##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049404_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6954637 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.511732244256603 31.0 31.0 34.0 30.0 34.0 2 31.552011557181203 33.0 31.0 34.0 30.0 34.0 3 31.573107697784945 33.0 31.0 34.0 28.0 34.0 4 35.19326630563177 37.0 35.0 37.0 33.0 37.0 5 35.21603758758365 37.0 35.0 37.0 33.0 37.0 6 35.305379993233295 37.0 35.0 37.0 33.0 37.0 7 35.251442742446514 37.0 35.0 37.0 33.0 37.0 8 35.223547541014725 37.0 35.0 37.0 33.0 37.0 9 36.81054266383709 39.0 37.0 39.0 33.0 39.0 10-11 36.746811228249584 39.0 37.0 39.0 33.0 39.0 12-13 36.70971669693185 39.0 37.0 39.0 33.0 39.0 14-15 37.89143660553383 40.0 38.0 41.0 33.0 41.0 16-17 37.88549014132585 40.0 38.0 41.0 33.0 41.0 18-19 37.92121975597001 40.0 38.0 41.0 33.0 41.0 20-21 37.88808121832958 40.0 38.0 41.0 33.0 41.0 22-23 37.7886152073789 40.0 38.0 41.0 33.0 41.0 24-25 37.68729244962749 40.0 38.0 41.0 32.0 41.0 26-27 37.55281368387739 40.0 37.5 41.0 32.0 41.0 28-29 37.39625044412814 40.0 37.0 41.0 32.0 41.0 30-31 37.20684357788912 40.0 37.0 41.0 31.5 41.0 32-33 36.961095668975965 40.0 36.5 41.0 31.0 41.0 34-35 36.765773612627086 40.0 36.0 41.0 30.5 41.0 36-37 36.58094743118872 39.0 36.0 41.0 30.0 41.0 38-39 36.379004684212845 39.0 35.5 41.0 30.0 41.0 40-41 36.16287737232008 39.0 35.0 41.0 30.0 41.0 42-43 35.93020138937517 39.0 35.0 40.0 29.0 41.0 44-45 35.642963392625674 39.0 35.0 40.0 27.5 41.0 46-47 35.27694277932838 39.0 35.0 40.0 26.5 41.0 48-49 34.97269886264373 38.5 34.5 40.0 25.5 41.0 50-51 33.612926814152914 37.0 32.5 39.0 23.5 40.0 52-53 33.89587961240824 37.5 33.5 39.5 23.5 40.0 54-55 34.94662295098939 39.0 35.0 40.0 26.0 41.0 56-57 35.06950125506191 39.0 35.0 40.5 26.0 41.0 58-59 34.91930973536074 39.0 35.0 41.0 26.0 41.0 60-61 34.67425057555125 38.0 34.0 40.0 25.5 41.0 62-63 34.41458267340194 38.0 34.0 40.0 24.5 41.0 64-65 34.145605946075975 38.0 34.0 40.0 23.5 41.0 66-67 33.83482746834953 37.0 34.0 40.0 23.0 41.0 68-69 33.499063718207005 36.5 33.5 40.0 22.0 41.0 70-71 33.16807843457538 36.0 33.0 39.0 21.5 41.0 72-73 32.91216795355386 36.0 33.0 39.0 21.0 41.0 74-75 32.709318257732214 35.5 33.0 39.0 20.5 40.5 76-77 32.36244062774233 35.0 33.0 38.0 19.5 40.0 78-79 31.984569575665848 35.0 32.5 37.0 18.0 39.0 80-81 31.63567091999194 35.0 32.0 37.0 17.5 39.0 82-83 31.283309179185053 35.0 32.0 36.0 14.0 39.0 84-85 30.951512206891604 35.0 32.0 36.0 9.5 37.0 86-87 30.64161335810913 35.0 32.0 35.5 7.0 37.0 88-89 30.344795997260533 35.0 31.0 35.0 2.0 37.0 90-91 30.072996477026766 35.0 31.0 35.0 2.0 36.0 92-93 29.813292052482396 34.0 31.0 35.0 2.0 36.0 94-95 29.551565811414743 34.0 31.0 35.0 2.0 36.0 96-97 29.289348674848164 34.0 31.0 35.0 2.0 35.5 98-99 28.975942885300846 34.0 30.0 35.0 2.0 35.0 100-101 28.443046775841786 34.0 29.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.33978063870000597 1101 2 0.3079638295421887 1101 3 0.31033575169011485 1101 4 0.23381955614089378 1101 5 0.2919435948221505 1101 6 0.2793584107444218 1101 7 0.2832319425474097 1101 8 0.28702003960081157 1101 9 0.36549459803506323 1101 10-11 0.3565659596818236 1101 12-13 0.2764201847970753 1101 14-15 0.4008829812359309 1101 16-17 0.38174707491938165 1101 18-19 0.37351539368156494 1101 20-21 0.356358655330304 1101 22-23 0.40608314840546456 1101 24-25 0.32415065705686885 1101 26-27 0.3041421018822277 1101 28-29 0.2758439784706468 1101 30-31 