##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049403_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7102389 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.585929466831512 31.0 31.0 34.0 30.0 34.0 2 31.606024113858027 33.0 31.0 34.0 30.0 34.0 3 31.61601835664028 33.0 31.0 34.0 28.0 34.0 4 35.253972290168846 37.0 35.0 37.0 33.0 37.0 5 35.29066402868106 37.0 35.0 37.0 33.0 37.0 6 35.40000568822688 37.0 35.0 37.0 33.0 37.0 7 35.35451001064571 37.0 35.0 37.0 33.0 37.0 8 35.33302949190758 37.0 35.0 37.0 33.0 37.0 9 36.92067950657166 39.0 37.0 39.0 33.0 39.0 10-11 36.851390285719354 39.0 37.0 39.0 33.0 39.0 12-13 36.819723687339575 39.0 37.0 39.0 33.0 39.0 14-15 37.97943149833105 40.0 38.0 41.0 33.0 41.0 16-17 37.971732398774556 40.0 38.0 41.0 33.0 41.0 18-19 38.03397539898195 40.0 38.0 41.0 33.0 41.0 20-21 38.014688649129184 40.0 38.0 41.0 33.0 41.0 22-23 37.90719277696561 40.0 38.0 41.0 33.0 41.0 24-25 37.80591516460166 40.0 38.0 41.0 33.0 41.0 26-27 37.671754109779116 40.0 37.5 41.0 32.5 41.0 28-29 37.52927057360559 40.0 37.0 41.0 32.0 41.0 30-31 37.31858639395843 40.0 37.0 41.0 32.0 41.0 32-33 37.04795527251464 40.0 36.0 41.0 31.5 41.0 34-35 36.850019197765704 39.5 36.0 41.0 31.0 41.0 36-37 36.669399127533 39.0 36.0 41.0 30.5 41.0 38-39 36.45905004639988 39.0 35.5 41.0 30.0 41.0 40-41 36.233503543666785 39.0 35.0 40.5 30.0 41.0 42-43 35.984627355668636 39.0 35.0 40.0 29.5 41.0 44-45 35.64836191033749 39.0 35.0 40.0 28.5 41.0 46-47 35.21308724430611 38.5 35.0 40.0 26.5 41.0 48-49 34.852607833786635 38.0 34.5 40.0 24.5 41.0 50-51 33.478844512740714 37.0 32.5 39.0 23.0 40.0 52-53 33.76186970327871 37.5 33.5 39.0 23.5 40.0 54-55 34.79467718538086 38.5 35.0 40.0 25.5 41.0 56-57 34.9089434555049 39.0 35.0 40.0 25.5 41.0 58-59 34.73590379237183 39.0 35.0 40.0 25.0 41.0 60-61 34.47265645404666 38.0 34.0 40.0 24.0 41.0 62-63 34.203662387402325 38.0 34.0 40.0 23.0 41.0 64-65 33.92428779949958 37.0 34.0 40.0 23.0 41.0 66-67 33.589314243418656 37.0 34.0 40.0 22.0 41.0 68-69 33.23239363543732 36.0 33.0 39.0 21.5 41.0 70-71 32.890941695815314 36.0 33.0 39.0 20.0 41.0 72-73 32.61724885809549 35.0 33.0 39.0 19.5 40.0 74-75 32.41350699039436 35.0 33.0 38.5 19.5 40.0 76-77 32.08193897856059 35.0 33.0 37.0 18.0 39.5 78-79 31.71658015352299 35.0 32.5 37.0 16.5 39.0 80-81 31.384357573205296 35.0 32.0 36.5 12.5 39.0 82-83 31.057076780784605 35.0 32.0 36.0 8.5 37.5 84-85 30.754841786334147 35.0 32.0 36.0 7.0 37.0 86-87 30.479295698954253 35.0 32.0 35.0 2.0 37.0 88-89 30.201838846055885 35.0 31.0 35.0 2.0 36.0 90-91 29.949313322601732 35.0 31.0 35.0 2.0 36.0 92-93 29.717668871699367 34.0 31.0 35.0 2.0 36.0 94-95 29.464066034682133 34.0 31.0 35.0 2.0 35.5 96-97 29.223309508955367 34.0 31.0 35.0 2.0 35.0 98-99 28.915755107189987 34.0 30.0 35.0 2.0 35.0 100-101 28.390894176593257 34.0 29.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3262255051360654 1101 2 0.37326155212314305 1101 3 0.3370447283442566 1101 4 0.3161328257845355 1101 5 0.302834762056456 1101 6 0.3301415430313739 1101 7 0.3302750219758579 1101 8 0.31621359314407016 1101 9 0.3520512463962575 1101 10-11 0.37610501683627007 1101 12-13 0.33344616696523843 1101 14-15 0.5024199615696574 1101 16-17 0.5016978419388209 1101 18-19 0.4511002502939263 1101 20-21 0.3334300172163722 1101 22-23 0.39852475269402277 1101 24-25 0.42291797184496716 1101 26-27 0.3493157030257876 1101 28-29 0.3316309067699805 