FastQCFastQC Report
Wed 25 May 2016
SRR2049405_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049405_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6855780
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT328910.4797557681255816No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT276940.4039511186181587No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187060.2728500622832121No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72490.10573559828349217No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA154700.059.281061
GTATCAA300900.043.332211
GTGGTAT59450.036.6068651
TGGTATC59950.035.4162452
TATAACG6550.034.083232
ATCAACG396950.032.6309973
TCAACGC403250.032.097644
TATCAAC412550.031.3743042
CAACGCA414300.031.2644795
AACGCAG439050.029.6636776
CGCAGAG497400.025.877698
ACGCAGA500250.025.7967227
TACCTGG46950.024.3817922
GCAGAGT540600.023.4407659
GTACCTG50900.022.6849291
GTACATG273600.022.039131
AGAGTAC502800.021.51419610-11
TACATGG261400.021.459942
AGTACTT364050.020.98563212-13
GAGTACT364800.020.48027812-13
Produced by FastQC (version 0.11.5)