0.2015455154920005 1101 32-33 0.18396217950292737 1101 34-35 0.18537081508348052 1101 36-37 0.1443223646519698 1101 38-39 0.14714227105129396 1101 40-41 0.2330398857362752 1101 42-43 0.13822911993719345 1101 44-45 0.1665914761854097 1101 46-47 0.1095442943765903 1101 48-49 0.028969738477691465 1101 50-51 0.054162579822509826 1101 52-53 0.12176325176557157 1101 54-55 0.4200644830312825 1101 56-57 0.38288068073472914 1101 58-59 0.27496705105164665 1101 60-61 0.33756650991310977 1101 62-63 0.28388392021275166 1101 64-65 0.1990758533724204 1101 66-67 0.18844961926037485 1101 68-69 0.18621405696585214 1101 70-71 0.0849049713184229 1101 72-73 0.11559115027782951 1101 74-75 0.07578812451981065 1101 76-77 0.002336559720433229 1101 78-79 0.025696253511977574 1101 80-81 -0.1306056812764922 1101 82-83 -0.05967590529123967 1101 84-85 -0.1371999215594144 1101 86-87 -0.18289764549408716 1101 88-89 -0.12462333775260248 1101 90-91 -0.205495961721617 1101 92-93 -0.32629029843275603 1101 94-95 -0.40475057772307466 1101 96-97 -0.4717191865962391 1101 98-99 -0.5163309369319471 1101 100-101 -0.6690245831380288 1102 1 0.2122027229893959 1102 2 0.2343543814426674 1102 3 0.2523822274557759 1102 4 0.2285668832220935 1102 5 0.2710345701089949 1102 6 0.2429018408593393 1102 7 0.2513055209593773 1102 8 0.254680914111006 1102 9 0.3612416802732099 1102 10-11 0.2909368275611399 1102 12-13 0.2747875336571681 1102 14-15 0.3454307030074588 1102 16-17 0.3002379754897859 1102 18-19 0.2331365736620299 1102 20-21 0.3052809280999682 1102 22-23 0.24009915264115733 1102 24-25 0.20275762418071253 1102 26-27 0.2008486148358415 1102 28-29 0.17726378214530314 1102 30-31 0.15018776492556896 1102 32-33 0.22349928761749993 1102 34-35 0.19873553859810045 1102 36-37 0.09413595757885673 1102 38-39 0.07259740356732891 1102 40-41 0.1417784719362274 1102 42-43 -0.037863475016521875 1102 44-45 0.04283847023635445 1102 46-47 0.13885274664664848 1102 48-49 0.036689490636433675 1102 50-51 -0.12885295191181 1102 52-53 0.07351754852918191 1102 54-55 0.2281679908094958 1102 56-57 0.2098774190811028 1102 58-59 0.24104082635608393 1102 60-61 0.24290611101240245 1102 62-63 0.2546912115222213 1102 64-65 0.1343730640332339 1102 66-67 0.07038151721567232 1102 68-69 0.027858371653159963 1102 70-71 0.05296976592109104 1102 72-73 0.030701646882647537 1102 74-75 0.0017465805702840953 1102 76-77 -0.03611533127717337 1102 78-79 -0.09101678828942283 1102 80-81 -0.0991697678703467 1102 82-83 -0.20531448158349974 1102 84-85 -0.18999823937434712 1102 86-87 -0.158757586618691 1102 88-89 -0.19588636011588534 1102 90-91 -0.28240070092682856 1102 92-93 -0.36358128515656674 1102 94-95 -0.4556811874088531 1102 96-97 -0.5712874150715024 1102 98-99 -0.6510060804700863 1102 100-101 -0.5876229952820644 1103 1 0.1787360919754093 1103 2 0.20828677782255411 1103 3 0.1808357873255133 1103 4 0.21470303440699468 1103 5 0.19943801758378754 1103 6 0.2536641387441847 1103 7 0.22676160502334142 1103 8 0.19978668738137628 1103 9 0.26811292160173394 1103 10-11 0.2757236202814397 1103 12-13 0.28246762902679023 1103 14-15 0.2869817209727259 1103 16-17 0.339295782491412 1103 18-19 0.3040050775767966 1103 20-21 0.32105920584078973 1103 22-23 0.239822373564067 1103 24-25 0.3175314870001884 1103 26-27 0.30146785440958723 1103 28-29 0.27823999997647064 1103 30-31 0.32197924628278685 1103 32-33 0.19262773052283677 1103 34-35 0.22258075101984076 1103 36-37 0.23865104308659824 