1101 30-31 0.3432849602360548 1101 32-33 0.31864632071426513 1101 34-35 0.350739764897547 1101 36-37 0.29793935188350673 1101 38-39 0.2580302242021659 1101 40-41 0.3014814065788016 1101 42-43 0.2051449509065293 1101 44-45 0.11762491886913296 1101 46-47 0.08302079663249629 1101 48-49 0.07569481809237999 1101 50-51 -0.07700348031820425 1101 52-53 0.12277724804899748 1101 54-55 0.31910000383594905 1101 56-57 0.39238103432457905 1101 58-59 0.32166114746002705 1101 60-61 0.4106287819141201 1101 62-63 0.3101492638567933 1101 64-65 0.3010686028570575 1101 66-67 0.2721912929298824 1101 68-69 0.25186214806313956 1101 70-71 0.16828962650659918 1101 72-73 0.16822329706007366 1101 74-75 0.1470298766915974 1101 76-77 0.08984385049722476 1101 78-79 0.013343374308359301 1101 80-81 -0.10819714160257021 1101 82-83 -0.058344955925701214 1101 84-85 -0.1094842771606288 1101 86-87 -0.1487381962313883 1101 88-89 -0.1325822424471994 1101 90-91 -0.2257047984235392 1101 92-93 -0.29706396966579973 1101 94-95 -0.44761982726122795 1101 96-97 -0.4908381184753807 1101 98-99 -0.5920822376503061 1101 100-101 -0.6579165450938866 1102 1 0.37858034345980585 1102 2 0.38057731661936955 1102 3 0.37831644370151807 1102 4 0.3534226056299943 1102 5 0.3959714073645486 1102 6 0.4090821714371984 1102 7 0.3803363288881414 1102 8 0.4066406335886299 1102 9 0.5198786823362056 1102 10-11 0.5253209252991979 1102 12-13 0.45721915420877224 1102 14-15 0.5878878020748317 1102 16-17 0.48398067412332324 1102 18-19 0.47252443248387266 1102 20-21 0.4217989662138777 1102 22-23 0.4271817490370253 1102 24-25 0.4507841939069124 1102 26-27 0.5021168552151707 1102 28-29 0.46204479821783195 1102 30-31 0.44158900172945437 1102 32-33 0.41553589933678126 1102 34-35 0.43945866635242936 1102 36-37 0.39783491193220044 1102 38-39 0.30452381178741206 1102 40-41 0.35459224384743493 1102 42-43 0.3041567251836099 1102 44-45 0.3626732106674737 1102 46-47 0.3102662128569307 1102 48-49 0.20873853166985867 1102 50-51 0.17156526734240884 1102 52-53 0.30968903981629836 1102 54-55 0.42840858196110077 1102 56-57 0.41032804751402807 1102 58-59 0.4636519215132111 1102 60-61 0.4633147037710259 1102 62-63 0.41662953153116433 1102 64-65 0.3982427193874827 1102 66-67 0.33831746062971746 1102 68-69 0.3375479155275798 1102 70-71 0.340236014150193 1102 72-73 0.31821173756512877 1102 74-75 0.3486246881932118 1102 76-77 0.3144050824921294 1102 78-79 0.29100963249015876 1102 80-81 0.23627792686280102 1102 82-83 0.22404493689402116 1102 84-85 0.18071559556528527 1102 86-87 0.1580941546303336 1102 88-89 0.08923806181420701 1102 90-91 0.10636299525115334 1102 92-93 0.020371748091100983 1102 94-95 -0.07004617037598138 1102 96-97 -0.08928828018866852 1102 98-99 -0.16676443699661192 1102 100-101 -0.2921403868228083 1103 1 0.2828543945742794 1103 2 0.29262044571139967 1103 3 0.3608527594002098 1103 4 0.32127619360092297 1103 5 0.3007028664370992 1103 6 0.2781564663671219 1103 7 0.32177325274977875 1103 8 0.27785908652452207 1103 9 0.44070716810887234 1103 10-11 0.4111133202463719 1103 12-13 0.33125408839235604 1103 14-15 0.4387174730615371 1103 16-17 0.4608477407291858 1103 18-19 0.4090985924898902 1103 20-21 0.3559962006439221 1103 22-23 0.36482485569464984 1103 24-25 0.4079443046821041 1103 26-27 0.4052664345171024 1103 28-29 0.33815003675803723 1103 30-31 0.4097879208565871 1103 32-33 0.3273335092256957 1103 34-35 0.35753348722108313 