##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049404_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6954637 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.09646743604303 33.0 31.0 34.0 30.0 34.0 2 32.28757762626576 34.0 31.0 34.0 30.0 34.0 3 32.355337597059346 34.0 31.0 34.0 30.0 34.0 4 35.98028610263915 37.0 35.0 37.0 35.0 37.0 5 35.868921699292144 37.0 35.0 37.0 35.0 37.0 6 35.89206582600932 37.0 35.0 37.0 35.0 37.0 7 35.821274208847996 37.0 35.0 37.0 35.0 37.0 8 35.812965076394356 37.0 35.0 37.0 35.0 37.0 9 37.429417092509645 39.0 37.0 39.0 34.0 39.0 10-11 37.426661161466804 39.0 37.0 39.0 34.5 39.0 12-13 37.40745929658155 39.0 37.0 39.0 34.0 39.0 14-15 38.645027770680194 40.0 38.0 41.0 34.0 41.0 16-17 38.633037137668005 40.0 38.0 41.0 34.0 41.0 18-19 38.67263942028894 40.0 38.0 41.0 34.0 41.0 20-21 38.667941763171825 40.0 38.0 41.0 34.0 41.0 22-23 38.58807706282873 40.0 38.0 41.0 34.0 41.0 24-25 38.49046060923094 40.0 38.0 41.0 34.0 41.0 26-27 38.31956786529621 40.0 38.0 41.0 34.0 41.0 28-29 38.136808218746715 40.0 38.0 41.0 33.5 41.0 30-31 37.96206164318856 40.0 37.5 41.0 33.0 41.0 32-33 37.7232702440113 40.0 37.0 41.0 33.0 41.0 34-35 37.54463230791197 40.0 37.0 41.0 33.0 41.0 36-37 37.35366734453574 40.0 36.5 41.0 32.0 41.0 38-39 37.50831840684136 40.0 37.0 41.0 33.0 41.0 40-41 37.41916120136824 40.0 37.0 41.0 32.5 41.0 42-43 37.23575055031628 40.0 37.0 41.0 32.0 41.0 44-45 37.011486221351305 40.0 36.0 41.0 31.0 41.0 46-47 36.6902798089965 40.0 36.0 41.0 30.5 41.0 48-49 36.425077901262135 39.5 36.0 41.0 30.0 41.0 50-51 36.19781629149013 39.0 35.5 41.0 30.0 41.0 52-53 36.0215305270426 39.0 35.0 41.0 29.0 41.0 54-55 35.92046321037317 39.0 35.0 40.5 29.0 41.0 56-57 35.8339834271724 39.0 35.0 41.0 29.0 41.0 58-59 35.60050510184787 39.0 35.0 40.0 28.0 41.0 60-61 35.3660145741611 38.0 35.0 40.0 28.0 41.0 62-63 35.09716474058962 38.0 34.0 40.0 27.5 41.0 64-65 34.84789961000121 38.0 34.0 40.0 27.0 41.0 66-67 34.51991872472999 37.0 34.0 40.0 26.0 41.0 68-69 34.21161377653499 37.0 34.0 39.5 26.0 41.0 70-71 33.88712674723354 36.0 34.0 39.0 26.0 41.0 72-73 33.525902214594375 36.0 33.0 39.0 26.0 40.5 74-75 33.15274528347058 35.0 33.0 38.5 25.0 40.0 76-77 31.114025577467235 33.5 30.0 36.0 22.0 38.5 78-79 32.43222565031073 35.0 32.5 37.0 25.0 39.0 80-81 32.440033822038444 35.0 33.0 37.0 25.0 39.0 82-83 32.16273200168463 35.0 33.0 36.0 25.0 38.5 84-85 31.89267656960385 35.0 33.0 36.0 24.0 37.0 86-87 31.6208285924916 35.0 32.5 35.5 24.0 37.0 88-89 31.349045823671315 35.0 32.0 35.0 23.0 36.5 90-91 31.080460346097144 35.0 32.0 35.0 20.5 36.0 92-93 30.840275200560434 35.0 32.0 35.0 20.0 36.0 94-95 30.58510472940572 34.5 32.0 35.0 18.5 36.0 96-97 30.34574155344125 34.0 32.0 35.0 13.5 35.5 98-99 30.064675697667614 34.0 31.0 35.0 2.0 35.0 100-101 29.454660034736534 34.0 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.40280693333847495 1101 2 0.4422135289193463 1101 3 0.3987975374699815 1101 4 0.300371646517128 1101 5 0.3248563261323625 1101 6 0.3246953914585333 1101 7 0.3076405494086316 1101 8 0.30952447762346225 1101 9 0.3832486173363847 1101 10-11 0.3804185202005641 1101 12-13 0.34963686442548436 1101 14-15 0.45218926105492585 1101 16-17 0.4457776306380552 1101 18-19 0.4487685178508585 1101 20-21 0.3693483378833804 1101 22-23 0.3784452131161302 1101 24-25 0.3179379094956687 1101 26-27 0.25505084682083634 1101 28-29 