1103 38-39 0.2288832263515843 1103 40-41 0.2196604860752771 1103 42-43 0.29187847001256984 1103 44-45 0.10344025367006537 1103 46-47 0.17206704208120271 1103 48-49 0.18074221351952957 1103 50-51 0.16766644235897132 1103 52-53 0.28410818082635814 1103 54-55 0.38594160996793647 1103 56-57 0.4433951492592101 1103 58-59 0.4506785169653824 1103 60-61 0.46077203758929386 1103 62-63 0.3773033430042503 1103 64-65 0.36316555653862537 1103 66-67 0.32892555156400505 1103 68-69 0.3367531734176836 1103 70-71 0.294928016427086 1103 72-73 0.40749035948167744 1103 74-75 0.3008911032984187 1103 76-77 0.24478095954136592 1103 78-79 0.28104104334918745 1103 80-81 0.22629124358997643 1103 82-83 0.24242403052080164 1103 84-85 0.27831279749044313 1103 86-87 0.21579032154031452 1103 88-89 0.1366389189108297 1103 90-91 0.12953621343696042 1103 92-93 0.12624676934869328 1103 94-95 0.11398661836350144 1103 96-97 0.13537348963716767 1103 98-99 0.13517697127286254 1103 100-101 0.11228173312229828 1104 1 0.01899836726467896 1104 2 -0.0019364455392576474 1104 3 0.0583682692744425 1104 4 -0.04343719082446995 1104 5 -0.08031410058727317 1104 6 -0.10866627972634291 1104 7 -0.08718209085306938 1104 8 -0.12558085100014438 1104 9 -0.10441041164429521 1104 10-11 -0.012785184799419369 1104 12-13 -0.09778377009487826 1104 14-15 -0.07870110154244259 1104 16-17 -0.120553822062611 1104 18-19 -0.14653573736247694 1104 20-21 -0.14424232717498597 1104 22-23 -0.11786228742254679 1104 24-25 -0.154200829561141 1104 26-27 -0.19458024269761864 1104 28-29 -0.1933991457454738 1104 30-31 -0.1501621738303882 1104 32-33 -0.1427286697650274 1104 34-35 -0.22253382915634745 1104 36-37 -0.22713112423924997 1104 38-39 -0.22246681536949353 1104 40-41 -0.20691035307344663 1104 42-43 -0.27412826772336985 1104 44-45 -0.27834603213117504 1104 46-47 -0.28345710313558925 1104 48-49 -0.37969086250263473 1104 50-51 -0.436198713119083 1104 52-53 -0.381036258920048 1104 54-55 -0.2255709755789823 1104 56-57 -0.17690399524041567 1104 58-59 -0.12161332353910836 1104 60-61 -0.20857787441602227 1104 62-63 -0.19125461686618905 1104 64-65 -0.2564732848501805 1104 66-67 -0.22248772659023786 1104 68-69 -0.2910687368215008 1104 70-71 -0.3054322566245844 1104 72-73 -0.2783998086873183 1104 74-75 -0.3141057847706037 1104 76-77 -0.3787306456537465 1104 78-79 -0.3079725306567731 1104 80-81 -0.3332503483965823 1104 82-83 -0.3588320296373766 1104 84-85 -0.28687853949239184 1104 86-87 -0.3245007032516085 1104 88-89 -0.34023263629382683 1104 90-91 -0.4154770886648116 1104 92-93 -0.46555538168784594 1104 94-95 -0.43661755773562305 1104 96-97 -0.4817047127518457 1104 98-99 -0.4753041868893142 1104 100-101 -0.5079889736913934 1105 1 0.2683463566843045 1105 2 0.30309813321026624 1105 3 0.30932002491108435 1105 4 0.269151193403772 1105 5 0.26502380732421926 1105 6 0.3065293597731653 1105 7 0.2846714185131347 1105 8 0.33610893375372797 1105 9 0.35656479203522906 1105 10-11 0.3553591989614304 1105 12-13 0.3099983404953619 1105 14-15 0.4242386521834831 1105 16-17 0.4037616107211832 1105 18-19 0.38767993983068294 1105 20-21 0.4062084104424386 1105 22-23 0.35224463637948844 1105 24-25 0.3861424272648506 1105 26-27 0.4106610578241927 1105 28-29 0.4374488040189277 1105 30-31 0.44686550067226705 1105 32-33 0.3904312703465038 1105 34-35 0.45304848207116777 1105 36-37 0.43498457577836547 1105 38-39 0.3660905639227323 1105 40-41 0.46561320848629606 1105 42-43 0.5120446520574404 