1103 36-37 0.45604978911969596 1103 38-39 0.411253965008072 1103 40-41 0.3716273674536126 1103 42-43 0.3371031884308664 1103 44-45 0.26121721755865224 1103 46-47 0.30630920649592497 1103 48-49 0.2744534220851804 1103 50-51 0.14469411598106063 1103 52-53 0.31373638700041084 1103 54-55 0.40796343885180164 1103 56-57 0.49761212956047274 1103 58-59 0.4897797320313444 1103 60-61 0.4816708013268354 1103 62-63 0.439677923185144 1103 64-65 0.3607924745667077 1103 66-67 0.40478655987763545 1103 68-69 0.3881839098427875 1103 70-71 0.2699351814315136 1103 72-73 0.33379432846417245 1103 74-75 0.30346663672741414 1103 76-77 0.23829262684505892 1103 78-79 0.27083398800358083 1103 80-81 0.21356116232778888 1103 82-83 0.2014756468478076 1103 84-85 0.19394680648364826 1103 86-87 0.15628146500931805 1103 88-89 0.1848158801103139 1103 90-91 0.11953359093761051 1103 92-93 0.07011990069164042 1103 94-95 0.09381021977954873 1103 96-97 0.07152586661067062 1103 98-99 0.018200329341613752 1103 100-101 -0.022821842561942418 1104 1 0.08731050628590964 1104 2 0.12102837847264425 1104 3 0.09770436205622701 1104 4 0.09703935788143525 1104 5 0.03979046653416418 1104 6 0.058985920824341065 1104 7 0.007249858163298484 1104 8 0.028629640891288943 1104 9 0.056433271658320905 1104 10-11 0.03890180935977838 1104 12-13 0.0237025232382706 1104 14-15 0.06424989017124005 1104 16-17 0.1148002235775607 1104 18-19 0.04957266100812063 1104 20-21 0.034972397948195066 1104 22-23 0.022899241876288556 1104 24-25 0.022644178940332438 1104 26-27 0.01113989859245379 1104 28-29 -0.02773225130091106 1104 30-31 -0.011178525945823026 1104 32-33 -0.014289662555206917 1104 34-35 -0.02238996472129884 1104 36-37 -0.07842860315049904 1104 38-39 -0.04248674223421034 1104 40-41 -0.060001981115796355 1104 42-43 -0.07209956465201373 1104 44-45 -0.10749426023799913 1104 46-47 -0.12723075511974713 1104 48-49 -0.17636610539049258 1104 50-51 -0.23377315806981613 1104 52-53 -0.19384694449459516 1104 54-55 -0.09204273793671547 1104 56-57 -0.14296098817353453 1104 58-59 -0.1046576287151666 1104 60-61 -0.19356424753597423 1104 62-63 -0.14527277280655682 1104 64-65 -0.1690052356095535 1104 66-67 -0.1748931295181393 1104 68-69 -0.17954082598920706 1104 70-71 -0.2662427100776128 1104 72-73 -0.30049236276258284 1104 74-75 -0.2790645229646316 1104 76-77 -0.238549269429015 1104 78-79 -0.1868684672698535 1104 80-81 -0.17124923232487532 1104 82-83 -0.2363060036783189 1104 84-85 -0.17818771514614085 1104 86-87 -0.18522920098084583 1104 88-89 -0.2116591590461141 1104 90-91 -0.33512282520145575 1104 92-93 -0.3518365970692727 1104 94-95 -0.35027815241608096 1104 96-97 -0.369586837719698 1104 98-99 -0.32700648277371513 1104 100-101 -0.36223328285666057 1105 1 0.21137660948909698 1105 2 0.19090849289672462 1105 3 0.18239303766243609 1105 4 0.22059173668777277 1105 5 0.21102006708107268 1105 6 0.24023297524452403 1105 7 0.23762525011159852 1105 8 0.2429437647794188 1105 9 0.31873160685670854 1105 10-11 0.24732792447895946 1105 12-13 0.24309918064967206 1105 14-15 0.2662023560559845 1105 16-17 0.2312508428654283 1105 18-19 0.30938544243750243 1105 20-21 0.2872006884998015 1105 22-23 0.2637252263778649 1105 24-25 0.29962547999863887 1105 26-27 0.28745896456198494 1105 28-29 0.33380145529832816 1105 30-31 0.32058280665930994 1105 32-33 0.2521789834901753 1105 34-35 0.35030209472116525 1105 36-37 0.27353016446091516 1105 38-39 