FastQCFastQC Report
Wed 25 May 2016
SRR2049405_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049405_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6855780
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT348710.508636508172666No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT287850.4198646981087491No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151700.22127314470417664No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73930.10783601574146195No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA155200.061.5229871
GTATCAA314450.044.853021
GTGGTAT55150.038.675321
ATCAACG383550.036.3369943
TCAACGC389200.035.799644
TGGTATC58250.035.144762
TATCAAC399200.034.945612
CAACGCA400900.034.7422375
AACGCAG419850.033.3778046
TATAACG6900.033.042382
ACGCAGA468900.029.5439727
CGCAGAG469100.029.2984478
TACCTGG51000.028.6853242
GTACCTG54550.027.6927071
GCAGAGT507200.026.3679
GTACATG287450.024.4751761
TACATGG275150.023.8398422
GTACCGG6050.023.5558591
CAGAGTA487100.022.71789710-11
GAGTACT336100.022.35264812-13
Produced by FastQC (version 0.11.5)