1105 44-45 0.4632726093353554 1105 46-47 0.4682875148029808 1105 48-49 0.4538551862932749 1105 50-51 0.4251851209900934 1105 52-53 0.47213691574395966 1105 54-55 0.618362986062607 1105 56-57 0.6034142697133902 1105 58-59 0.7016734951251209 1105 60-61 0.6257884320843488 1105 62-63 0.6392988852954744 1105 64-65 0.6500379817739841 1105 66-67 0.5985426493110069 1105 68-69 0.596969370940208 1105 70-71 0.5923909743135098 1105 72-73 0.6043732962319268 1105 74-75 0.5349150641902227 1105 76-77 0.6160256018602297 1105 78-79 0.6258744437707513 1105 80-81 0.5778100080645281 1105 82-83 0.5688753777767594 1105 84-85 0.5945385081368642 1105 86-87 0.5809912425000441 1105 88-89 0.5689443694885732 1105 90-91 0.5471634225061557 1105 92-93 0.5302584797198655 1105 94-95 0.5442996221786096 1105 96-97 0.4778093927019853 1105 98-99 0.5187753185041011 1105 100-101 0.4927312378192745 1106 1 0.2150480635621399 1106 2 0.2635454337089982 1106 3 0.17907658174166485 1106 4 0.2220273025463797 1106 5 0.22836740379456444 1106 6 0.2591842367850461 1106 7 0.29787954748358203 1106 8 0.2956274532123544 1106 9 0.30887724962092733 1106 10-11 0.32673768394780467 1106 12-13 0.3355073316581567 1106 14-15 0.40291065285972394 1106 16-17 0.37086383216469443 1106 18-19 0.36178092856024335 1106 20-21 0.37490946009524606 1106 22-23 0.41499233301195915 1106 24-25 0.39766316885231845 1106 26-27 0.37803379021243444 1106 28-29 0.42540299787410873 1106 30-31 0.4130300077833411 1106 32-33 0.45234955751138983 1106 34-35 0.4604620897484182 1106 36-37 0.41833465727631847 1106 38-39 0.45750891370105506 1106 40-41 0.4416907258292895 1106 42-43 0.49818485498607856 1106 44-45 0.440475178883851 1106 46-47 0.469910610617454 1106 48-49 0.4948743224103396 1106 50-51 0.41608556289364174 1106 52-53 0.5399576874111958 1106 54-55 0.5495830259957302 1106 56-57 0.6392351843133071 1106 58-59 0.6326253554114061 1106 60-61 0.5949810941795377 1106 62-63 0.6118800486778326 1106 64-65 0.6462704838006559 1106 66-67 0.5540362332074196 1106 68-69 0.6544568455901043 1106 70-71 0.6690243735602692 1106 72-73 0.5886599867055651 1106 74-75 0.5680489369014694 1106 76-77 0.5826648797961838 1106 78-79 0.6641373627635154 1106 80-81 0.5841264386239793 1106 82-83 0.6460199040370327 1106 84-85 0.5979522417041991 1106 86-87 0.604396388048297 1106 88-89 0.6620533925740695 1106 90-91 0.6164162843401186 1106 92-93 0.5729100669890634 1106 94-95 0.6098054049931712 1106 96-97 0.5416904719104281 1106 98-99 0.6821894829595969 1106 100-101 0.7439312281886039 1107 1 0.313633141291227 1107 2 0.2955749195568913 1107 3 0.27098214684583155 1107 4 0.3129303137238111 1107 5 0.30263660800486036 1107 6 0.2619706510417501 1107 7 0.3317837843260989 1107 8 0.39184809241677954 1107 9 0.36754276694712473 1107 10-11 0.40076944066458964 1107 12-13 0.3440494914055492 1107 14-15 0.4476464866750902 1107 16-17 0.44625937593246334 1107 18-19 0.3799935871294693 1107 20-21 0.47925618480200427 1107 22-23 0.5248810004893869 1107 24-25 0.47910756206210436 1107 26-27 0.48736029252282265 1107 28-29 0.45781432351872553 1107 30-31 0.4903860876476145 1107 32-33 0.530648671700142 1107 34-35 0.5558183649441517 1107 36-37 0.5189419940554103 1107 38-39 0.5940384173213644 1107 40-41 0.5121122964164826 1107 42-43 0.5322118194624821 1107 44-45 0.5657754631394525 1107 46-47 0.5896389496414187 1107 48-49 0.4955340271212876 1107 50-51 0.5039861440367446 1107 52-53 0.6018577825746121 1107 54-55 