0.35511355040882364 1101 30-31 0.2677864494292237 1101 32-33 0.1863079237110128 1101 34-35 0.2460442995394132 1101 36-37 0.17722082526861982 1101 38-39 0.29504638729254395 1101 40-41 0.3347074284185041 1101 42-43 0.4019366661796724 1101 44-45 0.3411060889549873 1101 46-47 0.3736551946847584 1101 48-49 0.2930772793348453 1101 50-51 0.31218623156401293 1101 52-53 0.3590902025836584 1101 54-55 0.3693498351966724 1101 56-57 0.32359480771501126 1101 58-59 0.30527948455547005 1101 60-61 0.34773782108815965 1101 62-63 0.31747885930219155 1101 64-65 0.210125948474861 1101 66-67 0.20334568570407185 1101 68-69 0.15881306910464588 1101 70-71 0.16195363050977818 1101 72-73 0.10832257843865989 1101 74-75 0.13558625013634895 1101 76-77 0.1848413159089013 1101 78-79 0.1955854298654387 1101 80-81 0.16634365827026443 1101 82-83 0.14445510177721133 1101 84-85 0.08451726535189863 1101 86-87 0.1430215243139159 1101 88-89 0.1435873171654194 1101 90-91 0.12182604003212205 1101 92-93 0.1009221099056461 1101 94-95 0.08583322396783188 1101 96-97 0.06110380292935602 1101 98-99 -0.024918299118485976 1101 100-101 -0.12100532430488187 1102 1 0.3341005850700256 1102 2 0.32788553909252727 1102 3 0.31134469329392545 1102 4 0.2744612108764457 1102 5 0.28734932526742796 1102 6 0.2783083197483478 1102 7 0.3076255268424646 1102 8 0.28948227975310914 1102 9 0.3825158899822867 1102 10-11 0.3023713565049775 1102 12-13 0.3025009504495131 1102 14-15 0.3532109554648031 1102 16-17 0.34144401669579594 1102 18-19 0.3508549450839453 1102 20-21 0.35739987974483967 1102 22-23 0.2857007769892803 1102 24-25 0.3455805686376294 1102 26-27 0.26689382041867304 1102 28-29 0.35611865468041515 1102 30-31 0.27475068137484726 1102 32-33 0.3236006987283133 1102 34-35 0.229532145709058 1102 36-37 0.20327371627814728 1102 38-39 0.28154218139068377 1102 40-41 0.24949647138667075 1102 42-43 0.23712488102830775 1102 44-45 0.15594686855995832 1102 46-47 0.18455365427712422 1102 48-49 -0.005887902343282292 1102 50-51 0.1139091802635761 1102 52-53 0.11464796084831619 1102 54-55 0.13396420761230132 1102 56-57 0.06849783907742335 1102 58-59 0.04020851171412687 1102 60-61 0.10542249994679764 1102 62-63 0.0017512302737117125 1102 64-65 -0.041682350697897164 1102 66-67 -0.017813853250437717 1102 68-69 -0.031918442254927015 1102 70-71 -0.055499197224790464 1102 72-73 -0.06626224186408791 1102 74-75 -0.04455164936340594 1102 76-77 -0.0497565391163981 1102 78-79 -0.03710711557010882 1102 80-81 0.029316897539359843 1102 82-83 0.030668926587644307 1102 84-85 -8.13737073759313E-4 1102 86-87 -0.0701418652824195 1102 88-89 -0.13569838166014847 1102 90-91 -0.11645946084242098 1102 92-93 -0.09123163531582179 1102 94-95 -0.13578342217062556 1102 96-97 -0.1688379912709017 1102 98-99 -0.20481790963762592 1102 100-101 -0.17880303727571345 1103 1 0.3363391966896785 1103 2 0.35170853308432726 1103 3 0.287104676463386 1103 4 0.2746789450403142 1103 5 0.2636695740416144 1103 6 0.2940777939603336 1103 7 0.2594501662234805 1103 8 0.2985395953049945 1103 9 0.3570635014655963 1103 10-11 0.31076618232603437 1103 12-13 0.27301464312935053 1103 14-15 0.35427002320915335 1103 16-17 0.39265143518769463 1103 18-19 0.31935179122780255 1103 20-21 0.2930296700093109 1103 22-23 0.25501103050123675 1103 24-25 0.3083143281262508 1103 26-27 0.31565830802814077 1103 28-29 0.27188029923377854 1103 30-31 0.28292609616939046 1103 32-33 0.33718325383614456 