0.252564817347654 1105 40-41 0.26108835127163843 1105 42-43 0.23914466353922847 1105 44-45 0.2596823561548902 1105 46-47 0.2987889788328033 1105 48-49 0.1923309266872053 1105 50-51 0.21605946425052025 1105 52-53 0.2310930172367378 1105 54-55 0.3738077851065995 1105 56-57 0.38225645958897303 1105 58-59 0.44683910775426483 1105 60-61 0.3669206416873081 1105 62-63 0.41387303769096917 1105 64-65 0.37874344389226167 1105 66-67 0.4088932152130411 1105 68-69 0.33114839194741563 1105 70-71 0.29730691888436667 1105 72-73 0.3733347522305621 1105 74-75 0.2613074316782118 1105 76-77 0.25772062397632567 1105 78-79 0.36624928335744755 1105 80-81 0.2825652983110629 1105 82-83 0.3733385598411765 1105 84-85 0.3359258228723654 1105 86-87 0.3050603538117116 1105 88-89 0.3191905810554019 1105 90-91 0.375615183376528 1105 92-93 0.4118163038255709 1105 94-95 0.3621247341566587 1105 96-97 0.2727350430210791 1105 98-99 0.25309661729388466 1105 100-101 0.29143520048959104 1106 1 0.138251436071112 1106 2 0.21438608834357709 1106 3 0.23541041701987453 1106 4 0.15525790720673882 1106 5 0.2039198924461516 1106 6 0.1669225679186752 1106 7 0.1983976525823934 1106 8 0.21068423663834324 1106 9 0.2188788769834602 1106 10-11 0.260914390314575 1106 12-13 0.253111870012944 1106 14-15 0.25197973133888496 1106 16-17 0.27701228184250937 1106 18-19 0.31497128784651096 1106 20-21 0.2748226847818316 1106 22-23 0.27438211747278274 1106 24-25 0.31480049471075233 1106 26-27 0.28840293917576787 1106 28-29 0.33417539051858114 1106 30-31 0.2965673814278986 1106 32-33 0.28690588039351894 1106 34-35 0.3043938880566799 1106 36-37 0.25154166164836766 1106 38-39 0.3211998356345376 1106 40-41 0.3691323419558472 1106 42-43 0.35454785257326904 1106 44-45 0.3731469986011078 1106 46-47 0.3422339623371329 1106 48-49 0.26648105724118665 1106 50-51 0.23890871441617634 1106 52-53 0.41238219365423134 1106 54-55 0.4543611324338528 1106 56-57 0.42172689005989383 1106 58-59 0.4173659304876267 1106 60-61 0.389039759622392 1106 62-63 0.38931448127139134 1106 64-65 0.4398909221014904 1106 66-67 0.4784937480509157 1106 68-69 0.5261608963824429 1106 70-71 0.5095181891602678 1106 72-73 0.5346045677310158 1106 74-75 0.5416133496035442 1106 76-77 0.53695291692528 1106 78-79 0.5760498191449983 1106 80-81 0.46777524955954775 1106 82-83 0.4622303081978565 1106 84-85 0.5135291673836697 1106 86-87 0.3911474781676283 1106 88-89 0.45343677342494004 1106 90-91 0.47820144112966645 1106 92-93 0.38918448762468216 1106 94-95 0.4171510589210641 1106 96-97 0.4930867570498805 1106 98-99 0.4306850128022077 1106 100-101 0.5005112302208552 1107 1 0.2221352356966264 1107 2 0.1866319150634439 1107 3 0.19375542882385588 1107 4 0.22787989208184456 1107 5 0.25609705272928096 1107 6 0.278582449560993 1107 7 0.24466808749235014 1107 8 0.2939932475606284 1107 9 0.29371590940274217 1107 10-11 0.2981935818123844 1107 12-13 0.27488793814183055 1107 14-15 0.3760997892106275 1107 16-17 0.322416198367101 1107 18-19 0.3463331616539662 1107 20-21 0.34461677162122584 1107 22-23 0.3427032409557711 1107 24-25 0.3173009871003245 1107 26-27 0.37850122622448623 1107 28-29 0.3551715169054077 1107 30-31 0.4199493933747718 1107 32-33 0.4504664173521462 1107 34-35 0.49641578632171246 1107 36-37 0.5202608761600018 1107 38-39 0.5540305435585537 1107 40-41 0.5482393184551171 1107 42-43 0.5380097289206489 1107 44-45 0.5818755339805151 1107 46-47 0.4702332591038214 1107 