0.655194852855189 1107 56-57 0.6193044262271243 1107 58-59 0.6469798295082541 1107 60-61 0.665136292221888 1107 62-63 0.7161158972566284 1107 64-65 0.7224612723521062 1107 66-67 0.7249760552981641 1107 68-69 0.7201738827617632 1107 70-71 0.700993825367064 1107 72-73 0.6929955878858678 1107 74-75 0.7123591240269533 1107 76-77 0.6590729750755315 1107 78-79 0.6623491345566599 1107 80-81 0.6904591495898522 1107 82-83 0.7104879807876543 1107 84-85 0.7009071278840295 1107 86-87 0.6840605996510831 1107 88-89 0.7676225501296194 1107 90-91 0.6936188381853157 1107 92-93 0.7029311428685752 1107 94-95 0.7378618583846936 1107 96-97 0.718625923290027 1107 98-99 0.7458284299083573 1107 100-101 0.8370596143972868 1108 1 0.24435399822450066 1108 2 0.24213114973609962 1108 3 0.2464768330527356 1108 4 0.2697956838282778 1108 5 0.269252037962616 1108 6 0.24457113984100687 1108 7 0.26559693505466697 1108 8 0.2939039209536034 1108 9 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-0.41545483208275513 2316 90-91 -0.4555019782269589 2316 92-93 -0.5526598972195522 2316 94-95 -0.8597485174098374 2316 96-97 -0.654141561946382 2316 98-99 -0.4579352999586348 2316 100-101 -0.4448572266962252 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 59771.0 3 12920.0 4 10527.0 5 9206.0 6 9510.0 7 10372.0 8 11756.0 9 14074.0 10 16940.0 11 20178.0 12 24497.0 13 29147.0 14 33031.0 15 40130.0 16 49644.0 17 63201.0 18 51571.0 19 31317.0 20 28471.0 21 28858.0 22 30549.0 23 33116.0 24 38233.0 25 45089.0 26 53863.0 27 65945.0 28 80324.0 29 98777.0 30 123767.0 31 156539.0 32 202205.0 33 271555.0 34 381914.0 35 574015.0 36 903887.0 37 1391226.0 38 1540537.0 39 407809.0 40 166.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.38632805978001 20.612560721890684 15.461408711911433 21.53970250641787 2 15.296850107580905 20.715237951305244 40.89973959999718 23.08817234111667 3 17.910912412049615 26.52339608593014 31.341130950181235 24.224560551839012 4 12.438963258674992 17.840480295901205 39.42551083781874 30.295045607605058 5 12.773133023919536 37.80707895861076 35.3744894808943 14.045298536575407 6 28.993163804111493 38.76327263105824 18.2152840889482 14.02827947588206 7 25.77086423644323 33.93681990391302 22.751753820386476 17.540562039257278 8 25.107008824570237 35.56574009326272 20.641749539459788 18.685501542707257 9 25.173572763082813 17.142661567912825 22.087256336628197 35.596509332376165 10-11 23.492633281536655 27.494077606435557 28.781811744817524 20.23147736721026 12-13 24.695694989424435 25.107695188158935 29.09476651680331 21.101843305613322 14-15 21.959035947078032 26.761707353197046 27.26275057913059 24.01650612059433 16-17 20.470212414876052 30.29556609515332 27.815466845743163 21.418754644227462 18-19 20.49100474351814 28.364744449934225 30.983658818704658 20.160591987842974 20-21 21.716765110271314 27.346808014408936 30.854952438495875 20.08147443682388 22-23 21.327864592956818 27.286202413755472 30.905771568196222 20.480161425091488 24-25 21.032928357141188 27.509009956338787 30.787314700474 20.670746986046026 26-27 20.87700407423829 27.582224389308212 30.746641246383753 20.79413029006975 28-29 20.770355264929734 27.551780516136837 30.915230494823515 20.76263372410992 30-31 21.032983451872493 27.84243967674027 30.6163616895797 20.508215181807536 32-33 20.782713781921707 27.67239849051004 30.85136755019333 20.693520177374918 34-35 21.0497023947324 27.56325603773319 30.650732747104975 20.736308820429436 