1103 34-35 0.26836792964814293 1103 36-37 0.2815980996796341 1103 38-39 0.3844909701347561 1103 40-41 0.43892974083154 1103 42-43 0.4833799630507585 1103 44-45 0.4273316075362956 1103 46-47 0.5370188293873781 1103 48-49 0.41911364230518444 1103 50-51 0.5411436287492535 1103 52-53 0.5394149704591911 1103 54-55 0.4908731332038414 1103 56-57 0.4485988390938047 1103 58-59 0.42951555209929637 1103 60-61 0.3407085785170949 1103 62-63 0.4028763470575214 1103 64-65 0.3598106117031037 1103 66-67 0.255099442761086 1103 68-69 0.28990348705809055 1103 70-71 0.21445250139191785 1103 72-73 0.29373656111397395 1103 74-75 0.3174395244056143 1103 76-77 0.3330418234401371 1103 78-79 0.37291785291454715 1103 80-81 0.3606638580903905 1103 82-83 0.37341487686763486 1103 84-85 0.31178064288241814 1103 86-87 0.3779192493952479 1103 88-89 0.28006110232020376 1103 90-91 0.3551651533340596 1103 92-93 0.3329089693582681 1103 94-95 0.2698591625557647 1103 96-97 0.2579099090713477 1103 98-99 0.3103474748095749 1103 100-101 0.27047899463807923 1104 1 0.31285166374624396 1104 2 0.24953018139746774 1104 3 0.2975510358783211 1104 4 0.218232383568143 1104 5 0.20160232889254104 1104 6 0.2292084389345348 1104 7 0.24840574231736667 1104 8 0.2680879474782927 1104 9 0.3403316651020276 1104 10-11 0.2819468327258363 1104 12-13 0.23140737755005603 1104 14-15 0.3265883057724892 1104 16-17 0.33812055098770344 1104 18-19 0.31895759251572997 1104 20-21 0.28990040201779976 1104 22-23 0.3316988009242934 1104 24-25 0.2987177940315888 1104 26-27 0.2615045349833238 1104 28-29 0.274529681275375 1104 30-31 0.2431105918931422 1104 32-33 0.31449740170961604 1104 34-35 0.2690821146901712 1104 36-37 0.2770435174080319 1104 38-39 0.31811186085796095 1104 40-41 0.3020919616152469 1104 42-43 0.336113036703118 1104 44-45 0.3648518288513358 1104 46-47 0.38646310960834285 1104 48-49 0.37328013747265487 1104 50-51 0.37305470360647774 1104 52-53 0.3231373887101938 1104 54-55 0.2581417308891716 1104 56-57 0.21476198410231007 1104 58-59 0.2714177339433306 1104 60-61 0.27633002302681575 1104 62-63 0.29004396601248317 1104 64-65 0.3367720324898045 1104 66-67 0.3725437179929898 1104 68-69 0.3368957028652062 1104 70-71 0.2556340747692545 1104 72-73 0.28032663311869044 1104 74-75 0.24554113425303825 1104 76-77 0.1709968420057031 1104 78-79 0.2983301681787296 1104 80-81 0.25396846686641084 1104 82-83 0.3125188777257577 1104 84-85 0.2645013062288655 1104 86-87 0.1804420962071731 1104 88-89 0.23017541433400268 1104 90-91 0.18856334454756407 1104 92-93 0.07832782030113705 1104 94-95 0.04119478587117342 1104 96-97 -0.06946192489747105 1104 98-99 -0.04317811224778367 1104 100-101 0.04906722567681143 1105 1 0.29665223248085226 1105 2 0.30323891347769205 1105 3 0.27313304439419994 1105 4 0.19952831627413303 1105 5 0.2521899224380064 1105 6 0.265147453438658 1105 7 0.2699522112770083 1105 8 0.2874577381895307 1105 9 0.2800900527872372 1105 10-11 0.27844748628025684 1105 12-13 0.23938625069995823 1105 14-15 0.3205865028220174 1105 16-17 0.351473433438116 1105 18-19 0.3367158373210444 1105 20-21 0.2963553340075791 1105 22-23 0.23566877314141976 1105 24-25 0.29409823292712645 1105 26-27 0.34342665696763675 1105 28-29 0.30649309312605766 1105 30-31 0.29346897219218704 1105 32-33 0.3450862908608556 1105 34-35 0.3494773894827077 1105 36-37 0.29573102637586857 1105 38-39 0.367454371197077 1105 40-41 0.30673655697152924 1105 