48-49 0.42215799524347375 1107 50-51 0.4911545548754148 1107 52-53 0.5270578221824636 1107 54-55 0.5763575560368324 1107 56-57 0.6178463997676147 1107 58-59 0.5578592784753127 1107 60-61 0.5867505120144543 1107 62-63 0.6353000116276846 1107 64-65 0.6682468912444861 1107 66-67 0.7168787477553238 1107 68-69 0.7407316006287559 1107 70-71 0.6994917783638854 1107 72-73 0.68104177454385 1107 74-75 0.7018265100999699 1107 76-77 0.7191226791107681 1107 78-79 0.6320797236050879 1107 80-81 0.6247664611112 1107 82-83 0.6596838186013123 1107 84-85 0.656697460718334 1107 86-87 0.5815324503190205 1107 88-89 0.6854532375806102 1107 90-91 0.6689547956914517 1107 92-93 0.6097261581160929 1107 94-95 0.5850678603821606 1107 96-97 0.6373408789373158 1107 98-99 0.71546291354122 1107 100-101 0.7508230026836991 1108 1 0.1282951910981609 1108 2 0.16819084370173343 1108 3 0.19282679677369785 1108 4 0.1479679067982289 1108 5 0.17958282309987084 1108 6 0.199472553721165 1108 7 0.19473909002875445 1108 8 0.22280256137381116 1108 9 0.20094168290742687 1108 10-11 0.23399967034922042 1108 12-13 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-0.7805326865515845 2316 86-87 -0.6183529134998373 2316 88-89 -0.7154220247814678 2316 90-91 -0.7341479786102063 2316 92-93 -0.8253289806265407 2316 94-95 -1.082262529316111 2316 96-97 -0.8195033136652405 2316 98-99 -0.6594721847333211 2316 100-101 -0.5745993802941349 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 53019.0 3 11519.0 4 9453.0 5 8466.0 6 8786.0 7 9496.0 8 10882.0 9 12860.0 10 15937.0 11 19551.0 12 25010.0 13 30262.0 14 34716.0 15 45241.0 16 59706.0 17 80383.0 18 62532.0 19 33754.0 20 28760.0 21 28626.0 22 29468.0 23 31871.0 24 36843.0 25 43722.0 26 52651.0 27 63946.0 28 78739.0 29 97694.0 30 122583.0 31 157651.0 32 207321.0 33 282245.0 34 402340.0 35 617523.0 36 981176.0 37 1497531.0 38 1489476.0 39 320511.0 40 139.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.16673214680507 20.790676191562497 15.091679234628714 20.950912427003725 2 15.95964559653341 20.694444933346944 40.32503176888444 23.0208777012352 3 18.112891298434104 27.0073572356664 30.944052793220735 23.935698672678765 4 12.06118399199766 17.82570720634397 39.975524938808825 30.137583862849542 5 12.124055042332133 38.6915970752601 35.36323405381736 13.821113828590418 6 28.336231159203567 39.25831709818645 18.215398297576584 14.190053445033396 7 26.21067999536486 34.21810691766251 22.542964790547924 17.028248296424707 8 26.06227181012913 34.456519137324364 20.320990018431196 19.160219034115308 9 26.10826700581895 16.400028275206754 22.16907165511908 35.32263306385522 10-11 23.865120765926875 27.2738242935426 28.601367328280304 20.259687612250225 12-13 25.031707058462228 25.603638042612424 28.49316596193238 20.871488936992964 14-15 22.411981848295646 26.75467109012026 27.493743789726565 23.339603271857523 16-17 20.282219791276958 30.2520703125495 28.09880867880315 21.36690121737039 18-19 20.18677296576498 28.203896355422277 31.689971633032126 19.919359045780624 20-21 21.913184424016254 27.03405212781183 31.483872584657586 19.568890863514326 22-23 21.326064725583446 26.915193803492183 31.87821787322261 19.880523597701767 24-25 20.709625415709787 27.52022199256406 31.494336096060476 20.275816495665676 26-27 20.068790449958847 27.757665340240774 31.62748542935564 20.54605878044474 28-29 20.544846665428107 27.44792077696772 31.950120302095293 20.05711225550888 