FastQCFastQC Report
Wed 25 May 2016
SRR2049404_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049404_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6954637
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262530.3774891486068935No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228090.3279682318430135No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113010.16249590021736576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA148200.059.8100851
GTATCAA275400.043.0000841
ATCAACG341400.034.2407653
TCAACGC343200.034.1306274
TATCAAC349800.033.443272
CAACGCA358000.032.798545
AACGCAG376000.031.3926286
GTGGTAT60250.029.8020211
TGGTATC59450.028.6818222
ACGCAGA419900.027.8055427
CGCAGAG417550.027.7456918
GCAGAGT443500.025.3832329
GAGTACT274900.023.31878912-13
GTACATG265950.023.237441
CAGAGTA428700.022.61992810-11
TACATGG264250.022.270012
ATAACGC6850.022.189873
GTACTTT295150.021.5247714-15
TATAACG8000.021.3735492
AGAGTAC413100.021.32966610-11
Produced by FastQC (version 0.11.5)