42-43 0.387020535899417 1105 44-45 0.3979507120270469 1105 46-47 0.3637945491777117 1105 48-49 0.3102930214850588 1105 50-51 0.27911023787131484 1105 52-53 0.32389389534533564 1105 54-55 0.40852457028793054 1105 56-57 0.29076097788885136 1105 58-59 0.35526452674388764 1105 60-61 0.36531077480509566 1105 62-63 0.33420114721021577 1105 64-65 0.33186341204962133 1105 66-67 0.3098969709624413 1105 68-69 0.22545432327511605 1105 70-71 0.21032946513709305 1105 72-73 0.3371205113208475 1105 74-75 0.39801501478002166 1105 76-77 0.32885139148906717 1105 78-79 0.4657557934447354 1105 80-81 0.4178190980504297 1105 82-83 0.46639049893422424 1105 84-85 0.402618573081881 1105 86-87 0.42149142791070915 1105 88-89 0.4240738679013667 1105 90-91 0.5035573478301707 1105 92-93 0.43688447913968176 1105 94-95 0.40471748954615094 1105 96-97 0.4680821272125222 1105 98-99 0.3998839793563853 1105 100-101 0.5191521726144934 1106 1 0.26708225102813543 1106 2 0.2136427342659175 1106 3 0.21914066761771522 1106 4 0.23931340196068618 1106 5 0.2096581658900618 1106 6 0.24375823744790637 1106 7 0.24150750310113978 1106 8 0.23762238710781958 1106 9 0.30544579125990623 1106 10-11 0.29070387293613464 1106 12-13 0.25769001403174485 1106 14-15 0.3378623848560096 1106 16-17 0.3157089422763377 1106 18-19 0.30922320306677875 1106 20-21 0.3013923141198589 1106 22-23 0.29404134714209107 1106 24-25 0.313961132111892 1106 26-27 0.3307446376191052 1106 28-29 0.34641691113440487 1106 30-31 0.3237563804242072 1106 32-33 0.386870936776198 1106 34-35 0.33665734122122615 1106 36-37 0.37581049589948634 1106 38-39 0.3843575671271324 1106 40-41 0.38822878296731034 1106 42-43 0.4632227354749503 1106 44-45 0.4808688180943079 1106 46-47 0.4628290984296086 1106 48-49 0.4718157798461746 1106 50-51 0.5204220985844614 1106 52-53 0.5147479283429703 1106 54-55 0.49157285723517674 1106 56-57 0.34709104352634057 1106 58-59 0.4685943836297284 1106 60-61 0.518841693271554 1106 62-63 0.49364627700935415 1106 64-65 0.43092323377647546 1106 66-67 0.4408942975867802 1106 68-69 0.39211633739470386 1106 70-71 0.40136871573254496 1106 72-73 0.36212731881117577 1106 74-75 0.37798213182329476 1106 76-77 0.38352300999322964 1106 78-79 0.3524596197939118 1106 80-81 0.4949789083800482 1106 82-83 0.4945789452188407 1106 84-85 0.43214624951686176 1106 86-87 0.38344255622974543 1106 88-89 0.3693306624740913 1106 90-91 0.4446960956068686 1106 92-93 0.3929226019697758 1106 94-95 0.4570938548401138 1106 96-97 0.5421706334496754 1106 98-99 0.588669136207816 1106 100-101 0.6066102200548364 1107 1 0.2409769262969732 1107 2 0.2131116004374931 1107 3 0.2183827342089657 1107 4 0.18081638761807284 1107 5 0.189808126168586 1107 6 0.16371815548013302 1107 7 0.1880285386700038 1107 8 0.17718304586208689 1107 9 0.24547462817718468 1107 10-11 0.19642600712892033 1107 12-13 0.20001045270013407 1107 14-15 0.17099458878321627 1107 16-17 0.2693539170893118 1107 18-19 0.19803929508618268 1107 20-21 0.18715664821537814 1107 22-23 0.22504203491769204 1107 24-25 0.25104172008092007 1107 26-27 0.26850164470793914 1107 28-29 0.24121304397540655 1107 30-31 0.2437116490941591 1107 32-33 0.2748225191910194 1107 34-35 0.24188386698419606 1107 36-37 0.28335205464642854 1107 38-39 0.26604724477218866 1107 40-41 0.2819112916257467 1107 42-43 0.29681593483430646 1107 44-45 0.25992967642171294 1107 46-47 0.26424639100170566 1107 48-49 0.16918376130137602 