36-37 21.309715385262997 27.64326735270758 30.35277020059482 20.694247061434602 38-39 21.105106777059245 27.835074301027667 30.415010338250113 20.644808583662968 40-41 21.072573780203726 27.647409424770363 30.433507227037694 20.84650956798822 42-43 21.032412730421537 27.76554736061686 30.220630654311183 20.98140925465042 44-45 21.18258754315467 27.940003646792604 29.930381636270976 20.947027173781745 46-47 21.46980915046102 28.077412054912287 29.500581095900202 20.952197698726494 48-49 21.461520868582962 28.0307370889207 29.321562818352092 21.186179224144247 50-51 21.40932772724374 28.235050253993133 29.072972705004375 21.282649313758753 52-53 21.409894373795204 28.27627717799086 29.003693634751876 21.31013481346206 54-55 21.437677081321624 28.579442367565854 28.875714526682827 21.10716602442969 56-57 21.336197862761566 28.382790707472672 28.895541570990822 21.38546985877494 58-59 21.249178101348505 28.102761327821536 29.20192877010169 21.446131800728267 60-61 21.457439580704648 28.24875171201484 29.044449477138983 21.249359230141526 62-63 21.258597355597786 28.304352427448038 29.130160138869076 21.306890078085097 64-65 21.391730211633302 28.17197054235064 29.128903422171547 21.307395823844516 66-67 21.27544142567606 28.25743354439708 28.946452310065997 21.52067271986087 68-69 21.522723839694102 28.407058948882174 28.74804002564386 21.322177185779868 70-71 21.53972868224021 28.19247575532584 28.761347654970752 21.506447907463198 72-73 21.40537434655082 28.38851923819244 28.841331455710538 21.3647749595462 74-75 21.492079907087856 28.47624288467066 28.656966893650708 21.374710314590768 76-77 21.530146645872115 28.540046106842105 28.534344406160685 21.39546284112509 78-79 21.584702969930415 28.458174079743188 28.677926598603587 21.279196351722806 80-81 21.52068299194234 28.466352793173535 28.606579148509216 21.40638506637491 82-83 21.35963651102877 28.485131137308063 28.796041652330583 21.359190699332583 84-85 21.3915221623739 28.4527414988163 28.66443794496482 21.491298393844975 86-87 21.474916327843864 28.54021116659297 28.53484752075177 21.450024984811392 88-89 21.37292333389317 28.48029223445937 28.630606797177506 21.516177634469955 90-91 21.286413592001548 28.43478622227546 28.636355204866877 21.642444980856123 92-93 21.37031778564919 28.55475298084912 28.51226288585823 21.56266634764346 94-95 21.35207877785787 28.358268832330175 28.688283327890847 21.60136906192111 96-97 21.36179821714267 28.46925028008801 28.565626581482622 21.603324921286696 98-99 21.358234307622897 28.391561229973917 28.52959986483013 21.720604597573054 100-101 21.394529751846132 28.43643490079123 28.364252083479833 21.804783263882808 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1783.0 1 1165.0 2 871.0 3 1229.5 4 1029.5 5 976.0 6 1615.0 7 3365.0 8 6318.5 9 8291.0 10 7621.5 11 6636.5 12 6964.5 13 7585.5 14 8174.5 15 8838.0 16 9383.5 17 10014.5 18 10904.0 19 12072.0 20 13856.5 21 16386.0 22 20751.5 23 28409.5 24 37365.5 25 47524.5 26 61852.5 27 79386.5 28 101400.0 29 124116.5 30 140594.0 31 156481.5 32 174190.5 33 190738.5 34 204737.0 35 217694.0 36 227742.5 37 235092.5 38 242573.0 39 246146.5 40 246879.0 41 244282.0 42 237336.0 43 230086.5 44 224734.0 45 218742.5 46 212513.0 47 206654.5 48 200998.0 49 197190.5 50 193804.0 51 185591.0 52 174891.5 53 165866.0 54 159212.0 55 153763.5 56 142018.5 57 126824.5 58 114300.0 59 103372.5 60 91736.0 61 79479.0 62 68147.0 63 58162.0 64 48731.5 65 40899.0 66 34095.0 67 27293.0 68 20929.5 69 15826.5 70 12084.5 71 8836.5 72 6684.0 73 5079.0 74 3926.0 75 3015.0 76 2164.0 77 1622.0 78 1256.0 79 927.0 80 688.0 81 528.5 82 402.5 83 328.5 84 291.0 85 244.0 86 192.5 87 165.0 88 149.0 89 127.0 90 108.0 91 97.5 92 87.5 93 74.0 94 58.5 95 51.0 96 40.5 97 30.0 98 19.5 99 9.5 100 5.