FastQCFastQC Report
Wed 25 May 2016
SRR2049403_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049403_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7102389
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370390.5215005824096652No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310460.43712052381248057No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163810.23064070413490448No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77620.10928717083786878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA176900.062.4056361
GTATCAA357900.045.2062451
GTGGTAT64250.036.227511
ATCAACG449700.035.679743
TCAACGC454150.035.2997474
TGGTATC64100.035.1226162
TATCAAC468800.034.2854082
CAACGCA472950.033.8348775
AACGCAG507100.031.7534566
TATAACG7050.030.3172632
CGCAGAG546500.029.0918878
ACGCAGA565750.028.2615437
GCAGAGT575250.027.0433839
GTACATG400150.024.9412781
TACATGG385550.024.281332
GAGTACT355800.022.77642312-13
ACATGGG398500.022.7065723
CAGAGTA574550.022.54943310-11
GTACTTT357550.022.37093514-15
AGAGTAC557100.021.66983610-11
Produced by FastQC (version 0.11.5)