1107 50-51 0.23439416603123675 1107 52-53 0.20378972700282816 1107 54-55 0.24829647085697815 1107 56-57 0.3177397747716668 1107 58-59 0.3088090610898746 1107 60-61 0.370160833826354 1107 62-63 0.36418510470730325 1107 64-65 0.36109039475415017 1107 66-67 0.402401674028134 1107 68-69 0.42254195826559027 1107 70-71 0.412164816425971 1107 72-73 0.42504472161877516 1107 74-75 0.40003495009296586 1107 76-77 0.3883767792021082 1107 78-79 0.4870441369593834 1107 80-81 0.44277154938497176 1107 82-83 0.4957275512419912 1107 84-85 0.5319400255485647 1107 86-87 0.5394157414259197 1107 88-89 0.5114361783047237 1107 90-91 0.6349138626119313 1107 92-93 0.6168566512220757 1107 94-95 0.6673312613726452 1107 96-97 0.6006287833944661 1107 98-99 0.6494494048035158 1107 100-101 0.855677178462031 1108 1 0.20218804309052274 1108 2 0.21623621436989993 1108 3 0.1783519852604769 1108 4 0.1638511031073122 1108 5 0.15942896450229682 1108 6 0.17514507295471304 1108 7 0.1440087669809884 1108 8 0.16568780791727988 1108 9 0.22567282349287865 1108 10-11 0.16111752353565123 1108 12-13 0.1617118007504601 1108 14-15 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-0.8962512189727363 2316 100-101 -1.243813405678889 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 23.0 4 69.0 5 116.0 6 287.0 7 862.0 8 2161.0 9 4495.0 10 7405.0 11 11285.0 12 15774.0 13 21943.0 14 27584.0 15 35146.0 16 44020.0 17 58470.0 18 50305.0 19 29751.0 20 24510.0 21 23563.0 22 24821.0 23 26902.0 24 32090.0 25 39805.0 26 50013.0 27 63710.0 28 81539.0 29 103485.0 30 130122.0 31 165491.0 32 212488.0 33 280108.0 34 390261.0 35 580241.0 36 921274.0 37 1449978.0 38 1628124.0 39 416245.0 40 165.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.43884246360238 22.177655162015995 16.659830791591457 22.723671582790168 2 14.229664133337819 21.69967701423225 39.789151922945855 24.281506929484074 3 16.62684418907445 26.81113188868111 31.831424169418437 24.730599752826002 4 12.087412933813955 18.73432966819337 38.66146302969981 30.51679436829286 5 12.62695624378187 37.98562160730455 35.498384274961275 13.88903787395231 6 29.265368105362317 39.092693281215645 17.82641411330219 13.81552450011985 7 25.750747307156363 33.80271321134374 22.684073949510232 17.762465531989662 8 25.235479580026965 35.756531936893325 20.416018837503668 18.591969645576036 9 24.901468461424095 16.82879855796703 21.96920438280937 36.300528597799506 10-11 23.44107540382427 27.609634094331923 28.772233017582234 20.177057484261574 12-13 24.436395777119337 25.075142316927707 29.182452598349318 21.30600930760364 14-15 21.656331665780804 26.875363922616145 27.160635397833964 24.307669013769083 16-17 20.41165484266109 30.29954690661784 27.846363512574417 21.44243473814665 18-19 20.456918464059747 28.454724243157347 30.897937453920104 20.1904198388628 20-21 21.605275067841763 27.418979014257104 30.75529478500029 20.220451132900838 22-23 21.15584896810574 27.390523761340813 30.845829911755278 20.607797358798166 24-25 20.985578507878003 27.559572026255196 30.70669704182945 20.74815242403735 26-27 20.812729216900735 27.64793693285354 30.67137112545786 20.867962724787866 28-29 20.71794033331773 27.613224140438852 30.869360740413356 20.79947478583006 30-31 21.00842432575737 27.862521905583566 30.56924141525797 20.559812353401092 32-33 20.731791228504974 27.691171798208465 30.816673069275506 20.760363904011054 34-35 20.93249373910126 27.63542835720675 