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01449392685772097 2 0.004831308952573657 3 0.004169879750733216 4 0.006096651773485805 5 0.0028182635556679666 6 0.006858733245171531 7 0.0015097840476792678 8 0.004371184290423785 9 0.011229917535595317 10-11 0.007520162447011973 12-13 0.009137788212382616 14-15 0.0035515872359693254 16-17 0.00631952465671465 18-19 0.004385563186115968 20-21 9.058704286075606E-4 22-23 0.0011503116553746803 24-25 0.007541730790550248 26-27 0.0012293955816816895 28-29 9.56196563530203E-4 30-31 6.254819626099824E-4 32-33 0.001473836808448809 34-35 0.0017686041701385708 36-37 0.0013731845386035246 38-39 0.0017757936179846627 40-41 0.00283264245136015 42-43 0.003005189199666352 44-45 0.008354138397158616 46-47 0.013882823790803172 48-49 0.005284244166877438 50-51 0.0059456733687178786 52-53 0.013192636797578365 54-55 0.008023423796238395 56-57 0.00549992760226019 58-59 0.006678997049019237 60-61 0.0016104363175245523 62-63 0.002487548954747746 64-65 0.0025666328810547553 66-67 0.0026169590159773977 68-69 0.0020921293232126995 70-71 0.0024659806112094706 72-73 0.001416321225680075 74-75 0.0015816785261401852 76-77 0.008217538888082873 78-79 0.006477692509328667 80-81 0.012653428209121482 82-83 0.014788694219410734 84-85 0.008282243918697698 86-87 0.005924105025179604 88-89 0.003177735947972554 90-91 0.0027895057642836 92-93 0.0010784171769137626 94-95 0.0013516161950652493 96-97 0.001818930305061213 98-99 0.006103841221331898 100-101 0.011014234100212563 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6954637.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.36592692041724 #Duplication Level Percentage of deduplicated Percentage of total 1 71.82101168003477 38.32794860667168 2 15.85708055544214 16.924556041857887 3 5.393181602503287 8.634364058031876 4 2.3843128080895193 5.0896425228768045 5 1.2499065619808354 3.335121112200961 6 0.7594359235679318 2.4316801198719493 7 0.5159627558748244 1.9274381506571077 8 0.36552848864867377 1.560541329004455 9 0.2519832990119556 1.21025900882139 >10 1.2889070025990592 12.011932134815249 >50 0.07328606966053128 2.689332248114757 >100 0.03640777610151073 3.5232343360180156 >500 0.0020240258716072235 0.7200976026905873 >1k 8.635116578105394E-4 0.6616957516105721 >5k 2.6984738899414993E-5 0.08289017617042581 >10k+ 8.095421669824497E-5 0.8692668005864164 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26253 0.3774891486068935 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22809 0.3279682318430135 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11301 0.16249590021736576 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 1.4378895692183503E-5 0.0 0.0 0.0 4 0.0 1.4378895692183503E-5 0.0 0.0 0.0 5 0.0 1.4378895692183503E-5 0.0 0.0 0.0 6 0.0 1.4378895692183503E-5 0.0 0.0 0.0 7 0.0 1.4378895692183503E-5 0.0 0.0 0.0 8 0.0 1.4378895692183503E-5 0.0 0.0 0.0 9 0.0 1.4378895692183503E-5 0.0 0.0 0.0 10-11 0.0 1.4378895692183503E-5 0.0 0.0 0.0 12-13 0.0 1.4378895692183503E-5 0.0 0.0 0.0 14-15 0.0 2.1568343538275254E-5 0.0 2.8757791384367007E-5 0.0 16-17 0.0 