30-31 20.830989036858817 28.005219122025064 31.219334067738764 19.94445777337735 32-33 20.22351233774165 27.554293208847707 31.894025767032264 20.32816868637838 34-35 20.918653221475143 27.47004776573788 31.543317897181133 20.067981115605843 36-37 21.17580253442521 27.42554821472144 30.93930668189558 20.459342568957776 38-39 20.694657229095395 27.805473222503686 31.338557310914528 20.1613122374864 40-41 20.624994323923097 27.561290542413303 31.40010671376584 20.41360841989776 42-43 20.550258311768925 27.561414826038817 31.262999981625345 20.625326880566913 44-45 20.75292565437359 27.759795714625668 30.775377613938943 20.7119010170618 46-47 21.386529458176387 28.033824846259343 29.94841022349515 20.631235472069125 48-49 21.337029378532584 27.937376280544978 29.707974740374393 21.017619600548045 50-51 21.319847487269193 28.114692772682826 29.283608781767356 21.28185095828063 52-53 21.169886564860665 28.39843186181909 29.307490126967657 21.124191446352594 54-55 21.22650426262042 29.031870611275867 29.182832477457982 20.558792648645728 56-57 21.39015412512226 28.366544332401507 29.05888554186119 21.184416000615037 58-59 21.421029668811194 27.915093815578974 29.42487597131776 21.239000544292075 60-61 21.52750189815739 28.243906096385214 29.285352983354297 20.943239022103104 62-63 21.064446989027747 28.24207740312641 29.496401307617003 21.197074300228834 64-65 21.201891379775837 28.335052865493864 29.179387156625975 21.283668598104324 66-67 21.109158043067122 28.17871914906559 29.226026074115456 21.48609673375183 68-69 21.51081200076539 28.77366134971087 28.80853059602123 20.906996053502507 70-71 21.689370722683343 28.258895442318483 28.721032730829105 21.33070110416907 72-73 21.363329037076127 28.1991299727159 28.94696454926048 21.49057644094749 74-75 21.292065590426407 28.5379433792202 28.936990925387196 21.233000104966194 76-77 21.46616686132303 28.69453464222574 28.64078090278543 21.1985175936658 78-79 21.483398538567304 28.585765710331057 28.920315378836897 21.010520372264743 80-81 21.487355307806556 28.6101558157276 28.789294089028406 21.11319478743744 82-83 21.303033558929606 28.614883214113867 29.108868213259797 20.973215013696734 84-85 21.120690686965375 28.618044526464352 28.85858217051764 21.402682616052637 86-87 21.386322010516114 28.586365097228477 28.753825969811842 21.273486922443567 88-89 21.22753496762577 28.706988625247487 28.953708768009356 21.11176763911739 90-91 21.00996608228815 28.460246552154555 29.011414649765022 21.51837271579227 92-93 21.146590809811634 28.726559874869135 28.763702644113202 21.363146671206028 94-95 21.176554653735653 28.31615229140399 29.0902249340179 21.41706812084246 96-97 21.20958126662495 28.678905184760488 28.805477286436066 21.30603626217849 98-99 20.88590791706119 28.605980525446974 28.817746770263792 21.69036478722804 100-101 20.98417336532892 28.779228694022596 28.635606221101163 21.60099171954732 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5180.0 1 3216.5 2 1951.0 3 2654.0 4 2114.5 5 1745.5 6 2806.5 7 5601.5 8 9714.0 9 11945.5 10 10051.5 11 7917.0 12 7986.0 13 8605.0 14 9159.0 15 9564.5 16 9922.0 17 10512.0 18 11348.0 19 12397.0 20 13920.5 21 16042.5 22 20158.0 23 27932.5 24 34916.0 25 41260.0 26 53380.5 27 70507.0 28 96308.0 29 115950.5 30 121277.5 31 133652.5 32 155106.0 33 173962.5 34 192245.0 35 216935.5 36 235429.5 37 245596.0 38 258000.0 39 265147.5 40 270299.0 41 268518.0 