30.599518397382486 20.832559506309504 36-37 21.258090478187867 27.67696669565106 30.290357773452087 20.774585052708986 38-39 21.038310984989145 27.83464729686349 30.41719585288162 20.709845865265745 40-41 20.93377284753828 27.725163137259468 30.4261207119739 20.914943303228352 42-43 20.92622397225183 27.811364906624426 30.20681858737353 21.05559253375021 44-45 21.030983972503297 27.97548004160424 29.948884760034737 21.044651225857724 46-47 21.30189086062582 28.121097673052937 29.523733588972856 21.05327787734839 48-49 21.25203133141098 28.09120955733565 29.382296230040144 21.274462881213225 50-51 21.2596437031475 28.24133680770743 29.119418141075652 21.379601348069418 52-53 21.284392179553695 28.28352712665548 29.023977034686087 21.40810365910474 54-55 21.310330746069496 28.59847755004616 28.90084663631736 21.190345067566984 56-57 21.28067737451806 28.379336229395253 28.87117499577439 21.468811400312298 58-59 21.139649225326885 28.14961395575591 29.175348297528025 21.53538852138918 60-61 21.305218925810312 28.34867507939055 29.008669258471247 21.337436736327888 62-63 21.183127171852824 28.33981562478004 29.040898146002576 21.43615905736456 64-65 21.224142761655614 28.268164907424364 29.062061685745167 21.445630645174855 66-67 21.11093040701991 28.30076992884525 28.982494087453208 21.605805576681636 68-69 21.379079224267667 28.41786505003397 28.71134551713224 21.491710208566122 70-71 21.34854055505971 28.244828246165515 28.751733240500805 21.654897958273963 72-73 21.18652311348112 28.459016662164842 28.811366768860587 21.543093455493448 74-75 21.290416927922 28.49558556675637 28.63109682951241 21.58290067580922 76-77 21.22329142478519 28.58888795584102 28.578923196292244 21.608897423081547 78-79 21.30056188499792 28.51453487034485 28.697655083480385 21.487248161176847 80-81 21.19801580716899 28.494912550977304 28.674247968888356 21.63282367296536 82-83 21.05455457078302 28.454459109109216 28.842014237003628 21.64897208310414 84-85 21.040360602705064 28.51688074864057 28.664445396335275 21.778313252319084 86-87 21.129830407597954 28.5423540270062 28.586132749827932 21.741682815567913 88-89 20.9877972263345 28.49241639535931 28.648374237277217 21.87141214102897 90-91 20.88472090224182 28.467102118205172 28.680983877662136 21.96719310189087 92-93 20.985589881384005 28.56197751511332 28.53086872955228 21.921563873950394 94-95 20.869048887477835 28.379829876701805 28.752252285285014 21.99886895053535 96-97 20.865017654837416 28.516899475089147 28.62827884153668 21.989804028536756 98-99 20.761447154505447 28.402210652101534 28.61440834946714 22.22193384392588 100-101 20.740375250435484 28.478005784774773 28.46125436768038 22.320364597109364 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1716.0 1 1149.5 2 804.0 3 1098.5 4 994.5 5 919.0 6 1588.0 7 3438.0 8 6064.0 9 7729.5 10 7235.5 11 6420.0 12 6766.0 13 7449.0 14 8039.5 15 8727.5 16 9349.0 17 9939.0 18 10910.5 19 11949.5 20 13477.0 21 16191.5 22 20730.5 23 27955.5 24 36678.0 25 46444.5 26 60564.0 27 78503.0 28 100880.5 29 123273.5 30 139892.5 31 156644.5 32 174659.5 33 191477.0 34 206486.5 35 219437.0 36 228688.0 37 236237.5 38 243103.0 39 246754.0 40 248678.0 41 246236.0 42 239393.5 43 232322.5 44 227314.0 45 221737.5 46 214480.0 47 208096.5 48 203406.0 49 200459.0 50 196138.0 51 187259.5 52 175129.5 53 165494.0 54 159464.5 55 153199.5 56 141510.0 57 126171.5 58 113350.0 59 103000.5 60 91840.5 61 78639.5 62 66819.0 63 56852.0 64 47170.0 65 39664.5 66 32964.5 67 25892.5 68 19468.0 69 14385.5 70 10666.5 71 7458.5 72 5452.5 73 4202.5 74 3184.5 75 2328.5 76 1576.5 77 1134.0 78 836.5 79 574.0 80 381.5 81 245.5 82 164.5 83 117.0 84 83.5 85 51.5 86 36.0 87 26.0 88 17.5 89 15.0 90 14.5 91 9.0 92 4.5 93 4.0 94 3.0 95 3.0 96 2.5 97 1.