FastQCFastQC Report
Wed 25 May 2016
SRR2049403_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049403_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7102389
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT361480.5089555077875909No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT298220.4198868859478128No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200940.2829188882782962No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76010.10702032794880707No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA162550.062.795131
GTATCAA328950.044.594921
TGGTATC58600.037.1241842
GTGGTAT62350.034.8303951
TATAACG7100.034.1193582
ATCAACG450650.032.4524233
TCAACGC457100.032.046454
TATCAAC465050.031.509722
CAACGCA470350.031.1638855
AACGCAG509750.028.9220496
CGCAGAG566950.025.6103348
ACGCAGA587950.024.8975627
GCAGAGT600500.023.7604439
GTACATG369300.023.2132471
TACATGG355950.022.6988772
AGTACTT383700.021.58849112-13
ACATGGG365950.021.5458263
AGAGTAC581700.021.44190210-11
GAGTACT385550.020.8073412-13
GTACTTT386250.020.45620214-15
Produced by FastQC (version 0.11.5)