42 265446.0 43 266928.5 44 262354.5 45 256202.0 46 252228.5 47 248534.5 48 239548.5 49 232577.5 50 230210.5 51 215233.5 52 189388.5 53 166096.5 54 151615.5 55 146946.0 56 132257.5 57 112524.5 58 99562.5 59 86275.0 60 73080.5 61 60881.5 62 50194.0 63 40901.0 64 33161.5 65 27425.0 66 22354.0 67 17414.5 68 13107.5 69 9900.5 70 7515.5 71 5503.0 72 4010.5 73 3063.5 74 2502.5 75 1981.5 76 1415.5 77 1080.0 78 891.0 79 702.5 80 537.5 81 441.5 82 352.5 83 273.0 84 241.0 85 195.5 86 162.0 87 138.5 88 119.0 89 107.5 90 91.5 91 69.5 92 60.0 93 56.0 94 44.5 95 36.5 96 33.0 97 24.5 98 17.5 99 16.0 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014065689727780328 2 0.004421047622145168 3 0.003731138916778566 4 0.006561172585731365 5 0.0030834695199037955 6 0.007222921752103412 7 0.0016895723396733128 8 0.004209851079686004 9 0.010714704587428259 10-11 0.00718068244361158 12-13 0.009264488329208665 14-15 0.003463623296330291 16-17 0.006223258117796702 18-19 0.004090173038959145 20-21 8.095867460934623E-4 22-23 9.29264786820322E-4 24-25 0.007455237948808492 26-27 0.0013375781022413726 28-29 8.518260545852951E-4 30-31 6.335896273774922E-4 32-33 0.0013586977564872891 34-35 0.0017247717634165068 36-37 0.0015206151057059814 38-39 0.0019430081906243095 40-41 0.0029919510181714913 42-43 0.0030341903266633244 44-45 0.007870591148978182 46-47 0.013826333646326608 48-49 0.005399591602205962 50-51 0.005976862151594344 52-53 0.013713695490348388 54-55 0.008384502735628813 56-57 0.005624867914162403 58-59 0.007089163941879274 60-61 0.001499495451460065 62-63 0.0022034839263239455 64-65 0.0023442816212967212 66-67 0.002435800123029026 68-69 0.00199932726861342 70-71 0.002400600699285832 72-73 0.0011686208682740412 74-75 0.0014431763734709547 76-77 0.007849471494732266 78-79 0.006392215351764033 80-81 0.012552114506822986 82-83 0.014924555667114264 84-85 0.008307064003393788 86-87 0.006075420538075287 88-89 0.0029919510181714913 90-91 0.0028159538994555213 92-93 9.081451325744055E-4 94-95 0.001182700637771319 96-97 0.0015910139531923697 98-99 0.006399255236512672 100-101 0.011045579170614283 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7102389.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.500715909825374 #Duplication Level Percentage of deduplicated Percentage of total 1 70.05288408120089 31.174034931614486 2 16.788815290051158 14.942285993701981 3 6.124782216743099 8.176715803105052 4 2.6533546823396246 4.723047317072023 5 1.3950457268163492 3.1040266785135104 6 0.7444652963786419 1.9877543195321934 7 0.46821572336690087 1.4585154423044688 8 0.3278678194129907 1.167228215013714 9 0.22850545856883198 0.9151790846054368 >10 0.9804414660326656 7.895046562835829 >50 0.10689784323053445 3.35703589058185 >100 0.10718196806030725 10.012715276367649 >500 0.013864673147164308 4.244518923269487 >1k 0.007487397097727282 5.386950816773753 >5k 9.51787765455187E-5 0.2624189297730596 >10k+ 9.51787765455187E-5 1.192525814935775 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 37039 0.5215005824096652 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31046 0.43712052381248057 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16381 0.23064070413490448 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7762 0.10928717083786878 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.815953899455521E-5 0.0 5 0.0 0.0 0.0 2.815953899455521E-5 0.0 6 0.0 0.0 0.0 