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05124638424694201 2 0.002171213249519709 3 3.1633570522803705E-4 4 2.0130453969056903E-4 5 0.0025019278504399296 6 0.0017254674830620206 7 0.0 8 0.0 9 1.5816785261401852E-4 10-11 4.313668707655051E-5 12-13 7.189447846091751E-6 14-15 4.816930056881473E-4 16-17 0.0 18-19 6.470503061482576E-5 20-21 4.960719013803308E-4 22-23 0.0 24-25 7.405131281474505E-4 26-27 0.007548920238396338 28-29 0.0074985941034736965 30-31 0.001071227729067671 32-33 0.006333903552406833 34-35 0.008102507722545404 36-37 0.004601246621498721 38-39 0.002437222819825104 40-41 0.002020234844751782 42-43 0.0012653428209121482 44-45 8.267865023005514E-4 46-47 7.548920238396339E-4 48-49 0.0021065082189048834 50-51 0.0014235106735261667 52-53 0.002142455458135342 54-55 8.195970544544597E-4 56-57 2.947673616897618E-4 58-59 8.411653979927349E-4 60-61 0.005880968338103053 62-63 0.002552253985362572 64-65 0.001157501103220772 66-67 0.005147644657801694 68-69 0.0073548051465518625 70-71 0.0025594434332086636 72-73 6.973764410709E-4 74-75 0.0033574721441248477 76-77 0.0018548775442916718 78-79 0.0027176112858226823 80-81 0.0010209015941450286 82-83 1.941150918444773E-4 84-85 8.339759501466432E-4 86-87 0.0049679084616494 88-89 0.0027823163164375077 90-91 0.0013444267472191576 92-93 1.4378895692183504E-4 94-95 4.888824535342391E-4 96-97 3.954196315350463E-4 98-99 5.392085884568813E-4 100-101 2.1568343538275254E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6954637.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.31032426915405 #Duplication Level Percentage of deduplicated Percentage of total 1 70.30990276925942 36.77933813192653 2 16.744181375957513 17.517871147957358 3 5.7145737103924015 8.967936115518281 4 2.510087403484005 5.252139440806689 5 1.3160408521010132 3.44212618624289 6 0.7954452784271799 2.496600027173599 7 0.5177167197199117 1.895735064167793 8 0.3678195721552688 1.5392608873586895 9 0.265377444438269 1.2493782155056739 >10 1.3406956807777146 12.332941670574742 >50 0.07753620480910899 2.7745882101426953 >100 0.037508311838543994 3.535702607954336 >500 0.002177575995068787 0.743575252528248 >1k 8.268535119572488E-4 0.6043594035292978 >5k 2.7561783167226226E-5 0.07462579857993393 >10k+ 8.268534950167868E-5 0.7938218400333428 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22915 0.329492394786385 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20107 0.2891164556827337 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12037 0.17307876744681283 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.8757791384367007E-5 0.0 5 0.0 1.4378895692183503E-5 0.0 2.8757791384367007E-5 0.0 6 0.0 1.4378895692183503E-5 0.0 2.8757791384367007E-5 0.0 7 0.0 1.4378895692183503E-5 0.0 5.7515582768734014E-5 0.0 8 0.0 1.4378895692183503E-5 0.0 5.7515582768734014E-5 0.0 9 0.0 1.4378895692183503E-5 0.0 5.7515582768734014E-5 0.0 10-11 0.0 1.4378895692183503E-5 0.0 5.7515582768734014E-5 0.0 12-13 0.0 5.0326134922642265E-5 0.0 5.7515582768734014E-5 0.0 14-15 0.0 6.470503061482576E-5 