCTTCTCACGGGCCTGGTTTTCTGCTCCTTGGTCC 13977 0.12818198926121613 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 9.170922891980834E-6 0.0 0.0 0.0 0.0 7 9.170922891980834E-6 0.0 0.0 0.0 0.0 8 9.170922891980834E-6 0.0 0.0 9.170922891980834E-6 0.0 9 9.170922891980834E-6 0.0 0.0 9.170922891980834E-6 0.0 10-11 9.170922891980834E-6 0.0 0.0 9.170922891980834E-6 0.0 12-13 9.170922891980834E-6 0.0 0.0 9.170922891980834E-6 0.0 14-15 9.170922891980834E-6 0.0 0.0 1.834184578396167E-5 0.0 16-17 9.170922891980834E-6 0.0 0.0 3.209823012193292E-5 0.0 18-19 9.170922891980834E-6 0.0 0.0 3.668369156792334E-5 0.0 20-21 9.170922891980834E-6 0.0 0.0 4.5854614459904174E-5 0.0 22-23 9.170922891980834E-6 0.0 0.0 9.170922891980835E-5 0.0 24-25 9.170922891980834E-6 0.0 0.0 1.7883299639362627E-4 0.0 26-27 9.170922891980834E-6 0.0 0.0 4.2644791447710883E-4 0.0 28-29 9.170922891980834E-6 0.0 0.0 0.0011371944386056236 0.0 30-31 9.170922891980834E-6 0.0 0.0 0.002870498865190001 0.0 32-33 9.170922891980834E-6 0.0 0.0 0.00581436511351585 0.0 34-35 9.170922891980834E-6 0.0 0.0 0.010404412020952257 0.0 36-37 9.170922891980834E-6 0.0 0.0 0.018346431245407663 0.0 38-39 9.170922891980834E-6 0.0 0.0 0.030557515076080143 0.0 40-41 9.170922891980834E-6 0.0 0.0 0.04352978550678703 0.0 42-43 9.170922891980834E-6 0.0 0.0 0.05608936440735479 0.0 44-45 9.170922891980834E-6 9.170922891980834E-6 0.0 0.07222101777434907 0.0 46-47 9.170922891980834E-6 9.170922891980834E-6 0.0 0.09294271804877977 0.0 48-49 9.170922891980834E-6 9.170922891980834E-6 0.0 0.11586543981728586 0.0 50-51 9.170922891980834E-6 9.170922891980834E-6 0.0 0.13979237764246388 0.0 52-53 9.170922891980834E-6 1.375638433797125E-5 0.0 0.16396234492427936 0.0 54-55 9.170922891980834E-6 2.2927307229952087E-5 0.0 0.19156682282914167 0.0 56-57 9.170922891980834E-6 2.7512768675942505E-5 0.0 0.2256780705258644 0.0 58-59 9.170922891980834E-6 2.7512768675942505E-5 0.0 0.26447565982038934 0.0 60-61 9.170922891980834E-6 2.7512768675942505E-5 0.0 0.3069186909644766 0.0 62-63 9.170922891980834E-6 2.7512768675942505E-5 0.0 0.34903615434589863 0.0 64-65 9.170922891980834E-6 2.7512768675942505E-5 0.0 0.39546853694799755 4.585461445990417E-6 66-67 9.170922891980834E-6 2.7512768675942505E-5 0.0 0.4505811980673564 9.170922891980834E-6 68-69 9.170922891980834E-6 2.7512768675942505E-5 0.0 0.5162679332811692 9.170922891980834E-6 70-71 9.170922891980834E-6 2.7512768675942505E-5 0.0 0.5884018272880444 9.170922891980834E-6 72-73 9.170922891980834E-6 2.7512768675942505E-5 0.0 0.6681017326808039 9.170922891980834E-6 74-75 9.170922891980834E-6 2.7512768675942505E-5 0.0 0.7521257282171322 9.170922891980834E-6 76-77 9.170922891980834E-6 2.7512768675942505E-5 0.0 0.8429728903850944 9.170922891980834E-6 78-79 9.170922891980834E-6 2.7512768675942505E-5 0.0 0.9507908453646671 9.170922891980834E-6 80-81 9.170922891980834E-6 2.7512768675942505E-5 0.0 1.0733648152774369 9.170922891980834E-6 82-83 9.170922891980834E-6 2.7512768675942505E-5 0.0 1.203931244490568 9.170922891980834E-6 84-85 9.170922891980834E-6 2.7512768675942505E-5 0.0 1.3368225026568163 9.170922891980834E-6 86-87 1.834184578396167E-5 2.7512768675942505E-5 0.0 1.4817184988886676 9.170922891980834E-6 88-89 1.834184578396167E-5 2.7512768675942505E-5 0.0 1.6495509732733629 9.170922891980834E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 22820 0.0 62.09522 1 GTATCAA 44180 0.0 43.571518 1 GTGGTAT 7795 0.0 35.78394 1 ATCAACG 57285 0.0 33.265568 3 TGGTATC 8300 0.0 33.019966 2 TCAACGC 58225 0.0 32.71205 4 TATCAAC 59680 0.0 32.01048 2 CAACGCA 60640 0.0 31.401459 5 AACGCAG 65140 0.0 29.370188 6 CGCAGAG 71695 0.0 26.088064 8 ACGCAGA 73815 0.0 25.583311 7 GTACATG 42910 0.0 21.84916 1 TACATGG 41520 0.0 21.175222 2 GAGTACT 50410 0.0 21.133701 12-13 AGAGTAC 72820 0.0 21.077274 10-11 AGTACTT 50885 0.0 20.819748 12-13 GCAGAGT 88335 0.0 20.75475 9 GTACTTT 50925 0.0 20.691662 14-15 CAGAGTA 79235 0.0 19.784437 10-11 ACATGGG 43185 0.0 19.654707 3 >>END_MODULE