2.815953899455521E-5 0.0 7 0.0 0.0 0.0 2.815953899455521E-5 0.0 8 0.0 0.0 0.0 4.223930849183282E-5 0.0 9 0.0 0.0 0.0 4.223930849183282E-5 0.0 10-11 0.0 0.0 0.0 6.335896273774923E-5 0.0 12-13 0.0 0.0 0.0 9.151850173230444E-5 0.0 14-15 0.0 0.0 0.0 1.4079769497277606E-4 0.0 16-17 0.0 0.0 0.0 1.9711677296188647E-4 0.0 18-19 0.0 0.0 0.0 2.604757356996357E-4 0.0 20-21 0.0 0.0 0.0 3.027150441914685E-4 0.0 22-23 0.0 0.0 0.0 3.94233545923773E-4 0.0 24-25 0.0 0.0 0.0 5.420711256451879E-4 0.0 26-27 0.0 0.0 0.0 8.870254783284892E-4 0.0 28-29 0.0 0.0 0.0 0.0016895723396733128 0.0 30-31 0.0 0.0 0.0 0.00382265741851087 0.0 32-33 0.0 0.0 0.0 0.007800192301491794 1.4079769497277606E-5 34-35 0.0 0.0 0.0 0.01424872673124494 1.4079769497277606E-5 36-37 0.0 0.0 0.0 0.024555118003252146 1.4079769497277606E-5 38-39 0.0 0.0 0.0 0.04175355644417674 1.4079769497277606E-5 40-41 0.0 0.0 0.0 0.05921247062080097 1.4079769497277606E-5 42-43 1.4079769497277606E-5 0.0 0.0 0.07678402295340342 1.4079769497277606E-5 44-45 3.5199423743194016E-5 0.0 0.0 0.0988047824471456 1.4079769497277606E-5 46-47 4.223930849183282E-5 0.0 0.0 0.12597169769214273 1.4079769497277606E-5 48-49 4.223930849183282E-5 0.0 0.0 0.1576230195220228 1.4079769497277606E-5 50-51 4.223930849183282E-5 0.0 0.0 0.19168198193593733 1.4079769497277606E-5 52-53 4.223930849183282E-5 0.0 0.0 0.22412177085766494 1.4079769497277606E-5 54-55 4.223930849183282E-5 0.0 0.0 0.26077141085907857 1.4079769497277606E-5 56-57 4.223930849183282E-5 0.0 0.0 0.3051790038534921 1.4079769497277606E-5 58-59 4.223930849183282E-5 0.0 0.0 0.3584005325532015 1.4079769497277606E-5 60-61 4.223930849183282E-5 0.0 0.0 0.4160783082987992 1.4079769497277606E-5 62-63 4.223930849183282E-5 0.0 0.0 0.47398136035635335 1.4079769497277606E-5 64-65 4.223930849183282E-5 0.0 0.0 0.5350030813575545 1.4079769497277606E-5 66-67 4.223930849183282E-5 0.0 0.0 0.6071407803768563 1.4079769497277606E-5 68-69 4.223930849183282E-5 0.0 0.0 0.693273770276452 1.4079769497277606E-5 70-71 4.223930849183282E-5 0.0 0.0 0.7900158664922465 1.4079769497277606E-5 72-73 4.223930849183282E-5 0.0 0.0 0.8932909757547778 1.4079769497277606E-5 74-75 4.223930849183282E-5 0.0 0.0 0.9978614238110585 1.4079769497277606E-5 76-77 4.223930849183282E-5 0.0 0.0 1.1097040728126832 1.4079769497277606E-5 78-79 4.223930849183282E-5 0.0 0.0 1.2444615466711273 1.4079769497277606E-5 80-81 4.223930849183282E-5 0.0 0.0 1.3984238261238577 1.4079769497277606E-5 82-83 4.223930849183282E-5 0.0 0.0 1.5641215934525694 1.4079769497277606E-5 84-85 4.223930849183282E-5 0.0 0.0 1.730572628449385 1.4079769497277606E-5 86-87 4.223930849183282E-5 0.0 0.0 1.911603264760632 1.4079769497277606E-5 88-89 4.223930849183282E-5 0.0 0.0 2.121145434303866 1.4079769497277606E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 17690 0.0 62.405636 1 GTATCAA 35790 0.0 45.206245 1 GTGGTAT 6425 0.0 36.22751 1 ATCAACG 44970 0.0 35.67974 3 TCAACGC 45415 0.0 35.299747 4 TGGTATC 6410 0.0 35.122616 2 TATCAAC 46880 0.0 34.285408 2 CAACGCA 47295 0.0 33.834877 5 AACGCAG 50710 0.0 31.753456 6 TATAACG 705 0.0 30.317263 2 CGCAGAG 54650 0.0 29.091887 8 ACGCAGA 56575 0.0 28.261543 7 GCAGAGT 57525 0.0 27.043383 9 GTACATG 40015 0.0 24.941278 1 TACATGG 38555 0.0 24.28133 2 GAGTACT 35580 0.0 22.776423 12-13 ACATGGG 39850 0.0 22.706572 3 CAGAGTA 57455 0.0 22.549433 10-11 GTACTTT 35755 0.0 22.370935 14-15 AGAGTAC 55710 0.0 21.669836 10-11 >>END_MODULE