0.0 5.7515582768734014E-5 0.0 16-17 0.0 8.627337415310102E-5 0.0 5.7515582768734014E-5 0.0 18-19 0.0 8.627337415310102E-5 0.0 5.7515582768734014E-5 0.0 20-21 0.0 8.627337415310102E-5 0.0 7.908392630700926E-5 0.0 22-23 0.0 8.627337415310102E-5 0.0 1.7254674830620203E-4 0.0 24-25 0.0 8.627337415310102E-5 0.0 3.810407358428628E-4 0.0 26-27 0.0 8.627337415310102E-5 0.0 6.470503061482577E-4 0.0 28-29 0.0 1.1503116553746803E-4 0.0 0.001768604170138571 0.0 30-31 0.0 1.1503116553746803E-4 0.0 0.004507783799499528 0.0 32-33 0.0 1.1503116553746803E-4 0.0 0.00988549078837616 0.0 34-35 0.0 1.1503116553746803E-4 0.0 0.019116741822757966 0.0 36-37 0.0 1.5097840476792677E-4 0.0 0.03502698990615901 0.0 38-39 0.0 1.8692564399838555E-4 0.0 0.05575416804644153 0.0 40-41 7.189447846091752E-6 2.0130453969056903E-4 0.0 0.07906954741131708 0.0 42-43 1.4378895692183503E-5 2.0130453969056903E-4 0.0 0.10277315695988158 0.0 44-45 1.4378895692183503E-5 2.1568343538275254E-4 0.0 0.1317825790188618 0.0 46-47 1.4378895692183503E-5 2.3006233107493606E-4 0.0 0.16854941530377504 0.0 48-49 1.4378895692183503E-5 2.5882012245930305E-4 0.0 0.20711361355021118 0.0 50-51 1.4378895692183503E-5 2.5882012245930305E-4 0.0 0.24788497228539752 0.0 52-53 1.4378895692183503E-5 2.5882012245930305E-4 0.0 0.28826091138904875 0.0 54-55 1.4378895692183503E-5 2.5882012245930305E-4 0.0 0.3327564041085106 0.0 56-57 1.4378895692183503E-5 2.7319901815148656E-4 0.0 0.38711581927281036 0.0 58-59 1.4378895692183503E-5 2.875779138436701E-4 0.0 0.4480679581119762 0.0 60-61 1.4378895692183503E-5 3.0195680953585353E-4 0.0 0.512384758543113 0.0 62-63 1.4378895692183503E-5 3.0195680953585353E-4 0.0 0.5778159233903941 0.0 64-65 1.4378895692183503E-5 3.0195680953585353E-4 0.0 0.6486679319136284 0.0 66-67 1.4378895692183503E-5 3.235251530741288E-4 0.0 0.7314400449656826 0.0 68-69 1.4378895692183503E-5 3.3071460092022056E-4 0.0 0.8238460181314999 0.0 70-71 2.1568343538275254E-5 3.3071460092022056E-4 0.0 0.9253538322704693 0.0 72-73 2.8757791384367007E-5 3.3071460092022056E-4 0.0 1.031822365423242 0.0 74-75 2.8757791384367007E-5 3.379040487663123E-4 0.0 1.1414686345239873 0.0 76-77 2.8757791384367007E-5 3.4509349661240407E-4 0.0 1.266349343610601 0.0 78-79 2.8757791384367007E-5 3.4509349661240407E-4 0.0 1.4111016865438124 0.0 80-81 2.8757791384367007E-5 3.4509349661240407E-4 0.0 1.5681120380546103 0.0 82-83 2.8757791384367007E-5 3.738512879967711E-4 0.0 1.7375241870999163 0.0 84-85 2.8757791384367007E-5 3.738512879967711E-4 0.0 1.9048226384784712 1.4378895692183503E-5 86-87 2.8757791384367007E-5 3.738512879967711E-4 0.0 2.0852777794153745 1.4378895692183503E-5 88-89 2.8757791384367007E-5 3.738512879967711E-4 0.0 2.292182898978049 1.4378895692183503E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13005 0.0 57.3741 1 GTATCAA 24650 0.0 43.322422 1 ATCAACG 31670 0.0 33.265446 3 TCAACGC 32035 0.0 32.916077 4 TATCAAC 32860 0.0 32.133266 2 CAACGCA 33355 0.0 31.727146 5 AACGCAG 35170 0.0 30.142536 6 TGGTATC 5720 0.0 29.39613 2 GTGGTAT 6070 0.0 29.126 1 CGCAGAG 39925 0.0 26.218948 8 ACGCAGA 40135 0.0 26.176432 7 GCAGAGT 43475 0.0 23.739517 9 GTACATG 25220 0.0 22.462482 1 TACATGG 24900 0.0 22.051666 2 GAGTACT 27610 0.0 21.279034 12-13 AGAGTAC 40580 0.0 21.277983 10-11 ACATGGG 25870 0.0 20.63715 3 AGTACTT 28565 0.0 20.600876 12-13 CAGAGTA 42150 0.0 20.502323 10-11 GTACTTT 29305 0.0 19.821356 14-15 >>END_MODULE