2.8757791384367007E-5 0.0 4.313668707655051E-5 0.0 18-19 0.0 2.8757791384367007E-5 0.0 4.313668707655051E-5 0.0 20-21 0.0 2.8757791384367007E-5 0.0 6.470503061482576E-5 0.0 22-23 7.189447846091752E-6 2.8757791384367007E-5 0.0 1.5816785261401852E-4 0.0 24-25 1.4378895692183503E-5 2.8757791384367007E-5 0.0 3.810407358428628E-4 0.0 26-27 1.4378895692183503E-5 2.8757791384367007E-5 0.0 6.326714104560741E-4 0.0 28-29 1.4378895692183503E-5 5.0326134922642265E-5 0.0 0.001761414722292479 0.0 30-31 1.4378895692183503E-5 8.627337415310102E-5 0.0 0.0045005943516534365 0.0 32-33 1.4378895692183503E-5 8.627337415310102E-5 0.0 0.009950195818990985 0.0 34-35 1.4378895692183503E-5 8.627337415310102E-5 0.0 0.019188636301218885 0.0 36-37 1.4378895692183503E-5 9.346282199919277E-5 0.0 0.03529299947646441 0.0 38-39 2.1568343538275254E-5 1.1503116553746803E-4 0.0 0.05614239823013049 0.0 40-41 2.8757791384367007E-5 1.2941006122965153E-4 0.0 0.07961594544762005 0.0 42-43 2.8757791384367007E-5 1.4378895692183504E-4 0.0 0.10364308014925869 0.0 44-45 2.8757791384367007E-5 1.5097840476792677E-4 0.0 0.1329904062570052 0.0 46-47 2.8757791384367007E-5 1.8692564399838555E-4 0.0 0.17002325211222383 0.0 48-49 2.8757791384367007E-5 2.1568343538275254E-4 0.0 0.20868091318065918 0.0 50-51 2.8757791384367007E-5 2.228728832288443E-4 0.0 0.24973266038184308 0.0 52-53 2.8757791384367007E-5 2.3006233107493606E-4 0.0 0.29036741960795365 0.0 54-55 2.8757791384367007E-5 2.3006233107493606E-4 0.0 0.3353014686460271 0.0 56-57 2.8757791384367007E-5 2.5882012245930305E-4 0.0 0.38992689338063224 0.0 58-59 2.8757791384367007E-5 2.7319901815148656E-4 0.0 0.4512025573728722 0.0 60-61 3.594723923045876E-5 2.875779138436701E-4 0.0 0.5161448397666191 0.0 62-63 4.313668707655051E-5 2.9476736168976183E-4 0.0 0.5819858031411274 0.0 64-65 4.313668707655051E-5 3.0195680953585353E-4 0.0 0.6536789770623542 0.0 66-67 4.313668707655051E-5 3.3071460092022056E-4 0.0 0.7374863706042458 0.0 68-69 4.313668707655051E-5 3.379040487663123E-4 0.0 0.8304818784934427 0.0 70-71 4.313668707655051E-5 3.594723923045876E-4 0.0 0.9333269299317851 0.0 72-73 4.313668707655051E-5 3.6666184015067934E-4 0.0 1.0407013335131654 0.0 74-75 4.313668707655051E-5 3.954196315350463E-4 0.0 1.1508796217545214 0.0 76-77 4.313668707655051E-5 4.313668707655051E-4 0.0 1.2767309063003576 0.0 78-79 4.313668707655051E-5 4.313668707655051E-4 0.0 1.4223100357358693 0.0 80-81 4.313668707655051E-5 4.313668707655051E-4 0.0 1.5802046893317365 0.0 82-83 4.313668707655051E-5 4.313668707655051E-4 0.0 1.7505514665970345 0.0 84-85 4.313668707655051E-5 4.313668707655051E-4 0.0 1.9184825893860455 0.0 86-87 4.313668707655051E-5 4.313668707655051E-4 0.0 2.1002749676223216 0.0 88-89 4.313668707655051E-5 4.313668707655051E-4 0.0 2.308287262153294 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 14820 0.0 59.810085 1 GTATCAA 27540 0.0 43.000084 1 ATCAACG 34140 0.0 34.240765 3 TCAACGC 34320 0.0 34.130627 4 TATCAAC 34980 0.0 33.44327 2 CAACGCA 35800 0.0 32.79854 5 AACGCAG 37600 0.0 31.392628 6 GTGGTAT 6025 0.0 29.802021 1 TGGTATC 5945 0.0 28.681822 2 ACGCAGA 41990 0.0 27.805542 7 CGCAGAG 41755 0.0 27.745691 8 GCAGAGT 44350 0.0 25.383232 9 GAGTACT 27490 0.0 23.318789 12-13 GTACATG 26595 0.0 23.23744 1 CAGAGTA 42870 0.0 22.619928 10-11 TACATGG 26425 0.0 22.27001 2 ATAACGC 685 0.0 22.18987 3 GTACTTT 29515 0.0 21.52477 14-15 TATAACG 800 0.0 21.373549 2 AGAGTAC 41310 0.0 21.329666 10-11 >>END_MODULE