FastQCFastQC Report
Wed 25 May 2016
SRR2049404_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049404_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6954637
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229150.329492394786385No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201070.2891164556827337No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120370.17307876744681283No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA130050.057.37411
GTATCAA246500.043.3224221
ATCAACG316700.033.2654463
TCAACGC320350.032.9160774
TATCAAC328600.032.1332662
CAACGCA333550.031.7271465
AACGCAG351700.030.1425366
TGGTATC57200.029.396132
GTGGTAT60700.029.1261
CGCAGAG399250.026.2189488
ACGCAGA401350.026.1764327
GCAGAGT434750.023.7395179
GTACATG252200.022.4624821
TACATGG249000.022.0516662
GAGTACT276100.021.27903412-13
AGAGTAC405800.021.27798310-11
ACATGGG258700.020.637153
AGTACTT285650.020.60087612-13
CAGAGTA421500.020.50232310-11
GTACTTT293050.019.82135614-15
Produced by FastQC (version 0.11.5)